早期限饲对肉鸡体内脂质过氧化作用和抗氧化酶活性的影响

采用低温诱发肉鸡腹水综合征(AS)，并观察早期限饲对常温和低温饲养时肉鸡AS的发病率、肉鸡体内脂质过氧化作用和抗氧化酶活性的影响。试验1，限饲处理组肉鸡分别于7～14日龄或7～21日龄每天给料8h，对照组肉鸡全程自由采食。试验2，限饲组肉鸡分别于7～14日龄给予对照组肉鸡前一天饲料消耗量的60％或80％，对照组肉鸡自由采食。试验过程中连续测定肉鸡血浆超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH—Px)、丙二醛(MDA)水平和腹水心脏指数(RV／TV值)。结果显示低温显著升高了肉鸡血浆MDA浓度，降低了血浆SOD和GSH—Px的活性；低温饲养肉鸡5～6周龄时的RV／TV值显著升高，AS的发病率显著增加。限饲处理显著降低了肉鸡血浆MDA含量，并且限饲处理肉鸡生长后期血浆中SOD和GSH—Px活性显著高于自由采食组肉鸡。所有的限饲处理均能降低肉鸡AS的发病率，并且早期限饲处理肉鸡42日龄的RV／TV值显著低于非限饲肉鸡。这表明早期限饲能够减轻肉鸡体内脂质过氧化作用，提高机体抗氧化酶的活性，从而降低AS的发病率。     

早期限饲对肉鸡血浆NO水平、红细胞比容和心脏指数的影响

试验测定了常温和低温下限饲肉鸡和非限饲肉鸡体内一氧化氮(NO)含量、红细胞比容(PCV)和心脏指数的变化,以探讨早期限饲降低肉鸡肺动脉高压综合征(PHS)发病率的机理。试验结果显示低温下肉鸡的PCV值、心脏指数和PHS的发病率显著升高,NO水平在低温早期显著降低,然而随着低温时间的延长,低温处理组肉鸡的血浆NO水平反而代偿性升高。早期限饲显著降低了肉鸡生长早期的红细胞压积值,并显著降低了42日龄心脏指数和PHS的发病率。限饲鸡在限饲期间血浆NO水平显著降低,但其他阶段与非限饲鸡差异不显著。这提示早期限饲能够缓解肉鸡肺动脉高压和右心肥大的产生,其机理可能与NO和PCV改变所致的血流动力学变化有关。     

早期限饲对肉鸡肌肉生长及生产性能的长期影响

本研究旨在比较不同生长阶段隔日限饲(早期限饲:1~14日龄限饲,后期限饲:50~63日龄限饲)对肉鸡肌肉生长及相关激素水平的影响,并探讨早期限饲对肉鸡肌肉生长的程序化作用。试验结果:1)早期限饲组整个试验阶段体重均极显著低于对照组,后期限饲组限饲一周后体重显著低于对照组并于63日龄实现完全补偿生长;2)早期限饲组14日龄和63日龄胸肌、腓肠肌重均显著低于对照组;后期限饲组63日龄胸肌、腓肠肌重均显著低于对照组,且二者与早期限饲组均差异不显著;3)早期限饲显著降低了14日龄肉鸡肌肉中DNA、RNA和蛋白含量,63日龄恢复至正常水平,且核酸、蛋白水平在胸肌和腓肠肌内的趋势表现不一致;4)早期限饲组14日龄血清中T3、T4水平均显著低于对照组,63日龄血清T3水平仍显著低于对照组;后期限饲组63日龄血清T3水平显著低于对照组,T4水平与对照组差异不显著。结果表明:1)限饲可显著阻碍肉鸡肌肉的生长;2)不同部位的肌肉对限饲的敏感度不同;3)早期营养因子对肉鸡肌肉的生长具有"程序化"作用,甲状腺激素可能参与这些过程的调节。     

早期限饲对肉仔鸡生产性能的影响

为探明早期限饲对肉用仔鸡生产性能的影响，作９个饲养试验。日限饲量是以饱饲对照组前２４小时饲料消耗量为基础。限饲期为７￣１４日龄（试验１￣５）和８￣１６日龄（试验６￣９）。试验１￣５处理为饱饲对照组前２４小时饲料消耗量的９０、７５、和６０％，试验６￣９处理为饱和对照组前２４小时饲料消耗量的８０和６０％。腹脂沉积未受早期限饲的影响。早期限饲增进了饲料转换效率（料肉比）。１４日龄体重下降８％的肉仔鸡（９     

