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21.
Hirofumi NAKAOKA Claude GAILLARD Takayuki IBI Yotaro SASAE Yoshiyuki SASAKI 《Animal Science Journal》2008,79(6):645-654
Heterogeneity of variance among subclasses of an effect is a potential source of bias in genetic evaluation. Degrees of the heterogeneity of variance among farm‐market‐year‐sex (FMYS) subclasses for carcass weight, beef marbling standard number, rib‐eye area, rib thickness and subcutaneous fat thickness were investigated in Japanese Black cattle. Consequences of adjusting for the heterogeneity on the predicted breeding values (PBVs) or on the genetic indexes derived from the PBVs of the five carcass traits were assessed. A total of 57 461 records were collected between 1997 and 2002 from steers and heifers fattened at farms across Japan. These records were grouped into 1591 FMYS subclasses. Bartlett's test showed that the degree of the heterogeneity of variance among the FMYS subclasses was sizeable in all traits (P < 0.0001). By applying a two‐step adjustment procedure it was possible to reduce the standard deviation, the coefficient of variation and the Gini coefficient of the phenotypic variances by 67.5% to 75.0% in the different traits. The applied adjustment caused a substantial re‐ranking of elite dams in the PBV for each trait as well as in the genetic index. This study provided evidence that the applied adjustment reduces the bias in the PBVs due to heterogeneous variances and increases the accuracy of bull‐dam selection. 相似文献
22.
SACHIHIKO ITOH ICHIRO YASUDA HARUKA NISHIKAWA HIDEHARU SASAKI YOSHIKAZU SASAI 《Fisheries Oceanography》2009,18(2):118-133
Numerical particle-tracking experiments were performed to investigate the transport and variability in environmental temperature experienced by eggs and larvae of Pacific stocks of the Japanese anchovy ( Engraulis japonicus ) and Japanese sardine ( Sardinops melanostictus ) using high-resolution outputs of the Ocean General Circulation Model for the Earth Simulator (OFES) and the observed distributions of eggs collected from 1978 to 2004. The modeled anchovy individuals tend to be trapped in coastal waters or transported to the Kuroshio–Oyashio transition region. In contrast, a large proportion of the sardines are transported to the Kuroshio Extension. The egg density-weighted mean environmental temperature until day 30 of the experiment was 20–24°C for the anchovy and 17–20°C for the sardine, which can be explained by spawning areas and seasons, and interannual oceanic variability. Regression analyses revealed that the contribution of environmental temperature to the logarithm of recruitment per spawning (expected to have a negative relationship with the mean mortality coefficient) was significant for both the anchovy and sardine, especially until day 30, which can be regarded as the initial stages of their life cycles. The relationship was quadratic for the anchovy, with an optimal temperature of 21–22°C, and linear for the sardine, with a negative coefficient. Differences in habitat areas and temperature responses between the sardine and anchovy are suggested to be important factors in controlling the dramatic out-of-phase fluctuations of these species. 相似文献
23.
Noboru SASAKI Thandar OO Yoshikazu YASUDA Takahiro ICHISE Noriyuki NAGATA Nozomu YOKOYAMA Kazuyoshi SASAOKA Keitaro MORISHITA Shouta MM NAKAYAMA Mayumi ISHIZUKA Kensuke NAKAMURA Mitsuyoshi TAKIGUCHI Yoshinori IKENAKA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1634
We developed an analytical method using an on-line column-switching liquid chromatography with triple quadrupole mass spectrometry (LC/MS/MS) for quantifying multiple steroids in serum. Using the developed method, we evaluated the serum concentration of nine steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, 21-deoxycortisol, deoxycorticosterone, progesterone, 17α-OH-progesterone and aldosterone) in dogs with hyperadrenocorticism (HAC). Serum was mixed with stable isotope internal standards and thereafter purified by the automated column-switching system. The limit of detection ranged 2–16 pg/ml for nine steroids. In the baseline samples, five steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, and 17α-OH-progesterone) were detected in all dogs. The concentrations of cortisone, 11-deoxycortisol, and 17α-OH-progesterone in dogs with HAC (n=19) were significantly higher those in dogs without HAC (n=15, P<0.02). After the adrenocorticotropic hormone stimulation test, six steroids (cortisol, corticosterone, cortisone, 11-deoxycortisol, 17α-OH-progesterone, and deoxycorticosterone) were above the limit of quantification in all dogs. Cortisol, corticosterone, cortisone, and deoxycorticosterone concentrations of dogs with HAC were significantly higher than those of dogs without HAC (P<0.02). In addition, 11-deoxycortisol and 17α-OH-progesterone concentration was higher in dogs with HAC than in dogs without HAC (P=0.044 and P=0.048, respectively). The on-line column-switching LC/MS/MS would be feasible for measuring multiple steroids in dog serum. The results suggest that cortisone, 11-deoxycortisol, and 17α-OH-progesterone would be related to HAC. Further studies are warranted to assess the clinical feasibility of steroid profile in dogs with HAC. 相似文献
24.
