猪捷申病的防控

猪捷申病由小RNA病毒科捷申病毒属中的猪捷申病毒（PTV）所致,主要引起猪脑脊髓炎、母猪繁殖障碍、肺炎、下痢、心包炎和心肌炎。1929年Trefny氏最先于原捷克斯洛伐克的捷申地区发现该病,之后,西欧、北美、澳大利亚和亚洲均有报道,目前已遍布亚洲。     

猪捷申病毒的诊断方法

猪捷申病（teschendiseaes）最初发现于捷克的捷申地区，因此而得名，是一种急性的致死性猪传染性疾病，能引起严重的中枢神经系统紊乱，又命名为捷申病毒脑脊髓炎（teschovirusencephalomyelifis），该病是Teschen毒株感染引起，属于猪捷申病毒1型。此病症状呈现多样性：神经系统紊乱、繁殖障碍、肺炎、鼻炎、腹泻、心包炎和心肌炎以及皮肤损伤等。随着猪捷申病的流行，猪捷申病毒强毒逐渐被弱毒取代，临床表现上开始以隐性感染为主，但常与猪的其他疾病混合感染，造成猪的发病甚至死亡。     

猪捷申病的防控

猪捷申病由小RNA病毒科捷申病毒属中的猪捷申病毒(PTV)所致,主要引起猪脑脊髓炎、母猪繁殖障碍、肺炎、下痢、心包炎和心肌炎.1929年Trefny氏最先于原捷克斯洛伐克的捷申地区发现该病,之后,西欧、北美、澳大利亚和亚洲均有报道,目前已遍布亚洲.     

猪捷申病毒的诊断方法

猪捷申病(teschen diseaes)最初发现于捷克的捷申地区,因此而得名,是一种急性的致死性猪传染性疾病,能引起严重的中枢神经系统紊乱,又命名为捷申病毒脑脊髓炎(teschovirus encephalomyelitis),该病是Teschen毒株感染引起,属于猪捷申病毒1型.此病症状呈现多样性:神经系统紊乱、繁殖障碍、肺炎、鼻炎、腹泻、心包炎和心肌炎以及皮肤损伤等.随着猪捷申病的流行,猪捷申病毒强毒逐渐被弱毒取代,临床表现上开始以隐性感染为主,但常与猪的其他疾病混合感染,造成猪的发病甚至死亡. 1临床诊断     

国内猪捷申病病毒研究进展

猪捷申病病毒(Porcine teschovirus,PTV)属于小RNA病毒科(Picornaviridae)捷申病毒属(Teschovirus)成员,主要引起猪的脑脊髓灰质炎、生殖障碍、肺炎、下痢、心包炎和心肌炎等临床症状.2003年首次在我国被发现,引起了猪病研究人员的广泛关注.本文综述了近几年国内对猪捷申病毒的研究进展,以期为猪捷申病毒研究者提供参考.     

猪捷申病概述

猪捷申病是由猪捷申病毒(porcine teschovirus,PTV)引起的猪脑脊髓灰质炎、繁殖障碍、肺炎、下痢、心包炎和心肌炎、皮肤损伤及无症状等多种表现的病毒性传染病。该病于1929年首次在捷克捷申城     

猪捷申病毒的感染及防制

猪捷申病由小RNA病毒科捷申病毒属中的猪捷申病毒所致,主要引起猪脑脊髓炎、母猪繁殖障碍、肺炎、下痢、心包炎和心肌炎。虽然1929年Tre-fny氏最先发现于原捷克斯洛伐克的捷申地区,但是捷申病毒已遍布全国,在大部分猪场均有感染,给养猪业带来巨大损失。1病原猪捷申病毒原属于小RNA病毒科肠道病毒属,1999年病毒分类学委员会将肠道病毒属猪肠道病毒中的11个血清型列为捷申病毒属。捷申病毒粒子由蛋白外壳和内部核心组成,呈球形,直径25～30nm,无囊膜,其外层包裹核衣壳,衣壳由32个壳粒组成,呈20面体对称,无脂蛋白。病毒衣壳由4种结构蛋白即V…     

猪捷申病的鉴别诊断与防治

猪捷申病又称为塔番病、猪脑脊髓灰质炎、猪病毒性脑脊髓炎,它是由猪捷申病毒(PTV)引起的猪脑脊髓灰质炎、繁殖障碍、肺炎、下痢、心包炎和心肌炎、皮肤损伤及无症状等多种表现的一种病毒性传染病。1929年首次暴发于原捷克斯洛伐克的捷申小镇上,当时命名为猪捷申病,随后的20世纪50年代,该病蔓延整个欧洲,当时发病的主要症状为脊髓灰质炎、神经系统紊乱,死亡率高(90%以上),给     

