电感耦合等离子-质谱法测定饲料中钠、镁、铬、锰、铁、铜、锌、砷、硒、镉和铅

应用电感耦合等离子-质谱技术(ICP-MS),建立饲料中钠、镁、铬、锰、铁、铜、锌、砷、硒、镉和铅等元素的测定方法。对饲料样品的前处理方法、仪器工作参数和11种元素标准曲线进行优化;并以加标回收、分析方法比对和重复测试说明方法的准确性和精密性。方法在0～1 000 ng/ml范围内线性良好,仪器检出限为0.557 7～5.072 ng/ml,具有良好的精密度,其回收率在88.1%～104.4%之间,相对标准偏差小于5.0%。同时与原子吸收和原子荧光方法进行比对,测定结果相近。所建立的方法简单、快速,可替代原子吸收和原子荧光方法测定饲料中的11种金属元素,为饲料的质量控制提供理想的元素分析方法。     

全国豆粕、玉米、鱼粉、赖氨酸、蛋氨酸价格统计

1月12日,豆粕价格华东2 920～3 000元/吨,华北2 960～3 010元/吨,华南2 940～3 000元/吨,东北3 060～3 360元/吨,中西部2 950～3 150元/吨。玉米价格东北1 830～1 860元     

全国豆粕、玉米、鱼粉、赖氨酸、蛋氨酸价格统计

5月16日，豆粕价格华东3300。3480元／吨，华北3290～3330元，吨，华南3380。3460元／吨，东北3300—3450元／吨，中西部3370～3620元／吨。玉米价格东北2200—2240元／吨，黄淮2200～2460元／吨，南方2530。2660元／吨。国内港口普通级别鱼粉价格9300～9400元，吨，超级蒸气鱼粉10000元／吨。98．5％赖氨酸报价15．00～16．00元／千克，     

全国豆粕、玉米、鱼粉、赖氨酸、蛋氨酸价格统计

7月15日,豆粕价格华东2810~2860元/吨,华北2860元/吨,华南2800~2850元/吨,东北2872~2962元/吨,中西部2850~2940元/吨。玉米价格东北1750~1860元/吨,黄淮1880~2060元/吨,南方1940~2030元/吨。国内港口秘鲁超级蒸气鱼粉11100~11200元/吨。98%赖氨酸报价在7.10~7.40元/千克,70%赖氨酸4.30~4.50元/千克;进口固体蛋氨酸17.80~18.30元/千克。截至7月14日,国内主要港口鱼粉库存总量为24.86万吨。     

全国豆粕、玉米、鱼粉、赖氨酸、蛋氨酸价格统计

3月15日,豆粕价格华东3150-3250元／吨,华北3110～3250元／吨,华南3260～3330元／吨,东北3150～3280元／吨,中西部3200～3320元／吨。玉米价格东北1960～2260元／吨,黄淮2100-2300元／吨,南方2580～2670元／吨。     

全国豆粕、玉米、鱼粉、赖氨酸、蛋氨酸价格统计

9月16日,豆粕价格华东3200～3370元／吨,华北3100～3280元／吨,华南3240～3350元／吨,东北3160～3420元／吨,中西部3270。3420元／吨。玉米东北2140～2290元／吨,黄淮2380～2540元／吨,南方2450～2600元／吨。国内港口普通级别鱼粉价格8000～8200元／吨,超级蒸气鱼粉价格9000～9200元／吨。截至9月11日,     

全国豆粕、玉米、鱼粉、赖氨酸、蛋氨酸价格统计

5月13日,豆粕价格华东2650-2810元/吨,华北2684-2810元/吨,华南2610-2680元/吨,东北2729-2859元/吨,中西部2700-2860元/吨。玉米价格东北1510-1740元/吨,黄淮1750-1940元/吨,南方1 880-1 960元/吨。国内港口超级蒸气鱼粉11 200-11 500元/吨。98%赖氨酸报价在7.20-7.50元/千克,70%赖氨酸5.20-5.60元/千克;固体蛋氨酸19.00-20.00元/千克。截至5月12日,国内主要港口鱼粉库存总量为22.69万吨。     

