东海区黄鲫数量分布特征的分析研究

本文以东海区2000～2002连续3年4个季节的底拖网渔业资源调查资料为依据，分析研究了黄鲫资源数量的时空分布特征及其与水深和水温的关系，在此基础上进一步推测了东海区北部海域与中南部海域黄鲫的洄游规律差异。结果表明，东海区黄鲫的数量分布不仅有明显的季节差异，而且以长江口渔场的南端为界．东海区北部海域和中南部海域的黄鲫群体之间存在显著的生态差异。夏季，两个群体在吕四、长江口和舟山渔场20～95m水深的近海海域集中产卵，产卵适温为19．5～25．5℃，并有混栖现象。秋季，东海北部群体沿东西向作近外海索饵洄游(水深：25～100m，适温：22．5～23．5℃)；而中南部群体沿西北向东南海域进行索饵洄游(水深：60～150m，适温：26～27.5℃)。冬季，北部群体越冬现象不明显，适温为10～12℃；中南部群体存在明显的越冬行为，越冬场主要分布在鱼山、鱼外和舟外渔场。至春季两个群体又均表现出向产卵场聚集的洄游趋势。     

东海渔场环境与太平洋丛柔鱼的分布

太平洋丛柔鱼(Todarodes pacificus steenstrup)的年产量,在八十年代高达四、五十万吨,占目前世界头足类年产量的三分之一左右,是世界最重要的头足类资源。丛柔鱼的分布区局限于太平洋,主要分布在日本群岛周围海域,其群体密度最高;在黄海北部和东海外海的分布区,也有一定产量,主要为流网和底拖网所兼捕。本文论述东海长江口渔场、舟山渔场内太平洋丛柔渔资源的分布密度以及它与不同水系交汇形成流隔的密切关系;五、六月份柔鱼鱼发适温为16—17℃,柔鱼群体栖息活动水层与浮游     

东海区七星底灯鱼数量分布以及与温盐度的关系

本文利用从2000年12月到2001年9月一个周年的渔业资源调查数据,对东海区七星底灯鱼的数量分布及其与温盐度的关系进行了研究。结果表明:东海区的七星底灯鱼可分为东海北部和浙江中南部近海2个群体。以东海北部群体较大,主要分布在30°N以北的海域;浙江中南部近海群体较小,且只有零星分布。七星底灯鱼在东海北部海域从沿岸到外海都有分布,但沿岸数量较少,主要分布在30°30′~32°30′N、124°00′~126°30′E之间的海域;以冬春季节分布面较广,夏秋季节的分布面较窄。东海北部群七星底灯鱼栖息海域的表层温度值变化较大,以春季最低,秋季最高;密集分布区的等温线变化范围在1~2℃左右。浙江中南部近海群的栖息水温变化较小,全年都在20℃以上。各季节浙江中南部近海群的适宜水温均高于东海北部群。各季节东海北部群密集分布区的表层盐度在32.0~34.5之间,以冬春季的盐度值较高,夏秋季的盐度值较低;浙江中南部近海群的栖息海域表层盐度除冬季较高外,其余季节相对较低。     

太平洋褶柔鱼渔业资源及渔场学研究进展

太平洋褶柔鱼(Todarodes pacificus)是典型的温带大洋性头足类,主要由秋生群体和冬生群体组成。目前该物种的主要栖息地分布于日本海及太平洋沿岸海域,而我国黄海和东海外海为该物种的主要产卵场,调查发现此海域的太平洋褶柔鱼资源量呈逐年上升趋势,已经成为潜在的重要种类之一。为了后续更好地开发和利用该物种,整理了前人对太平洋褶柔鱼多方面的研究,从基础生物学、渔业及其与海洋环境的关系和资源评估管理等几个方面进行了回顾和总结。结果认为,目前太平洋褶柔鱼主要捕获海域仍然集中在日本和韩国沿岸,产量在80年代后期和90年代前期经历了较大的波动后,近十多年保持在40×104t左右。不同群体有着不同的洄游路径,其受环境影响的程度也有所不同。目前对日本沿岸太平洋褶柔鱼的年龄、生长、繁殖等特性有着较为全面的研究,但仍然需要对其进行长期监控。大尺度海洋环境变化会对太平洋褶柔鱼的CPUE有较大的影响,而通过对其资源状况的评估,可以更好地管理该资源,实现可持续利用。本文可对我国今后在东黄海海域合理开发该资源提供相关依据,也可为今后我国在与邻国的渔业谈判中提升更多的话语权。     

