Pelargonium flower-break and pelargonium line pattern viruses in the Netherlands; purification,antiserum preparation,serological identification,and detection in pelargonium by ELISA

Two viruses, detected frequently in the Netherlands in pelargonium, were identified by serology and test plant reactions. Antisera were prepared and an ELISA procedure was developed to detect the viruses in pelargonium.One of the viruses, PFBV-N, proved to be pelargonium flower-break virus. With the antiserum to PFBV-N, it could be detected reliably throughout the year inPelargonium zonale Springtime Irene.The other virus, PLPV-N, was serologically closely related to pelargonium line pattern virus (PLPV) and to pelargonium ring pattern virus (PRPV), as were an old virus isolate from Saturnus, collected in the Netherlands in 1971 (L128), and PLPV isolates from Yugoslavia (PLPV-Y) and Denmark (PLPV-D). There were only minor differences in host-plant reactions between the virus isolates. Based on these tests, PLPV and PRPV are considered as isolates of the same virus, for which, for practical reasons, the name pelargonium line pattern virus is proposed.PLPV could be reliably detected by ELISA inP. zonale Springtime Irene and Amanda throughout the year with only a few exceptions. InPelargonium peltatum Tavira, however, reslts were erratic due to uneven distribution of virus in the plant. Best results were obtained when petioles of fully expanded leaves were tested.     

Frequency of transmission of artichoke Italian latent nepovirus by Longidorus fasciatus (Nematoda: Longidoridae) from artichoke fields in the Iria and Kandia areas of Argolis in northeast Peloponnesus, Greece

Artichoke Italian latent nepovirus (AILV) transmitted by Longidorus fasciatus is a causative agent of artichoke patchy chlorotic stunting (APCS) in northeast Peloponnesus in southern Greece. Populations of L. fasciatus collected from the Iria and Kandia areas of Argolis in northeast Peloponnesus were used in laboratory experiments to determine the frequency of transmission of the virus by its natural vector. One tenth to almost one half of the L. fasciatus specimens recovered from soil collected in two artichoke fields showing APCS transmitted AILV. Allowing nematodes access for 4 wk to Nicotiana clevelandii mechanically infected with AILV did not increase the number of individual specimens able to transmit virus. The total number of specimens transmitting virus in an experiment did not exceed fifty percent of the individuals tested. Virus-like particles were only observed adsorbed to the inner surface of the odontostyle and it is suggested that the high frequency of transmission of AILV by L. fasciatus is a result of efficient dissociation of virus particles from the specific sites of retention in the vector.     

北方半湿润滨海地区海水养殖废水灌溉菊芋研究

2007年3~10月,在山东莱州海洋863中试基地,研究了海水养殖废水灌溉条件下,耐盐能源植物菊芋的盐胁迫响应以及对氮、磷的吸收利用机制。结果表明:海水养殖废水灌溉条件下,菊芋地上部生物量(茎、叶)随着灌溉水盐度(S)的增加明显下降,盐度为9.46 g/L的养殖废水处理生物量仅为淡水处理(CK2)的62.5%;地下部生物量(根、块茎)与盐度之间的关系符合一元二次方程曲线,且当盐度为3.83 g/L时,地下部生物量取得最大值(0.205 kg/株)。养殖废水处理菊芋地上部氮浓度明显低于淡水处理,地下部则各灌溉水处理间氮浓度没有明显差异;磷浓度地上部各灌溉水处理间没有明显差异,但地下部养殖废水灌溉处理明显高于淡水处理,表明海水养殖废水灌溉可以促进菊芋对磷的吸收,但氮利用受到显著影响。通过氮、磷通量计算,进一步证实了这一结果。     

菊芋的功能及其在食品中的应用

菊芋是一种具有多种生理功能的天然绿色食品,极具开发价值。论述了菊芋的营养功能、生理功能、生态功能及其在食品中的应用。     

中国沿海半干旱地区海水养殖废水灌溉耐盐植物(菊芋)的研究

An experiment with six treatments： CK1 （rainfed）, CK2 （irrigated with freshwater）, and 4 treatments of saline aquaculture effluent blended with brackish groundwater at different ratios of 1：1, 1：2, 1：3, and 1：4 （v/v） was carried out during 2004 to assess the effect of saline aquaculture effluent on plant growth and soil properties in the Laizhou region, Shandong Province, China and to determine an optimal salinity threshold for aquaculture effluent. Cumulative evapotranspiration for the saline aquaculture effluent irrigation and non-irrigation treatments was lower than that for the freshwater irrigation treatment. Soil electrical conductivity was higher with respect to saline aquaculture effluent irrigation treatment compared to that with respect to non-irrigation or freshwater irrigation treatment. For Jerusalem artichoke （Helianthus tuberosus L.）, in comparison to the freshwater treatment, plant height and aboveground biomass for the 1：3 and 1：4 treatments were constrained, whereas stem width and root biomass were enhanced. Concomitantly, higher tuber yield was obtained for the 1：3 and 1：4 treatments compared to that for CK1 and 1：1 treatments. Nitrogen and phosphorus were higher in tubers of the 1：4 treatment. This study demonstrated that saline aquaculture effluent could be used successfully to irrigate Jerusalem artichoke with higher tuber yield and nutrient removal.     

