不同植被措施下排土场边坡细沟发育时空特征

为明确不同恢复年限植被措施对煤矿排土场边坡水土流失的防治效应,采用样方调查法,以内蒙古准格尔旗永利煤矿矿区排土场边坡为研究对象,以裸露边坡(CK)为对照,研究不同恢复年限(1a、3a、5 a)的沙柳方格+沙棘+沙打旺(SHA)和沙柳方格+沙打旺(SA)2种措施对细沟发育的影响。结果表明:(1)1a时CK、SHA与SA措施细沟宽度均集中分布在4～8 cm,细沟深度均集中分布在2～4 cm;3 a时CK、SHA与SA措施细沟宽度则集中分布在4～8 cm、8～12 cm、4～8 cm,细沟深度均集中分布在4～6 cm;5 a时CK与SHA措施细沟宽度均集中分布在8～12 cm,而细沟深度则集中分布在4～6 cm和8～14 cm;(2)CK(1～5 a)、SHA措施(1～5 a)和SA措施(1～3 a)的细沟平均宽分别为7.57～11.35 cm、7.58～13.31 cm和5.57～6.14 cm,细沟平均深分别为3.38～6.23 cm、4.19～10.34 cm、2.59～4.24 cm,三者的细沟平均宽深比分别为2.39、2.12和2.05,平均细沟密度分别为1.52～5.25 m·m–2、1.42～1.68 m·m–2和1.88～2.25 m·m–2;(3)1 a时CK、SHA和SA措施的细沟宽深比随坡长变化幅度较大,随着恢复年限增加,宽深比则呈下降趋势,CK、SHA措施和SA措施的细沟密度和细沟侵蚀量均随坡长增加呈增大趋势;(4)与CK相比,1 a时SHA和SA措施边坡细沟侵蚀模数分别减小25.0%和25.86%,两种措施减蚀效果差别不大,而3 a时则分别减小了61.73%和35.31%,SHA措施减蚀效果显著增强。研究结果可以为矿区排土场边坡的植被合理布设提供科学依据与理论指导。     

EPS364, a Novel Deep-Sea Bacterial Exopolysaccharide,Inhibits Liver Cancer Cell Growth and Adhesion

The prognosis of liver cancer was inferior among tumors. New medicine treatments are urgently needed. In this study, a novel exopolysaccharide EPS364 was purified from Vibrio alginolyticus 364, which was isolated from a deep-sea cold seep of the South China Sea. Further research showed that EPS364 consisted of mannose, glucosamine, gluconic acid, galactosamine and arabinose with a molar ratio of 5:9:3.4:0.5:0.8. The relative molecular weight of EPS364 was 14.8 kDa. Our results further revealed that EPS364 was a β-linked and phosphorylated polysaccharide. Notably, EPS364 exhibited a significant antitumor activity, with inducing apoptosis, dissipation of the mitochondrial membrane potential (MMP) and generation of reactive oxygen species (ROS) in Huh7.5 liver cancer cells. Proteomic and quantitative real-time PCR analyses indicated that EPS364 inhibited cancer cell growth and adhesion via targeting the FGF19-FGFR4 signaling pathway. These findings suggest that EPS364 is a promising antitumor agent for pharmacotherapy.     

Construction and evaluation of introgression lines and fine mapping of ehd8 from Jinghong common wild rice (Oryza rufipogon)

Heading date is one of the most important traits in rice and regulated by multiple genes. Common wild rice is the ancestor of Asian cultivated rice and harbours abundant genetic diversity. To use wild rice resource in rice breeding, a set of 154 introgression lines (ILs) covering 93% of the genome of Jinghong common wild rice was constructed in the background 'Teqing', using 208 simple sequence repeat markers evenly distributed on 12 chromosomes. Among the ILs, the line JIL64 displayed late heading independent of photoperiod. Genetic analysis using the two F₂ populations crossed ''Teqing'/JIL64 and JIL64/'Teqing' revealed that late flowering was controlled by a recessive gene on chromosome 8 (designated early heading date 8, ehd8), and ehd8 was fine mapped to the 50‐kb region flanked by markers RM22221 and 64Indel4. Sequencing and qRT‐PCR demonstrated that LOC_Os08g01410 and LOC_Os08g01420 were deleted in JIL64 and may be associated with the late heading of Jinghong common wild rice. These findings lay a practical foundation for characterizing ehd8, and the ILs help to mine genes from Jinghong common wild rice.     

水霉属菌(Saprolegnia)分离培养技术研究

 对水霉(Saprolegnia)的分离培养技术进行了探索,筛选出大麻籽、油菜籽、红花籽、苍蝇、蚜虫、小麦粒、玉米片、茶叶、葵花仁9种材料作为诱发水霉菌的诱饵。其中油菜籽能防止腐霉菌(Pythium)污染,是初步纯化菌种、排除杂菌的适宜饵料。同时发现控制温度能使水霉在最短时间内分别产生无性或有性繁殖。在水培条件下,25℃左右适合菌丝及无性器官的发育,有性器官却不能产生,而只有在15~17℃条件下才能使水霉很快进入有性生殖阶段;在加入200mg/L链霉素的CMA培养基上促发有性阶段则需要在25℃左右的温度下进行,方能缩短培养时间。     

导入AGPase基因的转基因可育水稻及其经济性状的研究

以农杆菌介导法将淀粉合成关键酶AGPase(腺嘌呤二磷酸葡萄糖焦磷酸化酶）基因导入云南地方优质水稻合系35，38，39，41，42中，获得了大量的可育转基因植株。PCR扩增检测，外源基因已稳定整合进水稻基因组中。大量的农艺性状及淀粉含量研究表明，转基因水稻种子的千粒重比对照明显提高，但总淀粉含量没有显著变化。     

退耕还林管理模式的创新

退耕还林工程是我国正在实施的林业重点生态工程之一,依靠政策牵引、行政推动、严格检查的管理模式很难使这项涉及面宽、运行复杂的重大工程快速持续地推进.工程的实施应根据时代发展的要求,在人本主义理念的指导下,构建以激励机制为核心的市场化管理模式.     

薛家湾镇道路绿化模式研究

在对薛家湾镇现有的11条主、次干道路的总体绿化状况实地调查的基础上,对薛家湾镇的开源路、蓝天路、塔哈拉路道路绿化模式进行研究,并提出设计方案,为提高薛家湾镇生态环境质量、改善城市面貌提供参考依据。     

国内高架桥绿化及研究现状

从高架桥区的生态环境、适生种类的选择以及高架桥绿化的养护管理、景观配置模式四个方面对国内的高架桥绿化及研究现状进行了分析和探讨,指出了存在的问题和解决方法,并对高架桥绿化的前景进行了展望.     

龙竹竹材吸湿膨胀特性研究

对龙竹竹材的吸湿膨胀特性进行了测试和分析。结果表明,竹材各个方向上吸湿膨胀特性差异显著,且竹间部位和竹节部位的吸湿膨胀特性也有一定差异。     

我省胶合板工业发展的探讨

本文在分析了胶合板市场发展前景和云南胶合板生产现状的基础上，对建立云南胶合板用材林基地；提高产品质量，生产适销对路的规格品种；提高技术水平，完善生产装备；加强经营管理，降低生产成本等方面提出了有益的建议。