利用SCoT对甜菜种质资源的鉴定及遗传多样性分析

为了研究SCoT分子标记技术对甜菜种质资源鉴定的可行性。利用80条SCoT引物对48份甜菜种质资源进行鉴别,同时对种质资源进行了遗传多样性分析和亲缘关系的鉴定。结果表明,80条SCoT引物中有6条能够扩增出清晰、且多态性高的条带,分别为SCoT1、SCoT12、SCoT13、SCoT14、SCoT17和SCoT23,其中引物SCoT1、SCoT12、SCoT14和SCoT23单独使用均可鉴别全部的48份种质资源,引物SCoT13和SCoT17共同使用可以鉴别48个种质资源;聚类分析结果表明,在遗传距离0.15处,95.8%的种质资源均聚为一类,从分子角度上表明甜菜种质资源遗传距离较小。本研究为利用SCoT分子标记技术鉴别甜菜种质资源、对种质资源进行亲缘关系鉴定、杂交组合亲本选配以及分子标记辅助育种等提供相关科学依据。

Beet Germplasm Resources: Identification and Genetic Diversity Analysis by SCoT

To study the feasibility of SCoT molecular marker for identifying beet germplasm resources, we designed 80 SCoT primers to identify 48 beet germplasm resources, and analyzed the genetic diversity and genetic relationship of the germplasm resources. The results showed that 6 of the 80 SCoT primers, namely SCoT1, SCoT12, SCoT13, SCoT14, SCoT17, and SCoT23, amplified clear and highly polymorphic bands. Among them, SCoT1, SCoT12, SCoT14 and SCoT23 could separately identify the 48 beet germplasm resources. The identification of the 48 beet germplasm resources could be distinguished by combining SCoT13 and SCoT17. The cluster analysis results showed that 95.8% of germplasm resources were clustered into the same clade at the genetic distance of 0.15, which indicated that there was close genetic relationship among the 48 beet germplasm resources. The study provided a scientific basis for the identification of beet germplasm resources, hybrid combination parent selection and molecular marker-assisted breeding by SCoT markers.