维生素E和有机硒对小鼠生长性能、应激时抗氧化指标的影响

为了探讨维生素E和有机硒对小鼠生长性能与抗氧化能力的作用,试验选择45只小鼠随机分成3组,分别在基础日粮中添加50 mg/kg维生素E、250 mg/kg有机硒和25 mg/kg维生素E+125 mg/kg有机硒。每周空腹称重,游泳力竭后眼球采血,取内脏称重,测定血液抗氧化指标和脏器指数。结果表明:有机硒组生长性能和饲料利用率均高于维生素E+有机硒组,维生素E+有机硒组高于维生素E组;有机硒组总抗氧化能力(T-AOC)和谷胱甘肽过氧化物酶(GSH-Px)水平显著高于维生素E组(P0.05);丙二醛(MDA)含量各组未达到显著水平(P0.05);除肾脏外,对其他脏器指数无影响。说明有机硒在小鼠生长性能与抗氧化能力方面的作用优于维生素E,有机硒对肾脏的保护作用较强。     

多糖对饲喂高脂饲料小鼠脂肪代谢及氧化应激的改善作用

试验旨在评估高脂肪日粮中添加不同水平的脂肪对小鼠脂肪代谢及氧化应激的改善作用。将初始体重为(22.79±1.75)g的100只小鼠随机分成5组,每组4个重复,每个重复5只小鼠。各组小鼠分别饲喂对照组日粮、高脂肪日粮、高脂肪日粮+20mg/kg多糖、高脂肪日粮+40mg/kg多糖和高脂肪日粮+80mg/kg多糖,试验共开展30d。结果 :高脂肪日粮组小鼠试验结束后的体重较对照组显著提高了15.59%(P 0.05),日粮添加40和80mg/kg多糖较高脂日粮组显著降低血清胆固醇、低密度脂蛋白、甘油三酯和血糖浓度(P 0.05)。此外,对照组、40和80mg/kg多糖较高脂组和20mg/kg多糖组显著提高了血清高密度脂蛋白浓度(P 0.05)。对照组、20、40和80mg/kg多糖组较高脂日粮组显著提高了肝脏、肾脏和肺谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性(P 0.05),但丙二醛含量显著降低(P 0.05)。多糖处理组和对照组较高脂日粮组显著提高了肾脏和肺中维生素C和维生素E含量(P 0.05),但高脂日粮组肝脏维生素C和维生素E组含量显著高于其他组(P 0.05)。结论 :高脂日粮中添加中药多糖在血脂代谢和血糖中起重要作用,同时可以防止小鼠机体自由基反应。     

铜对仔猪血清微量元素水平的影响

为了探讨不同铜源对仔猪生长性能和血清微量元素含量的影响,试验选用体重20 kg左右的军牧1号断奶仔猪75头,随机分成A、B、C、D、E 5组,分别为基础日粮对照组(A组)、基础日粮+125 mg/kg硫酸铜组(B组)、基础日粮+250 mg/kg的硫酸铜组(C组)、基础日粮+125 mg/kg蛋氨酸铜组(D组)、基础日粮+250 mg/kg蛋氨酸铜组(E组),试验期为45 d。在试验第15,30,45天晨饲前空腹采血,分离血清用于测定有关微量元素含量;测定各组猪生长性能。结果表明:日粮添加铜可显著提高血清铜、锌含量(P0.05),对血清铁含量无显著影响(P0.05);125 mg/kg蛋氨酸铜和125 mg/kg硫酸铜的促生长效应较显著(P0.05),且有机铜优于无机铜。说明添加铜对仔猪生长有一定的意义,建议在基础日粮中添加铜125 mg/kg。     

日粮铜源及其水平对猪下丘脑NPY分泌影响的研究

随机选择75头断乳仔猪,分成A、B、C、D和E5组,每组15头猪(设置5个重复).试验基础日粮参照美国NRC (2001)仔猪营养需要配制而成,铜的添加水平为5 mg/kg.A组为基础日粮(对照组),B和C组分别在基础日粮中添加125和250 mg/kg硫酸铜,D和E组分别在基础日粮中添加125和250 mg/kg蛋氨酸铜.添加铜的各组与对照组比较,平均日增质量(ADG)和绝对增质量(AW)均显著高于基础日粮组.猪下丘脑神经肽Y (NPY)含量:A组最低,与各试验组差异显著;B组低于D组(P＜0.01).     

