抗鸡传染性法氏囊病病毒酶标抗体的研制与应用

该试验采用过碘酸钠氧化法研制成功了抗鸡传染性法氏囊病病毒酶标抗体。通过进行双抗体夹心法ＥＬＩＳＡ试验，对不同送检病料中的传染性法氏囊病病毒进行了检测。结果表明：用过碘酸钠氧化法制备酶标抗体方法简便、省时；用双抗体夹心法ＥＬＩＳＡ试验检测传染性法氏囊病病毒，具有灵敏度高、特异性强等优点。     

中药和卵黄抗体对传染性法氏囊病病鸡脾脏及法氏囊相对重量的影响

中药和卵黄抗体对传染性法氏囊病病鸡脾脏及法氏囊相对重量的影响祝建新朱印生（江西省中兽医研究所，南昌３３０００６）鸡传染性法氏囊病（ＩＢＤ）是一种主要侵害法氏囊这一免疫器官的疾病。有报道表明，雏鸡感染该病病毒（ＩＢＤＶ）后，法氏囊初期重量增加（肿胀）、...     

卵黄抗体对重庆璧山鸡传染性法氏囊病的防制

鸡传染性法氏囊病卵黄抗体是将传染性法氏囊病毒（IBDV）通过胚胎免疫方法,强化健康鸡免疫、超强化免疫,然后从蛋中提取制成的卵黄抗体。笔者采用卵黄抗体防制传染性法氏囊病取得了较好的疗效,现将2007年至今,笔者用卵黄抗体对重庆市璧山县传染性法氏囊病的防制总结如下。     

蛋鸡法氏囊病的预防和治疗

鸡传染性法氏囊病是由法氏囊病毒引起的雏鸡的一种急性高度接触性传染病。鸡法氏囊病毒主要感染雏鸡．刚出壳的雏鸡有较高的母源抗体保护不容易感染．12周龄以后的鸡法氏囊逐渐退化也不容易感染或成为隐性感染，3～6周龄的鸡最易感，在特殊情况下．1～15周龄的鸡也可被感染。预防该病的主要方法是加强卫生管理和接种免疫工作，治疗的方法主要是肌注抗体和抗生素等。     

免疫器官中肾型鸡传染性支气管炎病毒的定位和动态分布

以鼠抗鸡肾型传染性支气管炎病毒（肾型IBV）的高免血清为一抗，辣根过氧化物酶（HRP）标记的羊抗鼠IgG为二抗，建立了检测石蜡切片肾型IBV抗原的间接酶标抗体染色技术。应用该技术对人工感染肾型IBV C9001株鸡免疫器官中的病毒进行了检测，结果胸腺和法氏囊是病毒主要侵害的免疫器官，脾脏，盲肠扁桃体仅呈一过性感染，哈氏腺未检测到，法氏囊从感染后2－12天，胸腺从感染后3－8天均检测到病毒抗原，阳性染色主要集中于法氏囊淋巴滤泡髓质区和胸腺小叶髓质区淋巴细胞和网状细胞胞浆内。     

立克好中草药口服液治疗鸡传染性法氏囊病试验报告

用立克好中草药口服液治疗鸡传染性法氏囊病试验,从鸡的相对增重率与鸡的临床表现、死亡情况、72h扑杀观察法氏囊损伤程度,10d采血进行抗体效价检测.结果表明立克好中草药口服液能抑制鸡体内法氏囊病毒的生长,缓解鸡传染性法氏囊病的症状.但不能完全杀灭鸡体内传染性法氏囊病毒.试验药物优于对照药物.     

