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101.
García-Alonso B Peña-Egido MJ García-Moreno C 《Journal of agricultural and food chemistry》2001,49(1):423-429
A treatment with cyanide for the analysis of S-sulfonates in meat and meat derivatives, after a study of the effectiveness of this agent and that of dithiothreitol (DTT), is proposed. Once the protein-bound sulfite has been released, it is determined by HPLC ion exclusion with electrochemical detection. In the assay on the reproducibility of the method, standard deviations were 7.4, 9.2, and 11.4 for mean S-sulfonate values of 69, 107, and 130 microg of SO(2)/g, respectively. Mean recovery was 91.2% for different amounts (56, 111, and 223 microg of SO(2)/g) of S-sulfocysteine added. A study was made of the formation of S-sulfonates in model systems and in meat from different species-chicken and beef-with different fat contents. In the assays with meat, two different levels of sulfite addition were used: 600 and 1200 microg of SO(2)/g. From the assays carried out in model systems with sulfite and cystine it may be concluded that one factor limiting the interaction is the accessibility to disulfide groups. The proportion of S-sulfonates in sulfited meat remains relatively constant and does not seem to be governed by the meat component, the level of sulfite addition, or the fat content. However, the latter two factors are inversely correlated with the retention of sulfite in the foods analyzed. 相似文献
102.
Grinding is a critical step in the preparation of espresso coffee (EC). The addition of sugar during the torrefacto roasting process could influence the degree of brittleness and grinding. The aim of this work was to study the influence of the grinding grades (coarse, fine, and very fine) in Arabica/Robusta 20:80, natural roasted (A20:R80), and Arabica/Robusta 20:80 with 50% Robusta torrefacto roasted (A20:R80 50% torrefacto) on the chemical and sensorial characteristics of EC in order to select the optimal espresso grinding grade. A higher percentage of coarse particles was found in A20:R80 ground coffee. In both ECs, the extraction of solids and soluble and aroma compounds increased inversely with particle size. Higher foam indices and extraction yields were found in A20:R80 50% torrefacto ECs probably due to the solubilization of caramelized sugar and melanoidins. It has been suggested that the range of an acceptable extraction yield could be extended to 25% in A20:R80 50% torrefacto ECs. In conclusion, the optimal grinding grade for the obtainment of an EC with A20:R80 was fine and that for A20:R80 50% torrefacto was coarse. 相似文献
103.
Gamboa-Angulo MM Escalante-Erosa F García-Sosa K Alejos-González F Delgado-Lamas G Peña-Rodríguez LM 《Journal of agricultural and food chemistry》2002,50(5):1053-1058
Two novel phytotoxins, 8-zinniol methyl ether (5) and 8-zinniol acetate (6), in addition to 6-(3',3'-dimethylallyloxy)-4-methoxy-5-methylphthalide (2), 5-(3',3'-dimethylallyloxy)-7-methoxy-6-methylphthalide (3), and the novel metabolites 8-zinniol 2-(phenyl)ethyl ether (4) and 7-zinniol acetate (7) have been identified as natural zinniol derivatives from the organic crude extract of Alternaria tagetica culture filtrates. Using zinniol as the starting material, phytotoxin 5 was synthesized, together with a number of synthetic intermediates (8-13). Both natural and synthetic zinniol derivatives were evaluated in the leaf-spot bioassay against marigold leaves (Tagetes erecta). 相似文献
104.
Andueza S Maeztu L Dean B de Peña MP Bello J Cid C 《Journal of agricultural and food chemistry》2002,50(25):7426-7431
Water pressure is one of the most important factors which influence the final quality of espresso coffee (EC). However, few studies dealing with this issue have been found. The aim of this work was to study the effect of water pressure on the final quality of Arabica ECs as well as to classify ECs prepared at different pressures (7, 9, and 11 atm) according to their physicochemical and sensory characteristics, key odorants, by means of multivariate analysis. Statistically, principal component 1 (PC1) separated ECs prepared at 7 and 9 atm from ECs prepared at 11 atm and included the main foam and taste characteristics as well as some key odorants and flavor compounds. ECs prepared at 7 and 9 atm were separated by principal component 2 (PC2). Coffees prepared at 9 atm showed consistency of foam and a high percentage of key odorants related to freshness and fruity, malty, and buttery flavors. A simple discriminate function was obtained by discriminate analysis, allowing the classification of ECs prepared at three pressures into their respective groups with a success rate of 100%. 相似文献
105.