肾损伤病理模型的建立及保肾药保护作用观察

【目的】：通过测定小鼠血液中非蛋白氮（NPN）含量，确立氯化汞对小鼠急性肾损伤模型，并对肌苷和解毒净保肾作用进行比较。[方法】：16只健康昆明小鼠随机分成4组。其中A组为Hgcl损肾组；B组为Hgcl＋肌苷；c组为解毒净＋0．1％HgCl2及D组对照组。24h后处死采血，制备无蛋白血滤液，分别测定血液中非蛋白氮（NPN）含量，从而观察保肾药的作用。【结果]：肌苷和解毒净对氯化汞引起的急性肾损伤都有一定的保护作用。[结论]：解毒净的保肾作用优于肌苷的保肾作用。     

阿魏酸对镉致小鼠急性肾脏损伤的保护作用

为了从氧化应激角度探究阿魏酸对小鼠镉中毒致急性肾脏损伤的保护作用,试验建立氯化镉所致小鼠急性肾脏损伤模型,将80只小鼠随机分为空白组、模型组和阿魏酸低(75 mg/kg)、中(150 mg/kg)、高(300 mg/kg)剂量组,每组16只,空白组、模型组灌胃生理盐水,其余组连续灌胃不同剂量的阿魏酸,除空白组灌胃生理盐水外,其余组均在第15天灌胃氯化镉溶液(按体重0.2 mL/10 g),试验结束后,小鼠摘眼球取血,分离血清,检测血液生理指标[白细胞(WBC)、红细胞(RBC)、血红蛋白(HGB)和血细胞比容(HCT)]、血液生化指标[肌酐(Cr)、血尿素氮(BUN)和尿酸(UA)]。小鼠采血后采取颈椎脱臼法处死,采集肾脏组织,用苏木精-伊红(H.E.)染色后,用医学图像分析系统观察肾脏病理组织学变化;取肾脏组织制成10%和20%的组织匀浆,测定肾脏组织抗氧化指标[还原性谷胱甘肽(GSH)、超氧化物歧化酶(SOD)、丙二醛(MDA)和总抗氧化能力(T-AOC)]。结果表明：与模型组相比,阿魏酸各剂量组血液中WBC、RBC数量及HGB含量、HCT均升高,其中阿魏酸高剂量组WBC数量显著升...     

木犀草素对急性汞中毒小鼠血液生化指标、肝脏及肾脏的影响

试验旨在研究小鼠急性汞中毒后血液、肝脏及肾脏的中毒表现及木犀草素对急性汞中毒小鼠血液指标、肝脏及肾脏的影响。将28只小鼠随机分成4组,分别为对照组(腹腔注射0.9%生理盐水)、木犀草素组(灌胃100 mg/kg木犀草素)、氯化汞组(腹腔注射4 mg/kg氯化汞)和氯化汞+木犀草素组(腹腔注射4 mg/kg氯化汞,灌胃100 mg/kg木犀草素)。检测血清谷丙转氨酶(ALT)和谷草转氨酶(AST)活性,肌酐(CREA)和尿素氮(BUN)水平,血液白细胞(WBC)、红细胞(RBC)和血红蛋白(HGB)水平及肝脏组织谷胱甘肽(GSH)和丙二醛(MDA)水平,并观察肝脏、肾脏组织形态学改变。结果显示,与对照组相比,氯化汞组的血清ALT、AST活性极显著升高(P < 0.01),血清CREA、BUN,血液WBC及肝脏组织MDA含量均显著增加(P < 0.05),血液RBC、HGB及肝脏组织GSH水平均显著降低(P < 0.05),肝脏、肾脏病理变化明显。与氯化汞组相比,氯化汞+木犀草素组的血清ALT、AST活性,CREA和BUN,血液WBC及肝脏组织MDA含量均显著降低(P < 0.05);血液RBC、HGB及肝脏组织GSH含量均显著升高(P < 0.05),肝脏、肾脏病理变化减轻。小鼠急性汞中毒后,其中毒表现为炎症和贫血的发生及肝脏、肾脏不同程度的损伤,木犀草素可减弱急性汞中毒小鼠血液、肝脏和肾脏的毒性作用。     

