Reproduction of chub, Leuciscus cephalus L., under controlled conditions

Research into artificial reproduction of chub was studied during three subsequent reproductive seasons. Chub reproduction was conducted in two different variations. In the first experiment different hormonal agents were given to fish, such as CPE, hCG, or an analogue of GnRH with dopamine antagonist (ovopel). In the second one, the optimum moment of applying of artificial reproduction was sought and the fish were only obtained an analogue of GnRH in different periods. The results obtained in two first seasons indicated that ovopel is the most effective agent for the artificial reproduction of chub. Consequently, it was decided that the fish would obtain only this agent in the final year of research. In last year (experiment II), chub spawners were taken to the hatchery under controlled conditions every few weeks from February to the beginning of June. Periodically, the maturity of oocytes was checked. As soon as the oocytes had achieved stage 2–3 of maturity, fish were obtained ovopel. It was noted that there was no problem with collecting semen. Obtaining oocytes was much more difficult. Most frequently, they were not obtained at all or obtained in very small amounts. It was also observed that the administration of hormones caused a very quick maturation of gonads and their over-maturation.     

New ELISA test for detection of bovine leukemia virus infections in cattle, using bacterially synthesized p24

A new ELISA test is described for the detection of antibodies to bovine leukemia virus protein p24. This test employs a bacterially synthesized p24 antigen which represents a hybrid protein consisting of beta-galactosidase and about 70% of the mature viral p24. The antigen preparation was enriched from Escherichia coli cells to 95% purity and was used for the detection of antibodies in cattle. In a selected set of 100 positive field sera, 97 could be verified by the new test.     

The effects of Douglas-fir on tree-specific arthropod communities in mixed species stands with European beech and Norway spruce

The ecological effects of planting exotic Douglas-fir [Pseudotsuga menziesii (Mirb.) Franco] in Central Europe are still poorly understood. The aim of this study was to answer the question of whether Douglas-fir affects tree specific arthropod communities in different mature forest types (Douglas-fir, spruce and beech dominated) in Southern Germany. Therefore, arthropod communities of stem and tree crown strata of Douglas-fir and spruce (Picea abies L.) were sampled in the years 1999–2001 using arboreal photo-eclectors and flight interception traps. Statistical analysis was conducted for all species and focused on conifer specialists at three levels: (1) species diversity, (2) guild structure and (3) community structure. Within the stem stratum, species diversity was significantly higher on spruce than on Douglas-fir independent of year and stand composition. This could not be explained by a single feeding guild, rather by species changing strata during the vegetation period. In contrast, species diversity in tree crowns was approximately the same for both conifer species. However, communities in Douglas-fir crowns were conspicuously different from those in spruce crowns, especially in the Douglas-fir dominated stand type. While zoophagous insects exhibited higher activity on Douglas-fir in 2000, xylophagous beetles were more abundant on spruce in 2001. In European beech stands with widely spaced Douglas-fir trees, the site specific and broad-leaved tree related fauna might be maintained. In addition, Douglas-fir with its resource of Adelges cooleyi and crowns that overtop the broad-leaved tree canopy, offer additional resources for several aphidophagous and thermophile species.     

The nameless counterpart: a reconstruction of the experiences of private forestry extension officers with their clients

The changes to the forest ownership structure of small private forests in recent times have opened up an intensive field of research throughout Europe. Most of the studies completed to date adhere to the classic survey model and describe the behaviour and attitudes of forest owners. The research presented in this article analyses the changes to small private forests with the aid of data collected from focus groups comprised of forestry extension officers in Germany. Through the reconstruction of the experiences gleaned by the consultants, it was possible to describe the manner in which they characterise their clientele, and the changes they have observed, against the backdrop of changes occurring across rural areas. The consultants’ strategies for describing forest owners are presented. The orientation pattern presented by the extension officers is compared with the urban orientation of forest owners’ model developed by social scientists.     