间歇光照对肉鸡体内脂质过氧化作用和抗氧化酶活性的影响

低温诱发肉鸡肺动脉高压综合征（PHS）,动态观察限制光照对肉鸡PHS发病率及体内脂质过氧化作用的影响。将320羽1日龄肉鸡随机分为4组。常温组按常规饲养,24 h连续光照。3个低温组从14日龄起逐步降温诱发PHS,并于9～30日龄分别在夜间停止光照0、35、h。结果,低温组PHS发病率、右心全心比（RV/TV）、肺厚壁末梢血管百分率（%TWPV）和丙二醛（MDA）水平显著升高,而血浆超氧化物歧化酶（SOD）活性降低。限制光照组SOD活性升高而PHS发病率、RV/TV、TWPV和MDA显著降低。间歇光照组早期生长速度减慢,后期体质量得到补偿性增长,并在44日龄与连续光照组差异不显著。这一结果表明,减轻体内脂质过氧化作用,提高机体抗氧化酶活性并减轻以非肌型肺动脉肌型化为特征的肺血管重构,可能是限制光照使肉鸡PHS发病率降低的机理之一。     

早期数量限饲对肉用仔鸡生产性能、胴体品质的影响

试验研究了早期数量限饲对肉用仔鸡生产性能、胴体品质的影响。以320只1日龄AA+肉用仔鸡为试验动物。设4个处理组,每个处理组4个重复,每个重复20只肉仔鸡。对照组根据AA+商品代肉鸡营养标准配制。各处理肉用仔鸡1～7日龄均为自由采食,8～14日龄对照组、限饲90%组、限饲80%组和限饲70%组分别饲喂自由采食量的100%、90%、80%和70%,15日龄恢复自由采食,以后按常规饲养管理。每周末,以重复为单位全部称重。分别于42、49日龄,每个重复随机抽取4只肉用仔鸡禁食12h、称重、屠宰、测定屠体品质。试验结果表明:在第2周,限饲各组肉仔鸡的体重和日增重分别显著低于对照组(P0.05);在第3周,限饲90%组完成补偿生长,体重与对照组差异不显著(P0.05);在第4周,限饲80%和70%组完成补偿生长,体重与对照组差异不显著(P0.05);5、6、7周及2～6、2～7周各组在体重、日增重、日采食量和饲料转化率等都无显著差异(P0.05)。早期数量限饲对肉仔鸡胴体特性没有显著影响(P0.05),限饲70%～80%,具有改善饲料转化效率、胸肌率、腹脂率的作用。     

早期限饲对肉鸡补偿生长的影响

试验旨在研究不同时间、不同限饲水平对肉鸡补偿生长的影响。试验选取0日龄健康状况良好的艾维茵雏鸡330只,随机分为4个试验处理组,除对照组外,其余3个试验处理组各设三个限饲水平,每组3个重复(对照组为6个重复),每个重复10只鸡。结果表明:11～17日龄限饲最好,其最佳限饲水平为47%(R~2=0.9051);5～11、8～14日龄最佳限饲水平为14%;超过29%时,49日龄不能获得完全补偿生长(R~2=0.5913);限饲改善饲料利用率,随着限饲水平提高而提高(R~2=0.966),30%的限饲水平显著提高饲料利用率(P0.05)。结论:5～11、8～14日龄最佳限饲水平为14%;限饲可降低肉鸡总死亡率。     

早期限饲对肉鸡补偿性生长的影响

试验观察了几种早期限饲措施对肉鸡补偿性生长和消化器官发育的影响。结果显示,各种早期限饲处理均可明显降低肉鸡早期的生长速度和体重;恢复自由采食后肉鸡获得了补偿性生长,适当的早期限饲并不影响肉鸡的最终上市体重;早期限饲改善了饲料效率;限饲鸡胰脏相对重量和肌胃 腺胃相对重量显著高于非限饲鸡,提示早期限饲诱发肉鸡补偿性生长可能与限饲促进消化器官发育、提高消化能力有关。     

