Growth of the whole root system for a plant crop of sugarcane under rainfed and irrigated environments in Brazil

Sugarcane crops are managed over 8 million hectares in Brazil and future extensions might occur on less favorable lands where irrigation would be necessary to increase and stabilize yields. Root growth was studied by sequential soil coring under rainfed and irrigated conditions for one cultivar widely planted in Brazil. Root length densities (RLD) were measured 34, 49, 125, 179, 241 and 322 days after planting (DAP) down to a depth of 1 m. At the harvest (332 DAP), root intersects (a proxy for RLD) were counted on two vertical trench walls in each water supply regime, down to a depth of 6.0 m. The highest RLD in deep layers (below a depth of 0.6 m) were observed in the rainfed crop from 125 DAP onwards. By contrast, the highest RLD in the upper layers during dry periods were found in the irrigated crop. The maximum depth reached by roots at the harvest was little affected by irrigation: 4.70 m and 4.25 m in the rainfed and irrigated crop, respectively. About 50% of root intersects were observed below the depth of 1 m in the two water supply regimes. This pattern suggested a strong genetic control of root growth in deep soil layers. The total amount of root intersects 332 DAP was 49% higher in the rainfed crop than in the irrigated crop, and root distribution was more homogeneous. Mean root front velocity was about 0.5 cm day⁻¹ the first 4 months after planting and increased thereafter up to the end of the harvest (1.86 cm day⁻¹ and 1.75 cm day⁻¹ on average in the rainfed and the irrigated crops, respectively). Our study pointed out the necessity to take into account the development of sugarcane roots in deep soil layers to improve our understanding of net primary production control by water availability.     

Effects on rice plant morphology and physiology of water and associated management practices of the system of rice intensification and their implications for crop performance

Field experiments were conducted in Bhubaneswar, Orissa, India, during the dry season (January–May) in 2008 and 2009 to investigate whether practices of the System of Rice Intensification (SRI), including alternate wetting and drying (AWD) during the vegetative stage of plant growth, could improve rice plants’ morphology and physiology and what would be their impact on resulting crop performance, compared with currently recommended scientific management practices (SMP), including continuous flooding (CF) of paddies. With SRI practices, grain yield was increased by 48% in these trials at the same time, there was an average water saving of 22% compared with inundated SMP rice. Water productivity with AWD-SRI management practices was almost doubled (0.68 g l⁻¹) compared to CF-SMP (0.36 g l⁻¹). Significant improvements were observed in the morphology of SRI plants in terms of root growth, plant/culm height, tiller number per hill, tiller perimeter, leaf size and number, leaf area index (LAI), specific leaf weight (SLW), and open canopy structure. These phenotypic improvements of the AWD-SRI crop were accompanied by physiological changes: greater xylem exudation rate, crop growth rate, mean leaf elongation rate (LER), and higher light interception by the canopy compared to rice plants grown under CF-SMP. SRI plants showed delayed leaf senescence and greater light utilization, and they maintained higher photosynthetic rates during reproductive and grain-filling stages. This was responsible for improvement in yield-contributing characteristics and higher grain yield than from flooded rice with SMP. We conclude that SRI practices with AWD improve rice plants’ morphology, and this benefits physiological processes that result in higher grain yield and water productivity.     

Assessment of the long-term variation in the nitrogen pollution load potential from farmland to groundwater in the Tedori River Basin, Japan

The long-term nitrogen pollution load potential (NPLPg) to groundwater from farmlands was examined in the Tedori River Basin. The NPLPg was estimated using the difference between N in the fertilizer application rate and N outputs in crop yield at 5-yearly intervals from 1960 to 2005. The total yearly NPLPg of 1,085 t (103 kg) in the 1960s decreased to 774 t by 1975. Thereafter, the NPLPg gradually increased to 976 t in the 1990s, but decreased again to 369 t in 2005. The NPLPg decreased by 23% for rice and by 37% for horticultural crops from 1960 to 2005 with an overall decrease of 34%. The NPLPg per unit area was relatively stable over time for rice, soybean, barley, and horticultural crops, but there were significant differences among them. The NPLPg for rice ranged from 39 to 85 kg ha⁻¹ year⁻¹ with an average of 65 kg ha⁻¹ year⁻¹ and that of the horticultural crops ranged from 273 to 357 kg ha⁻¹ year⁻¹ with an average of 302 kg ha⁻¹ year⁻¹ The significant long-term changes in the NPLPg suggest that evaluation at a specific point in time is insufficient for an integrated assessment of groundwater pollution.     