新疆石河子鸡传染性法氏囊病的流行特点及防制对策

鸡传染性法氏囊病是由传染性法氏囊病毒引起的以破坏鸡的中枢免疫器官——法氏囊为主要特征的一种病毒性传染病。该病不仅会造成大量鸡死亡淘汰，影响增重，而且会破坏鸡的法氏囊．从而导致鸡群免疫抑制，使免疫后的鸡群免疫应答下降，抗体水平降低，抗体均匀度差，对大肠杆菌、沙门氏菌、支原体等病原更易感染。     

鸡传染性法氏囊病精制高免蛋黄抗体

抗鸡传染性法氏囊病、鸡新城疫、鸭病毒性肝炎、小鹅瘟等蛋黄抗体在养禽生产上被广泛应用。以前使用的蛋黄抗体为粗制抗体，存在诸多弊端，而精制蛋黄抗体克服了粗制蛋黄抗体的缺点，更加安全、有效、使用方便。2006年洛阳普莱柯生物工程有限公司开发了鸡传染性法氏囊病精制蛋黄抗体．并获得农业部核发的批准文号（2006）160022064．根据国家质量标准在GMP车间生产。     

鸡传染性法氏囊病牛源高免血清直接酶标组化试验

鸡传染性法氏囊病牛源高免血清直接酶标组化试验裴天云，候顺利，赵改敏，刘万恒（兰州军区军事医学研究所，兰州７３００２０）免疫酶组化作为一种快速检测组织中抗原的方法，已成功地应用于马立克氏病病毒、牛鼻气管炎病毒、猪瘟病毒、马传贫病毒和马乙型脑炎病毒等多种...     

应用对流免疫电泳检测鸡传染性法氏囊病病毒抗原和抗体

应用鸡传染性法氏囊病（IBD）血清抗体检测病毒抗原，对流免疫电泳试验（CIET）法比琼脂扩散（AGP）法检出率高50％；而和IBD抗原检测其血清抗体，CIET比AGP有60％血样抗体效价高1－2个滴度，CIET法比AGP法的检测速度快23－37个小时，CIET是一种简易、快速、敏感度较高的检测方法，可用于IBD的检测与诊断。     

中国饲料工业协会第五届理事会顾问、会长、常务副会长、副会长、秘书长、副秘书长、常务理事、理事名单

顾问刘中一张德邻宋瑞祥聂振邦张子仪戎易王维四会长白美清国务院原副秘书长、第四届理事会会长常务副会长(8人,以姓氏笔划为序)刘永好新希望集团董事长、第四届理事会副会长刘汉元通威股份有限公司董事长、第四届理事会副会长陈丹广东恒兴集团股份有限公司董事长、第四届理事会副会长张延喜农业部原副部长、第四届理事会副会长吴明夏湖南正虹科技发展股份有限公司董事长、第四届理事会副会长陶一山唐人神集团股份有限公司董事长、第四届理事会副会长温鹏程广东温氏食品集团有限公司董事长、第四届理事会副会长谭竹洲原化学工业部副部长、第…     

Evidence for the non-involvement of the uterus in the lifespan of the corpus luteum in the cyclic dog

Progesterone levels in peripheral blood of dogs were analysed during the cycle in which hysterectomy (n = 5) or sham surgery (n = 3) was performed as well as during the cycle of dogs (n = 5) hysterectomized at least one year prior to this study; the data were compared with the findings in control dogs (n = 3). The averages of the duration of the luteal period observed in the three experimental groups were not significantly different from those of control dogs. Immediately after surgery, the progesterone level decreased from 25 to 50% of the presurgical level, but returned to presurgical level in about four days. Prolactin levels were elevated for about 30 h after surgery. Nevertheless, the averages of the mean prolactin levels for each animal during the luteal period of the experimental groups were not significantly different from those of control dogs. It is concluded that in the dog, the uterus is not involved in the lifespan of the cyclic corpus luteum.     

The influence of the width of the saddle tree on the forces and the pressure distribution under the saddle