早期限饲对肉鸡心肌易颤性和血清心肌酶活性、电解质水平的影响

艾维因商品肉鸡于 4～ 12日龄时进行 10 %限饲 ,分别于 2 5、32日龄进行心肌易颤性的评价和血清心肌酶活性、血清电解质水平的检测。结果如下 :(1)早期限饲可以提高肉鸡的输钾诱颤阈 ,表明可降低肉鸡的心肌易颤性 ;(2 )早期限饲可以降低血清心肌酶 (AST、L DH、CK)的活性 ,表明可增强肉鸡的心肌机能 ;(3)早期限饲对血清电解质水平无明显影响。本试验验为进一步阐明早期限饲降低肉鸡猝死综合征发生的机制提供了理论依据     

数量限饲对肉用仔鸡生产性能胴体品质的影响

试验:早期数量限饲对肉用仔鸡生产性能、胴体品质的影响以15只1日龄艾维茵肉用仔鸡为试验动物。饲养时间为0~7周龄。试验设2个处理组,一组为数量限制组为自由采食组的85%,另一组为对照组,即自由采食组。结果:限饲组的日增重、饲料转化率、4~6周龄体重均显著低于自由采食组(P0.05);限饲组可以提高肉鸡肝脏指数。早期数量限饲有降低肉鸡全期平均日采食量、日增重及料肉比的趋势;早期数量限饲降低肉鸡4~6周龄的平均日采食量(P0.01)及显著降低日增重(P0.05)。     

Effects of early feed restriction and cold temperature on lipid peroxidation, pulmonary vascular remodelling and ascites morbidity in broilers under normal and cold temperature

Two experiments were conducted to evaluate the effects of early feed restriction on lipid peroxidation, pulmonary vascular remodelling and ascites incidence in broilers under normal and low ambient temperature. In experiment 1, the restricted birds were fed 8h per day either from 7 to 14 d or from 7 to 21 d, while the controlled birds were fed ad libitum. In experiment 2, the restricted birds were fed 80 or 60% of the previous 24-h feed consumption of full-fed controls for 7 d from 7 to 14 d. On d 14, half of the birds in each treatment both in experiment 1 and experiment 2 were exposed to low ambient temperature to induce ascites. Body weight and feed conversion ratio were measured weekly. The incidences of ascites and other disease were recorded to determine ascites morbidity and total mortality. Blood samples were taken on d 14, 21, 28, 35 and 42 to measure the plasma malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). On d 42, samples were taken to determine the right/total ventricular weight ratio (RV/TV), vessel wall area/vessel total area ratio (WA/TA) and mean media thickness in pulmonary arterioles (mMTPA). Low-temperature treatment increased plasma MDA concentration. When broilers were exposed to a cool environment for 3 weeks, plasma SOD and GSH-Px activity were decreased compared with normal-temperature chicks. RV/TV, WA/TA and mMTPA on d 42 were increased in birds exposed to cold, consistent with the increased pulmonary hypertension and ascites morbidity. Early feed restriction markedly decreased plasma MDA concentration. The plasma SOD and GSH-Px activity of feed-restricted birds were markedly higher than those fed ad libitum on d 35 and d 42. All early feed restriction treatments reduced ascites morbidity and total mortality. On d 42, the RV/TV, WA/TA and mMTPA of feed-restricted broilers were lower than that of the ad libitum-fed broilers. The results suggested that early feed restriction alleviated the lipid peroxidation, promoted the activity of enzymatic antioxidant and inhibited pulmonary vascular remodelling. These changes might be associated with reduced ascites incidence.     

早期数量限饲对肉仔鸡生长性能和胴体特性的影响

试验研究了早期数量限饲对肉仔鸡生长性能和胴体品质的影响。200只8日龄艾维茵肉仔鸡被随机分成4组,每组五个重复,每重复10只鸡。对照组基础饲粮根据NRC营养水平配制,试验组肉仔鸡从8日龄开始喂料量分别为对照组的90%、80%和70%,限饲1周后各组恢复自由采食。试验结果表明:在第2周,限饲各组肉仔鸡的体重和日增重分别显著低于对照组(P<0.05);在第3周,限饲90%组完成补偿生长,体重与对照组差异不显著(P>0.05);在第4周,限饲80%和70%组完成补偿生长,体重与对照组差异不显著(P>0.05);5、6、7周及2～7周各组在体重、日增重、日采食量和饲料转化率等方面都无显著差异(P>0.05)。早期数量限饲对肉仔鸡胴体特性没有显著影响(P>0.05)。     

