全文获取类型
收费全文 | 15377篇 |
免费 | 958篇 |
国内免费 | 1460篇 |
专业分类
林业 | 1013篇 |
农学 | 663篇 |
基础科学 | 700篇 |
1642篇 | |
综合类 | 7729篇 |
农作物 | 1062篇 |
水产渔业 | 704篇 |
畜牧兽医 | 2454篇 |
园艺 | 1228篇 |
植物保护 | 600篇 |
出版年
2024年 | 80篇 |
2023年 | 282篇 |
2022年 | 587篇 |
2021年 | 624篇 |
2020年 | 571篇 |
2019年 | 541篇 |
2018年 | 380篇 |
2017年 | 710篇 |
2016年 | 426篇 |
2015年 | 782篇 |
2014年 | 791篇 |
2013年 | 953篇 |
2012年 | 1411篇 |
2011年 | 1452篇 |
2010年 | 1387篇 |
2009年 | 1241篇 |
2008年 | 1242篇 |
2007年 | 1083篇 |
2006年 | 888篇 |
2005年 | 685篇 |
2004年 | 425篇 |
2003年 | 276篇 |
2002年 | 297篇 |
2001年 | 259篇 |
2000年 | 221篇 |
1999年 | 93篇 |
1998年 | 10篇 |
1997年 | 11篇 |
1996年 | 13篇 |
1995年 | 8篇 |
1994年 | 13篇 |
1993年 | 12篇 |
1992年 | 6篇 |
1991年 | 5篇 |
1990年 | 8篇 |
1988年 | 1篇 |
1987年 | 2篇 |
1986年 | 4篇 |
1981年 | 4篇 |
1980年 | 1篇 |
1978年 | 1篇 |
1962年 | 1篇 |
1959年 | 1篇 |
1958年 | 1篇 |
1957年 | 1篇 |
1956年 | 2篇 |
1955年 | 3篇 |
排序方式: 共有10000条查询结果,搜索用时 296 毫秒
31.
AIM:To investigate the effect of the heat shock response on the reperfusion arrhythmias(RAs) and the possible mechanism involved. METHODS:Fifty-five Sprague Dawley rats were randomly divided into 2 groups: the heat shock group (group H,n=29) and the control group (group C,n=26). The rats in group H were preconditioned with heat shock 24 hours before, and that in group C were not. The hearts of 16 rats in group H and 16 in group C were exercised and mounted on a non-circulating Langendorff perfusion apparatus and perfused retrogradely with modified K-H buffer and mimic ischemia/reperfusion was applied. Additionally, conventional intracellular microelectrode techniques were used for recording such electrophysiological parameters as resting potential(RP), action potential amplitude(APA), over shot(OS), maximum depolarization velocity(Vmax) of the hearts of other 13 rats in group H and 10 in group C. RESULTS:①Prior heat stress significantly decreased reperfusion arrhythmia. ②The amount of CK release in the effluent in group H was much less than that in group C. ③Myocardial HSP70 content was elevated significantly in group H. ④Heat stress significantly increased myocardial anti-oxydases activity and decreased lipid peroxydative products. Additionally, heat stress significantly reduced the Vmax of action potential. It indicated that rapid Na+ channel of papillary muscles may be inhibited by heat shock. The degree of change of Vmax after ischemia in H group was significantly less than that in group C. And the time of reperfusoin with Tyrode's solution till the action potential appeared as large as that before perfusion with mimic ischemic solution is shorter in group H than in group C. CONCLUSION:Heat shock pretreatment markedly reduces ischemia/reperfusion-induced injury of heart and ventricular arrhythmias in rats and this effect may be associated with the inhibition of rapid Na+ channel of papillary muscles by heat shock and the increase in myocardial HSP70 and anti-oxydase activity. 相似文献
32.
