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一、微生态制剂的概述
1.微生态制剂的种类我国农业部已批准使用的有益微生物菌种有芽孢杆菌、乳酸杆菌、粪链球菌、酵母菌、噬菌蛭弧菌和脆弱拟杆菌等6大类微生物。目前用作微生态饲料添加剂的微生物主要有:乳酸菌、芽孢杆菌、酵母菌、放线菌、光合细菌等几大类。 相似文献
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青贮是将可用作饲料的青绿植物切碎,在密闭、厌氧条件下,利用乳酸菌发酵作用调制保存的青绿饲料。它不仅保持了青绿植物的优点,并且还具有自身优势,青贮饲料来源广、营养丰富、加工简单,可随时随地进行生产,若能有效应用于渔业,将大大降低渔业的生产成本。青贮饲料在畜牧业中应用广泛,但在渔业生产中的应用还比较少。因此,研究青贮饲料在渔业生 相似文献
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一、微生态制剂的概述1.微生态制剂的种类我国农业部已批准使用的有益微生物菌种有芽孢杆菌、乳酸杆菌、粪链球菌、酵母菌、噬菌蛭弧菌和脆弱拟杆菌等6大类微生物。目前用作微生态饲料添加剂的微生物主要有:乳酸菌、芽孢杆菌、酵母菌、放线菌、光合细菌等几大类。2.微生态制剂的作用机理(1)抑制有害微生物,与有害生物竞争养分和附着部位:微生态制剂在动物消化道内产生有益菌群,与致病菌间就生存和繁殖的空间、时间、定居部位以及营养素等展开竞争,抑制致病菌的生存、繁殖、定居。附着于动物的消化道、呼吸道及皮肤上的有益菌,在代谢过程中产… 相似文献
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<正>青贮玉米制作所占空间小,而且可长期保存,一年四季可均衡供应,是解决牛、羊、鹿等所需青粗饲料的最有效途径。青贮饲料添加剂,因其效果明显,用量较少,作用很大而被广泛应用于青贮饲料调制中,主要可分为发酵促 相似文献
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乳酸菌是鱼消化道固有菌群的组成部分,能定植于鱼类消化道,拮抗革兰氏阴性致病菌,维持肠道正常的微生态平衡.鱼消化道中比较常见的乳酸菌种类有肉杆菌、乳酸杆菌等.综述了乳酸菌在不同生长阶段鱼消化道的分布、鱼类消化道乳酸菌分布的影响因素和鱼类消化道乳酸菌的功能. 相似文献
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乳酸菌对牙鲆稚鱼养殖水体和肠道菌群的影响 总被引:7,自引:1,他引:7
在牙鲆稚鱼中投喂添加由单一鼠李糖乳杆菌P15制备的微生态制剂和黄霉素,在60d的投喂期内,采用平板计数法检测好氧性异养菌的总数、总弧菌数和乳酸菌的数量。结果表明,在投喂菌液和冻干菌粉后,养殖水体和牙鲆肠道的乳酸菌数均呈上升趋势,在30d后乳酸菌数量达到稳定并在肠道内定植。同时,由于乳酸菌的抑制作用,弧菌的数量下降,以肠道中的弧菌最明显。乳酸菌对养殖水体和牙鲆肠道的好氧性异养菌没任何影响。乳酸菌对养殖水体和肠道菌群的影响与抗生素具有相近的效果,表明乳酸菌作为饲料添加剂可以取代抗生素应用在牙鲆的养殖中。 相似文献
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Sergio E Pasteris Germán Roig Babot María C Otero Marta I Bühler & María E Nader-Macías 《Aquaculture Research》2009,40(14):1605-1615
This work addresses the selection of potentially probiotic lactic acid bacteria (LAB) to be used in raniculture. Thus, strains belonging to the genera Pediococcus pentosaceus, Leuconostoc mesenteroides, Lactococcus lactis and Enterococcus faecium isolated from a Rana catesbeiana hatchery were evaluated for their inhibitory properties against RLS-associated pathogens ( Proteus vulgaris, Pseudomonas aeruginosa, Staphylococcus epidermidis ) and food-borne bacteria. Cell-free supernatants of LAB strains inhibited the growth of at least one of the pathogens by organic acids, but L. lactis CRL 1584 also produced a bacteriocin-like metabolite. The ability of LAB strains to produce H2 O2 in MRS+TMB medium was also studied. Seventy-eight to ninety six per cent of the strains showed some level of H2 O2 production. Moreover, different organic solvents were used to determine the hydrophobicity and Lewis acid/base characteristic of LAB strain surfaces. Most of the strains presented hydrophilic properties, but no acidic or basic surface characters. However, some strains isolated from the skin showed a high degree of hydrophobicity and basic components in the cell surface due to their adhesion to chloroform. These properties were not observed in LAB from balanced feed and freshwater. Taking into account general guidelines and the beneficial properties studied, five strains were selected as potential candidates to be included in a probiotic for raniculture. 相似文献
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ABSTRACT: Lactic acid fermentation of seaweed is a recent topic and quite limited information is available on culture conditions. To know the suitable strains for use as a starter culture for seaweed fermentation, 14 lactic acid bacteria (LAB) strains, including 11 species, were tested in culture conditions prepared with or without salt. A commercial product of Undaria pinnatifida powder was used as a substrate for fermentation without sterilizing. Starter-suitability of the LAB strains was assessed from their predominance after culture. Among the tested strains, Lactobacillus brevis, Lactobacillus plantarum, Lactobacillus casei and Lactobacillus rhamnosus showed