逆转录病毒介导的天蚕抗菌肽B在奶牛乳腺中的表达

为了研究天蚕抗菌肽在奶牛乳腺组织长期表达的可行性以及对大肠杆菌的抑制作用,将天蚕抗菌肽B(cecropinB,CB)的基因序列以哺乳动物偏爱的密码子优化后,合成了四条相互重叠的DNA片段,通过重叠延伸PCR法获得了天蚕抗菌肽B的基因,将其亚克隆至T栽体,测序正确后构建逆转录病毒载体pLNCX_bCP-cecB,经过包装细胞的包装,获得含有天蚕抗菌肽B表达框的逆转录病毒,注射入奶牛乳腺组织,通过琼脂板孔穴扩散法检测基因的表达及活性;实验结果表明,克隆的抗菌肽基因在乳腺中获得了表达,表达产物对大肠杆菌具有抑制作用,抑制效果可以持续至13天以上.     

蛋用鹌鹑体温变化规律新发现

为了证实蛋用鹌鹑的正常体温,掌握其生理变化的一般规律,采用双因变量多因素,2个处理6个重复的设计方案,选被试鹌鹑12只,依据性别不同分成2个处理,在不同的测定时间内,对环境温度、相对湿度、鹌鹑的翼下体温和呼吸频率进行了测定、记录、统计分析.结果显示:鹌鹑(♀,♂)平均体温为:(41.93±0.53)℃,其中雄鹌鹑(♂)的平均体温:(41.81±0.58)℃,雌鹌鹑(♀)平均体温:(42.04±0.44)℃.在昼夜之间,群体平均体温最高的时间是上午9:10,最低的时间是晚18:00,与其它畜禽的体温变化规律区别明显;研究证实了蛋用鹌鹑的正常体温,首次发现了鹌鹑特有的体温变化规律.     

利用实时荧光PCR技术检测风信子黄腐病菌

风信子黄腐病菌是我国禁止入境的病原细菌之一,我国目前尚无该病的发病报道.本研究根据风信子黄腐病菌基因组16S-23S ribosomal RNA intergenic spacer保守序列,设计并合成了1对特异性引物和1条具有稳定点突变特异性探针进行实时荧光PCR检测,风信子黄腐病菌有很强的荧光信号,供试的其它7种病原细菌菌株均没有荧光产生.与常规PCR相比,实时荧光PCR检测特异性强,灵敏度高,适合病害的快速诊断和口岸检验检疫应用.经优化反应条件,建立了稳定的风信子黄腐病菌实时荧光PCR检测方法.     

蛋用鹌鹑体温变化规律新发现

摘 要：为了证实蛋用鹌鹑的正常体温,掌握其生理变化的一般规律,设计并实施了本试验。试验采用双因变量多因素,2个处理6个复的设计方案,选被试鹌鹑12只,依据性别不同分成2个处理,在不同的测定时间内,对环境温度、相对湿度、鹌鹑的翼下体温和呼吸频率进行了测定、记录、统计分析。结果显示：鹌鹑（♀,♂）平均体温为：41.93℃±0.53℃ ,其中雄鹌鹑（♂）的平均体温：41.81℃±0.58℃,雌鹌鹑（♀）平均体温：42.04℃±0.44℃ 。在昼夜之间, 群体平均体温最高的时间是上午9:10,最低的时间是晚18:00,与其它畜禽的体温变化规律区别明显;研究证实了蛋用鹌鹑的正常体温,首次发现了鹌鹑特有的体温变化规律。     

牛气管抗菌肽在山羊乳腺组织中的表达

根据已经发表的牛气管抗菌肽（bTAP）cDNA序列设计4条短的寡核苷酸片段,以重叠延伸PCR的方法合成bTAP基因,将其克隆至pMD-18T载体,序列测定正确后,构建bTAP基因的乳腺组织特异性表达载体pBcp-bTAP,将其溶于生理盐水后直接注射山羊乳腺小叶,使bTAP基因在乳腺组织内表达,收集注射质粒前后的奶样,测试奶样对金黄色葡萄球菌,大肠杆菌的抑制能力,结果显示注射pBcp-bTAP后的奶样表现出对金黄色葡萄球菌和大肠杆菌具有明显的抑制作用,这种抑制活性可以持续至20h以上。     

世界动物卫生组织第七战略计划目标

2021年5月,世界动物卫生组织(OIE)发布了第七战略计划(2021—2025年).该计划主要目的是促进全球对动物卫生、动物福利和兽医公共卫生的认知,从而引领全球动物卫生管理工作.该计划共设5个目标:科学的专业知识、数据管理、为成员提供相关支持、与伙伴合作及OIE效率和灵活性.本文具体介绍了该计划的5个目标内容,并提...     

