Effects of Annealing and Concentration of Calcium Salts on Thermal and Rheological Properties of Maize Starch During an Ecological Nixtamalization Process

The objective of the present work was to study the effect of annealing and concentration of Ca(OH)₂ (lime) and calcium salts on the thermal and rheological properties of maize starch during an ecological nixtamalization process. Thermal and rheological properties of maize starch changed during the ecological nixtamalization process because of three main causes: the annealing phenomenon, type of calcium salt, and calcium salt concentration. In all treatments thermal properties (T_o, T_p, and T_f) of nixtamal starch increased owing to the annealing process, whereas the type of salt or lime increased thermal properties and decreased pasting properties in this order: CaCl₂ > CaSO₄ > Ca(OH)₂ ≈ CaCO₃. This behavior was because of the dissociation of each salt or lime in water. Anions (OH⁻) can penetrate much more easily into the starch granule and start the gelatinization process by rupturing hydrogen bonds. Additionally, amylose‐lipid complexes were formed during the nixtamalization processes, as indicated by an increasing peak at 4.5 Å in X‐ray diffraction patterns.     

TECHNIQUE,DIFFICULTY, AND ACCURACY OF COMPUTED TOMOGRAPHY‐GUIDED TRANSLAMINAR AND TRANSFORAMINAL LUMBOSACRAL EPIDURAL AND INTRAARTICULAR LUMBAR FACET JOINT INJECTIONS IN DOGS

In human medicine, spinal pain and radiculopathy are commonly managed by computed tomography (CT)‐guided facet joint injections and by transforaminal or translaminar epidural injections. In dogs, CT‐guided lumbosacral epidural or lumbar facet joint injections have not been described. The aim of this experimental, ex vivo, feasibility study was to develop techniques and to assess their difficulty and accuracy. Two canine cadavers were used to establish the techniques and eight cadavers to assess difficulty and accuracy. Contrast medium was injected and a CT scan was performed after each injection. Accuracy was assessed according to epidural or joint space contrast opacification. Difficulty was classified as easy, moderately difficult, or difficult, based on the number of CT scans needed to guide insertion of the needle. A total of six translaminar and five transforaminal epidural and 53 joint injections were performed. Translaminar injections had a high success rate (100%), were highly accurate (75%), and easy to perform (100%). Transforaminal injections had an moderately high success rate (75%), were accurate (75%), and moderately difficult to perform (100%). Success rate of facet joint injections was 62% and was higher for larger facet joints, such as L7‐S1. Accuracy of facet joint injections ranged from accurate (37–62%) to highly accurate (25%) depending on the volume injected. In 77% of cases, injections were moderately difficult to perform. Possible complications of epidural and facet joint injections were subarachnoid and vertebral venous plexus puncture and periarticular spread, respectively. Further studies are suggested to evaluate in vivo feasibility and safety of these techniques.     

A SURVEY OF RADIOLOGISTS AND REFERRING VETERINARIANS REGARDING IMAGING REPORTS

An imaging report is a vital communication tool between a radiologist and clinician. In a field where in‐person communication may not be readily available, it is imperative that the report clearly relays pertinent clinical information in a timely manner. The purpose of this observational study was to describe and compare opinions and expectations of small animal general practitioners, veterinary specialists, and veterinary radiologists regarding the imaging report. Online surveys were distributed, and data were collected from 202 veterinary clinicians and 123 veterinary radiologists. The majority (89%) of clinicians were satisfied with their imaging reports and stated that they read the radiology report as soon as it was available (92%). Just less than half (48%) of clinicians indicated it was standard of care that a board‐certified veterinary radiologist read all imaging studies. Radiologists and clinicians agreed that a clinical history (98% and 94%, respectively) and clinical question (82% and 68%, respectively) were needed to generate a good radiology report. Fifty‐five percent to 70% of clinicians prefer red bulleted reports, which included incidental findings (96%); while radiologists slightly favored prose reporting (37–46%). Clinicians found it helpful when additional imaging (86%), medical (71%), and surgical recommendations (73%) were made. About one‐third of specialists who had been in practice for >11 years thought they were better able to interpret imaging for their own specialty than the radiologist. Clinicians voiced discontentment with reports that were not completed in a timely manner or did not give a prioritized differential list. Further studies are warranted to provide a more in‐depth evaluation of veterinary radiology reporting structure and style.     