早期限饲对肉鸡免疫器官发育和体液免疫功能的影响

对肉鸡采用几种不同的早期限制饲喂处理，对比观察了不同的限饲方法对内鸡的抗病力和免疫相关指标的影响。试验结果显示，早期限饲显著降低了肉鸡的发病率和死亡率，这可能与早期限饲促进了肉鸡的脾脏、法氏囊、胸腺等免疫器官的发育，提高了机体的抗体滴度有关。早期限饲对内鸡的上市体质量和饲料转化率未产生显著影响。     

Dietary conjugated linoleic acids alleviate early inflammatory response caused by lipopolysaccharide injection in male broiler chicks

An experiment was undertaken to determine if dietary conjugated linoleic acid (CLA) influences the early stage of the inflammatory response caused by a single injection of Salmonella enteritidisi lipopolysaccharide (LPS) in male broiler chicks. Chicks (7 days old) were fed either 0 or 10 g CLA/kg in their diet for 21 days. At 28 days old, birds were injected intraperitoneally with 1.5 mg LPS/kg body weight after 12 h fasting. Changes in feed intake, rectal temperature and plasma α‐1 acid glycoprotein and ceruloplasmin concentrations were measured during 24 h after LPS injection. Chicks fed the CLA diet were fed more feed for 24 h, had a lower rectal temperature at 9 and 24 h and a lower plasma α‐1 acid glycoprotein concentration at 24 h after LPS injection than chicks fed the basal diet. Plasma ceruloplasmin concentration tended to be lower in chicks fed the CLA diet than in chicks fed the basal diet. The results suggest that dietary CLA alleviate undesirable early inflammatory response due to LPS injection in male broiler chicks.     

The effect of dietary l-carnitine supplementation on pulmonary hypertension syndrome mortality in broilers exposed to low temperatures

Oxidative stress is involved in the development of pulmonary hypertension syndrome (PHS) in broilers. l-Carnitine has an antiperoxidative effect and supplemental l-carnitine has been revealed to increase broiler heart weight. The present study was conducted to evaluate the effect of an addition of 100 mg/kg l-carnitine to the basal diets on PHS mortality in cold-exposed broilers. Two-hundred and forty mixed-sex broilers were equally assigned to three groups. The control group was reared in normal temperatures throughout the experiment. Starting on day 14 continuing until the end of the experiment, the other two groups were subjected to a step-down temperature programme (by lowering the temperature 1-2 degrees C per day down to 12-14 degrees C) with or without l-carnitine added to the basal diets. Cold exposure increased the right/total ventricle ratio (RV/TV) and plasma malondialdehyde (MDA), reduced superoxide dismutase (SOD) and led to pulmonary vascular remodelling in birds without feeding additional l-carnitine. Supplemental l-carnitine reduced plasma MDA, increased SOD, inhibited remodelling and postponed the occurrence of PHS for 1 week in cold-exposed broilers; nevertheless, it did not significantly influence the cumulative PHS mortality (p > 0.05). On days 24 and 32, birds fed supplemental l-carnitine had lower RV/TV and higher total ventricle/body weight (p < 0.05) but unchanged right ventricle/body weight ratios (p > 0.05) compared to their cold-exposed counterparts, indicating an increase in left ventricle weight. However, from day 39 on, their RV/TV ratios were suddenly increased (p < 0.05). It was suggested that the l-carnitine-induced increase in left heart weight might partially account for the postponed occurrence of pulmonary hypertension in the early stage by elevating cardiac output, which might, in turn, lead to the resulting increase in pulmonary pressure. In view of its complex effects on cardiopulmonary haemodynamics, l-carnitine supplementation may be impractical for reducing PHS.     

限饲对商品代AA肉仔鸡生产性能及其经济效益的影响

本研究通过对蛋白质、能量和饲料采食量的限饲,探讨商品代AA肉仔鸡(arber acres)在35日龄出栏时,限饲对生产性能及其经济效益的影响。试验选用了1日龄AA健康肉仔鸡960只,研究在7~14日龄限饲对35日龄出栏时的生产性能和经济效益的影响。研究结果表明,饲料采食量以限采食量组最低,而体增重、料重比和死淘率最佳值都出现在限采食量组,限蛋白质组的效果最差。经济效益分析结果也表明,限饲料采食量组的效益最好。上述研究结果表明,肉仔鸡在7~14日龄时限制采食量25%可提高其生长、生产性能和经济效益。因此,在实践生产中可以考虑予以推广采用。