Development of Chinese Near-isogenic Line of Rice and Their Differentiating Ability of Pathogenic Races of Blast Fungus

The rice( Oryza L. ) variety, Lijiangxintuanheigu(LTH), is a universally susceptible variety. A set of near-isogenic lines (NILs) of rice which has single resistance gene for each line was developed by use of LTH as recurrent parent and Kusabue,Tsuyuake, K1, PiNo. 4, K60 and BL1, which are from Kiyosawa‘s differential varieties(KDV), as donor parents. Differentiating ability of these NILs to Philippine isolates was compared with that of their donor parents, KDV and IRRI‘ s NILs. The results of comparative studies were as follows: (1) Differentiating ability of Chinese NILs to Philippine isolates was much stronger than that of their donor parents and that of KDV. (2) Chinese NILs had similar ability to differentiate races to IRRI‘s NILs or higher differentiating ability than them. But IRRI‘s NILs almost had no differentiating ability to isolates from japonica rice-growing regions. They can not be used to differentiate races of isolates from japonica ricegrowing regions. Whereas, Chinese NILs can be used to differentiate races of isolates from both japonica ricegrowing regions and indica rice-growing regions. It was concluded that the set of Chinese NILs can be used as international differentials.     

Utilization of Aromatic Rice in Improving Grain Quality of Hybrid Rice

ThesuccessfuldevelopmentandcommercialuseofhybridricehasgreatlyraisedriceyieldlevelinChina .However ,ithasbeenagreatchallengeforbreederstoim provethegrainqualityofhybridricewithoutsacrificeofitsyield ,i.e .,todevelophybridricecombinationswithgoodgrainqualityaswellashighyield .AromaticricelikeBasmatiinIndiaandPakistanandJasmineinThailand ,isfamousforitshighqualityandu niquepleasantflavorofaroma ,soitishighlyfavoredbyriceconsumersaroundtheworld .Itmightbeaneffectiveapproachtoimprovethegrainqual…     

西瓜核型雄性不育两用系POD同工酶研究

用PAGE(聚丙烯酰胺凝胶)电泳技术,研究了西瓜核型雄性不育两用系不同器官的POD(过氧化物酶)同工酶酶谱差异。试验结果表明,不育株与可育株主要差异在雄花蕾,表现为不育株较可育株多2条活性强的酶带;其他器官差异小,仅表现为酶谱带强弱不同。并对POD同工酶与雄性不育性的相关性进行了研究。     

Inhibitory effects of LDL-ACM complex on subcutaneous implanted tumors in nude mice()

AIM: In order to evaluate the applicable value of LDL as a targeted vehicle for chemotherapeutic agents, we investigated and compared the inhibitory effects of LDL-ACM complex and free ACM on nude mice's subcutaneous implanted tumors derived from gastric cancer cell lines, SGC-7901 and NKM-45. METHODS: LDL-ACM complex was prepared and the tumor model of nude mice was established by subcutaneous implantation of SGC-7901 and NKM-45. Then, the groups of nude mice developed subcutaneous implanted tumors were received either LDL-ACM complex or free ACM. Subsequently, the tumor size, weight and leukemia cell counts were measured and the rates of tumor-inhibition and the survival were compared among the groups. RESULTS: The inhibitory effects of LDL-ACM complex on the tumors, especially on SGC-7901 implanted tumors were much more obvious than that of free ACM. It was also indicated that the action of LDL-ACM complex was mediated by LDL receptor. CONCLUSION: These results showed that LDL-ACM complex had significant inhibitory effects on the implanted tumors and the effect might be mediated by LDL receptor.     

黄瓜单体异附加系的筛选与观察

 将甜瓜属异源三倍体与栽培黄瓜‘北京截头’杂交，经胚胎拯救获得了两个2n=15 (14C+1H)的单体异附加系，编号分别为02—17和02—39。02一l7植株叶形为戟形，02—39为深陷的掌状形，并且果刺均为白色，不同于异源三倍体的掌状心脏形和黑刺，果形比异源三倍体黄瓜长而更接近普通黄瓜。细胞学研究发现，单体异附加系在终变期和中期I染色体构型主要是由7个二价体和1个单价体构成，出现三价体的频率为4％ ～5％ ，中期Ⅱ不均等分裂形成不同的两极，一极染色体为8条，另一极为7条。RAPD分析证明它们分刖附加了野生种Cucum／s hystrix的染色体。     

