豆科牧草研究进展

豆科牧草的属间、属内、种间与种内均存在着巨大的遗传变异，利用这种变异，通过育种与选择，已培育出可良好地适应广泛的环境与管理条件的品种，遗传操作为此提供了更为广阔的空间，在豆科牧草固氮方面，对固氮过程、氮向伴生禾草的传送途径及残余氮对后继农作物的价值均有了进一步的理解。对包括施氮、采摘策略和影响混播草地中禾本科－豆科牧草平衡的胁迫等相互作用因素均已给予了阐明。在禾草／白三叶草草地为基础的家畜生产系统中，氮素的损失及其对环境产生的不良作用低于大量施氮的禾草草地系统。与禾草相比，豆科牧草无是用作放牧、青贮或用作干草，其物理、化学和解剖学等方面的特性均可增加家畜的嗜食性和自由采食量。由于豆科牧草的营养价值、可利用营养成分和采食特征，在单一豆科牧草或豆科牧草比重较大的禾草－豆科牧草混播草地上采食的不同种类、不同类型家畜的个体生产性能均优于在禾草草地上采食者。值得注意的是浓缩单宁在动物营养中的有益作用，这类单宁存在于诸如百脉根（Lotus corniculatus）、红豆草（Onobrychis viciifolia）和冠状岩黄芪（Hedys-crum coroharium）等植物中，其防止家畜患膨胀病，通过使较多的氨态氮到达小肠而提高氮的利用，并减轻绵羊肠道中线虫的作用。显然，培育含浓缩单宁的转基因豆科牧草是植物育种者现阶段的主要目标之一。此外，豆科牧草亦有许多其他用途，例如，在葡萄园中用作保护性植被，禾谷类作物的下层植被和为制药业提供次生化合物等。豆科牧草以其植物多态性和花朵的颜色等亦在美化景观和自然保护区中发挥着作用。可以预见，豆科牧草的作用在温带可持续农业中的作用将会增加，充分发掘现有的知识和相关研究成果，及有效的技术将是未来豆科牧草利用的主要特色。     

Responses of yearling steers to different stocking rates on a subtropical grass-legume pasture

Despite potential benefits, limitations of individual tropical legumes have restricted development of sustainable grass-legume pastures in tropical and subtropical regions. Sowing mixtures of complementary legumes may overcome limitations of individual species. Responses of yearling steers grazing a mixture of three tropical legumes with bahiagrass (Paspalum notatum Flugge) were evaluated at three stocking rates under continuous grazing. Carpon desmodium (Desmodium heterocarpon [L.] DC.), which is persistent under grazing but often difficult to establish, was combined with the short-lived legumes aeschynomene (Aeschynomene americana L.) and phasey bean (Macroptilium lathyroides [L.] Urb.). Diet composition, as determined by microhistological analysis of fecal samples, and animal performance were evaluated in three grazing periods: summer 1987 and spring 1988 (2.0, 3.5, and 5.0 steers/ha) and summer 1988 (3.0, 5.3, and 7.5 steers/ha). Stocking rate did not affect percentage of the selectively grazed legumes, aeschynomene and phasey bean, in the diet. Average daily gain decreased linearly (P less than .05) with increased stocking rate, as is typical for grass pastures. Aeschynomene and phasey bean contributed to diets during the first summer, and carpon desmodium contribution was greater in the second summer. These results indicate that this pasture mixture can provide legume herbage from aeschynomene and phasey bean in the year of sowing and from carpon desmodium thereafter. Over the range of grazing pressures obtained, legume responses were generally consistent; thus, optimizing stocking rate for gain per hectare or for economic returns can be targeted without additional constraints to maintain the contribution of these legumes to grazing livestock.     

闽引2号圆叶决明的适应性研究

为了筛选出更优质的亚热带豆科牧草,从澳大利亚牧草种质资源中心(ATFGRC)引进的41个决明属牧草中筛选出闽引2号圆叶决明(Chamaecrista rotundifolia‘Minyin 2’,原品系号ATF3248),并于2011年通过全国草品种审定委员会审定。1997-2010年进行品种比较试验、区域试验和生产试验,结果表明:闽引2号圆叶决明系多年生直立型热带豆科牧草,干草产量达12000～15000kg.hm-2,比对照品种闽引圆叶决明(C.rotundifolia‘Mi-nyin’)增产27.7%以上,种子产量200～400kg.hm-2,比对照品种增产25%以上(P<0.05),盛花期植株含粗蛋白质16.9%,适宜在我国热带、亚热带红壤山地种植。     

