日粮中添加硒对断奶南江黄羊羔长性能的影响

选用南江黄羊断奶羊羔10只,按性别、年龄、体重基本一致的原则随机分为对照组和试验组,每组4只公羊,1只母羊,进行45d舍饲饲养试验.试验组在与对照组基础日粮相同的日粮中添加0.15mg/kg的硒.结果表明试验期对照组和试验组只均增重分别为(4.25±0.68)kg、(5.6±0.78)kg,平均日增重分别为93.34g和124.46g,两组间增重差异显著(P＜0.05).     

肉羊暖棚育肥效果试验

选择当年九月龄青年羊60只,平均体重31kg左右,随机抽30只作为试验组,采用塑膜暖棚饲养;另30只作为对照组,采用传统羊床式圈舍饲养。两组均采用(精料0.5kg+苜蓿干草0.8kg+秸粉0.7kg)同一日粮配方,经60d育肥试验,期末试验组平均体重为44.4kg,平均日增重215.2g,对照组平均体重为39.3kg,平均日增重134.2g,增重效果明显。     

双低菜粕浓缩料饲喂生长肥育猪效果研究

85头长大阉割仔猪随机分成3组,第1组为对照组,第2组和第3组为试验组。分别用"8800"、"华双三号"和"中双4号"浓缩料配合成全价饲料,于44～95kg期间进行饲养试验。结果表明:从入试—4月龄左右的试验前期,第1组、第2组和第3组的平均日增重分别为(858.63±153.86)g、(934.41±150.20)g和(895.84±170.33)g;3组的饲料利用率分别为2.74、2.48和2.52。后期各组平均日增重分别为(796±179)g,(685±139)g,(753±186)g;试验全期各组平均日增重(818±105)g、(793±91)g和(811±125)g;饲料利用率3.26、3.21和3.16。利用活体背膘测定仪Piglot105估测的瘦肉率,各组间差异不显著。试验表明利用双低菜粕生产的蛋白质浓缩料可以获得好的生产性能和经济效益。     

育肥羊日粮中添加NSP酶复合预混料对其增重的影响

选择40只育肥羊,随机分为2组,每组20只,试验组日粮中添加占精料量2%的NSP酶复合预混料,对照组不添加。在饲养管理条件完全相同的情况下,经60d试验,结果表明:试验组羊只均增重、平均日增重依次分别比对照组高1.79kg和29.75g,试验组羊增重较对照组提高了30.19%,经检验,差异显著(P<0.05),表明在育肥羊日粮中添加NSP酶复合预混料具有明显的增重效果。     

不同乳猪料对仔猪断奶后1周生产性能的影响

为提高早期断奶仔猪的生长性能,进行饲料对比试验。选择由常熟畜禽良种场提供的28日龄断奶仔猪,6窝(54头),分成试验1和试验2,各27头,断奶体重差异不显著。试验组1用上海征泰实业有限公司生产的“征泰红宝宝”,试验组2用上海某饲料公司生产的“550T”。试验结果表明,试验组1和试验组2,28-35日龄平均日增重分别为(0.21±0.07)kg和(0.18±0.07)kg,差异极显著(P<0.01);28-35日龄平均日采食量分别为(48.38±3.80)g和(44.57±5.61)g;28-35日龄料肉比分别为1.29∶1和2.41∶1,差异极显著(P<0.01)。饲用“征泰红宝宝”比“550T”的效果和经济效益更好。     

日粮中加入荞壳菌糠对生长育肥兔的增重效果的研究

选35日龄的生长育肥哈白兔(体重0.65±0.05kg)150只,按体重、公母随机分成5个组,分别添加荞壳平菇菌糠15%、20%、25%、30%、35%。试验结果表明,在日粮中添加30%的荞壳平菇菌糠,增重效果最好,耗料、增重比最低,与其余各试验组相比差异极显著(P<0.01)。     

