Assessment of the efficacy of various mulch materials on improving the growth and yield of ginger (Zingiber officinale) under bamboo-based agroforestry system in NW-Himalaya

Agroforestry Systems - Agroforestry as a smart agriculture practice has started to get global attention nowadays. The present investigation is based on bamboo-ginger intercropping research carried...     

Mucosal immunization provides better protection than subcutaneous immunization against <Emphasis Type="Italic">Pasteurella multocida</Emphasis> (B:2) in mice preimmunized with the outer membrane proteins

To investigate the effect of boosting immunity via mucosal route vis-a-vis parenteral route in the mouse model of haemorrhagic septicaemia, mice preimmunized with OMP of Pasteurella multocida (B:2) were immunized with 10² cfu of P. multocida via intranasal and subcutaneous routes. Mice were challenged through intranasal route (natural route of infection) with 10⁸ cfu 14 days after immunization. Group of mice which were immunized intranasally showed significant protection (P < 0.05) of 88% as compared to 50% protection in group of mice immunized subcutaneously. In the control group of mice, 100% mortality occurred within 48 h. of challenge. The results of present study indicated that boosting of immunity via mucosal route in mice preimmunized with OMP provided better protection against P. multocida. This study may have implications for developing better vaccination strategies for the natural host.     

Multi-scale processes influencing global carbon storage and land-carbon-climate nexus: A critical review

Carbon(C) is a key constitutive element in living organisms(plants, microbes, animals, and humans). Carbon is also a basic component of agriculture because it plays a dynamic role in crop growth, development, nutrient cycling, soil fertility, and other agricultural features. The presence of C enhances soil physical, chemical, and biological properties. The C cycle supports all life on the Earth by transferring C between living organisms and the environment. The global climate is changing, and th...     

Role of neuropeptide Y (NPY) in the regulation of reproduction: study based on catfish model

Significance of NPY in the regulation of GnRH–LH axis was evaluated. Considerable NPY immunoreactivity was seen in the components like olfactory system, basal telencephalon, preoptic and tuberal areas, and the pituitary gland that serve as neuroanatomical substrates for processing reproductive information. Close anatomical association as well as colocalizations of NPY and GnRH were seen in the olfactory receptor neurons, olfactory nerve fibers and their terminals in the glomeruli, ganglion cells of nervus terminalis, medial olfactory tracts, fibers in the ventral telencephalon and pituitary. In the pituitary, NPY fibers seem to innervate the GnRH as well as LH cells. Intracranial administration of NPY resulted in significant increase in the GnRH immunoreactivity in all the components of the olfactory system. In the pituitary, NPY augmented the population of GnRH fibers and LH cells. HPLC analysis showed that salmon GnRH content in the olfactory organ, bulb, preoptic area+telencephalon and pituitary was also significantly increased following NPY treatment. NPY may play a role in positive regulation of GnRH throughout the neuraxis and also up-regulate the LH cells in the pituitary.     

Influence of the antibacterial herbs, Solanum trilobatum, Andrographis paniculata and Psoralea corylifolia on the survival, growth and bacterial load of Penaeus monodon post larvae

The indiscriminate use of antibiotics and chemicals in shrimp hatcheries has led to biomagnification and that in turn could lead to rejection of a whole consignment. The application of the bioencapsulation technique as a tool for curative treatment in shrimp larvae was investigated. Herbs having antibacterial properties such as Solanum trilobatum, Andrographis paniculata and Psoralea corylifolia (methanolic extracts) were bioencapsulated in Artemia and fed to Penaeus monodon post larvae PL 1–25. The post larvae were reared in a medium inoculated with pathogenic bacteria such as Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella typhi and Vibrio sp. Post larvae reared in the non-inoculated water and fed with non-enriched Artemia exhibited 90% survival, highest specific growth rate (12.43%) and reduced bacterial load. P. monodon reared in the bacterial inoculated water and fed with the non-enriched Artemia exhibited the lowest survival (10–30%), specific growth rate (8.42–9.1%) and increased bacterial load (2.86 × 10³ to 3.76 × 10⁵ cfu/g). The methanolic extracts of the herbs helped to increase survival and specific growth rate and reduced bacterial load in the P. monodon culture system. Among the three herbal extracts, P. corylifolia enriched Artemia fed post larvae showed the tendency to higher survival (>50%), growth rate (11.5 averaged) and low bacterial load (1.12 × 10⁵ cfu/g). This revised version was published online in August 2006 with corrections to the Cover Date.     