AIM:To examine the effects of recombinant human hepatocyte growth factor(rhHGF) and native calf HGF(cHGF) on SMMC-7721 human hepatocellular carcinoma(HCC) cell line. METHODS:Human HCC cell line culture, photometric assay, and flow cytometric assay were used in this study .RESULTS:A similar type of dose-dependent cell growth inhibition effect on SMMC-7721 human HCC cells by rhHGF(5-20 μg/L) as well as by cHGF(25-100 mg/L) had been found, with the maximal effect at the highest concentration used. Approximately over 50% of the cells treated with rhHGF(5 μg/L, 10 μg/L, 20 μg/L) accumulated in the quiescent G0/G1 phase of the cell cycle over incubation periods for 3 d. CONCLUSION:The growth of SMMC-7721 human HCC cells was strongly inhibited by both rhHGF and cHGF. This might be because the cells exposed to HGF became arrested in the G0/G1 phase. 相似文献
33.
AIM: To observe the effect of Jiawei Sini Decoction (JWSND) on glucocorticoid receptor (GCR) in thymocytes of chronic psychological stress rats. METHODS: The rats were randomly divided into 4 groups, control group (C), model group (M), group treated by JWSND C1, group treated by ginsenosides C2. The number of thymocyte GCR sites and the GCR nuclear thanslocation rate were detected by radioimmunoassay. RESULTS: Compared with the control group, in the model group, the thymocyte weight index lowered significantly ( P<0.05 ), and the GCR nuclear thanslocation rate was increased significantly ( P<0.01 ), but the number of thymocyte GCR sites was unchanged. Compared with the model group, thymus gland weight indexes of C1 and C2 were increased significantly ( P<0.05 ), while the GCR nuclear thanslocation rate lowered significantly ( P<0.01 ). Moreover, no significant difference was found in all indexes between C1 and control group. CONCLUSION: The inhibitory effect of glucocorticoid on the thymus could be significantly reversed by JWSND via suppressing the thanslocation of GCR from cytoplasm to nucleus in chronic psychological stress rats. 相似文献
34.
The abnormality of the central noradrenergic system in Alzheimer's disease has two cases: decrease or increase. The former is easy to be understood, because it is resulted from the outstanding forfeit of the noradrenergic neurons in the nucleus locus ceruleus (nLC); but the noradrenalin concentration does not change and enen increase, it seems to disagree with the loss of the noradrenergic neurons in the nLC. This article put emphasis on it, enumerated the related evidence and analyzed the possible causes. At present time, the drugs to increase noradrenalin is used mostly in clinic. This paper summarized it and put forth our own opinions. 相似文献
35.
CHEN Xiao-yin ZHANG Qun YANG Qin-he XIE Qiu-ling SHEN Qiang XU Yun-sheng 《园艺学报》2002,18(12):1529-1531
AIM: To examine the effect of traditional chinese medicine recipe, Taoren Honghua(semen persicae-flos carthami) decoction, on hyperlipidemia without symptom. METHODS: The plasma TC, TG, LDL, HDL, apolipoprotein(Apo) A, Apo Bof the patients with hyperlipidemia without symptom were measured using automatic analyzer (shimadiu CL-7200), the production of nitric oxide(NO) was detected by Greiss reaction, and SODactivity and MDAformation were examined using o-trihydroxy benzene and barbituric methotheds, respectively. RESULTS: After oral administration of Taoren Honghua decoction, the plasma levels of TC, TG, LDL, and MDAof the patients were markedly decreased, however, the plasma levels of ApoA, HDL, SODand NOwere significantly increased and almost no change was detected in the plasma level of Apo B. In control group, it was found that although the plasma level of TC, TG and LDL were decreased ( P<0.05 ) and ApoAas well as HDLwere increased, ApoB, SOD, MDA and NO production were all unchanged. CONCLUSION: The traditional chinese medicine recipe, Taoren Honghua decoction,has a significant therapeutic effect on patients with hyperlipidemia by removing blood stasis, promoting qi circulation and in turn reducing blood lipid level. 相似文献
36.
37.
38.
39.
从姬松茸子实体的热水提取物中分离纯化出组分ABP-Ⅱ-a。高压液相层析测得其相对分子量为52500,红外扫描显示有典型的多糖吸收峰。气相色谱分析表明,其单糖组成为葡萄糖、甘露糖、半乳糖和少量阿拉伯糖,摩尔比为35.24:5.19:1.91:1。 相似文献
40.