Elisabet TANGKONDA Meiko KUBO Satoshi SEKIGUCHI Taisuke SHINKI Satomi SASAKI Kentaro YAMADA Takako TANIGUCHI Torrung VETCHAPITAK Naoaki MISAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(8):1306
Workers in poultry abattoirs may be frequently exposed to Campylobacter jejuni, which is a leading cause of bacterial food poisoning in Japan. The present study was conducted to measure the titers of IgG and IgA antibodies against C. jejuni among 104 female workers in a chicken processing plant in Miyazaki prefecture, Japan. Information regarding habitual ingestion of raw chicken meat and potential occupational risk factors was collected using a questionnaire. Acid extracts of four C. jejuni strains representing the genotypes most dominant in Miyazaki were used as antigens. The levels of both immunoglobulins measured by ELISA were not correlated with ingestion of edible raw chicken meat, the amount consumed in one sitting, or its frequency. Although age was correlated with antibody levels, the length of employment was not. Furthermore, the IgG and IgA levels in workers at the evisceration step were significantly higher than those at other locations in the plant. To identify the bacterial proteins recognized by the workers’ IgG and IgA antibodies, Western blotting followed by LC/MS was conducted. Flagellin was identified as the common protein recognized in the sera of workers for whom ELISA demonstrated both the highest and lowest antibody levels. We concluded that the titers of IgG and IgA against C. jejuni in workers at the processing plant had been increased by occupational exposure to Campylobacter, regardless of raw chicken meat ingestion. 相似文献
25.
26.
Naoki SASAKI Yoshinori MORITA Tomoe MORIYAMA Haruo YAMADA 《Journal of Equine Science》2009,20(2):19-22
The purpose of this study was to evaluate the effect of the canine cranial cruciate
ligament repair system on laryngeal hemiplegia in heavy draft horses. Twenty-four heavy
draft horses diagnosed with grade 4 laryngeal hemiplegia were allocated to either the
prosthetic laryngoplasty (PL) group (n=14) or a canine cranial cruciate ligament repair
system (CCCLRS) group (n=10). Right to left angle quotients (RLQs) of abductions of the
arytenoid cartilages were endoscopically evaluated before and after surgery.
Post-operative RLQs in the CCCLRS group were significantly lower than those of the PL
group (P<0.01). The canine cranial cruciate ligament repair system was revealed to be a
good surgical procedure for laryngeal hemiplegia in heavy draft horses. 相似文献
27.
Takahisa YAMADA Seiki SASAKI Shin SUKEGAWA Takeshi MIYAKE Tatsuo FUJITA Hiroyuki KOSE Mitsuo MORITA Youichi TAKAHAGI Hiroshi MURAKAMI Fumiki MORIMATSU Yoshiyuki SASAKI 《Animal Science Journal》2009,80(4):486-489
Marbling, defined by the amount and distribution of intramuscular fat, is an economically important trait of beef cattle in Japan. The endothelial differentiation , sphingolipid G-protein-coupled receptor , 1 ( EDG1 ) gene has been considered as a positional functional candidate for the gene responsible for marbling. We have recently reported that 2 single nucleotide polymorphisms (SNPs), c.-312A > G in the 5' untranslated region (UTR) and c.*446G > A in the 3' UTR in EDG1 were associated with marbling in Japanese Black beef cattle, but this was not functional and a causal mutation for marbling. In the present study, we detected 2 novel SNPs, referred to as g.1475435G > A and g.1471620G > T , in the 5' flanking region of the EDG1 between low-marbled and high-marbled steer groups, which were previously shown to have EDG1 expression differences in musculus longissimus muscle. The g.1475435G > A SNP seemed not to segregate in Japanese Black beef cattle. The g.1471620G > T SNP was associated with the predicted breeding value for beef marbling standard number by the analyses using Japanese Black beef cattle population. Based on these findings, we hypothesized that the g.1471620G > T SNP might have an impact on EDG1 expression and also marbling. 相似文献
28.