猪捷申病的流行、诊断与防治

猪捷申病是由小RNA病毒科猪捷申病毒(porcine teschovirus,PTV)引起的一种病毒性传染病,是一种危害猪只健康的重要疫病,其临床症状表现为脑脊髓灰质炎、繁殖障碍、肺炎、下痢、心包炎和心肌炎以及皮肤损伤。该病自2003年在我国内蒙古被首次发现以来,近几年在黑龙江、云南、江西和广东等地养猪场相继被发现,表明该病在我国呈流行扩大之势。本文结合近几年国内学者的研究综述了该病的流行、诊断和防治策略,为广大兽医工作者和养猪从业者提供参考。     

猪捷申病毒的感染、流行及防制

<正>猪捷申病由小RNA病毒科捷申病毒属中的猪捷申病毒(PTV)所致,主要引起猪脑脊髓炎、母猪繁殖障碍、肺炎、下痢、心包炎和心肌炎。目前在我国主要和猪繁殖与呼吸综合症病毒     

藏猪源捷申病毒的检测和遗传演化分析

旨在调查四川地区藏猪捷申病毒（porcine teschovirus,PTV）的流行及分子特征和遗传演化。用RT-PCR法对2017-2018年采自四川省甘孜藏族自治州康定市14个藏猪场96份粪便样品进行检测,并对全基因组进行序列分析和基因分型。PCR检测结果表明,PTV核酸阳性率为20.8%（20/96,95% CI=13.2%~30.3%）,14个规模藏猪场中PTV猪场阳性率为57.1%（8/14,95% CI=28.9%~82.3%）,并从2份阳性样本中获得了2条近似全长的PTV全基因组序列,分别命名为SWU-E5-2018和SWU-ZG2-2018,核苷酸序列相似性为83.1%。通过VP1序列分析发现2条序列的血清型分别为3型和6型。通过基因重组分析发现SWU-E5-2018存在重组现象,重组区域位于衣壳蛋白基因P1的552-3 082 nt处。为了进一步研究2条PTV序列的演化过程,以贝叶斯进化分析软件进行分歧时间估算,结果表明,SWU-E5-2018的分歧时间约为2010年,SWU-ZG2-2018的分歧时间约为2011年。本研究首次对藏猪源PTV进行了初步调查,结果表明藏猪中存在一定程度的PTV感染,为深入研究藏猪源PTV遗传变异及其生物学特性提供了参考依据。     

The survey of porcine teschoviruses, porcine circovirus and porcine transmissible gastroenteritis virus infecting piglets in clinical specimens in China

A multiplex PCR assay was developed and evaluated for its ability to simultaneously detect three viral infections of swine. Specific primers were carefully selected from articles published for each of the following three viruses: porcine circovirus type II (PCV2), porcine teschovirus (PTV) and porcine transmissible gastroenteritis virus (TGEV). Each target produced a specific amplicon with a size of 353 bp (PCV2), 168 bp (PTV) and 499 bp (TGEV). The sensitivity of the multiplex PCR using purified plasmid constructs containing the specific viral target fragments was 6.60?×?10², 8.43?×?10² and 7.30?×?10² copies for PCV2, PTV and TGEV, respectively. Among 127 samples which were collected from Heilongjiang, Jilin, Henan and Guangxi provinces, the single infection of PCV2, PTV and TGEV was 99.21, 46.88 and 65.35 %, respectively, and co-infection of the three viruses was 26.77 %. In conclusion, the multiplex PCR has the potential to be useful for routine molecular diagnosis and epidemiology.     

Immunohistochemical detection of porcine teschovirus antigen in the formalin-fixed paraffin-embedded specimens from pigs experimentally infected with porcine teschovirus

Porcine teschovirus (PTV) antigens were detected by a streptavidin-biotin complex method in formalin-fixed paraffin-embedded tissues of 3-week-old pigs that had been inoculated intravenously with PTV Talfan strain. PTV antigens were detected in cytoplasm of nerve cells, glial cells and endothelial cells in the cerebellar nuclei, the grey matter of the midbrain, pons and medulla oblongata and the ventral horn of the spinal cord and of ganglion cells in the spinal ganglion corresponding to those lesions characterized as non-suppurative encephalomyelitis and ganglionitis. The results of this study suggest that nerve cells of the brain stem and spinal cord and ganglion cells of the spinal ganglion permit PTV replication and represent the main target cell population of PTV. This is the first study to demonstrate PTV antigen by immunohistochemistry in formalin-fixed paraffin-embedded tissue specimens from pigs infected with PTV.     