Application of system of rice intensification practices in the arid environment of the Timbuktu region in Mali

Cereal production is chronically deficit in the Timbuktu region of Mali, sufficient for only 4.5 months of annual household consumption. Small-scale, village-based irrigation schemes, usually 30–35 ha in size, irrigated by a diesel motor pump, have become important to improve food security in this arid region. The NGO Africare has worked during the past 12 years with farmers in Goundam and Dire circles to establish irrigation schemes and provide them with technical assistance. In 2007, Africare undertook a first test of the System of Rice Intensification (SRI) in Goundam circle. After farmers observed a yield of 9 t ha⁻¹ of paddy compared to 6.7 t ha⁻¹ in the control plot there was interest in larger scale testing of the SRI system. In 2008, Africare, in collaboration with the local Government Agriculture Service and with support from the Better U Foundation, implemented a community-based evaluation of SRI with 60 farmers in 12 villages. Farmers in each village selected five volunteers, who each installed both SRI and control plots, side by side, starting the nurseries on the same day and using the same seed. For SRI plots, seedlings were transplanted one plant hill⁻¹ at the two-leaf stage (on average, 11.6 days old), with spacing of 25 cm × 25 cm between hills and aligned in both directions. This allowed farmers to cross-weed with a cono-weeder, on average 2.4 times during the season. In the control plots, farmers planted 3 plants hill⁻¹ with seedlings 29.4 days old and spaced on average 23.7 cm, not planted in lines. Weeding was done by hand. 13 t ha⁻¹ of organic matter was applied under SRI management, and 3 t ha⁻¹ in the control plots. Fertilizer use was reduced by 30% with SRI compared to the control. Although alternate wetting and drying irrigation is recommended for SRI, this was not optimally implemented due to constraints on irrigation management within the scheme; thus water savings were only 10% compared to the control. Average SRI yield for all farmers reached 9.1 t ha⁻¹, with the lowest being 5.4 t ha⁻¹ and highest being 12.4 t ha⁻¹. SRI yields were on average 66% higher than the control plots at 5.5 t ha⁻¹, and 87% higher than the yields in surrounding rice fields at 4.9 t ha⁻¹. Number of tillers and panicles hill⁻¹, number of tillers and panicles m⁻², and panicle length and number of grains panicle⁻¹ were clearly superior with SRI compared to control plants. Farmers tested five varieties, all of which produced better under SRI. The SRI system allowed for a seed reduction of 85–90%: from 40–60 kg ha⁻¹ for the control plots to 6.1 kg ha⁻¹ under SRI. Although production costs per hectare were 15% higher for SRI, revenue was 2.1 times higher than under the control. Farmers were very satisfied with these results. In 2009/2010, Africare and the Government’s agriculture service worked with over 270 farmers in 28 villages to scale up SRI practices and to test innovations, including composting techniques, optimization of irrigation, and techniques to reduce labor requirements and production costs. The good crop performance along with other advantages was confirmed in this third year with SRI yields of 7.7 t ha⁻¹ (n = 130 farmers) compared to 4.5 t ha⁻¹ in farmers’ fields.     

Fertilization management of paddy fields in Piedmont (NW Italy) and its effects on the soil and water quality

The fertilization management of the rice crop in Piedmont was analyzed at a regional scale, and the agronomic and environmental sustainability of the actual fertilization strategy of rice was evaluated through the analysis of its effect on the soils and waters quality. On average, a total amount of 127 kg ha⁻¹ of N, 67 kg ha⁻¹ of P₂O₅ and 161 kg ha⁻¹ of K₂O were supplied to the rice crop. In most cases N and P fertilization was rather well balanced with crop removal. The N balance was in the range ±50 kg for 77% of the surface. The low concentration of N in the groundwater reflected the small N surplus. P fertilization resulted to be smaller than removal for 53% of the surface. Nevertheless, the soil extractable P was very high, probably because of former higher P inputs. This resulted in a high concentration in water courses and aquifers. The K fertilization was excessive (surplus >100 kg ha⁻¹) for 53% of the surface, but most soils showed a low K content. K is probably contributing to nutrient leaching to a great extent. The average soil organic matter (SOM) content of paddy fields was higher than that of normally-cultivated soils in Piedmont, and the C/N was higher, owing to the low mineralization rate in waterlogged conditions. The SOM content was in relation with the management of the crop residues, as the tradition of burning straw after harvest was still widespread on 65% of the paddy surface.     

Stomatal resistance of three potato cultivars as influenced by soil water status, humidity and irradiance

Summary Leaf stomatal resistance varies considerably in response to changes in environmental conditions. Understanding cultivar differences in the response of stomata to these conditions is important for effectively simulating water-use and growth. In this study the stomatal resistance of three field grown potato (Solanum tuberosum L.) cultivars Atlantic. Monona and Norchip was observed in response to photosynthetic photon flux density, leaf to air vapour pressure difference and root zone available soil water. Substantial variation in stomatal resistance was accounted for by functions of the environmental variables for both Monona and Norchip. The explained variation for Atlantic was considerably less. Light saturation occurred near 500 μmol m⁻² s⁻¹. Increasing stomatal resistance above 1000 μmol m⁻²s⁻¹ was also observed but is likely an artifact of stomatal closure on days in which mid-day leaf water potentials dropped below critical levels. Although the leaf presumably insufficient to obtain substantial effects. The soil water status was partially linked to the average 1000 h to 1500 h stomatal resistance on clear, sunny days. Significant differences were observed among the cultivars in the response of stomata to changes in the available soil water.     

