新疆绵羊致病株李斯特杆菌分离与鉴定

本研究采用生化反应，溶血试验，致病力试验，PCR方法对分离的新疆5株绵羊致病株李斯特杆菌90SB1，90SB2，90SB5，90SS1，125SL进行了种型的鉴别测定，并用RAPD技术对这5株李斯特杆菌及标准株李斯特杆菌进行分型。试验结果，五株绵羊致病株（野毒株），在生化反应，培养特性，溶血性，致病性及溶血素基因特异性等方面表现高度的一致性，属于同一种LM。RAPD实验表明此5株LM指纹图谱相同，属于同一个型。此5株致病性LM与标准LM对照株（从食品中分离到）指纹图谱差异显著，属于不同型。     

上海PMWS并发PRRS自然病例的病理组织学观察

对上海5例5～13周龄断奶仔猪多器官衰竭综合征（PMWS）并发猪繁殖与呼吸综合征（PRRS）自然病例进行了病理组织学观察。结果：5例病猪均出现坏死性淋巴结炎、间质性肺炎以及不同程度的坏死性脾炎、间质性肾炎和肝炎。2例病猪出现心肌炎，表现为心肌纤维断裂、溶解和心肌间质中淋巴细胞局灶性浸润。2例病猪出现脑炎，在大脑白质区出现神经胶质细胞结节。     

Effect of basil polysaccharide on rat model bearing NuTu-19 cells

AIM：To study the effect of basil polysaccharide on the rat model bearing NuTu-19 cells.METHODS：The rat model bearing NuTu-19 cells was treated with basil polysaccharide.The changes of the tumor cell biology and histopathology were observed.Total RNA was isolated from the tumor tissue.Hybridization assay was performed using gene expression microarray.The results were analyzed by photo disposal software-GenePix Pro3.0.RESULTS：Compared with control,the amounts of ascites in the rats treated with basil polysaccharide were decreased (P<0.05) and the scores of abdominal carcinoma metastasis were changed significantly (P<0.01).268 differentially expressed genes were found in the tumor specimen from the rats exposed to basil polysaccharide.CONCLUSION：Basil polysaccharide may be a new anti-tumor drug and it has multi-targets.Gene expression microarray has huge advantage and value in the study of the drug target.     

Effects of cinnamyl aldehyde on c-Fos and c-Myc expression in NIH3T3 cells

AIM: Cinnamyl aldehyde (CA) is one alcohol ingredient derived from Cinnamomum cassia,which is widely used in treating chronic skin wound in Chinese medicine with the curative effect of ‘rescuing YANG’.The purpose of the present study was to investigate the expression of c-Fos,c-Myc proteins at different time points in NIH3T3 treated with CA and explore the possible mechanism of promoting cell proliferation by CA.METHODS: MTT assay was used for observing cell proliferation.Expression of c-Fos and c-Myc proteins in NIH3T3 cells were assessed by immunocytochemistry assay.RESULTS: The cell proliferation was promoted obviously when CA concentration was between 8.8×10^-2 μg/L and 8.8×10 μg/L.CA at concentration of 5.5 μg/L significantly induced expression of c-Fos,c-Myc proteins at 2-3 h after the stimulation compared with control group (P<0.01).CONCLUSION: CA increases expression of c-Fos and c-Myc proteins,which may be one of mechanisms for CA to promote NIH3T3 cell proliferation.     

大兴安岭林区森工采运方式现状及发展趋势研究

本文论述了大兴安岭林区采运方式现状。分析了影响采运方式的因紊。提出了今后的发展方向。     

对伐木剪剪伐立木的力学分析及劈裂机理的研究

本文对伐木剪剪伐立木过程进行了机理分析和力学分析,对剪伐时木材的劈裂机理进行了探讨.     

犬的胃扩张与钾缺乏的相关性

胃扩张一肠扭转(gdv),常被称为臌或胃扭转,是一项复杂而容易在某些大型犬身上发生的紊乱.从临床发病迹象开始,病程发展迅速.如果这种痛苦的紊乱在两个小时内不处理,犬将受到死亡的威胁.     

基于形态学的棉花种子破损检测

以"中棉35"棉花种子为对象,应用图像处理技术,提出了基于形态学的破损识别方法.采用链码算子获取轮廓信息并进行傅里叶算子平滑,根据曲率特征定位轮廓尖端点后,通过轮廓的对称性识别局部破损棉种;基于统计思想,根据大小、形状特征的差异识别严重破损棉种.选取完好、破损棉种各200粒进行识别试验,结果表明破损棉种识别率达到94%.     

含笑新品种‘晚春含笑’

 ‘晚春含笑’是从球花含笑和云南含笑人工杂交群体中选育出来的新品种, 4—5月开花, 适应性强, 抗病虫能力强, 耐干旱, 耐贫瘠, 具有一定的耐寒能力, 适宜于庭院绿化, 亦可盆栽, 做大型盆景。     

森林资源产权制度研究——历史、问题与对策

森林资源产权制度是调整林业生产关系的基础规范和影响林业生产力发展的重要因素。在回顾和总结我国森林资源产权制度的历史和现状的基础上，分析归纳了我国森林资源产权制度存在五大问题，提出了解决问题的六项对策。