猎豹与虎猫杯状病毒的分离及其超变区基因比较研究

用F81细胞从上海某动物园患口腔溃疡的猎豹和虎的唾液病料中分离获得两株杯状病毒，经形态学、理化学、生物学鉴定和病毒核酸超变区基因RT-PCR扩增与序列测定证明两株病毒均为猫杯状病毒(FCV)，分别命名为FCV/cheetah/Shanghai/02/2002与FCV／tiger/Shanghai／03/2002。人工感染猫可引起体温升高，口腔溃疡，食欲下降等症状。两株病毒的超变区序列520bp长片段问的同源性为99.2％，与国内桂林虎分离株(TFCV9710)的同源性为74．0％，与国外分离株的总体同源性为58．1％，说明不同宿主或同种不同个体间杯状病毒分离株超变区核酸差异十分显著，符合猫杯状病毒的分子生物学特征。     

羊支原体性肺炎两标准株对抗菌药物敏感性的研究

本实验测定了环丙沙星、氧氟沙星、单诺沙星、红霉素、罗红霉素、泰勒菌素、泰妙菌素、四环素等8种药物对羊肺炎支原体两个标准株Y98和Y-goat的体外抑菌浓度以及红霉素与氧氟沙星、泰勒菌素对Y-goat和四环素与氧氟沙星、泰勒菌素对Y98的联合药敏作用。对羊肺炎支原体两个标准株对抗菌药物的敏感性进行了分析。     

樟子松枯梢病的防治研究

樟子松移植苗枯梢病Sphaeropsis sapinea可以通过药剂有效地进行防治。移植前以50％多菌灵wp500倍液浸苗；在人工林中，内吸性杀菌剂－甲基托布津、多菌灵为首选药剂；在树木新梢生长停止期和展叶中期（辽西北为6月初和6月中旬），70％甲基托布津wp 1000倍液或50％多菌灵wp 500倍液防治2次。保护性杀菌剂－百菌清效果不佳。适时的卫生伐对该病有明显的抑制作用。     

兵团农机产业化的若干思考

农机产业化是社会分工细化、生产专业化深化的必然产物，其实质是传统农业转变为现代产业的过程。日前，兵团农机产业化进程中存在着一些问题。为此，结合兵团实际，提出了推进农机产业化发展的基本思路。     

公共理论课教学状况调查数据的对比分析

为摸清我校公共理论课("两课")教学状况,提高公共理论课教学效果,我们曾分别召开了一系列座谈会,听取学生对公共理论课的意见、建议.同时,为获得更为准确的数据,以便有针对性地提出改进公共理论课教学的对策,我们分别进行了公共理论课教学状况书面问卷调查、网上问卷调查.     

高校公寓文化建设的几点思考

从校园文化、学风建设、素质教育、思想教育等方面，阐述公寓文化的作用和意义，提出理顺管理机制、发挥学生主体作用、加强制度建设，开展公寓文化活动等几点建议。     

The Brief Introduction to the Sino-US Joint Centers for Soil and Water Conservation and Environmental Protection

Erosion and transport of soil has worldwide implications for agriculture, landscape stability, climate, natural hazards, and clean, renewable resources of water and air. Assured access to clean water and a healthy and safe environment requires an ethic of conservation and protection. The minimum scale in which these principles apply successfully is basin wide. These are the fundamental concerns of the Sino-US Centers for Soil and Water Conservation and Environmental Protection.     

Cybrid/hybrid plants regenerated from somatic fusions between male sterile Satsuma mandarin and seedy tangelos

Somatic hybridization provides an alternative for transferring mitochondria-encoded cytoplasmic male sterility (CMS). Herein, symmetric protoplast electrofusion was conducted between embryogenic callus protoplasts of Citrus unshiu Marc. cv. Guoqing No. 1 (G1), a CMS cultivar, and mesophyll-derived protoplasts of seedy ‘Page’ tangelo [C. reticulata Blanco × (C. reticulata Blanco × C. paradisi Macf.)] or ‘Nova’ tangelo [C. reticulata Blanco × (C. reticulata Blanco × C. paradisi Macf.)], to transfer CMS trait. Flow cytometry analysis showed that 14 plants recovered from G1 + ‘Page’ tangelo that displayed typical morphological character of ‘Page’ were diploid, and 6 plants regenerated from G1 + ‘Nova’ tangelo were tetraploid. Genetic compositions of regenerated plants from the two fusions were determined by SSR, CAPS and chloroplast-SSR analysis. Cybrid nature of diploids from G1 + ‘Page’ tangelo with nuclear DNA from ‘Page’, mitochondrial DNA (mtDNA) from the G1 and chloroplast DNA (cpDNA) derived from either parent was confirmed. Tetraploid plants from G1 + ‘Nova’ tangelo were identified as somatic hybrids with random cpDNA inheritance. The regenerated cybrid and hybrid plants hold great potential for Citrus seedless breeding at diploid or triploid levels.     

黄金树花器官发生及发育的形态观察

利用普通光学显微镜和扫描电镜技术对黄金树花器官的发生及发育过程进行了观察.结果显示:(1)黄金树花器官的形态发生及发育过程集中于3月下旬-5月上旬;(2)花原基分化形成花的整个过程符合一般的分化顺序:花萼原基-花冠原基-雄蕊原基-雌蕊原基,且各原基在分化顺序上存在交叉;(3)花药及胚珠的发育与花器官的形态发生之间有明显的连续性,当花蕾直径为3.0 mm左右时花粉母细胞及完整的花粉囊壁形成,直径达到3.5 mm左右时胚珠中出现孢原细胞的分化,它直接起大孢子母细胞的功能.     

基于ProE的香蕉假茎切块还田机构的模拟仿真设计与可行性试验

根据香蕉假茎先分段再进行无支撑切块还田的工艺设计思想，设计了香蕉假茎切块还田机构。该机构采用多把圆盘锯齿刀背刀交错布置于2条旋转轴上的类似对辊式运动原理的切块方法，利用ProE设计软件模拟仿真出合适的刀盘组合，以最快最准确的速度来完成切块机构的设计制作，并对此机构进行可行性试验。结果表明，采用嘲盘锯齿刀背刀交错布置在2条传动轴上的类似对辊式运动原理的切块机理可以实现切块作业。