植物生长调节剂在牧草种子生产中的应用

优质的牧草种子是草业与畜牧业健康发展的物资保障。植物生长调节剂的推广使用为提高牧草种子产量开辟了新的途径。依据其作用效果,植物生长调节剂可以分为植物生长促进剂与植物生长抑制剂两大类,目前以后者在牧草种子生产中的应用最为广泛。本文结合国内外研究进展,就两大类植物生长调节剂在牧草种子生产中的应用与作用机理进行了总结,重点论述了其对种子产量以及产量构成因素的影响,并总结了目前研究中存在的问题,提出了相应的研究展望。     

Comparative utilization of warm- and cool-season forages by cattle, sheep and goats

The quality of different classes of forage hay (C3, C4 grasses and legumes) was determined in intake and digestibility trials with mature cattle, sheep and goats. For all nine hays, DM and NDF digestibility by cattle and goats was higher (P less than .05) than by sheep, with no differences due to forage class. Cattle had a higher (P less than .01) DM intake than sheep or goats averaged across forage (92.6 vs 65.8 and 68.6 g/kg BW.75); hay intake was highest on legume, with no difference between C3 and C4 grasses. Mean NDF intake by cattle was greater than by sheep or goats (58.7 vs 39.6 and 42.6 g/kg BW.75); NDF intake for all animal species decreased in the order C4 grass greater than C3 grass greater than legume. Particle passage rates did not differ (P greater than .05) with forage class but were higher (P less than .02) for sheep and goats than for cattle. Prefeeding ruminal DM fill values, determined by emptying, were 10.6, 15.0 and 19.9 g/kg BW1.0 for alfalfa, orchardgrass and switchgrass hays fed to cattle, and 11.2, 11.3 and 16.5 g/kg BW1.0 for the same hays fed to sheep. Estimated turnover times for DM and NDF were shorter (P less than .05) for sheep than for cattle; DM turnover was longer for switchgrass than for alfalfa and orchardgrass, with no forage differences in NDF turnover between these two animal species. Results show that goats were superior to sheep in NDF digestion.     

几种豆禾牧草混播初期生长互作效应的研究

选用豆科与禾本科牧草各两种互为混播组合，研究其混播状态下种子萌发及幼苗生长的互作效应，结果表明：当以白三叶、紫花苜蓿为作用牧草，并随期播量增加时，受体牧草高羊茅与紫羊茅种子萌发及幼轩生长受到显著影响，受体牧草紫花苜蓿种子萌发及幼苗生长则对幼苗生长有促进作用。互作效应强度的进一步分析表明，豆科牧草对禾本科牧草的作用一般较强，并在初期生长阶段有较好的表现。这一结果与混播草地豆科牧草所表现的特征相符。因此本研究可从一个侧面为混播草地豆禾组合相容性提供可资借鉴的高效简捷的判定方法。     

硝酸稀土在优良牧草生产中的应用研究

稀土是牧草生产中一种重要的生长促进剂,在一定的有效浓度范围内对种子萌发、植株生长以及豆科牧草根瘤发育有明显的促进效果。通过以硝酸稀土(纯度为38％)为试验材料的研究表明,在河南省自然气候条件下,对禾草有效的稀土浓度为500 mg/kg,平均增产幅度为8.5％;对豆科牧草有效的稀土浓度为500-600mg/kg,平均增产幅度为8.8％,施用方法以浸种法最佳。     

混播草地中豆科/禾本科牧草氮转移机理及其影响因素

混播草地中豆科牧草与禾本科牧草(简称豆/禾牧草)之间的氮转移在草地农业系统氮循环中具有重要作用。在豆科/禾本科牧草混播系统和豆科/禾谷类作物间作系统存在一种氮素共享的通道,即在间(混)作中,豆科植物固定大气中的氮在满足自身生长需求前提下,还通过各种途径为伴生的禾本科植物提供氮源。在混播草地中氮素转移途径主要分地上和地下两种。地上途径主要是豆科牧草的地上部分经放牧家畜采食后粪便归还土壤,后又被禾本科牧草吸收利用或者地上凋落物在土壤中经微生物分解矿化释放出有效氮被另一种植物吸收利用(反之亦成立)。地下途径相对复杂,可能有以下3种:1)通过植物根际沉积氮转移。2)通过菌根真菌的菌丝传递。3)通过植物根系分泌物中含氮化合物来转移。目前的研究虽然明确了有可能转移的途径,但哪一种途径是主要的方式?在氮素转移的过程中,某一途径会部分的发生,还是好几种途径同时发生,每一个途径的贡献为多少?这仍缺少关键的证据。本研究针对国内外关于豆/禾混播草地中豆科牧草生物固氮、豆/禾牧草间氮转移的研究现状,重点对混播草地中豆/禾牧草之间的氮素转移数量、转移途径及影响因素等方面进行了分析与总结,并对可能存在的氮素转移机理进行了综述,对今后的研究方向进行了展望,以期为下一步通过将豆科植物引入我国农牧业种植结构来实现农牧业可持续发展模式的研究提供理论资料。     