播期与密度对沙地菊芋产量的影响

本试验采用播期与栽培密度两因素的裂区设计,以确定沙地菊芋种植的较佳密度和适宜播期。研究表明,不同播期与密度交互作用对沙地菊芋地上部植株植物学性状无显著影响;而对于块茎性状及产量,春播优于秋播,密度以25500株/hm2为宜。     

Influence of Organic Manures on Soil Characteristics and Yield of Jerusalem Artichoke

ABSTRACT

The objective of this study was to study the influence of three organic manures, farm yard manure (FYM), poultry manure (PLM), and pigeon manure (PGM), on soil physical and chemical properties on tuber yield of Jerusalem artichoke in a newly reclaimed saline calcareous soil. A field experiment was conducted applying the three manures, alone and/or in different combinations. Soils were investigated at surface (0–30 cm) and subsurface (30–60 cm) layers before and after planting, and analyzed for physical and chemical properties. The results indicated that the application of 31.5 kg ha^?1 of PLM+10.5 kg of PGM T7 recorded highest available nitrogen, zinc, copper, and moisture content at the surface layer. The same results were obtained for iron and manganese at both layers. While, applying 21.0 kg ha^?1 FYM+21.0 kg ha^?1 PLM T10 recorded the best treatment for pH, phosphorus, zinc, copper, moisture content, and saturation percentage at the subsurface layer. Applying 21.0 kg ha^?1 PGM + 10.5 kg ha^?1 FYM+10.5 kg ha^?1 PLM T15 recorded the best treatment for organic matter content and bulk density at surface layer and reduced the electrical conductivity and inulin tuber content at both layers. On the other hand, calcium carbonate and sodium adsorption ratio were reduced in both layers by applying 21.0 kg ha^?1 PLM+10.5 kg ha^?1 FYM+10.5 kg ha^?1 PGM T14. The best treatment for tuber nitrogen content and total yield was obtained with applying 42.0 kg ha^?1 PLM T2 only and 31.5 kg ha^?1 FYM+10.5 kg ha^?1 PLM T4, respectively.     

全生育期菊芋不同器官果聚糖积累分配特征研究

以“青芋2号”和“青芋3号”菊芋为试材,研究了整个生长周期内菊芋不同器官中以果聚糖为主的碳水化合物含量变化动态特征以及在各器官的分配情况.结果表明:苗期叶、茎中果聚糖含量和总量增加迅速,主要贮存在叶中;植株迅速生长期和块茎形成期叶、茎中果聚糖含量呈起伏变化;全株果聚糖积累总量呈持续上升趋势,主要贮存在叶和茎中;开花期叶、茎中的果聚糖含量和积累总量先后达到全生育期的最高值,并完成果聚糖从叶片向茎的转运;块茎内含量稳定,积累总量增加缓慢;果聚糖主要贮存在茎内;块茎膨大期,全株的果聚糖积累量增加缓慢,以植株内部转运为主,果聚糖开始迅速从茎向块茎分配,到生育期末,全株果聚糖70％分配到块茎.     

华北平原海水灌溉对土壤性质和菊芋产量的影响

Irrigation with various dilutions of seawater can act as an alternate water resource and thus plays an important role in saving freshwater resources as well as promoting agriculture in the coastal semi-arid areas of the North China Plain. Jerusalem artichoke （Helianthus tuberosus L.） grown in a field experiment was irrigated with seawater diluted with freshwater from 2001 to 2003 to determine the feasibility of seawater irrigation in the Laizhou area. For treatments of CK （non-irrigation） along with seawater concentrations of 25%, 50%, and 75%, total dissolved solid （TDS） in the non-irrigated soil significantly increased （P ≤ 0.05） in both 2002 and 2003 and was 1.3 times higher in 2003 than in 2001. In the 25% and 50% seawater concentration treatments, TDS in 2001 was significantly greater （P ≤ 0.05） than CK; however, TDS in these two treatments decreased by 34.9% and 40.1%, respectively, in 2003 compared with 2001. The sodium adsorption ratio （SAR） remained below 10 mmol^1/2 L^-1/2, indicating that alkalization was low with seawater irrigation. In 2001 and 2002, compared to CK and the irrigation treatment with 75% seawater, irrigation with 25% and 50% seawater increased the yields of Jerusalem .artichoke. This meant that Jerusalem artichoke could be safely grown in salt-affected land of Laizhou area with 25% and 50% seawater irrigation.     

朝鲜蓟发酵茶泡制工艺研究

以感官评价为指标,通过单因素试验和响应面试验设计优化朝鲜蓟发酵茶泡制工艺。结果表明,朝鲜蓟发酵茶的最佳泡制工艺为冲泡时间22 min,加水量243 mL,冲泡水温86℃,粉碎度40目,在此条件下感官评分为90分。通过ABTS法和DPPH法检测表明,朝鲜蓟袋泡茶具有明显的抗氧化能力。