Genotype evaluation,muscle histopathology and ultrastructure in a Duchenne muscular dystrophy animal model

AIM: To evaluate the genotype , muscle histopathology and ultrastructure in dko mice. METHODS: Dystrophin/Utrophin-deficient double knockouts (dko) mice were obtained from university of Oxford, UK. Genotype of filial generation of heterozygote was evaluated by PCR-SSP. HE staining and fluorescent immunohistochemistry by SABC-Cy3 were used to detect striated muscle of dko mouse, and the muscle ultrastructure was observed by transmission electron microscope(TEM). RESULTS: In 112 filial generation mice, there were 28 mdx (25.0%), 26 dko (23.2%) and 58 heterozygote (51.8%), which coincided with the law of Mendelian inheritance. HE staining showed that the myocytes were not very uniform, there were phenomenon of round outline, centrally nucleated fibers, widening interspace, inflammatory cell infiltration and connective tissue proliferation in dko mice. There were no any immunofluorescent expression of dystrophin and utrophin in sarcolemma in dko mice. TEM showed sarcolemma breakage, separation and edema, and loose myofibril texture, inflammatory cell infiltration and connective tissue proliferation in dko mice. CONCLUSION: PCR-SSP is a very quick and accurate way for genotype evaluation of filial generation. The pathophysiology of dko mouse was very similar to Duchenne muscular dystrophy (DMD), and dko mouse is an ideal animal model for study of DMD clinical therapy.     

中国上市公司多元化经营绩效的实证分析

通过随机抽样,计算我国57家上市公司1998年-2002年的平均盈利指标和风险指标,根据各公司多元化熵值的大小对样本总体进行分组,分析多元化程度对企业绩效的影响.研究发现1.多元化程度越高,公司的平均净资产收益率越高;2.多元化经营降低了公司收益率的波动水平;3.资产规模越小的公司越倾向于选择多元化经营.     

精梳棉卷成卷过程中起泡问题分析

介绍了成卷工艺对棉卷结构的影响以及棉卷成卷过程中起泡现象与准备工艺的关系.分析了棉卷成卷工艺过程中起泡过程和起泡原因,提出了防止起泡的措施.     

草鱼免疫防病技术操作方法

草鱼免疫保证有效抗原和防止抗原流失,是免疫成功的关键;组织免疫服务队伍是持久普及推广免疫防病技术的保证措施。     

抗虫杂交棉F1、F2代纤维品质的比较研究

2003年,在山东尹庄、江苏扬州、安徽宿松、湖北荆州、湖南长沙、湖南南县、湖南澧县等7个地点,对包括对照中29在内8个组合的F1、F2代共计16个材料进行纤维品质的比较研究,结果表明:抗虫杂交棉F1和F2代间除纤维长度达到显著差异外,其他7项指标差异均无显著,但F2代纤维品质变异范围增大,劣质纤维比例增加,优良纤维比例减少,从而出现纤维品质的衰退。     

关于葡萄砧木资源的研究进展

葡萄属多年生落叶藤本植物,是葡萄科(Vitaceae)葡萄属(Vitis)植物,是地球上最古老的一种植物。据近代文献记载,葡萄多分布于温带至亚热带地区,葡萄属植物全世界约70种,我国约30种。其中仅有20多个种应用于生产果实和用作砧木,其它还处于野生状态,未被开发利用。1葡萄砧木的主要     

丰本生力酵素饲喂肉猪试验研究

选用60日龄杜洛克、长白仔猪，Ⅰ组为试验组，Ⅱ组为对照组。对照组饲养基础日粮，试验组在对照组的基础上添加1％的丰本生力酵素。结果表明：平均日增重试验组均高于对照组，其增长幅度杜洛克为13．1％，长白猪为8．12％。饲料转化率差异不显著，但瘦肉率试验组比对照组提高了13．1％。经济效益明显提高，杜洛克试验组比对照组提高了4．8％，长白猪试验组比对照组提高了5．3％。     

舍饲育肥条件下凉山半细毛羊的产肉性能研究

对三组凉山半细毛羊的 4月龄断奶公羔 ,用 3种不同的补饲水平 (Ⅰ、Ⅱ、Ⅲ )进行舍饲 ,分别于 6月龄和 8月龄对各组肥羔进行屠宰测定。结果表明高营养水平组 (Ⅰ )的羔羊 6月龄和 8月龄体质量、日增质量极显著高于低营养水平组 (Ⅲ ) (P <0 .0 1) ,显著高于中等营养水平组 (Ⅱ ) (0 .0 1

0 .0 5 )。凉山半细毛羊肌肉中氨基酸种类齐全 ,含量较丰富 ,其中必需氨基酸的总量达到 2 6 .15 %。