Establishment of stylosanthes guyanensis in marandellas sandveld

The feasibility of establishing perennial forage legumes in veld has been investigated at Grasslands Research Station, Marandellas. Of the species tested, Stylosanthes guyanensis (stylo) appeared most suitable.

Reduction of competition from veld grasses was essential for satisfactory establishment of the legume; this was best achieved by shallow discing which also produced a suitable seedbed.

The density of stylo seedlings was affected by seeding rate and degree of soil preparation. More plants established from 4,5 kg/ha of seed broadcast onto disced soil than from 18,0 kg/ha sown onto undisturbed ground. Periodic grazing during the season of establishment had no effect on seedling density. Herbage yields followed the same trends as stylo density. Yields from the plots with the greatest density of stylo plants were more than twice those from the plots with the lowest density.

Seeding stylo into disced veld raised dry matter yields 50% and crude protein yields 300%, although yields of grass were reduced by the addition of the legume. Annual applications of 112 kg superphosphate/ ha had no effect on yields.     

施用豆科绿肥对砖红壤有效磷含量的影响

土壤缺磷是热带和亚热带地区作物生产的主要限制因子之一。本研究分析了14份热带、亚热带豆科绿肥施入土壤后对砖红壤有效磷含量影响的时间动态变化。结果表明,施用绿肥后不同时段能不同程度地提高土壤有效磷的含量,且施用绿肥后的土壤有效磷含量显著高于不施用绿肥土壤,其中木蓝（Magnolia lotungensis）对砖红壤有效磷含量增量变幅高达339%。此外,施用不同的绿肥对土壤有效磷含量的影响不同,但对土壤有效磷含量影响的基本规律一致。施用绿肥时砖红壤有效磷含量提高较大的最佳埋田时间为1个月。     

果洛地区多年生牧草引种试验初报

在果洛地区两个试验点同时进行２５种牧草的引种试验和室内发芽试验。结果表明：１１种禾草和２种豆科牧草表现良好，６种禾草和１种豆科牧草一般，３种禾草和１种豆科牧草农现差。     

Effect of Supplementation of a Basal Diet of Maize Stover with Erythrina variegata,Gliricidia sepium or Leucaena leucocephala on Feed Intake and Digestibility by Goats

Two 4 x 4 Latin square design experiments were carried out. In experiment 1, four mature Anglo-Nubian x Fiji local goats, pre-experimental body weight 25.0 +/- 0.6 kg, 22-24 months old, were used to study the effect of supplementation of a basal diet of maize stover with Erythrina variegata (EV), Gliricidia sepium (GS) and Leucaena leucocephala (LL) on dry matter intake (DMI) and nutrient digestibility. Maize stover treated with urea was used as a control diet. E. variegata was higher in crude protein content than LL or GS. The DMI of the urea treated stover diet was significantly lower (p < 0.05) than that of the diets of untreated stover supplemented with forage legumes. The DMI was significantly lower (p < 0.05) in the GS diet than in the EV or LL diets. Significant (p < 0.05) differences existed between the urea-treated stover and the diets of stover supplemented with forage legumes in the digestibility of dry matter (DM), crude protein (CP), neutral detergent fibre (NDF), organic matter (OM) and energy. In experiment 2, four mature goats, pre-experimental body weight 27.0 +/- 0.3 kg, 24-28 months old, were used to measure their response when the urea-treated maize stover and the maize stover and forage legume diets were sprayed with molasses. The intake of the urea-treated stover diet sprayed with molasses was significantly lower (p < 0.05) that that of the maize stover/forage legume diets sprayed with molasses. The DMI of the diets improved with the addition of molasses. The DMI among the goats offered the maize stover/forage legume diets + molasses did not differ significantly. (p > 0.05). Statistically significant (p < 0.05) differences were obtained in this second study between the urea-treated stover and the stover supplemented with forage legumes in the digestibility of DM, CP, NDF, OM and energy. The stover supplemented with forage legumes had a higher (p < 0.05) nutrient digestibility. The present studies demonstrated that the use of forage legumes as protein supplements improved the feed quality of maize stover in the diets of mature goats. It is suggested that molasses should be sprayed on fresh leaves of Gliricicia sepium and other forage legumes that are initially rejected, in order to improve acceptance and DMI when fed to ruminant animals in confinement or in a cut-and-carry system of production.     

