“阿立卡”对茶假眼小绿叶蝉的防治效果试验

试验用农药"阿立卡"针对目前茶园主要害虫之一"假眼小绿叶蝉"作了初步试验,其结果表明:"阿立卡"对茶假眼小绿叶蝉若虫有很好的防治效果,处理2(10ml/亩)、处理3(20ml/亩)在整个试验期间的校正虫口减退率均可达到100%。与对照药剂"70%吡虫啉(6g/亩)"相比,"阿立卡"持效期更长。试验初步说明了其药效作用,对茶树及其他生物的影响还有待进一步进行试验研究。     

“胰岛素-转铁蛋白-硒钠”对山羊卵母细胞体外成熟及胚胎发育的影响(英文)

[Objective] The research aimed to enhance culture efficiencies of oocyte and embryo of goat in vitro and to explore serum-free culture system in vitro.[Method] At present,the conventional solutions of oocyte maturation and embryo development in vitro were always added into 1% ITS(Insulin-transferrin-selenium) or using 1% ITS to replace FBS in 2 kinds culture solutions for conducting in vitro cultures of goat oocyte and parthenogenetic embryo.The influences of ITS on their developments were detected.[Result] ITS in maturation liquid of oocytes could not increase oocytes maturation rate but significantly increased blastocyst rate (58.06% vs. 48.19%)of parthenogenetic embryo.If FBS in maturation liquid of oocytes was replaced by ITS, the maturation rate, cleavage rate and blastocyst rate were basically unchanged.Adding ITS into embryo medium could increase blastocyst rate (68.30% vs. 56.82%)of parthenogenetic embryo of goat.If FBS in embryo medium was replaced by ITS,the cleavage rate didn’t change basically,while the blastocyst rate in ITS was obviously lower than that in FBS group(42.33% vs.56.82%).[Conclusion] ITS could promote maturation of oocyte in vitro and early embryonic development, in addition,ITS could replace serum in maturation medium of oocyte as serum-free culture system for conducting relevant researches.     

体细胞核移植法获得转人乳铁蛋白基因克隆山羊妊娠的研究(英文)

[Objective] The aim of this study is to understand the effects of donor cell type,embryo stage,number and transfer position on the efficiency of goat transgenic clone.[Method] Using somatic cell nuclear transfer technology,the single goat fetal fibroblasts(GFF)and mammary gland epithelial cells(GMGE)harboring human lactoferrin(hLF)gene were transferred to the enucleated oocyte.Reconstructed karyoplast-cytoplast couplets were fused,activated,and cultured in vitro.Embryos at 2-8 cell stage were transferred into oviduct of synchronized recipients,and blastocysts were transferred into uterine horn.[Result] The pregnancy rate was similar between GFF and GMGE(oviduct transfer:26.47% vs.20.00%),and between oviduct transfer and uterine horn transfer(26.47% vs.25.00%)for GFF group;pregnancy rate in the group with the mean number of embryo transferred per recipient of 21.2 was significantly higher than in those the 5.93 group and 9.64 group(40.00% vs.26.67% and 21.43%).[Conclusion] These results indicate that pregnancy rate of goat transgenic clone couldn’t be affected by donor cell type,embryo stage and transfer position but be done by the number of embryo transferred per recipient.In addition,the study also suggests the feasibility of making transgenic goat using GMGE as donor cells.     

日本海水养殖鱼类可追溯体系建设

日本是水产品的生产和消费大国,养殖渔业更承担着持续稳定地提供水产食品的任务。随着近年水产品质量问题的增多,消费者越来越关注水产品的质量安全问题,日本政府和相关各方把建立水产品可追溯体系视为减少水产品质量不安全性和增强消费者信心的有力武器,采取法律法规支撑、政府鼓励和各方自愿的原则,积极建立包括海水养殖鱼类在内的水产品可追溯体系。     

干旱及复水对荒漠绿洲过渡带一年生草本植物生长及生理特性的影响

一年生草本植物种群组成荒漠与绿洲生态系统的恒有性植物层片,在防风固沙和保护绿洲生态系统稳定方面具有重要的作用。本研究选取河西走廊荒漠绿洲过渡带人工林下典型5种一年生草本植物虎尾草、狗尾草、白茎盐生草、沙米和雾冰藜为研究对象,采用盆栽试验模拟干旱胁迫及复水,测定5种植物幼苗根系和叶片的生长（株高、根体积、根系平均直径、根系总表面积、根系总长和根系干重）与生理指标（根系活力、光合色素、丙二醛、渗透调节物质和抗氧化酶活性）的变化特征和规律,分析其对干旱环境的适应能力。研究结果表明,轻度干旱胁迫（土壤水分相对于CK减少2%）对5种一年生草本植物生长影响较小,甚至有一定的促进作用;中度（土壤水分相对于CK减少4%）和重度（土壤水分相对于CK减少6%）干旱胁迫下植物生长受到明显抑制。随着干旱胁迫程度的加剧,5种植物根系和叶片的丙二醛、过氧化物酶活性和渗透调节物质含量呈上升趋势;而根系活力呈下降趋势。禾本科一年生草本植物虎尾草和狗尾草根系酶活性（超氧化物歧化酶）随干旱胁迫程度的加剧呈持续增加的趋势;藜科一年生草本植物沙米和雾冰藜随干旱胁迫程度的加剧表现出先增加后下降的趋势。复水处理后,5种植物的生长...     

深松整地对小麦的增产效果

<正>对旱地进行机械深松整地,能够疏松耕层土壤,打破犁底层,很好地改良土壤的通透性,通气透水,抗旱防涝,有利作物根系生长发育,促进分蘖,提高作物产量。自2015年起,湖北省农机局按照农业部要求,在全省适宜深松的地方开展机械深松整地工作,2016年枣阳市承担深松整地任务25万亩(1hm~2=15亩)。为摸清深松整地对小麦的增产效果,以便大面积推广,笔者开展了深松与未深松对比示范。1材料和方法     

60型散热器冻裂后如何修复

去年一次停电事故,使我厂200多片散热器被冻裂。拆掉后发现被冻坏的散热器有90％以上都是在散热器中间的二根拉结筋和外壳的连接处,由于冻胀而被拉开的。裂口呈圆形(φ30～50mm)。开始我们用电焊进行焊接,但由于铸铁内部应力作用,焊后就有一半以上开裂,剩下的一半使用一段时间后,又有很多开裂了。