J. Lage B. Skovmand R. J. Peña S. B. Andersen 《Genetic Resources and Crop Evolution》2006,53(5):955-962
The wild diploid goat grass (Aegilops tauschii Cosson), and the cultivated tetraploid emmer wheat (Triticum turgidum L. subsp. dicoccon (Schrank) Thell.) may be important sources of genetic diversity for improving hexaploid bread wheat (Triticum aestivum L.). Through interspecific hybridization of emmer wheat and Ae. tauschii, followed by chromosome doubling, it is possible to produce homozygous synthetic hexaploid wheat. Fifty-eight such synthetic
hexaploids were evaluated for grain quality parameters: grain weight, length, and plumpness, grain hardness, total protein
content, and protein quality (SDS-Sedimentation volume, SDS-S). Most synthetics showed semi-hard to hard grain texture. Results
showed significant genetic variation among the synthetic hexaploids for protein content, SDS-S values, and grain weight and
plumpness. Quality measurement values of synthetic hexaploids were regressed on corresponding values of the emmer wheat parents.
With this offspring-parent regression, protein content and SDS-S values explained 8.7 and 28.8%, respectively, of the variation
among synthetics, indicating a significant contribution from the emmer wheat parents for these traits. The synthetic hexaploids,
in general, had significantly higher protein content (15.5%, on average) and longer grains than ‘Seri M82’, the bread wheat
control (13.1% protein content). Synthetics with SDS-S values and grain weights higher than those of ‘Seri M82’ were also
identified. Protein content among synthetics showed significantly negative correlations with grain weight and plumpness, but
no correlation with SDS-S values. Despite these negative correlations, 10 superior synthetic hexaploid wheats, derived from
nine different emmer wheat parents and with above average levels of protein content, SDS-S values, and either grain weight
or plumpness, were identified. This study shows that genetic variation for quality in tetraploid emmer wheat can be transferred
to synthetic hexaploid wheats and combined with plump grains and high grain weight, to be used for bread wheat breeding. 相似文献
106.
R E Calaf J Pe?a S Paytubi M Carrascal M Posada E Gelpi J Abian 《Journal of agricultural and food chemistry》2001,49(11):5085-5091
Fatty acid esters of 3-(N-phenylamino)-1,2-propanediol are currently considered the best chemical markers of toxic oils related to the Spanish toxic oil syndrome. Recent research in this area has undertaken the exhaustive and quantitative characterization of these compounds in oils collected during the epidemic outbreak. Current methods developed in this laboratory are based on solid phase extraction (SPE) using SCX cartridges followed by HPLC-APCI/MS/MS quantification. To circumvent the long and tedious extraction procedure, the SPE protocol was adapted for automatic extraction and the problems derived from the use of the immiscible solvents required for the SCX extraction were solved. Linearity of the analytical method was found in the same range as for the manual method. Extraction recoveries were 87 and 75% for 2-hydroxy-3-(N-phenylamino)propyl linoleate and 2-(linoleyloxy)-3-(N-phenylamino)propyl linoleate, respectively, and the corresponding coefficients of variation were approximately 1%, greatly improving reproducibility over manual procedures. 相似文献
107.
Terpenes and C(13) norisoprenoids are among the most important aromatic compounds found in a volatile and nonvolatile form in grapes. Aromatic typicity of a spirit could be attributed to these compounds despite the very important presence of volatile compounds produced during alcoholic fermentation. In this study, following a solid phase extraction stage, the determination of the varietal aromatic compounds by gas chromatography was performed on 15 samples of Galician orujo spirits. The results show that significant differences (p < 0.05) exist in the concentrations of varietal aromatic compounds in Galician spirits obtained from different varieties of grapes. alpha-Ionona is the varietal aromatic compound that is most likely to contribute to the aroma of all of the spirits studied. The spirits from Catalan Roxo are the most aromatic, with floral and fruity nuances, while the spirits from Godello were the less aromatic group as far as the varietal compounds studied are concerned. Spirits from Mencia and Treixadura show a similar profile, but the former has a more intensive aroma due to beta-pinene, citronellol, and alpha-ionone. Albari?o spirits stand out because of their profile that is marked by the contributions of eugenol and linalool. 相似文献
108.