high (>90%) predominance in their cultures, while control cultures prepared without inoculation of LAB did not show any detectable growth of acid producing bacteria and spoiled. A total of 102 strains not showing acid producing activity were isolated and characterized from spoiled cultures, and all the isolates were observed as Bacillus strains, including 64 strains (62.7%) and 16 strains (15.7%) of a Bacillus cereu s-related and B. fusiformis -related species, respectively. The Undaria substrate before fermentation contained culturable microorganisms at 1.4–3.1 × 102 CFU/g, but the Bacillus cereu s-related strain was not a major composition, suggesting a concern of selective growth of the Bacillus cereu s-related strain during the spoiled fermentation. 相似文献
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Identification and partial characterization of potential probiotic lactic acid bacteria in freshwater Labeo rohita and Cirrhinus mrigala 下载免费PDF全文
Muhammad Shahid Bilal Hussain Danish Riaz Mohsin Khurshid Muhammad Ismail Mohsin Tariq 《Aquaculture Research》2017,48(4):1688-1698
Carps are the most diversified freshwater fish belonging to family Cyprinidae. Numerous probiotic and pathogenic lactic acid bacteria (LAB) have been characterized from carps. However, the diversity of these ecologically important bacteria is entirely unknown in freshwater fish of Pakistan. The present study aimed to characterize and identify the lactic acid bacteria from two carps viz. Laboe rohita and Cirrhinus mrigala and determine their antagonistic activity. Seventeen bacterial isolates were purified from the gastrointestinal tract and gills of these fish and characterized morphologically. Initially, seven isolates were screened as LAB using agar supplemented with CaCO3. Subsequently, only two isolates CILB2 and RIL10 were selected as LAB after high‐performance liquid chromatography analysis for lactic acid production. Isolates CILB2 and RIL10 were genetically identified as Enterococcus faecalis and Weissella sp., respectively after 16S rRNA gene sequence analysis. Both strains exhibited significant antagonistic activity against common fish pathogens Streptococcus agalactiae, Staphylococcus aureus and Escherichia coli. Enterococcus faecalis CILB2 and Weissella sp. RIL10 were also found negative for haemolysis and gelatinase activities and were sensitive to ampicillin, amoxicillin, doxycycline, erythromycin, chloramphenicol and co‐trimoxazole antibiotics. The identified LAB strains may further be investigated for their potential probiotic application in fish feed and food preservation techniques. 相似文献
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Su Myo Thwe Takeshi Kobayashi Tianyao Luan Takaaki Shirai Munenaka Onodera Naoko Hamada-Sato Chiaki Imada 《Fisheries Science》2011,77(2):279-288
γ-Aminobutyric acid (GABA)-producing lactic acid bacteria (LAB) were isolated from four types of Myanmar traditional fermented
fishery products with boiled rice. All of them belonged to the genus Lactobacillus, and comparison of the effects of these representatives on GABA accumulation in fermented fishery products with boiled rice
revealed that Lactobacillus farciminis D323 is the most effective strain as a starter culture. These results may contribute to the development of traditional fermented
fishery products with functional properties. In addition, this study is the first to show in detail the distribution of GABA-producing
LAB in Southeast Asian fermented fishery products. 相似文献
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M. Planas J.A. Vzquez J. Marqus R. Prez-Lomba M.P. Gonzlez M. Murado 《Aquaculture (Amsterdam, Netherlands)》2004,240(1-4):313-329