浙江南部外海头足类种类组成和数量分布

根据2006年5月、8月、11月和2007年2月浙江南部外海底拖网渔业资源调查资料,用渔获率作为资源分布的数量指标,对浙江南部外海头足类种类组成、数量分布、季节变化趋势作了定量分析。结果表明:浙江南部外海调查海域头足类种类有36种,其中:群体数量较大,经济价值较高的常见种有杜氏枪乌贼、剑尖枪乌贼、中国枪乌贼、长蛸、针乌贼、真蛸、神户乌贼、珠乌贼、罗氏乌贼、短蛸、椭乌贼、太平洋褶柔鱼、多钩钩腕乌贼等13种。调查海域以夏季头足类资源密度较高,数量分布主要集中在100~200 m水深的外侧海域;秋季和冬季尽管头足类资源数量在下降,但其数量的地理分布与夏季相近;而春季分布较密集的区域则在80~200 m水深海域。年间不同水深海域的头足类资源密度是80~100 m水深海域低于100~200 m水深海域。与近海渔场相比,浙江南部外海调查海域头足类种类多而且资源较丰富,其渔获量仅次于鱼类,是浙江南部外海底拖网的主要捕捞对象,特别是枪乌贼科的种类是今后可供底拖网作业开发利用的主要渔业资源之一。     

东海发光鲷生物量分布及其与环境的关系

根据2005年12月~2006年11月东海(27°00′~34°00′N、122°00′~127°00′E)底拖网资源调查资料,分析了东海发光鲷的生物量的分布特征以及与环境条件的关系。结果表明:春、秋季发光鲷主要分布在东海南部外海,生物量较高;夏季分布较广,以东海北部外海为主,生物量较低;冬季分布较为集中,以东海南部外海为主。夏季发光鲷的生物量与温度、盐度以及水深的关系的曲线拟合显示,夏、秋季发光鲷主要分布于24℃左右的水温,最适盐度在33左右,最适水深在75 m左右。     

东海北部头足类的种类组成和数量分布

本文根据１９９４年６月至１９９６年５月东海北部２５°３０′～３３°３０′Ｎ，１２８°００′Ｅ以西海域的调查资料，阐明了头足类的种类组成，主要种类数量的季节变化和时空分布，结果显示，东海北部头足类资源群体结构已发生变化，原先的优势种类日本无针乌贼资源已衰退，而剑尖枪乌贼，太平洋褶柔鱼，金乌贼等已成为新的捕捞对象，文章还介绍了主要种类的渔场渔期可持续发展的管理对策。     

东海区头足类群聚空间分布特征的初步研究

根据2000年东海区(27°00′~34°00′N、127°00′E以西海域)150 m等深线以浅4个季节的底拖网监测调查资料,运用聚类分析和非度量多维标度(NMDS)的方法分析东海区头足类群聚的空间分布特征,并且探讨各个群聚类型的指示种类以及头足类群聚分布与环境的关系。头足类主要种类为剑尖枪乌贼(Loligo edulis)、太平洋褶柔鱼(Todarodes paci-ficus)、金乌贼(Sepia esculenta)等。多元分析结果显示,各季节头足类群聚类型不同。根据各群聚类型分布的海域大致可以将其划分为黄海南部-东海外海-东海中南部群聚、长江口外海域群聚和东海北部海域群聚3种群聚类型。它们的空间分布区域随着季节的不同而发生变化,群聚类型的分布区域也会相互交错。各季节不同群聚类型的种类组成不同,多数种类一般同时出现于不同的类群中,有的种类在所有群聚类型中都有分布。它们的空间分布也是交错的,只是相对集中的区域有所不同,在不同群聚类型中的渔获率和出现频率不同。头足类各群聚类型种类组成的季节差异较明显,各群聚类型中相对稳定的主要特征种类较少。调查海域中影响头足类种类空间分布特征的主要环境因子为水温,而水深等环境因素的影响并不明显     

