MD病毒抗独特型抗体的研制

制备鸡马立克氏病病毒(MDV)抗独特型抗体(Ab2),探讨Ab2模拟抗原诱导免疫应答的能力.用MDV免疫SPF鸡,分离提纯鸡抗MD-IgG(Ab1),Ab1免疫BALB/C鼠,用鼠脾细胞与SP2/0细胞制备Ab2杂交瘤细胞株,间接ELISA筛选阳性杂交瘤细胞株,经克隆、纯化2×株抗MDV独特型抗体杂交瘤细胞株(6E5,7A12),其中6E5培养上清、腹水ELISA效价分别为4000×、10000×,免疫20 d攻毒,具有抗MDV感染保护.     

建筑物的机械排烟

本文作者从实际经验出发,详细阐述了建筑物的机械排烟原理及设计施工,管理中的各项注意事项。     

从证券资本市场拓宽湖南林业产业化发展空间

发展证券资本市场可有效地推动经济快速发展 ,但林业产业上市融资明显处于低水平状态。湖南省林业产业化发展 ,客观上要求在证券资本市场上拓宽发展空间 ,为此提出林业产业发展和上市融资需采取的 6项对策与措施。     

The immunohistochemical detection of Mycoplasma bovis and bovine viral diarrhea virus in tissues of feedlot cattle with chronic, unresponsive respiratory disease and/or arthritis.

The purpose of this study was to determine the frequency of selected pathogens in the tissues of a group of feedlot cattle with chronic disease (most often respiratory disease and/or arthritis). Samples of lung and joint tissues from 49 feedlot animals that had failed to respond to antibiotic therapy were tested by immunohistochemical staining for the antigens of Mycoplasma bovis, Haemophilus somnus, Pasteurella (Mannheimia) hemolytica, and bovine viral diarrhea virus (BVDV). Mycoplasma bovis was demonstrated in over 80% of cases, including in 45% of joints and 71% of lungs tested. Mycoplasma bovis was the only bacterial pathogen identified in the joints. Haemophilus somnus and Pasteurella (Mannheimia) haemolytica were found in 14% and 23% of cases, respectively, and were confined to the lungs in all instances. Infection with BVDV was demonstrated in over 40% of cases. Mycoplasma bovis and BVDV were the most common pathogens persisting in the tissues of these animals that had failed to respond to antibiotic therapy.     

关于我国海水网箱养鱼发展现状与危机分析及可持续发展的探讨

我国海水网箱养鱼发展现状是以个体联合体生产经营为主体的低技术水平的数量规模化，众多传统港湾已形成了大范围的严重超负荷养殖状态，其对环境巨大的污染力引发鱼病猖獗和频受赤潮侵袭，已面临严峻的养殖环境危机。本文综合分析了我国海水网箱养鱼的发展现状与负面效应及其现状危机，并初步进行了危机根源和可持续发展的讨论分析。     

Enhancement of sulfide auto-oxidation by metal ion in artificial seawater and a seawater which is a possible source of aoshio

SUMMARY: Aoshio is hypoxic milky blue-green seawater observed in some eutrophic bays. Previous studies have shown that colloidal sulfur causes the coloration and that the source of aoshio water is attributed to coastal anoxic bottom water. Occurrences of aoshio have been reported in limited areas of coastal seawater, although hypoxic transparent water seems rather universal. Promotion in auto-oxidation of sulfide by metal ions in seawater was investigated to explain the occurrences of aoshio. Artificial seawater containing 10 μM metal ion was assayed for the sulfide oxidation rate. The velocity constant which represents the oxidation rate within the initial 30 min and the amount of reacted sulfide in 2 h were determined by oxygen monitoring and sulfide quantification, respectively. Fe²⁺ and Cu²⁺ enhanced the initial 30 min reaction. Fe²⁺, Fe³⁺ and Ni²⁺ increased the amount of reacted sulfide in 2 h, forming whitish turbid water. Seawater from a suspected source of aoshio water was also assayed for the auto-oxidation rate of sulfide. The oxidation rate in water from 12 m depth was 13–19 times higher than the artificial seawater without an addition of heavy metal ions. More than 15% of the oxidation rate in 12 m deep seawater was explained by dissolved iron in the seawater.     

对我国节水灌溉技术发展的几点思考

我国节水灌溉技术的推广应用多年来在国家有关部门的领导下 ,通过广大科技、生产和应用人员的共同努力 ,取得了显著成效。面对新世纪的来临和我国加入世贸组织 ,我国节水灌溉技术如何发展 ,以适应新时期新形势的要求 ,笔者浅见如下。1　进一步提高推广节水灌溉技术对我国经济持续发展重要意义的认识　　我国是一个水资源相对短缺的国家 ,水资源总量约为 2 .8万亿ｍ3,人均占有量仅 2 2 0 0ｍ3,不足世界人均占有量的 1 4,平均每公顷占有量仅 12 0 0 0ｍ3,只有世界平均水平的 1 2。进入 90年代以来 ,我国农业年均受旱面积达2 0 0 0万ｈｍ2 以上…     

采种大白菜地膜复盖的研究

为了提高大白菜(B. campestris ssp pekinensis.)种子的产量和质量,我们进行了两年地膜覆盖研究。结果表明:无论直播小株、育苗小株、老株那种采种方式,凡使用地膜覆盖的均比不覆盖的产量增加30％左右,质量也明显的提高;聚乙烯、聚氯乙烯都是良好的地而覆盖材料,在大白菜种子生产中可以取得相近似的效果。     

延边地区紫苏保护技术的研究

紫苏是延边地区的重要经济作物之一．但目前随着种植年限的延续，开始大量发生虫害、病害以及草害等，从而导致减产、降效，严重影响苏子生产．研究结果表明，延边紫苏地适宜土壤处理的除草剂为敌草胺与扑草净的混剂，使用剂量为3kg／hm^2 5kg／hm^2，一年生杂草除草效果可达到90％以上；茎叶处理除草剂为拿捕净(12．5％，1．0kg／hm^2)、稳杀得(35％，1．0kg／hm^2)、精禾草克(5％，1．0kg／hm^2)；紫苏的主要病害为锈病，对紫苏叶片质量影响较大；主要食粒虫害(待定)对产量的影响较大．