全国豆粕、玉米、鱼粉、赖氨酸、蛋氨酸价格统计

7月17日,豆粕价格华东3 800～4 070元/吨,华北3 800～3 860元/吨,华南4 000～4 100元/吨,东北3 810～4 030元/吨。玉米价格东北2 280～2 400元/吨,黄淮2 340～2 480元/吨,南方2 630～2 820元/吨。国内港口普通级别鱼粉价格11 000～11 500元/吨,超     

全国豆粕、玉米、鱼粉、赖氨酸、蛋氨酸价格统计

11月18日，豆粕价格华东2920～2980元／吨，华北2930～2960元／吨，华南2920～2970元／吨，东北3040～3220元／吨，中西部3000-3170元／吨。玉米价格东北2220～2280元／吨，黄淮2200～2280元／吨，南方2480～2690元／吨。     

全国豆粕、玉米、鱼粉、赖氨酸、蛋氨酸价格统计

1月15日,豆粕价格华东2 780~2 880元/吨,华北2 800元/吨,华南2 810~2 970元/吨,东北2 900~3 000元/吨,中西部3 000~3 050元/吨。玉米价格东北1 500~1 770元/吨,黄淮1 810~1 920元/吨,南方1 980~2 160元/吨。国内港口超级蒸气鱼粉10 500~10 800元/吨。98%赖氨酸报价在7.60~8.20元/千克;进口固体蛋氨酸18.50~19.00元/千克。截至1月14日,国内主要港口鱼粉库存总量为17.58万吨。     

Comparisons of Angus, Charolais, Galloway, Hereford, Longhorn, Nellore, Piedmontese, Salers, and Shorthorn breeds for weight, weight adjusted for condition score, height, and condition score of cows

Breed differences for weight (CW), height (CH), and condition score (CS) were estimated from records (n = 12,188) of 2- to 6-yr-old cows (n = 744) from Cycle IV of the U.S. Meat Animal Research Center's Germplasm Evaluation (GPE) Program. Cows were produced from mating Angus and Hereford dams to Angus, Hereford, Charolais, Shorthorn, Galloway, Longhorn, Nellore, Piedmontese, and Salers sires. Samples of Angus and Hereford sires were 1) reference sires born from 1962 through 1970 and 2) 1980s sires born in 1980 through 1987. The mixed model included cow age, season of measurement and their interactions, year of birth, pregnancy-lactation code (PL), and breedgroup as fixed effects for CW and CS. Analyses of weight adjusted for condition score included CS as a linear covariate. The model for CH excluded PL. Random effects were additive genetic and permanent environmental effects associated with the cow. Differences among breed groups were significant (P < 0.05) for all traits and were maintained through maturity with few interchanges in ranking. The order of F1 cows for weight was as follows: Charolais (506 to 635 kg for different ages), Shorthorn and Salers, reciprocal Hereford-Angus (HA) with 1980s sires, Nellore, HA with reference sires, Galloway, Piedmontese, and Longhorn (412 to 525 kg for different ages). Order for height was as follows: Nellore (136 to 140 cm), Charolais, Shorthorn, Salers, HA with 1980s sires, Piedmontese, Longhorn, Galloway and HA with reference sires (126 to 128 cm). Hereford and Angus cows with reference sires were generally lighter than those with 1980s sires. In general, breed differences for height followed those for weight except that F1 Nellore cows were tallest, which may in part be due to Bos taurus-Bos indicus heterosis for size.     

