1. The increase in microbial resistance, and in particular multiple drug resistance (MDR), is an increasing threat to public health. The uncontrolled use of antibiotics and antibacterial chemotherapeutics in the poultry industry, especially in concentrations too low to cause inhibition, and the occurrence of residues in feed and in the environment play a significant role in the development of resistance among zoonotic food-borne microorganisms.
2. Determining the presence and transmission methods of resistance in bacteria is crucial for tracking and preventing antibiotic resistance. Horizontal transfer of genetic elements responsible for drug resistance is considered to be the main mechanism for the spread of antibiotic resistance.
3. Of the many well-known genetic elements responsible for horizontal gene transfer, integrons are among the most important factors contributing to multiple drug resistance. The mechanism of bacterial drug resistance acquisition through integrons is one of the essential elements of MDR prevention in animal production.
The objective of this study was to evaluate the efficacy of potassium diformate (KDF) as a potential additive for alfalfa silage. Fresh alfalfa was untreated or treated with formic acid (4 g/kg fresh weight, FW) or three concentrations of KDF (4, 5.5 or 7 g/kg FW). After 60 days of ensiling, the addition of formic acid and greater levels of KDF (5.5 and 7 g/kg) effectively reduced silage pH and inhibited the undesirable bacteria, indicated by lower butyric acid, ethanol, ammonia N concentrations and microbial populations (including enterobacteria, yeasts, moulds and clostridia). Additives decreased the dry‐matter loss, and more water‐soluble carbohydrates were preserved in the silages with formic acid or potassium diformate than in the control. Alfalfa silages treated with formic acid at 4 g/kg FW or potassium diformate at 5.5 or 7 g/kg FW were classified as the highest quality silage based on the higher Flieg's point (above 70) and remained stable for more than 9 days during aerobic exposure. Potassium diformate is recommended as an effective additive for alfalfa silages at a level of 5.5 or 7 g/kg FW under the humid and hot conditions of southern China. 相似文献
Late blight, caused by the oomycete Phytophthora infestans (Mont.) de Bary, is a devastating disease in potato and tomato and causes yield and quality losses worldwide. The disease first emerged in central America and has since spread in North America including the United States and Canada. Several new genotypes of P. infestans have recently emerged, including US-22, US-23 and US-24. Due to significant economic and environmental impacts, there has been an increasing interest in the rapid identification of P. infestans genotypes. In addition to providing details regarding the various phenotypic characteristics such as fungicide resistance, host preference, and pathogenicity associated with various P. infestans genotypes, information related to pathogen movement and potential recombination may also be determined from the genetic analyses. Restriction fragment length polymorphism (RFLP) analysis with the RG57 loci is one of the most reliable procedures used to genotype P. infestans. However, the RFLP procedure requires propagation and isolation of the pathogen and relatively large amounts of DNA. Isolation of the late blight pathogen is sometimes impossible due to the poor condition of the infected tissues or the presence of fungicide residues. In this study, we describe a procedure to identify P. infestans at the molecular level in planta using terminal restriction fragment length polymorphism (T-RFLP) of the RG57 loci. This T-RFLP assay is sufficiently sensitive to detect and differentiate P. infestans genotypes directly in planta without propagation and isolation of the pathogen, to facilitate the timely implementation of best management practices. 相似文献
Eurasian Soil Science - Data on concentrations and distribution of tritium in soils of the “Atomic” Lake excavation explosion area of Semipalatinsk Nuclear Test Site (Kazakhstan, East... 相似文献
白粉病是瓜类作物重要病害之一。本研究采用苗期人工接种方法,研究了117份瓠瓜微核心种质对白粉病的抗性,并基于不同材料的病情指数和基因型数据,进行全基因组关联分析(genome-wide association analysis,GWAS)。利用2014年和2018年的抗病表型数据,分别鉴定到22个和13个与白粉病抗性相关的SNPs,其中2个SNPs位点在两年试验中同时被检测到。进一步研究发现不同瓠瓜材料的白粉病抗性与其携带有利等位变异数目成正相关。本研究为克隆瓠瓜抗白粉病相关基因、研究其抗病机制提供科学依据,促进了瓠瓜抗白粉病分子育种。 相似文献