山豆根多糖对感染PRRSV小鼠脾脏细胞因子水平的影响

探讨山豆根多糖对PRRSV感染小鼠脾淋巴细胞分泌细胞因子水平的影响。将70只昆明种小鼠随机分为7组(A组、B组、C组、D组、E组、F组、G组),每组10只,雌雄各半,依据前期已经建立氧化应激模型的感染条件,D组、E组、F组、G组小鼠于试验第1、2、3天分别采用口服、滴鼻和腹腔注射3种途径联合感染PRRSV病毒原液1.0mL/只,A组、B组、C组给予生理盐水1.0mL/只。第4、5、6天,A、D组小鼠分别腹腔注射生理盐水,0.2mL/10g。B组小鼠腹腔注射5.0mg/kg的脂多糖(LPS)溶液,C组、E组、F组、G组小鼠分别腹腔注射不同剂量的山豆根多糖(200、50、100、200mg/kg)。供试小鼠均于第14天处死,并取其脾脏制备匀浆。采用ELISA检测脾匀浆中TNF-α、IL-1β、IL-6、IL-8、IL-10和MCP-1等细胞因子的水平。结果显示,PRRSV感染小鼠后能升高小鼠脾脏匀浆内TNF-α、IL-1β、IL-6、IL-8、IL-10和MCP-1水平,50mg/kg~100mg/kg剂量的山豆根多糖能降低上述细胞因子的水平。结果表明,山豆根多糖能有效降低PRRSV感染小鼠脾脏细胞因子的水平。     

猫须草叶提取物对高脂饮食诱导小鼠肾损伤的调控作用

为研究猫须草叶提取物(OS)对高脂饮食诱导小鼠肾损伤的调控作用,选择60只雄性小鼠,随机分为对照组(基础饲料)、模型组(高脂饲料)、高剂量组(高脂饲料+500mg/kgOS)、中剂量组(高脂饲料+100 mg/kg OS)和低剂量组(高脂饲料+50 mg/kg OS),每组12只。8周试验期结束后,检测小鼠的肾脏指数,血清中甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)含量,以及小鼠肾组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)含量。通过HE染色观察小鼠肾脏病理学改变。结果表明:与模型组相比,高剂量组小鼠肾小体和肾小管结构更为规则、清晰,肾脏指数显著下降(P0.05),肾脏损伤明显减轻。血清中TG、TC、LDL-C含量显著降低,HDL-C含量显著升高(P0.05)。肾组织匀浆中SOD、GSH-Px活性显著升高,MDA含量显著降低(P0.05)。由此看出,高剂量(500 mg/kg)OS能够较好地降低高脂饮食造成的小鼠肾损伤,这与高剂量OS明显降低了小鼠体内高血脂和氧化应激水平有很大关系,为猫须草副产物动物饲料添加剂的开发提供参考。     