Supplementation of broodmares with copper, zinc, iron, manganese, cobalt, iodine, and selenium

In experiment 1, 6 pregnant mares received a concentrate that contained a trace mineral premix that provided 14.3 mg Cu, 40 mg Zn, 28 mg Fe, 28 mg Mn, 0.08 mg Co, 0.16 mg I, and 0.16 mg Se/kg concentrate (group A). Seven mares received the same concentrate plus 502 mg Zn and 127 mg Cu once daily (group B). No differences (P > .05) in foal growth data, or Cu, Zn, and Fe concentrations of mare milk, mare serum, or foal serum were observed. In experiment 2, 6 pregnant mares received the same concentrate as group A (group C), and 8 mares received the same concentrate fortified with 4× the trace mineral premix (group D). Group C mares had higher serum Zn concentration at 1 day (P < 0.01) and 56 days (P < 0.04). Group C mares had higher milk Fe concentration at 28 days (P < .01), and group D mares had higher milk Cu concentration at 56 days (P < .01). Group C foals had higher serum Cu concentration at 14 days (P < .03). The results from this study provide no evidence to indicate that supplementing late gestating and lactating mares with higher dietary trace mineral levels than those recommended currently by NRC has any influence on foal growth and development, or on the Cu, Zn, and Fe concentrations of the mare milk, mare serum, or foal serum.     

Concentrations of strontium,barium, cadmium,copper, zinc,manganese, chromium,antimony, selenium,and lead in the liver and kidneys of dogs according to age,gender, and the occurrence of chronic kidney disease

This study was conducted to measure the concentrations of strontium (Sr), barium (Ba), cadmium (Cd), copper (Cu), zinc (Zn), manganese (Mn), chromium (Cr), antimony (Sb), selenium (Se), and lead (Pb) in canine liver, renal cortex, and renal medulla, and the association of these concentrations with age, gender, and occurrence of chronic kidney disease (CKD). Tissues from 50 dogs were analyzed using inductively coupled plasma mass spectrometry. Cu, Zn, and Mn levels were highest in the liver followed by the renal cortex and renal medulla. The highest Sr, Cd, and Se concentrations were measured in the renal cortex while lower levels were found in the renal medulla and liver. Female dogs had higher tissue concentrations of Sr (liver and renal medulla), Cd (liver), Zn (liver and renal cortex), Cr (liver, renal cortex, and renal medulla), and Pb (liver) than male animals. Except for Mn and Sb, age-dependent variations were observed for all element concentrations in the canine tissues. Hepatic Cd and Cr concentrations were higher in dogs with CKD. In conclusion, the present results provide new knowledge about the storage of specific elements in canine liver and kidneys, and can be considered important reference data for diagnostic methods and further investigations.     

Granulocyte isolation from whole blood of goat, sheep, cattle, horse, dog, pig, and man

A simple two step procedure for the isolation of caprine, ovine, bovine, equine, canine, porcine and human peripheral blood granulocytes is described. After enrichment of granulocytes by centrifugation, contaminating erythrocytes are lysed hypotonically. Recovery, purity, and viability of the granulocyte suspensions are determined. FACScan analysis of the cell suspensions measuring cellular size by forward and sideward light scatter is compared with the corresponding analysis of whole blood leukocytes. Constituencies of the isolated cell suspensions and loss of granulocyte subpopulations through isolation procedure is discussed with regard to granulocyte function assays.     

Department of Clinical Veterinary Practices, School of Veterinary Medicine, University of Dublin, Trinity College, Ballsbridge, Dublin 4

Circular excised skin wounds in the thoracic and metatarsal regions of the dog were studied. A similar sequence of events took place in the two regions although differences did occur due to the different reactions of the tissues which surrounded the wounds. None of the wound cavities became filled with exudate during the early stages of healing. In the thoracic wounds the cavities were largely filled by the swelling and inward movement of adipose tissue. Epithelium first grew on the wound surface in the sector of the wound that was situated in the direction of hair flow. The average time to complete epithelization was similar in both sets of wounds. A zone of alopecia developed around the wounds.