豆-禾混播草地种间关系研究进展

禾本科与豆科牧草的混播,是人工草地建植的最主要方式之一,而了解其之间的种间互作机理,对于维持混播草地的持续稳定生产具有重要意义。本研究从光照、养分、水分和空间等资源方面展开探讨了禾草和豆科牧草在混播时所采取的不同生存策略,阐述了豆—禾混播草地草种之间的竞争与促进关系,分析了豆科牧草固氮和转氮能力的影响因素及深根系豆科牧草与浅根系禾草的共存机制,提出了可以通过混播建植技术、草地管理措施及草地利用方式避免或减弱混播草地豆禾牧草之间的竞争,以此提高混播草地的生产力并维持混播草地持久力和稳定性。     

利用禾草隔离技术防控鼢鼠危害豆科人工草地的研究

豆科牧草来源匮乏始终是制约高寒牧区畜牧业发展的短板之一。除了天然草地资源本身的限制以外,豆科牧草人工草地的建植也存在诸多限制因素,其中一个重要的限制因素就是鼢鼠(Myospalax)危害。研究表明,鼢鼠对豆科等轴根系植物具有强烈的采食偏向性,而对禾本科等须根系植物则表现为相对冷淡。据此,本研究采用在豆科草地外围种植禾本科草隔离带的方式,从草种选择、隔离带宽度、种植方式等技术环节对此种方法的有效性和技术要点进行了探索。结果表明:禾草隔离技术是防控豆科人工草地鼢鼠危害的有效方法,平均可减少危害量82.4%以上;一年生禾草的隔离效果明显优于多年生禾草(P0.01),短期多年生禾草明显优于长期多年生禾草(P0.01);隔离带宽度应控制在3~5m之间;豆科草地建植当年以一年生、短期多年生和长期多年生混播隔离带效果最佳。     

The effect feeding forage legumes as nitrogen supplement on growth performance of sheep

The effect of feeding forage legumes, Cowpea, Silverleaf desmodium and Oxley fine stem stylo, as protein supplements to natural pasture (veld) hay on intake, growth rate and nitrogen metabolism in growing lambs was evaluated. Thirty growing lambs were stratified according to body weight and randomly assigned, within a stratum, to five diets in a completely randomised design. The diets were veld hay alone (V), veld hay supplemented with either 10 g/kg of urea (VU), veld hay supplemented with 250 g/kg Cowpea (VC), 250 g/kg Silverleaf desmodium (VS) or 250 g/kg Oxley fine stem stylo (VF) forage legume hays. The V and the VU groups were used as control diets. Animals supplemented with either urea or the forage legume had higher (P < 0.01) total dry matter intake compared with the animals on V. The animals supplemented with the forage legumes had higher (P < 0.01) nitrogen intake and faecal nitrogen output than the non-supplemented group. All animals, across the treatments, lost body weight; lambs on V had higher (P < 0.01) body weight losses than those in the other treatments. The forage legume supplemented groups lost less (P < 0.01) body weight than those on the V and VU diets. Although supplementation with forage legumes enhanced feed intake and reduced weight losses it did not maintain body weights of lambs fed a basal diet of poor quality roughages.     