Dry white Chinese noodle (DWCN) is widely consumed in China, and genetic improvement of DWCN quality has become a major objective
for Chinese wheat breeding programs. One hundred and four bread wheat cultivars and advanced lines, including 88from major
Chinese wheat-producing areas, were sown in two locations for two years. Their DWCN quality, as evaluated by trained panelists,
was studied to determine the relationship between wheat quality parameters and DWCN quality attributes. In general, the cultivars
and advanced lines used in this study are characterized with acceptable protein content, but accompanied with weak-medium
gluten strength and poor extensibility, and substantial variation is observed for all grain and DWCN quality characters. On
average, Australia and USA wheat performed better DWCN quality than Chinese wheats. Simple correlation analysis indicated
that both grain hardness and Farinograph water absorption were negatively associated with cooked DWCN color, appearance, smoothness,
and taste. Flour whiteness and RVA peak viscosity was positively associated with all DWCN parameters, and their correlation
coefficients (r) with DWCN score are 0.34 and 0.41, respectively. Their positive contributions to DWCN quality were mostly
through improved color, appearance, smoothness, and taste. Farinograph mixing tolerance index (MTI) and softening were negatively
associated with all DWCN quality parameters, and their correlation coefficients with DWCN score are –0.50 and–0.54, respectively.
Further analysis indicated that association between protein content, Zeleny sedimentation value, Farinograph stability, and
Extensograph extensibility, and DWCN score fit quadratic regression model significantly, with R2 0.12, 0.32, 0.22, and 0.20, respectively. The associations between Zeleny sedimentation value and DWCN's appearance and taste
also fit quadratic regression model significantly. This suggests that to certain extent, increased protein content and gluten
quality contribute positively to DWCN quality, mostly by improving palatability, elasticity, and stickiness. High flour whiteness,
medium protein content, medium to strong gluten strength and good extensibility, and high starch peak viscosity are desirable
for DWCN quality. Genetic improvement for flour whiteness, protein quality and starch paste viscosity would increase the DWCN
quality of Chinese bread wheat cultivars.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
109.
This paper reports the correlation between the rheological properties of bread wheat dough and the types and quantities of endosperm proteins in 28 common wheat cultivars. Different methods were used to analyse the allelic composition of these cultivars and the relative quantities of the different proteins contributing to the gluten structure. Neither dough strength (W) nor tenacity/extensibility (P/L) correlated with allelic composition. Different wheats with the same allelic composition (i.e., with respect to glutenins) showed different rheological properties. The glutenins were the most influential components of W and P/L, especially the high molecular weight (HMW) glutenin subunits and in particular the type x form. These proteins seem to increase W and are the main constituents of the gluten network. The gliadins and low molecular weight (LMW) glutenin subunits appear to act as a “solvent”, and thus modify the rheological properties of the dough by either interfering with the polymerisation of the HMW glutenin subunits, or by altering the relative amounts of the different types of glutenin available. Thus, the protein subunits coded for by the alleles Glu-B1x7 and Glu-D1x5 stabilised the gluten network, whereas those coded for by Glu-B1x17 and Glu-D1x2 had the opposite effect. Dough properties therefore appear to depend on the glutenin/gliadins balance, and on the ratio of the type x and type y HMW proteins. The influence of external factors seems to depend on the allelic composition of each cultivar. 相似文献
110.
de Souza Romina Peña-Fleitas M. Teresa Thompson Rodney B. Gallardo Marisa Grasso Rafael Padilla Francisco M. 《Precision Agriculture》2022,23(1):278-299
Precision Agriculture - To increase nitrogen (N) use efficiency and reduce water pollution from vegetable production, it is necessary to optimize N management. Fluorescence-based optical sensors... 相似文献