日粮中添加蜂花粉饲喂生长育肥猪的试验效果

日粮中添加不同比例蜂花粉的3个试验组生长育肥猪的日增重依次为640.7±65g、690．4±74g、687．1±62g,对照组为601．5±71g,2组和3组与对照组差异极显著（P＜0．01）,1组与对照级差异显著（P＜0.05）,饲料利用率分别比对照组提高1．8％、4.4％、2.4％,经济效益分别比对照组提高5．7％、13．6％和7.2％。     

盐霉素和金霉素对仔猪促生长效果的比较试验

选择72头平均体重(12.95±0.04)kg健康的杜×长×约杂交仔猪,随机分成2个组,每组3个重复,每个重复12头(公母各半)。试验共设2个组,在基础日粮相同条件下,试I组日粮中添加100mg/kg金霉素,试Ⅱ组添加30mg/kg盐霉素。结果表明,30mg/kg盐霉素组与100mg/kg金霉素组相比,平均日增重提高14.37%(P<0.05),饲料增重比和公斤增重饲料成本分别降低l0.04%(P<0.05)和10.29%(P<0.05)。综合各试验组仔猪平均日增重、饲料增重比和经济效益等指标表明,在仔猪饲料中添加30mg/kg盐霉素能获得较好的饲养效果和经济效益。盐霉素由于其药物残留和抗药性少,是较安全可靠的抗生素,应大力推广使用。     

生菌素、维吉尼霉素对哺乳仔猪生长和血清代谢物的影响

将90头平均体重4.98kg哺乳仔猪(杜×大×长)分成2组,日粮中分别添加维吉尼霉素(对照组)和生菌素(普乐宝)。结果表明,与对照组相比,试验组日增重、饲料转化率和血清球蛋白水平提高(P>0.05或P<0.05);仔猪腹泻率、血清尿素氮、血糖和胆固醇含量下降(P<0.05);仔猪每千克增重收益增加2.85%。     

不同日粮能量水平对幼兔生长发育的影响

研究选择90只35日龄的加利福利亚健康幼兔为试验材料,随机分为D1、D2和D33个试验组,每组30只,公母各半。在其它营养水平相同的情况下,3个试验组的日粮能量水平分别设计为消化能11MJ/kg、10.5MJ/kg、10MJ/kg,研究不同能量水平日粮对幼兔日增重、料肉比、腹泻率、死亡率等指标的影响。结果表明,D3组的平均日增重最高(29.84g),D1次之(28.67g),D2最低(26.58g);3个试验组的料肉比分别为4.60:1、4.33:1和3.55:1,而生产1kg兔肉需要饲料成本最低的是D3组。D3组无死亡,腹泻率是3.3%。经济效益分析发现,并依据本研究结果,推荐幼兔饲养的日粮适宜能量需求水平为10.0MJ/kg。     