青海省海南州天然草地主要饲用植物经济类群及评价

海南州天然草地饲用植物资源丰富,据调查统计有饲用植物３６４种,占全州植物总数的３４．５７％,其中禾本科饲用植物９８种,豆科饲用植物４９种,莎草科饲用植物２７种,杂类草饲用植物１０９种,这四大经济类群是构成海南州天然草地的主体,在各草地类型中占居重要的地位,发挥着各自的优势和作用。     

Evaluation of Illinois Bundleflower (Desmanthus illinoensis) for Broiler Chicks

Illinois bundleflower (IBF; Desmanthus illinoensis) is a warm-season perennial legume native to the central plains of the United States and has potential as both a forage and grain crop. The objective of this research was to evaluate the potential of IBF seeds as a feed ingredient in broiler starter diets. One-day-old broiler chicks were fed 1 of 4 isocaloric and isonitrogenous experimental diets containing 0, 5, 10, or 15% IBF seed. Illinois Bundleflower seed is a high-protein (31.5% CP) feed ingredient with a Lys:Met similar to that of soybean meal. The TME content of IBF seed depends on the form in which it is fed. When fed ground, the TME_n content was determined to be 1,974 kcal/kg. When fed whole, with supplemental grit, the TME_n content was 1,067 kcal/kg. Inclusion of IBF seed in broiler starter diets had adverse affects on both feed consumption and BW gain, even at a 5% inclusion level. Further studies are required to determine whether IBF seed could be included in diets for older broiler chicks or laying hens.     

Effects of legume forages on ovine gastrointestinal parasite development, migration and survival

Lambs grazing certain legumes have reduced parasite intensities compared to lambs grazing ryegrass swards. Eighteen replicates of white clover (cv. AberHerald), lucerne (cv. Luzelle), red clover (cv. Merviot) and perennial ryegrass (cv. Abersilo) were sown at equivalent field rates in 25 cm diameter PVC pots and maintained outside for 6 months. On day 0, forage in each pot was cut to 50 mm from soil level and the pots were placed in a glasshouse (at 19-25 degrees C and 70% humidity) in a randomised block design. Ten grams sheep faeces containing 2,133 Haemonchus contortus eggs per gram were placed on the soil in each pot. Six replicates of each forage were destructively sampled on days 14, 21 and 29. Forage samples were cut at 50 mm from the soil surface and at the soil surface to give two samples per pot. The number of nematodes was determined by a modification of the Whitehead tray method. The ratio of free-living to infective-stage larvae was determined from at least 10% of the larvae. The number of H. contortus larvae kgdrymatter(-1) (DM) forage was calculated and the data rank transformed prior to analysis by ANOVA. There were fewer larvae on legumes compared with ryegrass on samples from forage above 50 mm (P<0.001) but there was no forage effect on larvae below this height. The sum of larvae present on all forage per kilogram DM showed fewer larvae on red clover compared with ryegrass on day 21 (P<0.05). There was an effect of day on the total number of larvae on forage (P<0.001) but there were no foragexday interactions. Analysis of the data according to the leaf area above 50 mm from the soil surface confirmed these results, that there were fewer larvae on legume forages than ryegrass above this height (P<0.01). Overall, red clover affected the development of H. contortus and all legumes affected larval migration above 50 mm compared with ryegrass but survival of larvae was similar on all forages. Further work is needed to determine if these effects of legume forages would reduce the number of parasitic larvae ingested by livestock under field conditions.     

湖南野生豆类牧草资源调查初报

通过对湖南野生豆类牧草资源的种类及分布调查,该地区现有野生豆类牧草92种,隶属31属.其中以决明属Cassin等6种为优势属,以野大豆Glycine soja等6属为优势种,并对6种分布广泛、蕴藏量大且具有开发价值的野生豆类牧草的生物学特征及其饲用价值进行了介绍,以期为综合利用提供重要的参考资料.     

牧草表观遗传学研究进展

表观遗传是指在DNA序列不变的情况下基因表达发生变化的现象。表观遗传现象与外界环境条件的变化紧密相关,它参与植物的生长发育、胁迫响应、衰老死亡等重要生命过程并在其中起到了关键作用。表观遗传学作为一门新兴学科在近20年间得到了快速发展,成为当前动植物和医学领域的研究热点。目前植物表观遗传学的相关研究主要集中在DNA甲基化、组蛋白修饰、RNA甲基化、染色质重塑和非编码RNA修饰等方面,并取得了许多重要成果。然而,相对于模式植物拟南芥和其他主要作物而言,牧草的表观遗传学研究仍处于起步阶段。因此,开展牧草表观遗传学研究对我国草牧业的可持续发展具有重要意义。本研究对表观遗传学的概念、研究方法、研究内容(包括DNA甲基化、组蛋白修饰、RNA甲基化、染色质重塑和非编码RNA修饰等)及牧草表观遗传学相关研究进行了全面总结和综述,并对表观遗传在草牧业中的发展前景进行了展望。