One of the pathways for the entry of bacteria, both pathogenic and probiotic, into the larvae of fish hatcheries is via live prey. As a preventive measure against infections, live prey may be cultured, supplementing the food with probiotics. Some lactic acid bacteria (LAB) have been successfully used in the larviculture. In this study, the nutritional effect of seven terrestrial LAB has been studied regarding the growth of the rotifer Brachionus plicatilis. The cultures were carried out without partial renewal of the culture medium, feeding the rotifers on baker's yeast and adding some of the species of bacteria. In all cases, the addition of the bacteria increased both the specific maximum growth rate and the maximum density obtainable in the cultures. However, the best results were obtained with the addition of Lactococcus casei ssp. casei, Pediococcus acidilactici, or Lactobacillus lactis spp lactis. The rates of growth obtained with the individual or joint addition of these three bacteria were 8–13 times greater than those obtained with the control cultures after 4–5 days' culture. In this study, a series of kinetic models has been applied (logistic modified—Gompertz, logistic–logistic, and generalised logistic) which describes the experimental data, obtaining a set of parameters of biological significance which facilitate the optimisation of the use of these bacterial strains in the mass production of rotifers. 相似文献
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Juan Pablo Apún-Molina Apolinar Santamaría- Miranda Antonio Luna-González Sergio F. Martínez-Díaz & Maurilia Rojas-Contreras 《Aquaculture Research》2009,40(8):887-894
This study examined the effect of potential probiotic bacteria on growth and survival of the tilapia Oreochromis niloticus , under high density and suboptimum temperature. Presumptive Bacillus and lactic acid bacteria (LAB) were isolated from tilapia and from their culture system and were characterized for haemolytic and enzymatic activity, and antagonism against Vibrio . Selected strains were included in the diet of juvenile tilapia and evaluated during a 134-day assay. The experiment was conducted with four treatments: (1) fish fed with commercial feed plus Dry Oil® ; (2) fish fed with commercial feed plus LAB; (3) fish with bacilli in water; (4) fish with a mixture of treatments 2 and 3. Tilapias in all treatments, including bacteria, grew significantly better than fish fed with commercial feed plus Dry Oil® (control group). Survival was similar in all treatments. The physicochemical parameters of the culture system were maintained within the optimal ranges for the species, with the exception of temperature (19.9–24.82 °C). Animals fed diet supplemented with bacilli and LAB had good survival and the best growth performance, suggesting that bacteria are appropriate growth-stimulating additives in tilapia cultivation. 相似文献
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Gülsün Özyurt Esmeray Kuley Boga A. Serhat Özkütük Mustafa Durmuş Yılmaz Uçar 《Journal Of Aquatic Food Product Technology》2019,28(3):314-328
The impacts of fermentation process with acid and lactic acid bacteria strains (Lactobacillus plantarum, Lactobacillus brevis, Pediococcus acidilactici, Enterococcus gallinarum, and Streptococcus spp.) on the biogenic amine formation of wet and spray-dried fish silage obtained from whole gibel carp (Carassius gibelio, freshwater discard), whole ponyfish (Equulites klunzingeri, seawater discard), and processing by-products of seabass (Dicentrarchus labrax) were investigated. The results showed that among biogenic amines, cadaverine, putrescine, spermidine, spermine, serotonin, dopamine, and agmatine were predominant in all groups. Significant differences (p < 0.05) in biogenic amine concentrations of wet and spray-dried fish silage were observed. Raw fish and wet silages