Influence of field re-ponding pattern and plant spacing on rice root–shoot characteristics,yield, and water productivity of two modern cultivars under SRI management in Indian Mollisols

A 2-year field experiment was conducted during the wet seasons (July–October) of 2008 and 2009 on a Typic Hapludoll Mollisol in Indo-Gangetic Plains Region (IGPR) to: (i) investigate the effects of field water re-ponding intervals and plant spacing on the growth, yield, and water productivity (WP) of two rice cultivars under system of rice intensification (SRI) management, and (ii) assess comparative performance of SRI versus ‘best management practices’(BMP) of rice cultivation. This experiment was designed with 14 treatments, 12 under SRI, and 2 BMP (controls). SRI treatments comprised of 3 irrigation regimes viz, irrigation at 1, 3, and 5 day(s) after disappearance of ponded water (DADPW), 2 plant spacings (20 × 20, 25 × 25 cm), and 2 rice cultivars (Pant Dhan 4 and Hybrid 6444). Two BMP (control) treatments comprised of standard cultivation recommendations for flooding and spacing. The experiment was laid-out in a factorial randomized complete block design with three replications. Statistical analysis of data revealed significant variations in root–shoot characteristics and rice yield under SRI between years, reflecting different rainfall patterns. During 2009, a low rainfall year, the panicle numbers m^?2, dry root weight m^?2, root volume m^?2, filled spikelet number panicle^?1, and filled spikelet weight panicle^?1 were significantly higher, which resulted in a rice grain yield enhancement by 5.1 % over 2008, when there was unusually heavy rainfall. Climate × irrigation regime interaction revealed a non-significant influence of irrigation regimes on growth and yield during 2008, whereas in 2009, irrigation at 1 DADPW and 3 DADPW increased grain yield by 12.8 and 8 %, respectively over 5 DADPW. Better root–zone soil moisture regimes, balancing water, and oxygen availability were responsible for higher yields under irrigation at 1 and 3 DADPW. In 2008, soil moisture content (SMC) in 0–15 cm layer was 91, 86, and 82 % of field capacity (FC) at panicle initiation, and 88, 80, and 77 % at panicle emergence stage when irrigation was at 1, 3, and 5 DADPW, respectively; the lower layers (15–30, 30–45 cm) retained their SMC between 87 and 94 % of FC at both stages. During 2009, SMC in all the three layers at both stages was more than 85 % of FC when irrigating at 1 DADPW, and a little more than 70 % for the 0–15 cm layer and >80 % for the other two layers when irrigation was done at 3 DADPW. SMC dropped to below 60 % of FC in the 0–15 cm layer and remained between 67 and 77 % of FC in the other two layers, with lower yield resulting when irrigations were applied at 5 DADPW. However, WP was the highest with irrigation at 5 DADPW (38.5 kg ha cm^?1). Wider plant spacing (25 × 25 cm) resulted in generally and significantly higher grain yield and WP. On an average, SRI (6.1 t ha^?1) resulted in yield advantage of 0.9 t ha^?1 over BMP (5.2 t ha^?1). Overall, it is inferred that in SRI, wider planting (25 × 25 cm) with field re-ponding at 3 DADPW if there is adequate water availability and at 5 DADPW under limited water supply conditions, may lead to higher rice yields and WP in sub-humid tarai Mollisols of IGPR and comparable agro-climatic conditions in Indian sub-continent.     

Influence of Iron Deficiency Anemia on Hemoglobin A1C Levels in Diabetic Individuals with Controlled Plasma Glucose Levels