饲料中添加叔丁基氢醌对大菱鲆生长、血液生化指标、非特异性免疫及肠道组织结构的影响

为研究饲料中添加不同含量叔丁基氢醌(TBHQ)对大菱鲆幼鱼生长、血液生化指标、非特异性免疫及肠道组织结构的影响,在饲料中分别添加0、150、450和750 mg/kg的叔丁基氢醌,配制成4种等氮等脂实验饲料,选择初始体质量(8.31±0.04)g大菱鲆幼鱼,随机分成4组,每组6个重复,每个重复30尾,采用饱饲投喂方式,每天投喂2次,饲养周期为12 W。结果显示,与对照组相比,450和750 mg/kg TBHQ添加组大菱鲆的增重率、特定生长率均显著降低;450 mg/kg TBHQ添加组实验鱼血清碱性磷酸酶活力显著高于150 mg/kg TBHQ添加组;150 mg/kg TBHQ添加组实验鱼血清白蛋白和高密度脂蛋白含量显著低于对照组和450 mg/kg添加组;450 mg/kg TBHQ添加组鱼的血清肌酐含量显著高于对照组;150 mg/kg TBHQ添加组鱼的血清总蛋白含量显著低于对照组;饲料中添加高剂量的TBHQ能够显著升高血清中CAT、溶菌酶活力(450和750 mg/kg)及头肾吞噬细胞呼吸爆发活力(750 mg/kg);饲料中TBHQ添加量为750 mg/kg时,血清SOD活力显著降低;与对照组相比,饲料中添加450和750 mg/kg TBHQ能够显著降低中肠肠道绒毛长度与肠道直径比,而添加750 mg/kg TBHQ时中肠肠道做绒毛长度与肠道直径的比值显著降低。研究表明,饲料中TBHQ的添加量为150 mg/kg时,对大菱鲆幼鱼生长及生理生化指标无显著影响,而饲料中添加450 mg/kg以上的TBHQ则会对大菱鲆幼鱼的生长及生理状况产生一定负面作用。     

凝结芽孢杆菌对奥尼罗非鱼消化酶活性、消化率及生长性能的影响

在基础饲料中分别添加不同剂量的凝结芽孢杆菌(Ⅰ:1.0×1011cfu/kg饲料,Ⅱ:3.0×1011cfu/kg饲料,Ⅲ:6.0×1011cfu/kg),室外水族箱中饲养奥尼罗非鱼(Oreochromis niloticus×O.aureus)(34.50±0.25 g),用基础饲料投喂作为对照,每饲料组设三个重复,每水族箱随机放养16尾鱼,投喂率为3%。采用静水饲养以避免各箱之间水的交换。56 d后测定鱼体的生长和消化酶活性。结果显示:不同添加量的凝结芽孢杆菌均能显著提高奥尼罗非鱼胃、肝胰脏和肠道蛋白酶活性(P<0.05),但酶的活性随添加量的提高呈下降趋势。凝结芽孢杆菌的添加对胃、肝胰脏和肠道淀粉酶及脂肪酶活性没有显著影响(P>0.05)。Ⅰ组和Ⅱ组的干物质表观消化率、蛋白质消化率、相对增重率、饵料系数和蛋白质效率均显著高于对照组(P<0.05),而Ⅲ组和对照组之间差异不显著(P>0.05)。结果提示,饲料中添加1.0×1011cfu/kg饲料的凝结芽孢杆菌就能显著促进奥尼罗非鱼的生长和饲料营养物质的利用,满足最佳生长。     

Effect of supplemental taurine on juvenile channel catfish Ictalurus punctatus growth performance

Juvenile channel catfish (5.6 g/fish) were fed a basal diet that contained major protein (soybean meal, cottonseed meal) and energy (ground corn grain, wheat middlings) ingredients that were derived from plant sources. The basal diet was supplemented with three levels of crystalline taurine to provide 1, 2 and 5 g/kg taurine. In addition, a fifth diet that contained 80 g/kg menhaden fishmeal formulated with the same plant‐source ingredients was included as the positive control diet. Fish were fed the five diets once daily for 12 weeks. Weight gain was highest in catfish fed taurine at 2 g/kg (47.8 g/fish) compared to catfish fed the control basal diet (40.8 g/fish) and 80 g/kg fishmeal diet (41.1 g/fish) (p < .05). Among the taurine‐supplemented diets, weight gain was lowest in catfish fed taurine at 5 g/kg. Feed conversion ratio (FCR) was significantly improved in fish fed diets supplemented with taurine at 2 g/kg compared to all other treatments (p < .01). Survival during the growth study ranged from 98.0 to 99.0% (p > .05) for all treatments. The results suggest taurine supplementation to juvenile channel catfish may improve weight gain and FCR.     