Effect of bioflocs on growth and immune activity of Pacific white shrimp,Litopenaeus vannamei postlarvae

The bioflocs technology (BFT) for shrimp production has been proposed as a sustainable practice capable of reducing environmental impacts and preventing pathogen introduction. The microbial community associated with BFT not only detoxifies nutrients, but also can improve feed utilization and animal growth. Biofloc system contains abundant number of bacteria of which cell wall consists of various components such as bacterial lipopolysaccharide, peptidoglycan and β‐1, 3‐glucans, and is known as stimulating nonspecific immune activity of shrimp. Bioflocs, therefore, are assumed to enhance shrimp immunity because they consume the bioflocs as additional food source. Although there are benefits for having an in situ microbial community in BFT systems, better understanding on these microorganisms, in particular molecular level, is needed. A fourteen‐day culture trial was conducted with postlarvae of Litopenaeus vannamei in the presence and absence of bioflocs. To determine mRNA expression levels of shrimp, we selected six genes (prophenoloxidase1, prophenoloxidase2, prophenoloxidase activation enzyme, serine proteinase1, masquerade‐like proteinase, and ras‐related nuclear protein) which are involved in a series of responses known as the prophenoloxidase (proPO) cascade, one of the major innate immune responses in crustaceans. Significant differences in shrimp survival and final body weights were found between the clear water and in the biofloc treatments. mRNA expression levels were significantly higher in the biofloc treatment than the clear water control. These results suggest that the presence of bioflocs in the culture medium gives positive effect on growth and immune‐related genes expression in L.vannamei postlarvae.     

重庆市50年来小麦主栽品种高分子量谷蛋白亚基分析

采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳技术（SDS-PAGE）．对重庆市近50年来主要推广的50个小麦品种的高分子量谷蛋白亚基遗传变异进行分析,检测到13种亚基和17种亚基组合类型,其中,Glu-A1有3种类型（1、null和2^＊）。分别占50％、46％和4％;Glu-B1有7种类型,主要有7＋8、14＋15和7＋9。分别占54％、18％和12％;Glu-D1有3种类型,主要是2＋12、5＋10。分别占76％、20％;品质评分在5-10之间,平均7．3分;20世纪90年代以后育成品种中优质亚基5＋10、17＋18出现频率明显增加。说明育种单位已重视利用具有优质遗传背景的材料。     

CHRIS数据几何校正精度改善研究(英文)

This paper deals with a new type of multi-angle remotely sensed data--CHRIS （the Compact High Resolution Imaging Spec- trometer）, by using rational function models （RFM） and rigorous sensor models （RSM）. For ortho-rectifying an image set, a rigorous sen- sor model-Toutin＇s model was employed and a set of reported parameters including across track angle, along track angle, IFOV, altitude, period, eccentricity and orbit inclination were input, then, the orbit calculation was started and the track information was given to the raw data. The images were ortho-rectified with geocoded ASTER images and digital elevation （DEM） data. Results showed that with 16 ground control points （GCPs）, the correction accuracy decreased with view zenith angle, and the RMSE value increased to be over one pixel at 36 degree off-nadir. When the GCPs were approximately chosen as in Toutin＇s model, a RFM with three coefficients produced the same accuracy trend versus view zenith angle while the RMSEs for all angles were improved and within about one pixel.     

Successful <Emphasis Type="Italic">Agrobacterium</Emphasis>-mediated transformation of <Emphasis Type="Italic">Populus tomentosa</Emphasis> with apple <Emphasis Type="Italic">SPDS</Emphasis> gene

The problem of salinized soils has become one of the most serious constraints to agricultural and forest productivity. With the purpose of enhancing salt stress tolerance of Populus tomentosa, we transformed this tree species with spermidine synthase （SPDS） genes derived from an apple by an Agrobacterium-mediated method. Four transgenic clones were confu＇med by PCR and Southern blot analysis. As well, the expression of introduced SPDS genes was analyzed by real-time quantitative PCR.