Introduction: Hemoglobin A1C (HbA1c) reflects patient’s glycemic status over the previous 3 months. Previous studies have reported that iron deficiency may elevate A1C concentrations, independent of glycemia. This study is aimed to analyze the effect of iron deficiency anemia on HbA1c levels in diabetic population having plasma glucose levels in control. Methods: Totally, 120 diabetic, iron-deficient anemic individuals (70 females and 50 males) having controlled plasma glucose levels with same number of iron-sufficient non-anemic individuals were streamlined for the study. Their data of HbA1c (Bio-Rad D-10 HPLC analyzer), ferritin (cobas e411 ECLIA hormone analyzer), fasting plasma glucose (FPG, Roche Hitachi P800/917 chemistry analyzer), hemoglobin (Beckman Coulter LH780), peripheral smear examination, red cell indices, and medical history were recorded. Statistical analysis was carried out by student’s t-test, Chi-square test, and Pearson’s coefficient of regression. Results: We found elevated HbA1c (6.8 ± 1.4%) in iron-deficient individuals as compared to controls, and elevation was more in women (7.02 ± 1.58%). On further classification on the basis of FPG levels, A1C was elevated more in group having fasting glucose levels between 100-126 mg/dl (7.33 ± 1.55%) compared to the those with normal plasma glucose levels (<100 mg/dl). No significant correlation was found between HbA1c and ferritin and hemoglobin. Conclusion: This study found a positive correlation between iron deficiency anemia and increased A1C levels, especially in the controlled diabetic women and individuals having FPG between 100-126 mg/dl. Hence, before altering the treatment regimen for diabetic patient, presence of iron deficiency anemia should be considered. Key Words: Iron deficiency anemia, Hemoglobin A1C (HbA1c), Diabetes     

Mouse model of haemorrhagic septicaemia: dissemination and multiplication of Pasteurella multocida B:2 in vital organs after intranasal and subcutaneous challenge in mice

Haemorrhagic septicaemia (HS) is an endemic disease of bovines, occuring in most tropical regions of Asia and Africa. In the present study, the suitability of using mice to study pathogenesis of HS was assessed using mortality, mean death time and bacterial multiplication in vital organs after infection with live P multocida. Mice were infected with 10⁵, 10³ and 10¹?cfu of P. multocida B:2 via intranasal and subcutaneous routes along with control groups. Bacterial multiplication in lung, liver and spleen of mice were determined at 24 h interval after intranasal and subcutaneous challenge. More than 80 % of challenged mice died within 48 h of inoculation, irrespective of the dose and route of inoculation. A heavy bacterial load (up to 10⁸?cfu) was observed in lung, liver and spleen of mice titrated at 24 h and following death of mice. Results of the present study indicate that even ten bacteria are enough to cause mortality in mice and the organism multiplies rapidly in respiratory epithelium and disseminated to other vital organs viz liver and spleen suggesting the important role of mouse model in investigating the pathogenesis and challenge studies during vaccine development.     

The evaluation of endophyte toxin residues in sheep fat

Abstract

AIM: To monitor changes in concentrations of lolitrem B and epoxy-janthitrems in the fat of sheep grazing perennial ryegrass infected with wild-type- and AR37-endophyte, respectively, during the time of year when ryegrass staggers would be expected to be observed.

METHODS: Ten 5-month-old lambs with no previous exposure to endophytes were grazed on either wild-type (containing lolitrem B, n = 5) or AR37 (containing epoxy-janthitrems, n = 5) endophyte-infected perennial ryegrass (Lolium perenne L.) pastures between October 2008 and June 2009. Animals were regularly assessed for ryegrass staggers using the Keogh scale (0 = no signs, 5 = severe tremors). When a score of > 3.5 was observed animals were removed from the treatment pastures for 1 month. Fat biopsy samples were taken from each animal at approximately monthly intervals and analysed for endophyte metabolites using high performance liquid chromatography (HPLC) methods developed during this study. Regular herbage samples were also taken and concentrations of endophyte metabolites measured.

RESULTS: Efficient and reproducible methods to analyse both lolitrem B and epoxy-janthitrems in fat were developed. Concentrations of lolitrem B and epoxy-janthitrems in herbage and in sheep fat increased from late November to peak in mid-February. Ryegrass staggers was observed in both groups of sheep at this time. Following 1 month of grazing non-infected pasture mean concentrations in fat of lolitrem B decreased by 43% from 61.8 to 35.3 ppb, and of epoxy-janthitrems by 38% from 1032.0 to 639.5 ppb. Maximum concentrations in herbage of epoxy-janthitrems (35.7 ppm) were higher than of lolitrem B (3.4 ppm), but signs of staggers were less severe in sheep grazing pasture containing the former compared with the latter (median Keogh scores in late February were 2 and 3, respectively), consistent with epoxy-janthitrems being low potency toxins.

CONCLUSION: This study demonstrated that concentrations of epoxy-janthitrems and lolitrem B in sheep fat increased quickly during the initial phase of the study when concentrations in pasture increased, and decreased when animals were removed from pastures containing these compounds. These data will be used in the risk assessment of the endophyte metabolites.