Dietary requirements for leucine,isoleucine and valine (branched‐chain amino acids) by juvenile red drum Sciaenops ocellatus

The objective of this study was to determine the minimum dietary requirements of the branched‐chain amino acids (BCAAs: leucine [Leu], isoleucine [Ile] and valine [Val]) for juvenile red drum, Sciaenops ocellatus. This was accomplished by conducting three independent 49‐day feeding trials with juvenile red drum. Experimental diets were prepared by supplementing a basal diet containing 370 g/kg crude protein from red drum muscle and crystalline amino acids with incremental levels of Leu (9.0, 13.0, 17.0, 21.0, 25.0 and 29.0 g/kg of dry diet), Ile (5.0, 8.0, 11.0, 14.0, 17.0 and 20.0 g/kg of dry diet) and Val (6.8, 8.0, 9.2, 10.4, 11.6, 12.8 and 14.0 g/kg of dry diet). Fish were fed to apparent satiation twice daily in each trial, after which growth performance parameters were calculated and body composition and concentrations of BCAAs in plasma were analysed. Incremental levels of dietary Leu, Ile and Val significantly affected weight gain, feed efficiency and protein retention. Analyses of the weight gain data using a broken‐line regression model estimated the minimum Leu, Ile and Val requirements for maximum growth of juvenile red drum to be 15.7 ± 1.7 g/kg (±95% confidence interval), 11.1 ± 2.3 g/kg and 12.4 ± 0.6 g/kg of dry diet, respectively.     

植物甾醇对团头鲂生长、血脂和肝胰脏抗氧化功能的影响

选取体质量(74.52±1.03)g的团头鲂480尾,随机分成4组,每组4个重复,每个重复30尾,分别投喂添加0(对照组)、20mg/kg(试验Ⅰ组)、40mg/kg(试验Ⅱ组)和80mg/kg(试验Ⅲ组)植物甾醇的配合饲料,进行为期81d的饲养试验。试验结果表明,40、80mg/kg植物甾醇可提高团头鲂质量增加率及特定生长率(P0.05),其中40mg/kg组效果更佳,对肌肉粗蛋白含量影响不显著,对肌肉粗脂肪含量有一定提高(P0.05);添加40mg/kg植物甾醇显著降低了81d血浆低密度脂蛋白胆固醇水平(P0.05)。在本试验条件下,添加40mg/kg植物甾醇效果最佳。     

Changes in the growth performance,antioxidant enzymes and stress resistance caused by dietary administration of synbiotic (fructooligosaccharide and probiotics) in juvenile Chinese Mitten Crab,Eriocheir sinensis

The research aimed to evaluate the effects of dietary fructooligosaccharide, probiotics and their combination on the growth performance, antioxidant enzymes and stress resistance of juvenile Eriocheir sinensis. Crabs (average weight: 9.90?±?0.07 g) were randomly assigned into 4 groups (3 tanks per group) fed experimental diets: basal diet (control group), basal diet supplemented with 5 g/kg FOS, basal diet supplemented with 2 g/kg multi-strain probiotics (each gram contains Lactobacillus acidophilus 10⁶ CFU?+?Bacillus subtilis 10⁷ CFU?+?Saccharomyces cerevisiae 10¹⁰ CFU) and basal diet supplemented with symbiotic (5 g/kg FOS?+?2 g/kg multi-strain probiotics) (designated as control, D1, D2 and D3, respectively). After 8 weeks of feeding trial, crabs were exposed to the transportation process (3 h). Cumulative mortality was recorded at 24 h, 48 h, 72 h and 96 h after transport stress, and pre-transport and post-transport sampling of hepatopancreas occurred for assay. The results indicated that crabs fed D3 had significantly higher weight gain (WG), specific growth rate (SGR) and lower feed conversion ratio (FCR) than crabs fed control diet. Before stress, compared to the control group, crabs fed D1 and D3 diet significantly increased catalase (CAT), superoxide dismutase (SOD) activities and decreased malondialdehyde (MDA) activities, the highest activities of pepsin, trypsin and chymotrypsin were observed in crabs fed D2 diet. After stress, there was a trend that the activities of antioxidants enzymes and proteases in all groups were significantly decreased. And compared to the control group, crabs fed D1 and D3 diet significantly improved SOD and CAT activities, the lowest MDA activity was observed in crabs fed D3 diet, no significant difference was obtained in activities of total antioxidant capacity (T-AOC) and three proteases among all groups (P?>?0.05). At 96 h after transportation process, the lowest cumulative mortality was observed in crabs fed D3 diet, the difference among all groups before and at 24 h, 48 h and 72 h after stress was not significant (P?>?0.05). In summary, a basal diet supplemented with synbiotic could improve the growth performance and protect the hepatopancreas of Eriocheir sinensis more effectively than singular supplementation with prebiotics or probiotics.