热应激条件下不同抗氧化添加剂对蛋鸡肠道结构的影响

为研究饲粮添加抗氧化剂对热应激条件下蛋鸡肠道结构的影响,选取2 400只35周龄海兰蛋鸡随机分为5组,每组4个重复,每个重复120只,分为A组(200 mg/kg姜黄素+基础日粮)、B组(150 mg/kg添加剂A+基础日粮)、C组(100 mg/kg姜黄素+150 mg/kg添加剂A+基础日粮)、D组(150 mg/kg添加剂A+200 mg/kg添加剂B)和O组(对照组,基础日粮),其中添加剂A含2%茶多酚,添加剂B含≥300亿乳酸菌,7 d预试期,42 d正试期。结果显示:与O组相比,A组蛋鸡空肠、回肠的绒毛高度与黏膜厚度显著提高(P0.05);B组添加剂能够显著提高蛋鸡空肠、回肠、十二指肠的绒毛高度及黏膜厚度(P0.05),且显著提高空肠的绒毛高度/隐窝深度(V/C)值(P0.05);C组蛋鸡空肠、回肠、十二指肠的绒毛高度、V/C值及黏膜厚度显著提高(P0.05)。另外,相对A、B、C组,D组的十二指肠、空肠、回肠的绒毛高度与V/C值均有显著提高(P0.05)。结果表明:姜黄素、茶多酚和微生态制剂等添加剂对热应激条件下蛋鸡肠道损伤有一定缓解作用,且以基础日粮中添加100 mg/kg姜黄素+150 mg/kg添加剂A改善效果最为显著。     

Protective Effect of Saccharum alhagi Against N-acetyl-para-aminophenol induced Liver Injury in Mice

N-acetyl-para-aminophenol (APAP) overdose use induced liver damage.This study was aimed to evaluate the hepatoprotective effect of Saccharum alhagi against APAP-induced liver injury in mice. The aqueous extract of Saccharum alhagi was prepared, ant its ingredients were measured by phenol-sulfuric acid method and aluminium salt chromogenic method. The mice were randomly divided into seven groups,including normal control group,APAP model group,Saccharum alhagi (150, 300 and 600 mg/kg·BW)+APAP groups,positive control group and Saccharum alhagi control group. Administration once per day for 3 consecutive days,with 300 mg/(kg·BW) of APAP after 1 h from the last administration of Saccharum alhagi. 300 mg/(kg·BW) APAP were used to induce liver injury, and after 24 h from APAP challenge,the experimental animals were sacrificed to collect blood and liver tissue samples. The level of serum transaminase (ALT and AST) and the liver pathological changes were observed. The results indicated that the levels of serum AST and ALT were extremely significant lower in the Saccharum alhagi treated groups than the APAP treated group (P<0.01), and the pathological changes were significantly improved. This study showed that Saccharum alhagi could be an effective therapeutic source in APAP-induced liver injuries in mice.     

刺糖对对乙酰氨基酚诱导的肝损伤的保护作用

对乙酰氨基酚（N-acetyl-para-aminophenol,APAP）过量使用能够引起肝脏损伤,本研究旨在探讨刺糖对APAP所致小鼠急性肝损伤的保护作用及潜在的作用机理。采用水提法提取刺糖的有效成分,通过苯酚硫酸法和铝盐显色法进行刺糖提取物成分鉴定;49只雌性小鼠随机分为7组,正常对照组、模型组、刺糖不同浓度的提取物（600、300和150 mg/kg体重）+APAP组、水飞蓟宾阳性对照组及刺糖对照组（600 mg/kg体重）,灌胃3 d,末次给药1 h后,腹腔注射APAP 300 mg/kg体重,24 h后处死小鼠并采集血样和肝脏组织样品。检测血清谷丙转氨酶（ALT）和谷草转氨酶（AST）的活性;用HE染色制作病理标本,观察肝脏病理学变化。结果显示,刺糖提取物能够极显著降低小鼠血清中AST、ALT的含量（P<0.01）,APAP所引起的病理变化明显改善。本研究结果表明,刺糖对APAP所致的小鼠急性肝损伤有一定的保护作用。     