Genetic monitoring of ex situ African Penguin (Spheniscus demersus) populations in South Africa

The African Penguin (Spheniscus demersus) has suffered population declines and is listed in the IUCN Red List as Endangered. The species is endemic to the coast of southern Africa, and breeding colonies are distributed on the south-western coast of Africa. Currently, African Penguins are being kept in zoo and aquarium facilities throughout South Africa. In this study, molecular genetic data based on 12 microsatellite markers from 1 119 African Penguin samples from four facilities were generated in order to determine the level of genetic variation, population structure and differentiation, and effective population size to assist in the development of an effective captive management plan. Expected heterozygosity ranged from 0.57 to 0.62, and allelic richness from 4.2 to 5.1. However, based on differences between first- and second-generation captive birds, we conclude that the ex situ population is at risk of losing genetic variability in the future and management programmes should include exchange of birds between captive facilities in order to induce gene flow and increase effective population size. Adding individuals from in situ populations should also be considered in the future in cases where these birds cannot be rehabilitated. Molecular genetic analyses of wild penguin populations should be carried out for comparison, and to ascertain to what degree ‘in situ genetic diversity’ is represented among ex situ populations. With regular resampling and analyses, the extent of the effect of processes such as genetic drift on diversity in the ex situ penguin populations will become evident.     

A new galago species for South Africa (Primates: Strepsirhini: Galagidae)

The primate fauna of South Africa has historically been viewed as comprising three diurnal cercopithecoid taxa – chacma baboons (Papio ursinus), vervet (Chlorocebus pygerythrus) and samango monkeys (Cercopithecus albogularis) – and two nocturnal lorisoid species – the thick-tailed greater galago (Otolemur crassicaudatus) and the southern lesser galago (Galago moholi). Here we report the positive identification of a third galago species within South Africa’s borders: the Mozambique dwarf galago or Grant’s galago, Galagoides granti (Thomas and Wroughton, 1907). The taxon was previously held to be restricted to Mozambique, eastern Zimbabwe, Malawi and Tanzania, but we have also observed it in the sand forest of Tembe Elephant Park and the Tshanini Community Reserve, near the Mozambique border. The species was formerly mistaken for Galago moholi, erroneously (we believe) extending the range of the latter species into northern KwaZulu-Natal. In South Africa the two small galagos are unlikely to have overlapping ranges: Galago moholi prefers dry savanna woodlands, whereas Galagoides granti is apparently confined to dry sand forest. However, both species may coexist with the larger and more widespread Otolemur crassicaudatus, an inhabitant of moist savanna, forest edge and thicket. The true South African ranges of both small galago species need to be ascertained.     

Skull size and shape variation in Psammomys spp. (Rodentia,Gerbillinae) from Tunisia,with emphasis on the impact of allometric variation on species recognition

In Tunisia both Psammomys obesus and P. vexillaris are found. These taxa have been the subject of taxonomic controversy for some time, due to variability in the classical morphological characters used for taxonomic recognition. In this study we investigated skull size and shape variation in the genus Psammomys by using geometric morphometrics to evaluate the extent of intra- and interspecific shape variation and explicitly tested for the impact of allometric shape variation on species discrimination. Eleven populations of the two species from 10 localities in Tunisia were studied. Statistical analyses of size and shape showed large size variation within P. obesus, but no shape differences were revealed among populations of this species. Interspecific analysis revealed that P. vexillaris had the smallest skull. Principal component analysis and Procrustes distances showed good discrimination between the two species after removal of the allometric component of shape variation. The results obtained show that allometric-related shape variation could mask discrimination between the two Psammomys species. This finding might explain the uncertainty in classification of these species in the past. The interspecific allometric-free phenotypic differences observed may be associated with adaptive processes linked to the different environmental and trophic preferences of the two species.