Versuche über die Empfindlichkeit vonTrichogramma (Hymenoptera,Chalcidoidea) gegenüber Fungiziden

Zusammenfassung Die Wirkung von 12 im Obstbau gebräuchlichen Fungiziden in praxisüblichen Anwendungskonzentrationen aufTrichogramma-Imagines wurde in Laborversuchen geprüft.Alle berücksichtigten Mehltau-Bekämpfungsmittel, Binapacryl, Dinocap, Triamphos und Chinomethionat, erwiesen sich als mehr oder weniger toxisch, desgleichen die Schorf-Bekämpfungsmittel Mancozeb, Dichlofluanid, Metiram und TMTD. Für die Schorf-Bekämpfungsmittel Captan, Dithianon, Dodin und Zineb wurde unter den Versuchsbedingungen keine Giftwirkung nachgewiesen.Die Angaben der Literatur über die Wirkung der genannten Fungizide aufTrichogramma und andere Chalcidoidea werden diskutiert.

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Summary The effects uponTrichogramma adults of 12 fungicides which are commonly in use in orchards, in normal concentrations were examined by laboratory trials.The mildew fungicides, binapacryl, dinocap, triamphos, and oxythioquinox proved to be toxic as well as the apple scab fungicides mancozeb (= coordination product of zinc ion and [ethylenebis(dithiocarbamato)] manganese), dichlofluanid, metiram (= zinc-activated polyethylene thiuram disulfide), and thiram. Under the conditions of the experiments, there was no evidence of a toxic effect exerted by the apple scab fungicides captan, dithianon, dodine, and zineb.The available data from literature on the effects of the fungicides mentioned uponTrichogramma and other chalcidoids are discussed.

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Durchgeführt mit Unterstützung der Deutschen Forschungsgemeinschaft.

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Dem Direktor des Instituts, Herrn Professor Dr. J. M. Franz, danke ich für sein reges Interesse an den Versuchen und die mir jederzeit gewährte Unterstützung.     

Homogeneous shear stress field of wood in an Arcan shear test configuration measured by means of electronic speckle pattern interferometry: description of the test setup

Wood Science and Technology - Several studies have dealt with the problem of how to measure the shear modulus of small clear wood specimens, avoiding bias from normal compression, bending, tension...     

Differential Activity of Carboxylic Acid Amide Fungicides Against Various Developmental Stages of Phytophthora infestans

ABSTRACT Three carboxylic acid amide (CAA) fungicides, mandipropamid (MPD), dimethomorph (DMM) and iprovalicarb (IPRO) were examined for their effects on various asexual developmental stages of Phytophthora infestans in vitro and in planta. Germination of cystospores and direct germination of sporangia were inhibited with nanomole concentrations of MPD (0.005 mug/ml) and micromole concentrations of DMM (0.05 mug/ml) or IPRO (0.5 mug/ml). A temporary exposure of 1 h to CAAs was not detrimental to germination and infectivity of sporangia or cystospores. CAAs applied to cystospores at 1 h after the onset of germination did not prevent the emergence of germ tubes, but inhibited their further growth and deformed their shape. None of the fungicides affected discharge of zoospores from sporangia or the encystment (cell wall formation/assembly) of the zoospores. Mycelium growth in solid or liquid media was inhibited with micromole concentrations. CAAs mixed with sporangia and drop inoculated onto detached leaves strongly suppressed infection. Curative application at 1 day postinoculation (dpi) required higher concentrations of CAAs than preventive application to inhibit infection and lost its effectiveness at 2 dpi. When sprayed on established late blight lesions 4 days after inoculation, CAAs reduced sporangial production in a dose-dependent manner. Trans-laminar protection of potato or tomato leaves, although achieved with higher doses, was more effective with MPD than with DMM or IPRO. Shade house studies demonstrated superior control of late blight epidemics by MPD compared with the other molecules. The data suggest that germ tube formation by cystospores or sporangia is the most sensitive stage in the life cycle of P. infestans to CAAs. Of the three CAAs, MPD had the highest intrinsic activity against spore germination. This property, together with its better trans-laminar activity, makes MPD more effective than DMM or IPRO in controlling epidemics caused by P. infestans.