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The Eurasian wild boar (Sus scrofa) is a reservoir for tuberculosis (TB) in which vaccination is a valuable tool for control. We evaluated the protection and immune response achieved by homologous and heterologous regimes administering BCG and heat-inactivated Mycobacterium bovis (IV). Twenty-one wild boar piglets were randomly allocated in five groups: Control, homologous BCG, homologous IV, heterologous IV-BCG, heterologous BCG-IV. Significant 67% and 66% total lesion score reductions were detected in homologous IV (IVx2) and heterologous IV-BCG groups when compared with Control group (F4,16 = 6.393, p = 0.003; Bonferroni Control vs IVx2 p = 0.026, Tukey Control vs IV-BCG p = 0.021). No significant differences were found for homologous BCG (although a 48% reduction in total lesion score was recorded) and BCG-IV (3% reduction). Heterologous regimes did not improve protection over homologous regimes in the wild boar model and showed variable results from no protection to similar protection as homologous regimes. Therefore, homologous regimes remain the best option to vaccinate wild boar against TB. Moreover, vaccine sequence dramatically influenced the outcome underlining the relevance of studying the effects of prior sensitization in the outcome of vaccination. 相似文献
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姜瘟病是中国生姜生产上发生较为普遍的病害,是由青枯劳尔氏菌(Ralstoniasolanacearum)引起的。此病原菌寄主范围广,可侵染44个科300多种植物。菌体短杆状,在TZC选择性培养基上培养出现白边较宽和白边较窄的两种菌落。菌系划分为5个生理小种和5个生化型,中国的姜瘟菌主要属生理小种1号,生物型属II、III、IV。此病害是一种较难防治的土传病害,在高温、高湿、雨水多、氮肥过量的情况下发生较为严重。中国植病工作者对此病害作了大量的研究工作,对中国姜瘟病的病原菌、发病规律、及防治等研究进展作一综述。 相似文献
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张强 《湖南农业大学学报(自然科学版)》2001,(2)
银行业网络化的出现 ,已不再是一种单纯的金融创新 ,它将引发一场金融业、乃至整个经济局势的重大变革 ,这是历史赋予我们的一次绝好发展机遇 ,我们必须对这个问题引起高度重视。监管问题将是未来网络银行发展中一个十分重要的方面。目前我国网络银行发展水平较低 ,因此 ,必须以发展眼光来充分认识网络银行发展中监管面临的新问题 ,积极作好应对策略 相似文献
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Composting and thermal drying are amongst the most commonly used post-digestion processes for allowing sanitation and biological stabilization of sewage sludge from municipal treatment plants, and making it suitable as soil conditioner for use in agriculture. To assess the impact of sludge-derived materials on soil microbial properties, fresh (LAF), composted (LAC) and thermally dried (LAT) sludge fractions, each resulting from a different post-treatment process of a same aerobically digested sewage sludge, were added at 1% (w/w) application rate on two contrasting (a loam and a loamy sand) soils and incubated under laboratory conditions for 28 days. Soil respiration, microbial ATP content, hydrolytic activities and arginine ammonification rate were monitored throughout the incubation period. Results showed that soil biochemical variables, including the metabolic quotient (qCO2), were markedly stimulated after sludge application, and the magnitude of this stimulatory effect was dependent on sludge type (precisely LAT > LAF > LAC), but not on soil type. This effect was related to the content of stable organic matter, which was lower in LAT. Genetic fingerprinting by PCR–DGGE revealed that compositional shifts of soil bacterial and, at greater extent, actinobacterial communities were responsive to the amendment with a differing sludge fraction. The observed time-dependent changes in the DGGE profiles of amended soils reflected the microbial turnover dependent on the sludge nutrient input, whereas no indications of adverse effects of sludge-borne contaminants were noted. Our findings indicate that composting rather thermal drying can represent a more appropriate post-digestion process to make sewage sludge suitable for use as soil conditioner in agriculture. 相似文献
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随着新媒体时代的到来,人们接受信息的途径也有了全新的变化。新媒体给公共图书馆带来了机遇和挑战,公共图书馆面对种种挑战,积极创新服务,增强服务能力,提高服务水平和质量。 相似文献
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为探讨布氏乳杆菌和甲酸对青藏高原不同物候期燕麦(Avena sativa L.)青贮饲料发酵品质和细菌群落的影响,本试验以开花期、乳熟期和蜡熟期燕麦为原料,设置对照、添加甲酸和添加布氏乳杆菌3个处理,青贮90 d后,测定其营养成分、发酵指标和细菌群落组成。结果表明:布氏乳杆菌和甲酸处理均改变不同物候期燕麦青贮饲料中细菌群落组成(P<0.05),并提高其乳酸细菌相对丰富度;与对照相比,用布氏乳杆菌和甲酸处理不同物候期的燕麦青贮后,蜡熟期的燕麦pH值降低(P<0.05),乙酸均增加9.7%,丙酸分别降低11.6%和8.6%,氨态氮分别降低11.3%和19.2%。综合细菌群落组成、发酵品质和营养成分来看,蜡熟期的燕麦添加布氏乳杆菌和甲酸后,其青贮品质较佳。 相似文献
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Intensive land use practices necessary for providing food and raw materials are known to have a deleterious effect on soil. However, the effects that such practices have on soil microbes are less well understood. To investigate the effects of land use intensification on soil microbial communities we used a combined T-RFLP and pyrosequencing approach to study bacteria, archaea and fungi in spring and autumn at five long term observatories (LTOs) in Europe; each with a particular land use type and contrasting levels of intensification (low and high). Generally, due to large gradients in soil variables, both molecular methods revealed that soil microbial communities were structured according to differences in soil conditions between the LTOs, more so than land use intensity. Moreover, variance partitioning analysis also showed that soil properties better explained the differences in microbial communities than land use intensity effects. Predictable responses in dominant bacterial, archaeal and fungal taxa to edaphic conditions (e.g. soil pH and resource availability) were apparent between the LTOs. Some effects of land use intensification at individual field sites were observed. However, these effects were manifest when land use change affected soil conditions. Uniquely, this study details the responses of different microbial groups to soil type and land use intensification, and their relative importance across a range of European field sites. These findings reinforce our understanding of drivers impacting soil microbial community structure at both field and larger geographic scales. 相似文献