海南南药黎药发展研究

分析了海南南药黎药开发利用现状、发展优势及存在问题,提出海南发展南药黎药产业的思路、对策,旨在为政府有关部门和投资者提供参考。     

我国农业院校图书馆知识服务现状调查与分析

通过对国内农业院校图书馆开展知识服务现状调查发现,目前我国农业院校图书馆知识服务理念基本普及,形成了一定的知识服务构架,存在知识服务整体水平不高、互动沟通环节薄弱、区域发展不平衡、馆域间协作差等问题。因此,应因馆置宜,注重服务质量提高以及互动沟通环节建设,加强馆间协作,以确保农业院校图书馆知识服务可持续发展。     

Monitoring Indo-Pacific humpback dolphin occurrences in a highly urbanized estuary for informing conservation and management

1. The estuaries within the Pearl River Delta are sites for large-scale urban development and offshore construction. These projects have conservation stakeholders calling for more protection for the Indo-Pacific humpback dolphins whose habitat overlaps with current and proposed construction sites.
2. Efforts to improve impact assessments are hindered by the lack of baseline data and comprehensive understanding of how often the dolphins are present in areas near or within locations targeted for further development. The Modaomen Estuary within the Pearl River Delta is a good example of this, with little consideration for the dolphins as no data on their diurnal and seasonal occurrences within the estuary exist.
3. A passive acoustic monitoring system was deployed over a calendar year from October 2016 to September 2017 to monitor the presence of humpback dolphins in the Modaomen Estuary.
4. Results indicated that the estuary is an important area for humpback dolphins, with regular diel rhythms and seasonality in detection rates. For example, higher detections were seen during the wet season than during the dry season. However, there was no significant difference in detection rates between the flood and ebb tides, or between high and low tidal phases.
5. Since the Modaomen Estuary is targeted for further development in the near future, these data provide the rationale for resource management and consent processes to consider potential impacts on the dolphins, as well as to aid marine spatial planning and conservation measures.

     

白菜类富含亮氨酸重复（LRR）抗病蛋白基因BcLRR cDNA克隆及其介导的植物软腐病抗性分析

利用前期构建的软腐欧文氏菌(Erwinia carotovora subsp.carotovora)菌株BC1侵染诱导表达的大白莱(Brassica campestris subsp.pekinensis)SSH cDNA文库和cDNA芯片.在芯片分析的基础上,选取一个与拟南芥(Arabidopsis thaliana)类富含亮氨酸重复(LRR)抗病蛋白Cf-5高度同源的EST序列(GenBank登录号:DN962361)进行进一步分析.用5'-和3'-RACE方法克隆了该基因全长1 286 bp的cDNA序列,包含一个完整ORF和3'UTR,并具有Poly(A)加尾信号,将其命名为BcLRR基因(GenBank登录号:EU424347).BcLRR推导蛋白序列由327个氨基酸组成,除N端跨膜螺旋结构域外,大部分为LRR结构域,包含8个胞外LRR重复单元,由7个规范的LXXLXLXN结构和1个不规范LXXLXXXN组成.氨基酸序列比对分析发现,BeLRR蛋白与拟南芥类LRR抗病蛋白Cf-5同源性高达96.6%,与棉花(Gossypium hirsutum L.)类LRR抗病蛋白GhLRR同源性为82.3%,它们之间大部分变异限定在N端信号肽区.系统发育关系表明,在三种植物中功能均未知的这一蛋白属于一个新的胞外LRR蛋白类型,在结构上不同于其它抗性已知的胞外型LRR蛋白.将受CaMV 35S启动子驱动的BcLRR基因完整ORF序列转化拟南芥Col-0,PCR鉴定、Southern杂交和RT-PCR分析表明,BcLRR基因已整合到拟南芥基因组中,并在拟南芥中获得了转录.软腐病病情指数分析表明,转BcLRR植株提高了对软腐欧文氏菌的抗性.     

浅论《孙子兵法》谋略思想对大学古代汉语教学的启示

《孙子兵法》这部我国古代最早最伟大的兵书,作为一种能激发学生兴趣的教学资源,它的理论在选择教师、教师做好授课前的准备、教学中采用灵活多样的授课方法等方面给古汉语教学提供了有益的启示。     

地方文化资源数字化保护与文化创意品牌塑造

以惠州龙门农民画产业化为例,惠州龙门农民画是以"南蛮文化"作为独特视角,展现南国传统民俗风情的民间绘画,被誉为世界民间艺术奇葩。分析了产业化发展进程中龙门农民画的艺术特色和文化价值,阐述了基于知识管理的龙门农民画数字化保护思路,并从文化创意角度对龙门农民画的文化品牌塑造进行了探讨。     

ENDOSCOPIC ULTRASOUND INSTRUMENTATION, APPLICATIONS IN HUMANS, AND POTENTIAL VETERINARY APPLICATIONS

Endoluminal scanning under endoscopic guidance, or endoscopic ultrasonography (EUS), has become the most significant advance for imaging the gastrointestinal (GI) tract wall and contiguous organs in the past 20 years. It was originally designed to overcome the limitations in humans to imaging the abdominal organs transabdominally, such as large penetration depths and GI air. This imaging modality provides detailed images of pathological processes both within and outside of the GI wall since a high-frequency transducer can be brought into close proximity with the target regions. It has found most success in humans for the staging of lung, gastric, and esophageal cancer, the detection of both lymphatic and hepatic metastases, and diagnosis of pancreatitis and pancreatic cancer, as well as achieving an important role in interventional and therapeutic procedures. The EUS examination can be performed to examine both the thorax and abdomen in animals when both conventional transthoracic or transabdominal ultrasound are inadequate due to intervening air, bone, large penetration depths, or obesity. The echoendoscope is similar to a conventional endoscope but has an ultrasound transducer at its tip. Both radial and linear multifrequency scanners are available. Linear scanners allow fine-needle aspiration (FNA) of the bowel wall or extraluminal structures. Transducer coupling is either by direct mucosal contact or by inflation of a water-filled balloon surrounding the transducer. Current thoracic applications for EUS in veterinary medicine include examination of the mediastinum, bronchial lymph nodes, esophagus, and pulmonary lesions as well as FNA of pulmonary masses. Abdominal applications include examination of both pancreatic limbs and the liver, including portosystemic shunts, detection of lymphadenomegaly, and examination of the gastric wall, duodenum, and jejunum. Other potential applications in dogs and cats include tumor staging and intrapelvic ultrasound.     

6-BA和GA3促进三色堇再生的研究

为了解决三色堇生产周期长，观赏期短等问题，对三色堇进行了再生利用的研究。结果表明，对过期三色堇进行刈割，留茬再生，喷施6－BA和GA3，并加强肥水管理，能生产出具有良好商业价值的再生三色堇。其中喷施6－BA的花朵数多、花大、叶宽、株型适中，具有良好的观赏价值，观赏期延长1倍。