东海北部鱼类组成特征分析

利用1997-2000年东海北部(29°30′～33°00′N,122°30′～128°00′E)四个季节的底拖网调查资料,选取生物量占总生物量90％的前27种鱼类,运用聚类分析和多维标度分析法,研究了东海北部鱼类组成的空间格局特征。结果显示东海北部鱼类组成可分为三个群聚类型,即近海群聚、较深海域群聚和外海群聚。近海群聚的主要种类有凤鲚(Coilia mystus)、龙头鱼(Harpadon nehereus)、黄鲫(Setipinna taty)等;较深海域群聚的主要种类有鳀(Engraulis japonicus)、带鱼(Trichiurus japonicus)、日本鲭(Scomber japonicus)、鳄齿鱼(Champsodon capensis)、细条天竺鲷(Apogon lineatus)、发光鲷(Acropoma japonicum)等;而竹(竹夹)鱼(Trachurus japonicus)、星康吉鳗(Conger myriaster)、黄鲷(Taius tumifrons)、水珍鱼(Argentina kgoshimae)、日本海鲂(Zeus fober)、绿鳍马面鲀(Thamnaconus sepremtrionalis)等为外海群聚的主要种类。同时分析了水深、底层温度、底层盐度等环境因子与鱼类群聚的关系,表明影响鱼类群聚的主要环境因子有水深、底层盐度。不同群聚间的种类分布是重叠交错的,并没有明显的群聚边界,但密集分布海域各异,不同群聚的种类组成在不同季节具有一定的持续性,其种类组成保持了相对的稳定。     

东海北部和黄海南部Ti鱼卵和仔稚幼鱼数量、分布及其与环境条件的关系

1999年夏季鳀鱼卵和仔稚幼鱼主要分布于黄海南部,122°00′～123°45′E、34°00′～35°15′N与122°15′E以西海域分别为鳀鱼卵与鳀鱼仔稚幼鱼的密集分布中心,其表层水温和盐度分别为25.5～26.9℃和27.11～31.81与26.4～27.0℃和31.31～32.05。鳀鱼卵和仔稚幼鱼分布的最适表层水温分别为25.5～26.4℃和25.9～27.0℃;全长为20mm以上的鳀鱼稚鱼已初步形成广盐性分布的习性,鳀鱼卵和仔稚幼鱼的分布对盐度的要求并不非常严格,水温是影响鱼卵和仔稚幼鱼分布的主要原因之一。鳀鱼卵和仔稚幼鱼的分布与叶绿素a的分布也有密切的关系,鳀鱼卵和仔稚幼鱼的密集分布中心正是叶绿素a的高值区。随着季节的变化,黄海南部鳀鱼产卵场由青岛外海逐渐向深水区转移。黄海南部鳀鱼活卵所占的比例为45.76％,胚胎发育状况良好。     

Association between the interannual variation in the oceanic environment and catch rates of bigeye tuna (Thunnus obesus) in the Atlantic Ocean

The environmental processes associated with variability in the catch rates of bigeye tuna in the Atlantic Ocean are largely unexplored. This study used generalized additive models (GAMs) fitted to Taiwanese longline fishery data from 1990 to 2009 and investigated the association between environmental variables and catch rates to identify the processes influencing bigeye tuna distribution in the Atlantic Ocean. The present findings reveal that the year (temporal factor), latitude and longitude (spatial factors), and major regular longline target species of albacore catches are significant for the standardization of bigeye tuna catch rates in the Atlantic Ocean. The standardized catch rates and distribution of bigeye tuna were found to be related to environmental and climatic variation. The model selection processes showed that the selected GAMs explained 70% of the cumulative deviance in the entire Atlantic Ocean. Regarding environmental factors, the depth of the 20 degree isotherm (D20) substantially contributed to the explained deviance; other important factors were sea surface temperature (SST) and sea surface height deviation (SSHD). The potential fishing grounds were observed with SSTs of 22–28°C, a D20 shallower than 150 m and negative SSHDs in the Atlantic Ocean. The higher predicted catch rates were increased in the positive northern tropical Atlantic and negative North Atlantic Oscillation events with a higher SST and shallow D20, suggesting that climatic oscillations affect the population abundance and distribution of bigeye tuna.     

