伊犁部分地区马泰勒虫抗体的检测

为了解伊犁部分地区马场感染马泰勒虫(Theileria equi)的情况,试验将重组原核表达质粒pGEX4T-1/EMA1转化至大肠杆菌菌株BL21表达系统中,诱导表达56 ku的重组蛋白(EMA1);经KCl方法切胶纯化后作为间接ELISA包被抗原,对伊犁州六个不同地区随机采集的样品(n=352)进行马泰勒虫抗体的检测。结果表明:以GST-EMA1蛋白作为包被抗原建立的间接ELISA法可明显区分马泰勒虫阳性血清和健康马血清。在所检测的血清样品中,马泰勒虫血清抗体阳性率为7.4%(26/352),其中乌尊布拉克乡的马泰勒虫血清抗体阳性率为7.3%(4/55)、昭苏镇的马泰勒虫血清抗体阳性率为13.2%(10/76)、萨尔阔布乡的马泰勒虫血清抗体阳性率为8.2%(6/73)、察汗乌苏乡的马泰勒虫血清抗体阳性率为6.5%(4/62)、喀拉苏乡的马泰勒虫血清抗体阳性率为2.0%(1/49)、喀夏加尔乡的马泰勒虫血清抗体阳性率为2.7%(1/37);伊犁州等六个地区马场马泰勒虫血清抗体阳性率随年龄而改变,其中7岁马匹的马泰勒虫的血清抗体阳性率最高。说明马泰勒虫在伊犁地区马匹中普遍存在,应该加强对该病的检测、监控。     

岔口驿马产区马腺疫和马鼻肺炎血清学调查

为明确岔口驿马产区内马腺疫(equine strangles)和马鼻肺炎(equine rhinopneumonitis, ER)流行情况，保障岔口驿马产业健康稳定发展，特进行血清学调查。按随机采样方式，采集甘肃省天祝县16个乡镇的岔口驿马血清510份，用ELISA方法进行马链球菌(Streptococcus equi,S.equi)和马疱疹病毒(Equine herpesviruse, EHV)抗体检测。结果显示，510份马血清中检测出S.equi抗体阳性血清73份，总阳性率为14.31%;检测出EHV抗体阳性血清66份，总阳性率为12.94%。其中，1岁龄以内马匹S.equi和EHV抗体阳性率分别为1.04%、8.33%,1岁至3岁龄马匹抗体阳性率分别为27.19%、14.04%,3岁至5岁马匹抗体阳性率分别为17.33%、14.67%,5岁以上马匹抗体阳性率分别为10.00%、13.33%,且存在同时检测出S.equi和EHV抗体阳性的情况，混合抗体阳性率为6.27%。岔口驿马产区应针对马腺疫和马鼻肺炎，因地制宜，科学制定防控措施。     

新疆4县马腺疫链球菌血清抗体检测与分析

为了解新疆养马集中县域马腺疫链球菌的感染情况及流行特点,在新疆特克斯县、昭苏县、富蕴县和吉木乃县采集2 549份马血清样品,采用酶联免疫吸附试验(ELISA)检测马血清中的马腺疫链球菌抗体。结果表明,被检样品的马腺疫链球菌抗体平均阳性率为23.93%,昭苏县及特克斯县的血样中马腺疫链球菌抗体阳性率较高,分别为30.30%和26.64%,吉木乃县抗体阳性率为4.34%。结果表明,新疆不同地区马腺疫链球菌血清抗体阳性率差异显著(P0.05),抗体阳性率随马匹年龄的增长不断提高,0~1岁马抗体阳性率最低(P0.01),马腺疫链球菌抗体阳性率性别差异不显著(P0.05)。     

广州市马红球菌病血清学调查

本研究对广州市8家马场及散户马匹随机抽样,采用本研究室建立的ELISA检测方法,对马红球菌病血清学分布情况进行调查,分析各影响因素与其抗体阳性率和量的相关性。结果表明:广州市马匹马红球菌病平均抗体阳性率为58.7%;性别因素与阳性率与抗体量相关,但均不显著;品种因素与马红球菌病抗体阳性率无关,且阳性马匹中,纯血马抗体量高于国产马及其他品种马匹,但无显著性差异;马场环境因素与阳性率及抗体量显著相关;季节因素与马红球菌抗体阳性率及血清阳性抗体量间无明显相关性。本研究通过系统的流行病学方法对马红球菌病进行血清学、流行病学统计调查与分析,不仅为广州市马无疫区建设及我国马红球菌病的防控措施建设提供必要参考,且对该疾病防控具有重要的公共卫生学意义。     

天祝白牦牛弓形虫感染情况调查

为查明天祝县白牦牛弓形虫感染情况,采用间接血凝试验(IHA)对甘肃省天祝藏族自治县6个采样点共498份白牦牛血清进行弓形虫血清学检测。结果显示:87份待检血清呈阳性,阳性率17.47%,以1～2岁年龄段弓形虫抗体阳性率最高。     

广西牛羊赤羽病和蓝舌病流行病学调查

为了解赤羽病病毒(AKAV)和蓝舌病病毒(BTV)2种虫媒病毒(Arbovirus)在广西的流行与分布情况,本研究对采集自2010年～2011年广西11市的706份牛、羊血清样品进行检测,共检测出AKAV抗体阳性样品382份,总阳性率为54.11％.其中山羊血清82份,阳性率为28.37％(82/289);牛血清300份,阳性率为71.94％(300/417).BTV抗体阳性样品279份,总阳性率为39.52％.其中山羊血清95份,阳性率为32.87％(95/289),牛血清样品184份,阳性率44.12％(184/417).同时检测出2种虫媒病毒抗体185份,占样品总数的26.20％.本研究结果表明2种虫媒病毒在广西广泛流行,并首次证明广西存在AKAV的感染.     

