Effect of dasatinib in a xenograft mouse model of canine histiocytic sarcoma and in vitro expression status of its potential target EPHA2

Canine histiocytic sarcoma (HS) is an aggressive and highly metastatic tumor. Previously, the kinase inhibitor dasatinib was shown to have potent growth inhibitory activity against HS cells in vitro, possibly via targeting the EPHA2 receptor. Here, the in vivo effect of dasatinib in HS cells was investigated using a xenograft mouse model. Moreover, the expression status of EPHA2 was examined in six HS cell lines, ranging from insensitive to highly sensitive to dasatinib. In the HS xenograft mouse model, dasatinib significantly suppressed tumor growth, as illustrated by a decrease in mitotic and Ki67 indices and an increase in apoptotic index in tumor tissues. On Western blot analysis, EPHA2 was only weakly detected in all HS cell lines, regardless of sensitivity to dasatinib. Dasatinib likely results in the inhibition of xenograft tumor growth via a mechanism other than targeting EPHA2. The findings of this study suggest that dasatinib is a targeted therapy drug worthy of further exploration for the treatment of canine HS.     

利用SCoT对甜菜种质资源的鉴定及遗传多样性分析

为了研究SCoT分子标记技术对甜菜种质资源鉴定的可行性。利用80条SCoT引物对48份甜菜种质资源进行鉴别,同时对种质资源进行了遗传多样性分析和亲缘关系的鉴定。结果表明,80条SCoT引物中有6条能够扩增出清晰、且多态性高的条带,分别为SCoT1、SCoT12、SCoT13、SCoT14、SCoT17和SCoT23,其中引物SCoT1、SCoT12、SCoT14和SCoT23单独使用均可鉴别全部的48份种质资源,引物SCoT13和SCoT17共同使用可以鉴别48个种质资源;聚类分析结果表明,在遗传距离0.15处,95.8%的种质资源均聚为一类,从分子角度上表明甜菜种质资源遗传距离较小。本研究为利用SCoT分子标记技术鉴别甜菜种质资源、对种质资源进行亲缘关系鉴定、杂交组合亲本选配以及分子标记辅助育种等提供相关科学依据。     

Improvement of Appearance Traits of elite Rice Zhenshan 97B by Molecular Introgression of Targeted about 800 kb Interval from Indica Rice Jiafuzhan Genome

Rice appearance trait is mainly mean grain length (GL), grain width (GW), ratio of length and width (L/W), chalkiness (CH) and transparence (TR). Appearance is the first impression, and rice appearance traits effect significantly the value of commodity. Hybrid rice is relatively low quality, especially on rice appearance quality,which restricts partially its extend and badly export of hybrid seed and rice in China.Shanyou 63, the most famous hybrid rice combination of China, could not be also sustainable application because of its poor qualities. In this studies, We attempt to improve appearance quality of the elite rice maintainer of Zhenshan 97B by using molecular approach of genomic interval targeted introgression. Three F2 populations were constructed to study the classical and molecular genetic of rice appearance traits, at the same time, molecular marker-asisted toimprove the main maintainer line, Zhenshan 97B were performed. There some following results:Three F2 populations were constructed based on crosses of Guangluai 4×Jiafuzhan (GJ popu.), Minghui 86×Jiafuzhan (MJ popu.) and Zhenshan 97B×Jiafuzhan (ZJ popu.). ZJ popu and GJ popu were produced to reconstruct the linkage maps related with rice appearance traits based on BSA, and detect QTL of target traits based on IM.In ZJ popu, 9 QTLs of target traits were detected. The interval, RM169～RM516 on Chromosome 5, was genetic locus of GW, L/W, CH and BW, and the explained variances were 10.9%, 14.9%, 12.0% and 14.2%, respectively.RM214 on chromosome 7, RM339 on chromosome 8 were genetic loci if CH and BW, and the explained variances were 9.4%, 10.0%, 11.0% and 12.1%. besides there is a genetic locus, RM347, of G/W on chromosome 3, and the explained varance was 10.3%. In GJ popu, 5 QTLs of rice appearance traits were detected. The interval, RM169-RM516 on Chromosome 5, were also the genetic loci of GW, CH and BW, and the explained variances were 9.0%, 12.5%, and 13.6%, respectively. RM264～RM80 on chromosome 8 was the genetic loci of GW amd L/W,and the explained variances were 14.7% and 13.4%. The genotypes of all QTLs, except RM214, from Jiafuzhan contributed to improving riee appearance traits.The research was to improve the rice appearance quality of Zhenshan 97B by introgressing QTLs of rice appearance traits from Jiafuzhan, a donor parent, by means of MAS in the process of recurrent backcrossing, 4 SSR markers were used to detect the target genotypes from donor parent, and 70 SSR primers to identify the genetic background of recurrent parent. Consequently, there were 19 target individuals in BC3, in which the mean value of GL, GW, L/W, CH and BW were 6.06±0.04mm, 2.50±0.02mm, 2.43±0.02, 29.5±2.7 and 29.4±2.7%, respectively,and the mean homologous degree (HD) to recurrent parent is up to 98.22%. Compared to Zhenshan 97B, GL, CH and BW decreased 0.17 mm, 34.9% and 34.5% respectively and however L/W increased 0.19. which accounted separately for 25.37%, 54.79%, 54.57% and 10.50% of thedifference of every trait between two parents. It is very luckly to find a individual, 13BC2-3BC3 line, whose GL, GW, G/W, CH and BW were 6.04 mm, 2.43 mm, 2.49,2.5% and 2.5% separately, There is very important signification for it to be used in hybrid rice breeding improving appearance traits. In the research, MAS efficiency of 3 loci (with 4 markers ) was 5.68% and coincident degree was 73.08%.     