Protein and amino acid composition from the mantle of juvenile O ctopus vulgaris exposed to prolonged starvation

Protein and amino acid composition of the mantle of juvenile O ctopus vulgaris (Cuvier, 1797) during fasting for 27 days were determined. Average protein content of octopus mantle was of 711.19 ± 46.80 g kg^?1 DW, and it decreased with increasing fasting days. The non‐essential amino acids content was higher (486.18 ± 11.08 g kg^?1 protein) than essential amino acids (425.82 ± 9.15 g kg^?1 protein) at the start of the experiment (unstarved animals). The results suggest that the amino acid profile of the mantle where the most abundant amino acids are Arg, His, Lys, Gly, Leu and Pro could indicate a prolonged fasting condition (>20 days) or poor nutrition of O . vulgaris. This study supports the idea of using mantle for metabolic needs of starved O . vulgaris suggesting that the degradation pathway of amino acids to pyruvate and tricarboxylic acid cycle intermediates was favoured contrary to the degradation pathway of ketogenic amino acids. Special considerations should be taken concerning Thr, Ile, Ser, Ala, Asx (Asp, Asn), Glx (Glu, Gln) (because of their fast intake) and Lys and His (due to their stable contents) during a prolonged period of fasting.     

Growth performance,digestive enzyme activity and immune response of Japanese sea bass,Lateolabrax japonicus fed with fructooligosaccharide

In this experiment, a feeding trial was performed to determine the effects of fructooligosaccharide (FOS) on growth performance, digestive enzyme activity and immune response of Japanese sea bass, Lateolabrax japonicus juveniles (initial weight 38.3 ± 0.5 g), and the fish were examined following feeding with six levels of FOS (0, 0.5, 1, 2, 4 and 6 g/kg) for 28 days. Significant enhancement of weight gain (WG) and specific growth rate (SGR) was found in fish fed 1 g/kg FOS incorporated diets (p < .05), while the feed conversion ratio (FCR) in the 1, 2 g/kg FOS groups reduced significantly compared with the control (p < .05). Besides, the crude lipid in the 4, 6 g/kg FOS groups increased significantly compared with the control (p < .05). On the other hand, the erepsin and lipase activities significantly elevated in intestine of fish fed 2 g/kg FOS (p < .05) and the lysozyme activity in serum of fish fed 2 g/kg FOS were significantly higher than that in the control (p < .05). Moreover, the alkaline phosphatase activities in serum of fish fed 0.5, 1, 2 g/kg FOS were significantly higher than in control (p < .05). Regression analysis showed that the relationships between dietary FOS levels and either SGR, FCR, erepsin or lysozyme activities were best expressed by regression equations, and the optimal inclusion levels are 1.37, 1.80, 3.06, 3.11, 1.93 and 1.80 g/kg for SGR, FCR, erepsin, lipase, lysozyme and total superoxide dismutase activities, respectively. Overall, this study revealed that FOS incorporated diets could beneficial for L. japonicus culture in terms of increasing the growth, digestion and immune activities. Under the present experimental condition, the optimal supplementary level of FOS in the diet of L. japonicus is 1–3 g/kg.     

美人鱼发光杆菌对凡纳滨对虾非特异性 免疫功能及抗病力的影响

在基础饲料中分别添加0.1%和1%美人鱼发光杆菌灭活菌、0.1%美人鱼发光杆菌活菌配制成3种免疫实验饲料,以基础饲料为空白对照组饲料,每组设3个平行样。对个体质量为(4.83±0.36)g的凡纳滨对虾进行为期20 d的饲养实验,分别在0、5、10、15和20d进行取样,以血清中的酚氧化酶(PO)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、超氧化物歧化酶(SOD)和溶菌酶(UL)活性为免疫指标,探讨了美人鱼发光杆菌作为免疫制剂对凡纳滨对虾非特异性免疫效应的影响;在投喂免疫饲料后的第22天,按0.004 2 kg/kg体重的剂量,直接投喂对虾白斑综合征病毒(WSSV)病料,并记录累积死亡率。结果表明,美人鱼发光杆菌免疫实验组对凡纳滨对虾血清中PO、ACP、AKP、UL和SOD活性影响明显高于对照组,并且在饲料中添加美人鱼发光杆菌后,明显提高了对虾抵御WSSV感染的能力。其中0.1%美人鱼发光杆菌活菌实验组的抗病毒感染能力最强,WSSV感染14d内累计死亡率为63.3%±5.8%;而对照组为96.7%±3.3%。研究表明,美人鱼发光杆菌添加在对虾饲料中能提高凡纳滨对虾非特异性免疫水平,增强抵抗疾病的能力,将其作为对虾免疫增强剂具有良好的应用前景。     