2020年湖南及周边地区猪伪狂犬病病毒血清学调查及分析

为初步了解湖南及周边地区猪伪狂犬病免疫及野毒感染情况,于2020年1—12月从湖南及周边地区238个猪场采集血清样品7194份,通过酶联免疫吸附试验(ELISA)分别检测伪狂犬病病毒(PRV)gB抗体和gE抗体.结果发现,667份血清样品为PRV-gE抗体阳性,5937份血清样品为PRV-gB抗体阳性,其平均阳性率分别...     

北疆部分地区马新孢子虫病血清学调查初报

为了解新疆北疆地区马匹感染新孢子虫病的情况,课题组成员运用自制新孢子虫病r ELISA抗体试剂盒,对采自阿勒泰富蕴县4个乡镇的160份马血清和伊犁地区3个马场的270份马血清进行了血清学检测。结果表明:阿勒泰富蕴县4个乡镇的马新孢子虫病阳性率分别为15.00%、13.85%、13.30%、8.00%,其总感染率为13.13%(21/160);伊犁地区3个马场马新孢子虫病阳性率分别为5.68%、9.82%、11.43%,其总感染率为8.89%(24/270)。经SPSS软件统计分析,两个地区之间的感染率差异显著(P0.05)。     

新疆伊犁地区部分马病血清学调查

为了解新疆伊犁地区马群马流感病毒(equine influenza virus)、马疱疹病毒(equine herpesviruses)以及马沙门菌(Salmonellosis equinum)、马链球菌(Streptococcus equi)的感染情况,采用酶联免疫吸附试验及血凝抑制试验对采自新疆伊犁地区7个马场的277份马血清进行抗体水平检测。结果显示:4种病原中,沙门菌血清抗体阳性率最高26.71%(74/277),马疱疹病毒最低15.16%(42/277);各马场之间马流感病毒、马疱疹病毒,以及沙门菌、马链球菌抗体阳性率差异性极显著(P0.01);不同马场之间马沙门菌和马链球菌双抗体阳性率差异性极显著(P0.01);不同马场之间马鼻肺炎和马流感双抗体阳性率差异性极显著(P0.01)。结果表明:新疆伊犁部分马群存在不同程度的马流感病毒、马疱疹病毒、马沙门菌以及马链球菌的感染。此次调查数据可为新疆地区部分马病的防控工作及马产业的健康发展提供参考。     

陕西省部分猪场5种疫病抗体及病原的检测

为调查2019年陕西省规模化猪场中口蹄疫(FMD)、猪繁殖与呼吸综合征(PRRS)、猪瘟(CSF)、猪圆环病毒2型(PCV2)和猪伪狂犬病(PR)的免疫状况及病原感染情况,采用间接ELISA对送检的20 233份血清样品进行抗体检测,采用PCR/RT-PCR方法对送检的1 633份病料样品进行病原检测。结果表明,检测口蹄疫抗体的5 154份血清样品中(4 089份免疫血清,1 065份未免疫血清),免疫抗体阳性率为76.69%,感染率为42.25%;检测猪繁殖与呼吸综合征抗体的6 259份血清样品中(5 516份免疫血清,743份未免疫血清),免疫抗体阳性率为85.06%,感染率为28.26%;检测猪瘟抗体的5 165份血清样品中(4 881份免疫血清,284份未免疫血清),免疫抗体阳性率为88.10%,感染率为47.89%;检测伪狂犬病病毒gB抗体的3 142份血清样品中(2 810份免疫血清,332份未免疫血清),免疫抗体阳性率为84.23%,感染率为21.69%;检测猪圆环病毒2型抗体的513份血清样品中(420份免疫血清,93份未免疫血清),免疫抗体阳性率为95%,感染率为88.17%。检测猪瘟病毒的328份病料中,阳性率为9.45%;检测猪圆环病毒2型的218份病料中,阳性率为14.23%;检测猪繁殖与呼吸综合征病毒的654份病料中,阳性率为10.86%;检测伪狂犬病病毒的144份病料中,阳性率为2.78%;检测口蹄疫病毒的226份病料中,阳性率为0.44%。通过对5种常见病原和抗体的检测,为陕西省猪场常见疫病的防控提供了基础资料。     

Heartworm in dogs in Canada in 1989

In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated.

Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively.

     

Heartworm in dogs in Canada in 1986

In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%).

Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada.

     

Heartworm in dogs in Canada in 1987

In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%).     

Heartworm in dogs in Canada in 1983

In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario.

Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously.

     

Heartworm in dogs in Canada in 1984

In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal.

Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection.

     

Heartworm in dogs in Canada in 1985

In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%).

Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada.

     

Heartworm in dogs in Canada in 1988

In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%).