Fumie MAGATA Mitsuo ISHII Eri OIKAWA Hidefumi FURUOKA Kazutaka YAMADA Naoki SASAKI Syo SHIMIZU Hisashi INOKUMA 《Journal of Equine Science》2010,21(2):17-20
A 42-day-old heavy draft horse fell into sudden astasia. Significant swelling and heat
sensation of the left femoral region were observed. Because of a friction sound in the
left hip, we supposed that the hip joint was dislocated or the hip bone was fractured.
Computed Tomography (CT) examination showed that the left hip joint was dislocated and the
left femoral head was disjunct. We carried out a pathological autopsy, and made a
diagnosis of the foal as fracture of the hip bone and femoral head with suppurative
umbilical arteritis. Pathologic changes in the umbilical artery and hind leg were
completely unilateral, suggesting that left umbilical arteritis spread to the blood
circulation, causing arthritis and dislocation of the hip bone. 相似文献
29.
Naoto WATANABE Takahisa YAMADA Sachiyo YOSHIOKA Masayuki ITOH Youichi SATOH Masako FURUTA Shigeki KOMATSU Yoshihiko SUMIO Tatsuo FUJITA Yoshiyuki SASAKI 《Animal Science Journal》2010,81(1):142-144
Our previous study detected a single nucleotide polymorphism (SNP), g.1471620G > T , in the 5' flanking region of the endothelial differentiation sphingolipid G-protein-coupled receptor 1 ( EDG1 ) gene, which has been considered as a positional functional candidate for the gene responsible for marbling, and showed association of the g.1471620G > T SNP with marbling in Japanese Black beef cattle. In the present study, we investigated the allele frequency distribution of the g.1471620G > T SNP among the 5 cattle breeds, Japanese Black, Japanese Brown, Japanese Short Horn, Holstein, and Brown Swiss breeds. The T allele at the g.1471620G > T SNP associated with high marbling was found at high frequency in Japanese Black breed that has been subjected to a strong selection for high marbling, while the allele was absent or at very low frequencies in the other breeds that have not been strongly selected for high marbling. Based on this finding, we hypothesized that the pressure of the strong selection for high marbling in Japanese Black breed has increased the frequency of the T allele at the g.1471620G > T SNP in the EDG1 . 相似文献
30.
Koichi CHIKUNI Mika OE Keisuke SASAKI Masahiro SHIBATA Ikuyo NAKAJIMA Koichi OJIMA Susumu MUROYA 《Animal Science Journal》2010,81(5):600-605
To assess the role of muscle fiber type in beef taste‐traits, we analyzed cooked meats from bovine masseter, diaphragm, psoas major, longissimus thoracis, and semitendinosus muscles with an electric taste sensing system (INSENT SA402B). The system is composed of five taste sensors of polymer membranes fixing different lipids. The sensors, CT0, CA0, AAE, C00 and AE1 are designed to respond to the individual tastes of salty, sour, umami, bitter and astringent, respectively. The system found significant differences in the converted outputs of CA0 (cvCA0), C00 (cvC00) and AE1 (cvAE1) among the bovine muscles. The slow‐type muscles (masseter and diaphragm) showed lower cvCA0, higher cvC00, and higher cvAE1 than did the fast‐type muscles (psoas major, longissimus thoracis, and semitendinosus). Lactic acid content was different among muscle types and was highly related to the cvCA0 output and pH. carbonyl compounds and free fatty acids were higher in the slow‐type muscles. Free fatty acids were major components causing the difference in the C00 output among the muscle types. Iron content was also different among the muscle types and related to the cvC00 and cvAE1 outputs. These results suggested that the muscle fiber type affects the beef taste characteristics. 相似文献