Effects of Spirulina platensis as a feed additive on growth and coloration of blue dolphin cichlids (Cyrtocara moorii Boulunger, 1902)

This study was carried out to investigate the effects of replacing fish meal with dietary Spirulina as a feed supplement on the growth performance and coloration of blue dolphin cichlids (Cyrtocara moorii). Five isonitrogenous (47% crude protein) and isocaloric (17.36 kJ/g digestible energy) diets were for formulated to replace FM with 0, 5, 10, 15 and 20% Spirulina (designated as Control, SP5, SP10, SP15 and SP20 respectively) and fed to the fish (initial body weight, 3.15 ± 0.01 g). Fish were randomly distributed into fifteen 120 L aquariums (26.5 ± 1.00°C), 15 fish per aquarium. The diets were tested in triplicate for 12 weeks. Experimental groups were fed twice daily (09:00 and 17:00) by hand to satiation. At the end of the feeding trial, significantly (p < 0.05) higher weight gain (WG), specific growth rate (SGR), protein efficiency ratio (PER) and lower feed conversion ratio (FCR) were observed in fish fed the SP10 diet when compared to the SP20 diet. There was no significant difference in these parameters between the other groups. The skin coloration of blue dolphin cichlid fed a diet containing Spirulina meal was enhanced. The best coloration was observed in the SP15 group. These impressions were objectively validated by chemical determinations of carotenoids extracted from fish skins and passed statistical tests of significance. The study findings show that Spirulina meal does not diminish growth rates except at very high levels.     

Dietary thiamin and pyridoxine requirements of fingerling Indian major carp,Cirrhinus mrigala (Hamilton)

Two experiments were conducted to quantify the dietary thiamin (experiment I) and pyridoxine (experiment II) requirements of fingerling Cirrhinus mrigala for 16 weeks. In experiment I, dietary thiamin requirement was determined by feeding seven casein–gelatin‐based diets (400 g kg^?1 CP; 18.69 kJ g^?1 GE) with graded levels of thiamin (0, 0.5, 1, 2, 4, 8 and 16 mg kg^?1 diet) to triplicate groups of fish (6.15 ± 0.37 cm; 1.89 ± 0.12 g). Fish fed diet with 2 mg kg^?1 thiamin had highest specific growth rate (SGR), protein retention (PR), RNA/DNA ratio, haemoglobin (Hb), haematocrit (Hct), RBCs and best feed conversion ratio (FCR). However, highest liver thiamin concentration was recorded in fish fed 4 mg thiamin kg^?1 diet. Broken‐line analysis of SGR, PR and liver thiamin concentrations exhibited the thiamin requirement in the range of 1.79–3.34 mg kg^?1 diet (0.096–0.179 μg thiamin kJ^?1 gross energy). In experiment II, six casein–gelatin‐based diets (400 g kg^?1 CP; 18.69 kJ g^?1 GE) containing graded levels of pyridoxine (0, 2, 4, 6, 8 and 10 mg kg^?1 diet) were fed to triplicate groups of fish (6.35 ± 0.37 cm; 1.97 ± 0.12 g). Fish fed diet containing 6 mg kg^?1 pyridoxine showed best SGR, FCR, PR, RNA/DNA ratio, Hb, Hct and RBCs, whereas maximum liver pyridoxine concentration was recorded in fish fed 8 mg kg^?1 dietary pyridoxine. Broken‐line analysis of SGR, PR and liver pyridoxine concentrations reflected the pyridoxine requirement from 5.63 to 8.61 mg kg^?1 diet. Data generated during this study would be useful in formulating thiamin‐ and pyridoxine‐balanced feeds for the intensive culture of this fish.     