猪捷申病毒HB株的分离与全基因序列分析

为分析河北省某规模化猪场猪捷申病毒(PTV)的遗传变异和进化关系,作者成功分离到PTV HB株,对其进行了毒力测定和动物回归试验,设计引物进行了全基因序列测定,并与国内外42株PTV全基因序列进行了同源性比较。结果表明猪捷申病毒HB株病毒滴度为10-6.4 TCID50.mL-1,健康仔猪接毒28d后均出现明显猪捷申病症状,该毒株全基因组序列长度为7 090bp。系统进化树分析表明,猪捷申病毒HB株(JQ664746)属于PTV-8型血清型,和国内分离的PTV-jilin/2003株(GQ293092)同源性最高,从而为进一步研究该毒株的遗传变异提供参考。     

猪捷申病毒Swine/CH/IMH/03株免疫原性的研究

为确定猪捷申病毒(Porcine Teschovirus,PTV)Swine/CH/IMH/03株免疫原性,本研究将该PTV分离株灭活后分别与氢氧化铝佐剂和Montanide ISA 50V2佐剂混合,制成油佐剂疫苗,并采用不同免疫程序接种实验猪后对病毒抗体进行检测。抗体检测结果表明:PTV Swine/CH/IMH/03分离株能够诱导实验动物产生较高抗体水平,并显著高于对照组;与氢氧化铝佐剂混合制成的佐剂疫苗免疫效果优于Montanide ISA 50V2佐剂;而且一次免疫组与二次免疫组没有明显差异。通过PTV Swine/CH/IMH/03株免疫原性的研究,为后续猪捷申病的预防工作提供技术支持。     

A piglet with concurrent polioencephalomyelitis due to porcine teschovirus and postweaning multisystemic wasting syndrome

A piglet developed respiratory distress followed by difficulty in standing and unsteady gait. The lesions were characterized by polioencephalomyelitis with the predominant distribution in the brain stem, as well as lymphocyte depletion and histiocyte infiltration with cytoplasmic inclusion bodies in the lymphoid tissues throughout the body and interstitial pneumonia. Porcine teschovirus (PTV) antigens were found in the former lesions and porcine circovirus 2 (PCV2) in the latter two lesions. PTV genes were detected from the diencephalon. The results suggest that the piglet was concurrently affected with polioencephalomyelitis due to PTV and postweaning multisystemic wasting syndrome (PMWS) associated with PCV2. They also suggest that the immunosuppressive condition developing in PMWS may have facilitated the infection of the brain with PTV.     

Evaluation Of An Additive Solution For Preservation Of Canine Red Blood Cells

The effect of an additive preservative solution on canine red blood cell posttransfusion viability (PTV) and on selected canine red blood cell biochemical parameters was studied. One unit (450 mL) of blood was collected from 6 clinically normal dogs into the anticoagulant citrate phosphate dextrose, centrifuged, and the plasma removed. The red blood cells were then suspended in 100 mL of a saline, adenine, dextrose, and mannitol solution and stored at 4°C. Aliquots were removed for study at 1, 10, 20, 30, 37, and 44 days. The 24-hour PTV of autologous red blood cells was determined using a sodium chromate (⁶¹Cr) label. Red blood cell concentrations of 2,3-diphosphoglycerate (2,3-DPG), adenosine-5'-triphosphate (ATP), and pH were also determined. Canine red blood cell PTV, pH, ATP, and 2,3-DPG concentrations decreased during storage ( P < .05). The PTV decreased from 94% using day 1 red blood cells to 80% and 75% using day 37 and day 44 red blood cells, respectively ( P < .05). Although the mean PTV of the day 44 stored units equaled the Food and Drug Administration (FDA) minimum standard for human red blood cells, the PTV was substandard in 75% of the day 44 units. The FDA standard was exceeded in 83% of the day 37 units. It was concluded that 37-day-old canine red blood cells preserved with a saline, adenine, dextrose, and mannitol solution are of acceptable quality for transfusion.     

Immunohistochemical distribution of viral antigens in pigs naturally infected with porcine teschovirus

A distribution of porcine teschovirus (PTV) antigens in pigs naturally infected with PTV is presented using the method of immunohistochemical examination. In the nervous system, PTV antigens were found in the cytoplasm of neuronal cells and glial cells distributed in the spinal ventral horn and brain stem, and also in the cytoplasm of ganglion cells in the spinal ganglion. No antigens were seen in the cerebral hemisphere. In the nervous system, the distribution of PTV antigens was consistent with lesions characteristic of nonsuppurative encephalomyelitis. In the other examined organ, PTV antigens were observed in bronchiolar epithelial cells in the lung, hepatocytes in the liver, epithelial cells in the tonsils and the myenteric nerve plexus in the small and large intestine.