硝酸稀土对湘东黑山羊血液蛋白及非蛋白氮指标的影响

选27只断奶湘东黑山羊随机等分为对照组、试验I组、试验II组,I组按10 mg/kg体重、II组按15 mg/kg体重的剂量添加硝酸稀土添加剂,隔天饮水1次,试验期为30d。定期采血,进行总蛋白(TP)、非蛋白氮(NPN)、白蛋白(A)、血红蛋白(Hb)、球蛋白(G)的测定。结果表明,试验组测定的G值和试验II组的Hb值低于文献报道值,其他测定值在文献报道值内,且TP、A、G值组间差异显著(P<0.05),NPN、Hb值组间差异不显著(P>0.05)。     

Protective Effects of Proanthocyanidins on the Liver and Kidney Oxidative Damage Induced by Zearalenone in Mice

The experiment was conducted to study whether the proanthocyanidin (PC) had the protective effects on the liver and kidney oxidative damage induced by zearalenone in mice.40 8 to 9 week-old healthy clean grade Kunming mice with the 45 g body weight were chosen and randomly divided into four groups: control group was given normal saline,PC group was given 100 mg/kg PC,ZEA group was given 40 mg/kg ZEA and PC＋ZEA group was fed 100 mg/kg PC＋40 mg/kg ZEA.Liver and blood sampls were taken after cervical vertebra to death.AST、ALT、MDA、SOD were measured to determine the degree of liver damage and antioxidant capacity,and UA and BUN were used to determine the kidney damage.The results showed that: Compared with the control group,the indexes of PC group were not significant changed (P>0.05),indicating that there was no obvious oxidative damage in the liver and kidney.Compared with control group,ALT,MDA and AST content were significantly or extremely significant increased in ZEA group (P<0.05;P<0.01),SOD content was extremely significant decreased (P<0.01),UA and BUN were extremely significant increased in serum (P<0.01),indicating that the liver and kidney of ZEA group had a serious oxidative damage.The indexes of the PC＋ZEA group (except SOD)were significantly or extremely significant lower than ZEA group (P<0.05;P<0.01),indicating that the oxidative damage of the liver and kidney in PC＋ZEA group was lower than that in the ZEA group.In conclusion,the liver and kidney oxidative damage of mice induced by ZEA was eased to some extent by PC.     

原花青素对玉米赤霉烯酮致小鼠肝脏、肾脏氧化损伤的保护作用

试验旨在研究原花青素(PC)是否对玉米赤霉烯酮(ZEA)中毒的小鼠肝脏及肾脏具有保护作用.选取40只日龄为8~9周、体重约为45 g的健康清洁级雄性昆明系小鼠,随机分为4组,对照组灌喂生理盐水,PC组灌喂100 mg/kg PC,ZEA组灌喂40 mg/kg ZEA,PC＋ZEA组灌喂100 mg/kg PC+40 mg/kg ZEA,眼球采血,颈椎处死后采取肝脏样本.测定肝脏组织中谷丙转氨酶(ALT)、谷草转氨酶(AST)、丙二醛(MDA)、超氧化物歧化酶(SOD)含量及血清中尿酸(UA)、尿素氮(BUN)含量.结果显示,与对照组相比,PC组各项指标差异均不显著(P>0.05),提示肝脏及肾脏没有明显的氧化损伤;与对照组相比,ZEA组的小鼠组织中谷草转氨酶、谷丙转氨酶及丙二醛含量显著或极显著升高(P<0.05;P<0.01),超氧化物歧化酶含量极显著降低(P<0.01),血清中尿酸、尿素氮含量极显著升高(P<0.01),提示肝脏及肾脏有严重的氧化损伤;PC+ZEA组的小鼠各项指标(除超氧化物歧化酶外)均显著或极显著低于ZEA组(P<0.05;P<0.01),提示其肝脏及肾脏氧化损伤程度低于ZEA组,PC对ZEA中毒小鼠的肝脏及肾脏氧化损伤有一定的保护作用.     