玉米不同耐密植品种茎秆穿刺强度的变化特征

以耐密抗倒性不同的品种（稀植大穗品种JK 518和JK 519、耐密抗倒品种XY335和CS 1;中等耐密品种ND 108）为材料,设5个密度处理,研究玉米茎秆的穿刺强度（RPS）的变化特点及其对群体种植密度的响应。结果表明玉米茎秆穿刺强度随生育期递进而增强,不同耐密性品种间随种植密度变化有较大差异。茎秆基部节间的穿刺强度随群体密度增加呈线性递减。茎秆节间RPS随着节位的上升而呈二次函数递减;不同耐密性品种间RPS以抽雄-吐丝期差异明显,以穗位以下节间,尤其第3～6节间表现差异较大。玉米抽雄前茎壁增厚早、     

生态文明视域下欠发达地区精准扶贫效益评价——以四川省南充市为例

[目的]以四川省南充市为例,研究欠发达地区精准扶贫效益,为区域脱贫攻坚决策和社会经济发展提供科学依据。[方法]采用层次分析法(AHP),以南充市精准扶贫项目为研究对象,从经济发展、社会进步、生态保护和自我发展4个维度构建精准扶贫评价指标体系,对2011—2016年南充市精准扶贫效益进行定量评估。[结果]①南充市经济发展效益经历了一个先降低,后升高的过程。其中,2011—2012年是下降期,降幅达20.8%;②南充市社会进步效益呈现下降、上升交替趋势。从2011年的0.585 1降低到2012年的0.404 0,2015—2016年逐渐增加;③南充市生态保护效益逐年上升,但2012—2013年波动下降,且均高于同期经济发展效益和社会进步效益的增加值;④南充市自我发展效益呈现增加、降低交替过程,且2011—2016年,综合效益逐年增加,2013年则波动下降。[结论]南充市自大力实施精准扶贫以来,取得了显著的经济发展效益、社会进步效益、生态保护效益和自我发展效益。     

精准扶贫的逻辑形成基础与研究维度述评

党的十八大以来,学术界围绕精准扶贫理论与实践的研究,成果丰硕。精准扶贫理论来源和其形成背景,构成了精准扶贫的逻辑形成基础。梳理关于精准扶贫的相关研究,可以归纳出精准扶贫的概念界定和内涵特征、实践模式和路径、精准扶贫实施过程中的问题等研究维度。考察学术界现有研究发现,研究中缺乏互动式辩论,实证性研究较少等,针对这些问题进行深化研究有助于在我国扶贫工作的冲刺期构建更有针对性的扶贫机制。     

基于亲水相互作用液相色谱-三重四极杆质谱法研究白茶萎凋过程中代谢物的变化

采用亲水相互作用液相色谱-三重四极杆质谱法,对白茶鲜叶(F)和室内萎凋处理29 h(W)、53 h(P)的2个萎凋样进行了代谢物靶向检测,Quality control(QC)样本中共检测到111个代谢物,主要包括47种氨基酸及其衍生物、29种核酸类代谢物、12种维生素和辅酶、17种糖代谢物和6种甘油磷脂代谢物。与鲜叶F相比,萎凋叶W和P中显著增加的代谢物分别有39种和41种,其中共有代谢物35种;显著降低的代谢物分别有9种和11种,其中共有代谢物8种。显著变化代谢物分析结果表明,萎凋叶的细胞膜受损、DNA和RNA降解、氨基酸衍生物显著增加、糖代谢异常。根据代谢物含量的变化幅度和组成特异性,筛选了表征萎凋叶细胞膜生理状态和核酸、糖代谢的标志代谢物。此外,针对代谢物中氨基酸和核苷酸的组成特点,提出了研究氨基酸衍生物滋味特性的必要性和应用代谢谱分析建立萎凋工艺适度标准的可能性。     

乡村振兴背景下贵州食用菌精准扶贫产业的现状与路径创新

为促进贵州省食用菌基地大幅度进步和食用菌产业的发展,针对贵州省食用菌基地现阶段发展详情,可能存在的问题,并结合国家现阶段发展政策,对贵州省食用菌基地发展提供科学、合理、有效的建议。     

旅游精准扶贫助推贫困地区乡村振兴的思考

贫困地区精准脱贫是乡村振兴的重点难点和优先任务,旅游扶贫已经成为我国贫困地区乡村振兴的重要方式。推动旅游精准扶贫与乡村振兴的有机衔接,是实现贫困地区乡村振兴的有效途径。本文立足文献述评、调研认知与研究思考,探讨旅游精准扶贫在贫困地区乡村振兴中的主要作用、现实问题和推进途径。研究表明,旅游精准扶贫可以促进贫困地区乡村振兴已成为各方共识,同时也面临利益分配机制不合理、内生增长乏力、生态环境制约等诸多挑战,乡村旅游扶贫需精准识别发展乡村旅游所具备的资源、区位、市场等条件和可行性,将乡村振兴战略的思想和原则融入具体的旅游精准扶贫计划与行动之中。具体需要准确把握好适宜区域、实施主体、主导产业、资金来源和保障机制,创新乡村旅游扶贫的商业模式、聚合乡村旅游扶贫的智力资源、夯实乡村旅游扶贫的文化底蕴、坚守乡村旅游扶贫的绿色发展底线、优化乡村旅游扶贫管理和组织机制,进而从产业振兴、人才振兴、文化振兴、生态振兴和组织振兴等五个方面全方位精准有效地助推乡村振兴。