小麦1BL/1RS易位系1RS分子标记位点稳定性分析

为检测小麦1BL／1RS易位系1RS位点的稳定性,利用1RS上11个标记位点的PCR特异引物对21个小麦1BL／1RS易位系进行了分子检测。结果表明,21个1BL／1RS易位系中,66．7％的品种1RS的11个标记位点较为稳定,扩增出标记位点特异性带,但有33．3％的品种部分标记位点出现特异性带的丢失或添加,具有不同程度的位点变异,位点变异率最高达45．5％。对于1RS上的11种PCR特异引物,引物NOR-R1和APR1．3可稳定扩增出黑麦特异带,是在小麦遗传背景中鉴别黑麦1RS较为可靠的分子标记。     

花生抗锈病种质筛选及抗性QTL分析

锈病是影响花生产量和品质的主要真菌性病害。本研究以“中花10号×ICG 12625”构建的重组自交系群体(RIL)为材料,在武昌和阳逻两个环境中对其F₈群体进行锈病抗性鉴定,获得了8份抗锈病材料(QT0348、QT0368、QT0400、QT0402、QT0419、QT0458、QT0463和QT0485)。利用前期构建的遗传图谱进行QTL分析,检测到10个与锈病抗性相关的QTL,贡献率为4.54%～10.78%,分布于7条染色体上。其中,qBB06.1和qBB06.4为重复一致的QTL,贡献率为10.76%。根据侧翼标记在物理图谱进行比对,发现对应区段含有193个基因,其中178个基因被注释,功能注释基因中有1个NBS-R基因。本研究结果为花生抗锈病的遗传改良奠定了基础。     

人血小板生成素基因的克隆及CHO稳定细胞系的建立

 以人肝cDNA为模板克隆了人血小板生成素（Human Thrombopoietin,hTPO）基因,利用基因重组技术构建了带有新霉素抗性基因（neo）筛选标记的pcDNA3.1(+)-hTPO真核表达载体,将重组质粒瞬时转染293T细胞,用鼠抗人TPO 单抗Western blot 检测 TPO蛋白的瞬时表达;再将重组质粒转染中国仓鼠卵巢细胞(Chinese Hamster Ovary,CHO),应用400 μg/mL的G418筛选克隆,经PCR及Western blot验证,获得了3株hTPO蛋白表达水平不同的CHO细胞系,为获得大量蛋白并进行活性功能试验及临床应用奠定基础。     

粘类小麦雄性不育系雌雄不育配子传递率研究

为了给粘类小麦雄性不育系的利用提供理论依据,对偏、粘、易、二角型小麦雄性不育系及其杂种F1雌雄不育配子的传递进行了研究.结果表明,雌雄不育配子传递率均低于50%,粘、易、偏型不育系F2代供试群体全部可育,并且正反交不育配子通过雄配子的传递率都为0,初步认为符合配子体不育的特点;二角型不育系正反交雌雄不育配子传递率也都低于50%,但不育雄配子传递率要高于雌配子传递率,并且正反交都有不育株出现.所以二角型不育系是配子体不育还是孢子体不育尚有待进一步研究.     

几类异质小麦雄性不育系育性恢复的染色体行为研究

为了研究异质小麦雄性不育系减数分裂中期Ⅰ与后期Ⅰ的染色体行为及与育性恢复的相关性,以几类异质小麦雄性不育系为母本,用常规品种(系)作为父本与其杂交,调查了杂种F1减数分裂中期Ⅰ单价体频率与后期Ⅰ染色体变异率及自交结实率之间的相关性.结果表明:(1)花粉母细胞减数分裂中期Ⅰ单价体频率与后期Ⅰ染色体变异率呈显著正相关,后期Ⅰ染色体变异率与自交结实率呈负相关,但不显著;(2)相同异质不育系与相同或不同品种杂交时减数分裂中期Ⅰ单价体频率与后期Ⅰ染色体变异率及结实率差异不显著;(3)不同异质不育系与相同或不同品种杂交时减数分裂中期Ⅰ单价体频率与后期Ⅰ染色体变异率差异性较为显著,但减数分裂中期Ⅰ单价体频率与结实率差异性不显著;(4)异质小麦中,异源胞质影响染色体变异,但核基因是调控的关键因素.