"NPN"补充料对肉牛增重效果试验

[目的]为了验证"NPN"补充料时肉牛增重效果,进行本试验.[方法]试验将西杂牛20头随机分为试验组和对照组,每组10头,试验组在精料中添加"NPN补充料",对照组饲喂常规饲料,不添加"NPN补充料".经过80 d的试验.[结果]表明:试验组肉牛总体重5 116.8 kg,比对照组的4 806.1 kg,多增重310....     

Effect of Luteolin on Blood Indexes,Liver and Kidney in Mice with Acute Mercury Poisoning

To study the effect of luteolin on blood indexes,liver and kidney in mice with acute mercury poisoning,28 mice were randomly divided into four groups:Control group (intraperitoneal injection 0.9% saline),luteolin group (lavage 100 mg/kg luteolin),mercuric chloride group (intraperitoneal injection 4 mg/kg mercury chloride) and mercury chloride+luteolin group (intraperitoneal injection 4 mg/kg mercuric chloride,lavage 100 mg/kg luteolin).The activities of ALT in serum,AST,CREA and BUN contents,blood WBC,RBC,HGB content and GSH and MDA contents of liver tissue were detected.Morphological changes of liver and kidney tissues were observed.The results showed that compared with the control group,the activities of ALT and AST of mercuric chloride group were extremely significantly increased (P < 0.01),serum CREA,BUN,blood WBC and liver tissue MDA contents were significantly increased (P < 0.05) while blood RBC,HGB and liver tissue GSH contents were significantly decreased (P < 0.05).Liver,kidney pathological changes were obviously.Compared with mercuric chloride group,the activities of ALT,AST in serum,CREA,BUN,blood WBC and liver tissue MDA contents of mercuric chloride+luteolin group were significantly decreased (P < 0.05),while blood RBC,HGB and liver tissue GSH contents were significantly elevated (P < 0.05).Liver,kidney pathological changes were attenuated obviously.The poisoning were characterized by inflammation and the occurrence of anemia when acute mercury poisoning occurred,liver and kidney showed different degrees of injury in mice.Luteolin could reduce the toxic effects of acute mercury poisoning on blood,liver and kidney.     

Distribution and amount of cathepsin B in gentamicin-induced acute kidney injury in rats

The aim of our study was to investigate how the distribution and amount of cathepsin B change during acute kidney injury. The research was done on a rat model of acute kidney injury that was induced by nephrotoxic antibiotic gentamicin. Gentamicin was injected at a dose of 40 mg/kg body weight (the first treated group) and 80 mg/kg body weight (the second treated group) for 14 days. Control groups received injections of physiological saline only. One day after the last injection, animals were euthanized, dissected and kidney samples were taken and fixed in 10% buffered formalin. Tissue sections were stained with haematoxylin and eosin, periodic Acid Schiff (PAS) and Oil-red-O. Immunohistochemistry was used for the demonstration of cathepsin B. Vacuolar degeneration of the proximal convoluted tubules was the most prominent pathologic lesion found in the first treated group, while necrosis prevailed in the second treated group in the same localisation. In both treated groups significantly weaker immunohistochemical reaction for cathepsin B was noticed in the proximal convoluted tubules in comparison to the control groups (P < 0.05). The decrease of positive reaction was the largest in the proximal convoluted tubules of the outer renal cortex. Stronger positive reaction for cathepsin B, although not statistically significant, was found in the proximal straight tubules (P > 0.05), as well. However, more numerous cathepsin B-positive large granules appeared in the proximal straight tubules of the second treated group then in the second control group (P < 0.05). We can conclude that the amount of cathepsin B in the affected proximal convoluted tubules significantly decreases along the increased severity of the histopathological lesions of the proximal convoluted tubuls, the amount of enzyme in the well preserved proximal straight tubules increases and more cathepsin B-positive large granules appear in the cytoplasm.     