As there is no statistical evidence that saddle fit influences the load exerted on a horse's back, this study was performed to assess the hypothesis that the width of the tree significantly alters the pressure distribution on the back beneath the saddle. Nineteen sound horses were ridden at walk and trot on a treadmill with three saddles differing only in tree width. Kinetic data were recorded by a sensor mat. A minimum of 14 motion cycles were used in each trial. The saddles were classified into four groups depending on fit. For each horse, the saddle with the lowest overall force (LOF) was determined. Saddles were classified as "too-narrow" if they were one size (2 cm) narrower than the LOF saddle, and "too-wide" if they were one size (2 cm) wider than the LOF saddle. Saddles two sizes wider than LOF saddles were classified as "very-wide". In the group of narrow saddles, the pressure in the caudal third (walk 0.63 N/cm(2)+/-0.10; trot 1.08 N/cm(2)+/-0.26) was significantly higher compared to the LOF saddles (walk 0.50 N/cm(2)+/-0.09; trot 0.86 N/cm(2)+/-0.28). In the middle transversal third, the pressure of the wide saddles (walk 0.73 N/cm(2)+/-0.06; trot 1.52 N/cm(2)+/-0.19) and very-wide saddles (walk 0.77 N/cm(2)+/-0.06; trot 1.57 N/cm(2)+/-0.19) was significantly higher compared to LOF saddles (walk 0.65 N/cm(2)+/-0.10/ 0.63 N/cm(2)+/-0.11; trot 1.33 N/cm(2)+/-0.22/1.27 N/cm(2)+/-0.20). This study demonstrates that the load under poorly fitting saddles is distributed over a smaller area than under properly fitting saddles, leading to potentially harmful pressures peaks.     

浅谈对症用药

对症用药就是通过对患病信鸽的症状进行诊断,进而确定病属,选择药物及如何用药的方法。     

军犬鉴别中的嗅源培养和易出现的问题及对策

嗅源是军犬嗅觉作业训练的依据.军犬鉴别中嗅源能力的培养在整个鉴别训练中占有重要地位,关系到鉴别训练的成败和鉴别能力的提高.所以,军犬在嗅认嗅源时必须按照训犬员的指令充分、细致地感受嗅源气味,并且对嗅源气味产生兴奋反应.如何培养军犬充分嗅认嗅源以及及时解决嗅认过程中出现的问题,成为我们进行鉴别训练的关键.……     

Observations on the Pathology of the Spleen of the Cat

Abstract— Lesions were found in the spleen of four of sixty-three cats examined post mortem in the department, and comprised two examples of lymphosarcoma, one of chronic fibrinous peritonitis and one of subcapsular haematoma. Abnormalities were found in two cat spleens received from veterinary surgeons in general practice. In one cat the abnormalities were an haemangiosarcoma with secondary deposits in the liver and in the other extensive mast cell infiltration. In addition lesions were detected in the spleen of three of eighty-seven cats examined post mortem in general practice. The lesions were: multiple secondary deposits of adenocarcinoma from a primary tumour of the mammary gland, a ruptured spleen and a foreign body below the splenic capsule. Résumé— L'examen microscopique de soixante-trois chats pratiqué dans le service a permis de rencontrer quatre fois des lésions de la rate, dont deux lymphosarcomes, une péritonite capsulaire fibrineuse et un hématome sous-capsulaire. D'autre part des lésions ont été trouvées dans deux rates de chat expédiées par des vétérinaires exerçant en pratiaue générale. Chez un chat il s'agissait d'un htmangiosarcome avec métastases hépatiques et chex l'autre d'une infilt ation diffuse par des mastocyta. De plus, on a rencontré des llésions dnas la rate de trois chats parmi quatre-vingt sept autopsies effectuées dans le cadre d'une clientèle génárale. Ces iésions étaient dés métastases multiples d'un adénocarcinome dont la tumeur primitive siègeait dam la glande mammaire, une rupture de la rate et un corps érangers sou capsulaire. Zusammenfassung— Läsionen der Milz wurden bei vier von dreiuiidsechzig in dieser hbteilung sezierten Katzen gefunden, und zwar in zwei Fällen ein Lymphosarkom, in einem Falle eine chron-ische fibrinöse Peritonitis und in einem ein subkapsuläres Hématom. Anomalien fanden sich auch in der Milz von zwei Katzen, die aus der tierxrztlichen Praxis eingesandt wurden. Bei der einen Katze war dies ein Hämangiosarkom mit Metastasen in der Leber, bei der anderen eine weitläufige Infiltration der Mastzellen. Außerdem wurden bei drei von siebenundachtzig in der Praxis sezierten Katzen Milzläsionen gefunden. Diese bestanden am multiplen Metastasen eines Adenokarzinoms der Brustdrüse, einer Milzzerreissung und einem Fremdkörper unterhalb der Milzkapsel.