     

Juvenile tilapia (Oreochromis niloticus×Oreochromis aureus) requires dietary myo-inositol for maximal growth

An 8-week growth experiment was conducted to estimate the dietary requirement of myo-inositol (MI) for juvenile hybrid tilapia. MI was supplemented at 0, 150, 250, 350, 450, 600, and 1200 mg/kg diet in the basal diet providing 0, 167, 259, 367, 479, 612, and 1253 mg MI/kg diet. Basal diet without MI but with succinylsulfathiazole to suppress MI synthesis by intestinal bacteria was included for comparison. Each diet was fed to triplicate groups of tilapia (mean initial weight 0.51±0.01 g, n=3). Fish fed ≥367 mg MI/kg diet had significantly (P<0.05) higher weight gain, followed by fish fed 259 mg MI/kg diet, and lowest for fish fed the unsupplemented basal diet. Fish fed ≥367 mg MI/kg diet had higher feed efficiency than fish fed the basal diet. Supplementation of dietary inositol did not affect survival of tilapia. The MI concentrations in liver were highest in fish fed the ≥479 mg MI/kg diet, followed by fish fed the 259 and 167 mg MI/kg diets, and lowest in fish fed the basal diet. Hepatic lipid concentrations were higher in fish fed 367 mg MI/kg diet than fish fed ≤259 mg MI/kg diet. Weight gain percentage and MI concentrations in the liver for the different treatments were analyzed by broken-line regression and indicated that the requirement for dietary MI in growing tilapia is about 400 mg/kg diet. Addition of an antibiotic to basal diet did not affect the growth and hepatic inositol concentration of tilapia, suggesting that the intestinal microbial synthesis was not a significant source of inositol for tilapia.     

Dietary combination of pawpaw seed and onion peel powder: Impact on growth,haematology, biochemical and antioxidant status of Clarias gariepinus

A feeding trial was conducted to evaluate the effect of pawpaw–onion powder (POP) mixture on the growth, and haemato‐biochemical and antioxidant responses of Clarias gariepinus (4.02 ± 0.01g/fish) for 60 days. Five trial diets were formulated as control (without POP or antimicrobial growth promoter (AGP)), AGP (basal diet + 10ml AGP/kg diet), POP 2.5 (basal diet + 2.5g POP/kg diet), POP 5.0 (basal diet + 5.0g POP/kg diet) and POP 10 (basal diet + 10g POP/kg diet). Two hundred and twenty‐five fish were equally distributed into five groups in triplicate and fed twice daily. The results indicate that AGP or POP supplementation exerted no effects on the growth and blood profile among the various groups, but a significantly higher lymphocyte count was observed in POP 10g/kg. The highest whole‐body protein and lipid contents were noticed in fish fed the control diet (p < .05), whereas POP 5.0g/kg group recorded the highest hepatosomatic value. Furthermore, the cholesterol level was found to be lower in the AGP‐ and POP‐fed fish compared with the higher level recorded in the control. The glucose concentration and superoxide dismutase enzyme activity were found to be higher in POP 2.5‐fed fish, whereas POP 10‐fed fish showed higher catalase activities compared with other groups (p < .05). Based on the result obtained, this study showed that dietary POP had no significant impact on the growth performance but has direct effects on the whole‐body lipid content, lymphocyte count, cholesterol level, alanine aminotransferase, alkaline phosphatase and antioxidant response of Clarias gariepinus.     