Sea bream culture in Egypt; status, constraints and potential

Marine fish farming in Egypt began in 1976 with the culture of gilthead sea bream, (Sparus aurata) as this fish was notably adaptable to brackish and marine pond conditions. Today, marine fish and shrimp farms amount to about 19,000 ha, out of which 42% is already in production while the rest, i.e., 58% is still under construction. In 1997, cultured gilthead sea bream production of 2,250 tons made up 3% of the 75,000 tons total aquaculture catch. In polyculture, usually with the grey mullet and sea bass, gilthead sea bream contributed 440 kg ha^–1 to the total yield of 1,700 kg ha^–1 (26%) over a period of 16 months. For the same period, the yield of monoculture ponds averaged 100 kg ha^–1, while in marine cages, yields ranged from 4–10 kg m³. In 1996–1997, fry of 0.25–1 g and fingerlings 1–10 g with a total of 3 million, were collected from the wild and 1 million fry were produced in the three marine hatcheries out of the four existing ones. The development of sea bream culture in Egypt is now severely inhibited by the shortage of seeds and adequate feeds. Exports of both sea bream and sea bass, during 1994–1996 averaged 1,300 tons per year.     

A Minchinia mercenariae‐like parasite infects cockles Cerastoderma edule in Galicia (NW Spain)

The cockle Cerastoderma edule fishery has traditionally been the most important shellfish species in terms of biomass in Galicia (NW Spain). In the course of a survey of the histopathological conditions affecting this species in the Ria of Arousa, a haplosporidan parasite that had not been observed in Galicia was detected in one of the most productive cockle beds of Galicia. Uni‐ and binucleate cells and multinucleate plasmodia were observed in the connective tissue mainly in the digestive area, gills and gonad. The parasite showed low prevalence, and it was not associated with abnormal cockle mortality. Molecular identification showed that this parasite was closely related to the haplosporidan Minchinia mercenariae that had been reported infecting hard clams Mercenaria mercenaria from the Atlantic coast of the United States. The molecular characterization of its SSU rDNA region allowed obtaining a fragment of 1,796 bp showing 98% homology with M. mercenariae parasite. Phylogenetic analysis supported this identification as this parasite was clustered in the same clade as M. mercenariae from the United States and other M. mercenariae‐like sequences from the UK, with bootstrap value of 99%. The occurrence of M. mercenariae‐like parasites infecting molluscs outside the United States is confirmed.     

Involvement of gonadal steroids in final oocyte maturation of white perch (Morone americana) and white bass (M. chrysops): in vivo and in vitro studies

Plasma estradiol-17 (E₂), testosterone (T), 17,20-dihydroxy-4-pregnen-3-one (DHP) and 17,20,21-tri-hydroxy-4-pregnen-3-one (20-S) levels were measured by radioimmunoassay (RIA) in white perch (Morone americana) and white bass (M. chrysops) that were induced to undergo final oocyte maturation (FOM) with human chorionic gonadotropin (hCG). Plasma DHP levels increased in females of both species in association with oocyte germinal vesicle migration (GVM) and germinal vesicle breakdown (GVBD) and decreased thereafter. Plasma 20-S levels also increased with oocyte GVM in white bass, but were several-fold lower than DHP levels. Circulating E₂ and T levels were greatest during GVM and GVBD in both species and decreased to low levels during oocyte hydration and ovulation. Follicles from white perch and white bass which received a priming injection of hCG in vivo, produced both DHP and 20-S in vitro after exposure to hCG and their oocytes underwent GVBD. Ovarian incubates from unprimed fish of either species produced only E₂ and T and their oocytes did not complete GVBD. Oocytes from unprimed bass, but not perch, matured when follicles were exposed to hCG in vitro. Both trilostane and cycloheximide blocked in vitro production of DHP and 20-S and oocyte GVBD by white perch follices. DHP and 20-S were equipotent inducers of FOM in the GVBD bioassay. None of several other structurally-related steroids tested were effective within a physiological range of concentrations. These results indicate a role for DHP and 20-S in the control of FOM in white perch and white bass.