麦麸阿魏酰低聚糖对敌草快致氧化应激大鼠血浆和组织抗氧化能力的影响

本试验以酿酒酵母和枯草芽孢杆菌作为混合菌种发酵小麦麸皮,通过Amberlite XAD-2柱分离纯化制备麦麸阿魏酰低聚糖(feruloyl oligosaccharides,FOs),探讨麦麸FOs对敌草快(diquat)诱导的大鼠氧化应激是否有缓解作用。试验选用体重相近的断奶雄性大鼠48只,随机分为未攻毒组、攻毒组、攻毒+100 mg/kg BW麦麸FOs组、攻毒+200 mg/kg BW麦麸FOs组、攻毒+300 mg/kg BW麦麸FOs组和攻毒+100 mg/kg BW维生素C组,每组8个重复,每个重复1只鼠,各组大鼠均饲喂相同的商业饲料。麦麸FOs和维生素C配制成水溶液,采用灌胃的方式给予,未攻毒组、攻毒组用生理盐水替代,灌胃体积0.2 mL,连续灌胃15 d。灌胃结束当天,未攻毒组大鼠注射0.3 mL生理盐水,其他5组按0.1 mmol/kg BW的剂量腹腔注射0.3 mL敌草快。敌草快攻毒12 h后取样,分析各组大鼠血浆以及肝脏、肾脏和回肠中总抗氧化能力(T-AOC),过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性以及谷胱甘肽(GSH)和8-羟基脱氧鸟苷(8-OHd G)的含量。结果显示:1)通过混菌发酵小麦麸皮制备麦麸FOs,利用Amberlite XAD-2柱进行分离纯化,获得的麦麸FOs浓度为0.059 mmol/g。2)腹腔注射敌草快显著降低大鼠血浆中SOD活性和GSH含量(P0.05),显著降低大鼠肝脏中T-AOC,CAT、GSH-Px活性及GSH含量(P0.05),显著降低大鼠肾脏中T-AOC及CAT、SOD活性(P0.05),显著降低大鼠回肠中T-AOC,CAT、GSH-Px活性及GSH含量(P0.05),并显著提高大鼠血浆和各组织中8-OHd G含量(P0.05)。3)在敌草快引起的氧化应激状态下,灌胃一定剂量的麦麸FOs可以显著提高大鼠血浆中SOD(400 mg/kg BW)、GSH-Px活性(100和200 mg/kg BW)以及GSH含量(100和200 mg/kg BW)(P0.05),显著提高大鼠肝脏中T-AOC(100、200和400 mg/kg BW),CAT(200和400 mg/kg BW)、SOD(100、200和400 mg/kg BW)和GSH-Px活性(100、200和400 mg/kg BW)以及GSH含量(100、200和400 mg/kg BW)(P0.05),显著提高大鼠肾脏中T-AOC(400 mg/kg BW),CAT(200 mg/kg BW)和GSH-Px活性(200和400 mg/kg BW)以及GSH含量(400 mg/kg BW)(P0.05),显著提高大鼠回肠中T-AOC(200 mg/kg BW),SOD(400 mg/kg BW)和GSH-Px活性(100、200和400 mg/kg BW)以及GSH含量(100、200和400 mg/kg BW)(P0.05),显著降低血浆和各组织中8-OHd G含量(血浆、肾脏、回肠:100、200和400 mg/kg BW;肝脏:100 mg/kg BW)(P0.05);且灌胃200、400 mg/kg BW麦麸FOs后,大鼠血浆和组织中部分抗氧化相关指标可恢复到正常生理状态水平。综上所述,本试验制备的麦麸FOs可以通过有效提高大鼠血浆和组织中抗氧酶活性和GSH含量,降低DNA氧化应激代谢产物8-OHd G的含量,有效缓解由敌草快诱导产生的氧化应激。