Combined or individual effects of dietary magnesium and selenium nanoparticles on growth performance,immunity, blood biochemistry and antioxidant status of Asian seabass (Lates calcarifer) reared in freshwater

This study was conducted to investigate effects of and interactions between nanoselenium (NanoSe) and nanomagnesium (NanoMg) on growth, humoral immunity, serum biochemistry and antioxidant capacity of juvenile Asian seabass Lates calcarifer reared in freshwater. Four groups of fish with an average weight of 32.78 ± 2.23 g were fed one of the experimental diets for 6 weeks: (a) control (basal diet); (b) NanoSe (basal diet + 4 mg NanoSe/kg diet); (c) NanoMg (basal diet + 500 mg NanoMg/kg diet); and (d) combination (basal diet + 4 mg NanoSe/kg diet + 500 mg NanoMg/kg diet). Fish fed with NanoSe‐supplemented diets (NanoSe and combination) showed higher weight gain, specific growth rate and feed intake. The combination of NanoSe and NanoMg enhanced the immune response. Also, fish fed on combination diet showed higher serum alanine aminotransferase and aspartate transaminase levels but lower glucose concentration. The activities of liver superoxide dismutase, glutathione peroxidase and catalase were not different among all groups, but liver malondialdehyde level was lower in fish fed diets supplemented with NanoSe and/or NanoMg. It could be concluded that NanoSe improved growth performance; the combination of both NanoSe and NanoMg enhanced humoral immunity; and NanoSe and/or NanoMg improved antioxidant capacity in Asian seabass.     

Dietary nucleotide‐rich yeast supplementation improves growth,innate immunity and intestinal morphology of Pacific white shrimp (Litopenaeus vannamei)

An 8‐week feeding trial was conducted to evaluate the effects of dietary nucleotide (NT)‐rich yeast supplementation on growth, innate immunity and intestinal morphology in Pacific white shrimp (Litopenaeus vannamei). Four isonitrogenous and isolipidic practical diets were formulated to contain 0 (control), 10, 30 and 50 g/kg of NT‐rich yeast, respectively. A total of 480 shrimp with an average initial body weight of 1.86 ± 0.02 g were randomly allocated into four groups, with four replicates per group and 30 shrimp each replicate. The results indicated that shrimp fed the diet containing 50 g/kg NT‐rich yeast had significantly higher weight gain (WG), specific growth rate (SGR) and protein efficiency ratio (PER) than those fed the control diet, and the lowest feed conversion ratio (FCR) was observed in the shrimp fed the 50 g/kg NT‐rich yeast supplemental diet. However, there was no significant difference in survival among all treatments. The crude protein of whole shrimp in the 50 g/kg NT‐rich yeast group was higher than that in the control group. Total protein, triglyceride concentrations, the activities of aspartate aminotransferase and alanine aminotransferase in serum were significantly influenced by the dietary NT‐rich yeast supplementation. The activities of serum phenoloxidase (PO) and lysozyme (LZM) of shrimp fed the diet containing 50 g/kg NT‐rich yeast were higher than those in shrimp fed the other diets. Relative expressions of alp and lzm significantly upregulated in the 30 g/kg NT‐rich yeast group compared to the control group. The intestinal fold height and fold width in the 30 g/kg NT‐rich yeast group were significantly higher than those fed the control diet; and the highest microvillus height occurred in the shrimp fed the 50 g/kg NT‐rich yeast diet. In summary, dietary 30–50 g/kg NT‐rich yeast supplementation promotes growth performance, enhances innate immunity and improves intestinal morphology of Litopenaeus vannamei.