contained histamine level lower than the allowable limit of 50mg/kg, indicating the use of raw fish material with low microbial counts. In addition, no histamine was detected in spray-dried fish silage, except for seabass by-products with a trace quantity of histamine (<0.04mg/100g). The starter culture used for silage preparation did not effectively retard formation of biogenic amines compared to acid silage. It can be concluded that there is potential use of fermented fish silage as a protein source and possibly as a probiotic ingredient for animal feed in both wet and dry form. 相似文献
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Effects of dietary mono‐ and multiprobiotic strains on growth performance,gut bacteria and body composition of Javanese carp (Puntius gonionotus,Bleeker 1850) 下载免费PDF全文
S.K. Allameh F.M. Yusoff E. Ring H.M. Daud C.R. Saad A. Ideris 《Aquaculture Nutrition》2016,22(2):367-373
This study investigated the effects of dietary mono‐ and multiprobiotic strains of Enterococcus faecalis, Lactobacillus fermentum and Leuconostoc mesenteroides on growth performance, intestinal bacteria and body composition of Javanese carp (Puntius gonionotus). Moreover, the effect of storage temperature on lactic acid bacteria (LAB) viability in the feed was studied. Compared to the control group, significant (P < 0.05) effect on weight gain, feed conversion ratio, specific growth rate and protein efficiency ratio of probiotic fed Javanese carp was observed. Inclusion of individual LAB strains in the diet resulted in significantly improved growth performances of Javanese carps compared to fish fed a diet supplemented LAB mixture. The inclusion level of E. faecalis at 107 colony‐forming unit (cfu) g−1 in the diet revealed higher growth performance compared to other treatments. The population levels of LAB in the fish intestine were significantly increased while the levels of Gram‐negative bacteria were significantly decreased compared to the control. No significant variations were observed in the chemical composition of the carcass by probiotics. The viability of LAB in feed was higher when stored at 4 °C compared to 25 °C. It is suggested that E. faecalis has a greater potential for use in improving Javanese carp culture outcomes. 相似文献
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Amit Kumar Rai R. Jini H. C. Swapna N. M. Sachindra V. Baskaran 《Journal Of Aquatic Food Product Technology》2013,22(1):32-44
Fermentation using native lactic acid bacteria (LAB) was evaluated for its effectiveness in recovering lipids and proteins simultaneously from freshwater fish visceral waste (FVW). Five different LAB isolated from fish processing waste were employed in a fermentation process that involved 10% (w/w) glucose, 2% (w/w) NaCl, and 10% (v/w) LAB. Cultures evaluated included four native isolates (Pediococcus acidilactici NCIM5368, Enterococcus faecalis NCIM5367, Pediococcus acidilactici FM37, and Pediococcus acidilactici MW2) from FVW with E. faecium NCIM5335 as the reference culture. Fermentation with native LAB resulted in recovery of > 90% oil present in the material as against no recovery in case of raw (unfermented) viscera and resulted in > 50% of degree of hydrolysis of proteins. The fatty acid profile of lipids was not affected by the fermentation process. The fermentation liquor, rich in hydrolyzed protein, exhibited antioxidant as well as antagonistic properties against several bacterial pathogens. The results clearly demonstrate the usefulness of fermentation using native isolates for simultaneous recovery of lipids and proteins from fish processing waste. It also asserts the value of fermentation as an eco-friendly method and aids in minimizing disposal/pollution problems associated with these solid wastes. 相似文献