桉树不同成熟度叶片营养元素内循环特征

揭示桉树人工林不同成熟度的叶片器官所需营养元素变化以及土壤肥力对其影响,对于桉树营林和科学施肥具有重要的生产指导意义。选取广西隆安县小林镇和田东县思林镇的桉树2代萌芽林作为研究对象,测定两个人工林嫩叶、成熟叶、黄叶的C、N、P、K、B、Ca、Mg以及土壤C、N、P、K、Ca、Mg元素含量,并进行相关分析。结果表明,植物叶片的N、P、K、Mg营养元素转移规律是黄叶向成熟叶和嫩叶转移,但C、Ca、B营养元素则是随着叶片的生长发育逐渐积累,黄叶中含量最高,说明施肥时应关注Ca、B元素的持续和有效供给。两片人工林都表现出类似的规律,说明土壤肥力的差异不影响桉树人工林叶片营养元素的内循环规律。桉树嫩叶营养元素含量及其生态化学计量比学特征具有与成熟叶和黄叶不同的表征,在营养物质研究中应予以足够重视。     

Development of a core set of KASP markers for assaying genetic diversity in Brassica rapa subsp. chinensis Makino

Single‐nucleotide polymorphisms (SNPs) are rapid, economical and reliable genotyping tools. Non‐heading Chinese cabbage (Brassica rapa L. subsp. chinensis Makino) is now an economically important vegetable crop worldwide. In this study, 1,167 SNPs were evaluated for 7polymorphism among 70 representative non‐heading Chinese cabbage inbred lines using a Kompetitive Allele Specific PCR (KASP) genotyping assay. On the basis of identified polymorphisms and the results of a principal component analysis, we selected 50 core SNPs that were balanced sufficiently to provide adequate information for genetic identification. The core SNPs were used for construction of a neighbour‐joining dendrogram that separated the 70 inbred lines into four main groups and several subgroups corresponding to Caixin, Heiyebaicai, Huangxinwu, Naibaicai, Taitsai, Pak‐choi, and Wutatsai. This categorization was superior to that achieved using a dataset of 479 polymorphic SNPs. To confirm the utility of the core SNP markers in genetic identification, we tested their stability and resolution using 162 commercial hybrid cultivars. The SNPs, which represent a cost‐effective, accurate marker set for germplasm analysis and cultivar identification, are suitable for molecular marker‐assisted breeding in non‐heading Chinese cabbage.     

Revisiting rice breeding methods – evaluating the use of rapid generation advance (RGA) for routine rice breeding

Rice production needs to increase in the future in order to meet increasing demands. The development of new improved and higher yielding varieties more quickly will be needed to meet this demand. However, most rice breeding programmes in the world have not changed in several decades. In this article, we revisit the evidence in favour of using rapid generation advance (RGA) as a routine breeding method. We describe preliminary activities at the International Rice Research Institute (IRRI) to re-establish RGA on a large scale as the main breeding method for irrigated rice breeding. We also describe experiences from the early adoption at the Bangladesh Rice Research Institute. Evaluation of RGA breeding lines at IRRI for yield, flowering time and plant height indicated transgressive segregation for all traits. Some RGA lines were also higher yielding than the check varieties. The cost advantages of using RGA compared to the pedigree method were also empirically determined by performing an economic analysis. This indicated that RGA is several times more cost effective and advantages will be realized after 1 year even if facilities need to be built. Based on our experience, and previous independent research empirically testing the RGA method in rice, we recommend that this method should be implemented for routine rice breeding in order to improve breeding efficiency.     

浙江 江西两省矿业权流转情况与思考

矿产资源是国家的重要资源,探矿权、采矿权为国家所有。“两权”的流转应体现公平、竞争的原则,防止国有资产流失。     

冷却水循环用离心泵汽蚀的原因与防止方法探讨

通过对冷却水循环用离心泵产生汽蚀的因素进行分析，提出了防止汽蚀产生的技术措施，经生产应用效果良好。     

杂交条纹鲈苗种小水泥池培育技术试验

用地上露天小水泥池8个．进行杂交条纹鲈苗种培育试验．放养313龄水花30000尾．通过天然饵料、藻粉投喂以及适当水质管理措施．经32天培育，共培育3．5～4．6cm体长的鱼种4336尾．最高成活率为19．1％，最低为6％，平均为14．5％。     

基于Hough变换识别单周期正弦曲线图像的算法及实现

手工识别成像测井中的裂缝具有一定的主观性，不同的人可能会有不同的解释结果．作者介绍利用Hough变换自动识别裂缝的原理及其算法，并通过实例分析了其应用效果，结果表明，这种算法计算裂缝参数不仅精度可靠，还保证了裂缝参数拾取的唯一性，对实现井壁裂缝参数的完全自动解释有重要意义．     

体外循环对血液有形成份的影响（附34例观察）

观察３４例心内直视手术体外循环前后对血液有形成份的影响，发现体外循环后血红蛋白、红细胞与血小板有显著降低，而白细胞显著增高，这可能与机械损伤、血液稀释和机体反应有关。因此选用接近人体生理循环的人工心肺机，改进手术吸引装置、缩短体外循环时间及提高手术质量，可能有助于减少体外循环对血液有形成份的影响。     

标准差在单粒播种机试验中的应用

单粒播种机试验方法中的标准差与众不同,有很多专业特点,研究它利于提高试验水平,进而利于产品质量的提高。为此,探讨了标准差在单粒播种机试验中的应用。     

Quantitative capillary reversed passive latex agglutination test for C-reactive protein (CRP) in the dog

A capillary reversed passive latex agglutination test (capillary RPLA) was developed which allows quantification of serum C-reactive protein (CRP) within approximately 15 min. The logarithmic regression line (calibration curve) obtained after measuring each CRP concentration three times in twofold dilutions of a standard canine serum containing 222 g/ml of CRP was y=6.394+0.030x (r=0.995). Capillary RPLA permitted quantification of CRP in the range 6.9–222 g/ml. The coefficients of variation ranged from 10.28% to 12.40%. The recovery rates (percentage recovery) of CRP by capillary RPLA were within the range 87% to 106%. On measuring the CRP concentrations in sera from 78 dogs by capillary RPLA, single radial immunodiffusion (SRID) and enzyme-linked immunosorbent assay (ELISA), close correlations were demonstrated between SRID and capillary RPLA (y=7.250+1.109x, r=0.978), between SRID and ELISA (y=3.042+1.059x, r=0.967), and between capillary RPLA and ELISA (y=1.778+0.929x, r=0.962). Capillary RPLA may be considered useful as a routine biochemical technique for measurement of serum CRP concentration in the dog.Abbreviations CRP C-reactive protein - ELISA enzyme-linked immunosorbent assay - RPLA reversed passive latex agglutination test - SRID single radial immunodiffusion