观赏植物养护专用缓释肥在3种植物上的应用研究初报

就一种新型观赏植物养护专用缓释肥在醉蝶花、罗汉松、莞香3种观赏植物上的应用效果进行初步探讨。结果表明:缓释肥可促进3种观赏植物的生长,与复合肥相比,该缓释肥具有肥效慢、肥效持续时间长、肥料利用效率高的特点。试验还发现,适量施用缓释肥可促进醉蝶花开花和延长开花期,且该缓释肥覆土施用要明显优于表土施用。     

新型籼型优质不育系浙农3A配组特性评价

以宜香1 B为母本,协青早B为父本杂交,经回交和自交,育成不育度高,农艺性状稳定的籼型优质不育系浙农3 A。经田间纯度鉴定及特异性分子标记检测,结果显示,该不育系纯度极高。与155份自育恢复系材料测配结果表明,该不育系配合力良好,生育期较短,从中筛选出的5个参试组合综合农艺、产量和品质优良,适宜浙江、江西等地连晚种植。     

CD147 monoclonal antibody-mediated nanoparticles for gene therapy to target lung cancer cells

AIM: In this study, CD147 antibody was used to carry out targeted modification of nanoparticles for protein kinase Cε (PKCε)-siRNA gene therapy to target lung cancer cells. The inhibitory effects of the nanoparticles on the proliferation and invasion of the lung cancer cells were observed. METHODS: The magnetic nanoparticles targeting CD147 protein were assembled as gene vector. The expression of CD147 in the lung cancer cells was observed under laser scanning confocal microscope. The cells were divided into CP group, CN group and LP group as the experimental groups. Targeted nanoparticles were used as CA group. Non-transfected cells were used as control group. The cell transfection was carried out with 250 ng plasmids/well in 6-well plate. The effect of nanocontrast agent on the cell endocytosis was observed under laser scanning confocal microscope. The mRNA expression of PKCε was detected by RT-qPCR. The protein expression of Ki67, MMP3, PKCε, Wnt1 and GAPDH was determined by Western blot. The cell proliferation ability was detected with colony formation assay. The cell invasion ability was detected by Transwell method. RESULTS: The expression of CD147 protein in the human lung cancer A549 cells was confirmed by immunofluorescence staining. The endocytosis of siRNA into the A549 cells in CP group was observed with the highest efficiency as compared with CN group and LP group. The relative mRNA expression of PKCε in the A549 cells of CP group, CN group, LP group and CA group were (9.76±0.18)%, (98.51±0.32)%, (99.17±0.16)% and (99.68±0.11)%, respectively. The difference between CP group and control group was statistically significant (P<0.05). No significant difference among CN group, LP group and control group was observed. The protein expression of PKCε, Ki-67, MMP3 and Wnt1 in CP group was significantly reduced, and the protein expression levels among CN group, LP group and control group had no significant difference. The colony number in CP group was significantly smaller than that in control group (P<0.05). The effective colony numbers in CN group, LP group and CA group had no significant difference as compared with control group. The number of the invading cells in CP group was significantly less than that in control group (P<0.05). The numbers of the invading cells in CN group, LP group and CA group had no significant difference as compared with control group. CONCLUSION: Nanogene vector targeting CD147 can carry PKCε-siRNA to conduct gene therapy efficiently on the lung cancer cells to achieve effective inhibitory effects on the proliferation and invasion of the lung cancer cells.     

Development and application of plant transformation techniques

Genetic transformation is a powerful biotechnology for introducing novel genes into economically important plants from distantly-related plants or even unrelated species such as microbes and animals.This feat is impossible to be achieved by conventional breeding techniques.Development of transgenic plants has been a controversial subject since 1971 when the first genetically modified organism(GMO)was developed(James and Krattiger1996).Afterwards,transgenic study of plants has sparked     

马尾松土贡种源纯林与混交林生长量和产脂力比较

对广西土贡马尾松(Pinus massoniana)种源人工成熟纯林和混交林于2007年和2008年进行连续2年采脂试验,结果表明:马尾松混交林胸径、树高、材积年均生长指标均高于马尾松纯林,且树高生长量差异显著;马尾松混交林松脂产量大于纯林,但差异不显著;马尾松混交林与纯林各年产脂力和矫正产脂力无显著差异;胸径和松脂产量关系最为紧密,其次为树高和树冠比,而与枝下高无显著相关性。     

Soil nitrogen and denitrification potential as affected by land use and stand age following agricultural abandonment in a headwater catchment

Changes in vegetation and soil properties because of agricultural abandonment may affect soil nitrogen (N) and associated processes. We investigated soil N (total N: TN, inorganic N: NH₄–N and NO₃–N) and denitrification potential in cropland, pine plantations and abandoned agricultural land along a secondary succession sequence (grassland→shrubland→secondary forest) in a headwater catchment in the Qinling Mountains, northwest China. The results show that the soil denitrification potential differed significantly among the five land‐use types with the highest potential in the secondary forest, followed by grassland, shrubland, cropland and plantations. The denitrification potential of the 20‐ to 40‐cm layer was significantly lower compared with the topsoil (0–20 cm) across all land‐use types. TN, soil organic matter (SOM) and NH₄–N increased significantly with stand age, whereas there was an opposite trend in soil pH. However, the denitrification potential did not relate to stand age in a linear manner. We conclude that changes in soil TN, SOM and pH during vegetation succession following agricultural abandonment are critical controls on the denitrification potential.     

新隆优一号在皖南地区高产制种技术研究

介绍了宣城市的自然气候特征以及新隆优一号亲本特征与特性，分析了高产制种技术要点。     

纳米CuI催化偶联反应合成二苯醚类农药中间体

二苯醚是一种重要的农药中间体,通过W/O微乳法,制备出形貌均一的球状纳米CuI,并应用于催化C-O交叉偶联反应中,在温和的反应条件下,合成了具有优良产率的二苯醚类化合物,该方法可在今后的农药合成中应用。     

南通市通州区兽医服务体系现状及对策

通过对南通市通州区兽医服务体系建设现状进行调查,分析其存在的问题,并提出相应的对策,以期促进兽医服务体系建设。     

一种导航接收机启动策略的优化方案

首次定位时间（TTFF）是导航接收机快速定位能力的重要指标之一，而首次定位时间和导航接收机的冷、温、热启动策略密切相关。基于我国自主研发的北斗卫星导航系统（BDS），根据其特点，提出一种优化启动策略。该启动策略充分利用接收机的硬件资源，合理分配使用各个通道进行卫星信号的搜索，更加充分的使用已知信息，缩小接收机搜索范围和增加捕获卫星信号的成功率。实验结果说明该启动策略可有效缩短导航接收机的首次定位时间。