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选用来源于中国黄淮和美国的熟期组II~IV的8个大豆品种, 按Griffing方法II设计, 配成28个双列杂交组合, 包括8个亲本共计36份材料。选用300个SSR标记, 对8个大豆亲本进行全基因组扫描, 利用基于回归的单标记分析法, 对大豆杂种产量和分子标记进行相关性分析, 估计等位变异的效应和位点的基因型值, 剖析杂种组合的等位变异。结果表明, 300个SSR标记中有38个与杂种产量显著相关, 分布于17个连锁群上, 其中D1a和M等连锁群上较多, 有8个位于连锁定位的QTL区段内(±5 cM)。单个位点可分别解释杂种产量表型变异的11.95%~30.20%。杂种的位点构成中包括有增效显性杂合位点、增效加性纯合位点、减效加性纯合位点和减效显性杂合位点4部分, 其相对重要性依次递减。从38个显著相关的SSR标记位点中, 遴选出Satt449、Satt233和Satt631等9个优异标记基因位点, Satt449~A311、Satt233~A217和Satt631~A152等9个优异等位变异, 以及Satt449~A291/311、Satt233~A202/207和Satt631~A152/180等9个优异杂合基因型位点。这些结果为理解杂种优势的遗传构成和大豆杂种产量聚合育种提供了依据。 相似文献
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Soybean cyst nematode (SCN Heterodera glycines Ichinohe) is one of the most important nationwide soybean diseases in China. A total of 38 soil specimens or locations in the area was sampled and tested for SCN races during 2001-2003 for the inspection of race distribution in Huang-Huai Valleys. A map of race distribution was constructed according to the data from both the present study and the published reports cited. Three areas, namely, the area of southeast to Jinan in Shangdong Province; the area of northern Henan Province and its border region to south of Hebei Province; and the area of Luohe, Zhoukou of Henan Province and Fuyang of Anhui Province mainly infested with Race 1 were identified. Race 4 was predominant in Shanxi Province, Beijing and the adjacent area of Henan, Shandong, and Anhui provinces, and the delta of Huanghe River in Shandong Province. Race 2 was mainly found in Liaocheng, Dezhou of Shangdong Province and Shijiazhuang of Hebei Province, and Jiaozuo and Huojia of Henan Province. Race 7 was distributed in the west part of Jiaodong Peninsula of Shandong Province and Kaifeng, Huaxian, Wenxian of Henan Province. Race 5 was found and scattered in Hebei and Henan Province. Race 9 was found in Shangqiu of Henan Province, which was reported for the first time in China. It can be seen that Race 1 and Race 4 were the two predominant races in Huang-Huai Valleys, and that research should focus on developing resistant cultivars of these races. There might exist other races in an area with some predominant races. The race substitution in the past decade was not obviously found, therefore, the results should be meaningful to future breeding for resistance to SCN in Huang-Huai Valleys. 相似文献
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大豆对SMV数量抗性的表现形式与种质鉴定 总被引:7,自引:1,他引:7
在发现大豆对SMV具有数量抗性的基础上,选用96份大豆品种材料研究其在接种Sa、SC8、N1、N3等4个SMV株系条件下的发病率、病级、潜育期和病害扩展速度等4个数量抗性组分的变异。结果表明,品种间4个组分均存在明显差异;溧水中子黄豆、沛县天鹅蛋、诱变30等品种虽然对4个株系均感染,但在4个组分上均表现出较强抗性,且不同株系间差异较小,说明这些品种对大豆花叶病毒具有广谱数量抗性。研究证实以往报道的一些抗感染品种如溧水中子黄豆、AGS-19,其实是抗扩展的数量抗性品种。邳县茶豆、淮阴秋黑豆等品种对SMV的抗性可能属于数量抗性与质量抗性的叠和。大豆对SMV的数量抗性是曾被学术界忽视而又值得利用的一类抗性,它一般比质量抗性具有更宽的抗谱和更好的持久性。 相似文献
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Performances and Germplasm Evaluation of Quantitative Resistance to Soybean Mosaic Virus in Soybeans
A sample composed of 96 soybean accessions was evaluated for their diseased rate (I),diseased rank (S), latent period (LP) and rate of disease development (R) in order tostudy the quantitative resistance to soybean mosaic virus (SMV) in soybeans. The resultsshowed that the performances of the above four resistance components were significantlydifferent among accessions and that some of the accessions, such as Zhongzihuangdou,Peixian Tianedan, Youbian30 could be infected by four SMV strains, Sa, SC8, N1 and N3,but their I, S, and R were lower and LP longer than most other accessions. These resultsdemonstrated the existence of quantitative resistance to SMV in soybeans. It was foundthat some soybean accessions, such as AGS19 and Lishui Zhongzihuangdou, previouslyidentified as resistant to SMV infection, performed some infection but resistant toexpansion in the present study. In addition, the resistance in Pixian Chadou and HuaiyinQiuheidou might be either qualitative or quantitative. Furthermore, the present studyalso indicated that the resistance spectrum and durability of accessions with quantitativeresistance might be wider and longer than those with qualitative resistance. 相似文献