Assessment of long-term changes in nitrogen pollution load potential from sewage treatment water in the Tedori River Alluvial Fan Area, Japan

Long-term changes (1974–2007) in the nitrogen pollution load potential (NPLP) arising from sewage treatment water were assessed in the Tedori River Alluvial Fan Area of Japan. The total NPLP from sewage treatment systems (STS) during the 34 year period was 439 t (10³ kg) year⁻¹ from about 260,000 users in 1974 increasing to a peak of 793 t year⁻¹ in 1992 from about 363,000 users, and then decreasing to 676 t year⁻¹ from about 400,000 users in 2007. The NPLP outflow into the area increased from 356 t year⁻¹ in 1974 to a peak of 596 t year⁻¹ in 1985 followed by a rapid decrease to 98 t year⁻¹ in 2007. The NPLP outflow from the public STS to the Japan Sea began in 1979 and rapidly increased to 575 t year⁻¹ in 2007 from about 362,000 users. This represents 85.5% of the total NPLP. The NPLP from septic tanks in the area was 356 t year⁻¹ from about 107,000 users in 1974 gradually increasing to a peak of 587 t year⁻¹ from about 177,000 users in 1985 before rapidly decreasing to 60 t year⁻¹ from about 15,000 users in 2007. Although the current NPLP is about 98 t year⁻¹ in the study area, the average NPLP during 34 years was very different at 424 t year⁻¹. NPLP assessments affecting groundwater and closed water bodies should consider long-term processes of nitrogen pollution from STS over time periods compatible with the residence time of the receiving waters.     

Simulation of salt and water movement and estimation of water productivity of rice crop irrigated with saline water

The HYDRUS-ID model was experimentally tested for water balance and salt build up in soil under rice crop irrigated with different salinity water (ECiw) of 0.4, 2, 4, 6, 8 and 10 dS m⁻¹ in micro-lysimeters filled with sandy loam soil. Differences of means between measured (M) and HYDRUS-1D predicted (P) values of bottom flux (Q _o) and leachate EC as tested by paired t test were not found significant at P = 0.05 and a close agreement between RMSE values showed the applicability of the HYDRUS-1D to simulate percolation and salt concentration in the micro-lysimeters under rice crop. Potential ET values of rice as obtained from CROPWAT matched well with model predicted and measured one at all ECiw treatments. The model predicted root water uptake varied from 66.1 to 652.7 mm and the maximum daily salt concentration in the root zone was 0.46, 2.3, 4.5, 6.7, 8.4 and 10.2 me cm⁻³ in 0.4, 2, 4, 6, 8 and 10 dS m⁻¹ ECiw treatments, respectively. The grain production per unit evapotranspiration ( \textWP_{\textET\texta} {\text{WP}}_{{{\text{ET}}_{\text{a}} }} ) value of 2.56 in ECiw of 0.4 dS m⁻¹ treatment declined to 1.31 with ECiw of 2 dS m⁻¹. The \textWP_{\textET\texta} {\text{WP}}_{{{\text{ET}}_{\text{a}} }} reduced to one-fifth when percolation was included in the productivity determination. Similarly, the water productivity in respect of total dry matter production (TDM) was also reduced in different treatments. Therefore, the model predicted values of water balance can be effectively utilized to calculate the water productivity of rice crop.     

Site-specific nutrient management for intensive rice cropping systems in Asia

Irrigated rice (Oryza sativa L.) yield increases in Asia have slowed down in recent years. Further, yield increases are likely to occur in smaller increments through fine-tuning of crop management. On-farm experiments at 179 sites in eight key irrigated rice domains of Asia were conducted from 1997 to 1999 to evaluate a new approach for site-specific nutrient management (SSNM). Large variation in initial soil fertility characteristics and indigenous supply of N, P, and K was observed among the eight intensive rice domains as well as among farms within each domain. Field- and season-specific NPK applications were calculated by accounting for the indigenous nutrient supply, yield targets, and nutrient demand as a function of the interactions between N, P, and K. Nitrogen applications were fine-tuned based on season-specific rules and field-specific monitoring of crop N status. The performance of SSNM was tested for four successive rice crops. Average grain yield in the SSNM increased by 0.36 Mg ha⁻¹ (7%) compared to the current farmers’ fertilizer practice (FFP) measured in the same cropping seasons or 0.54 Mg ha⁻¹ (11%) compared to the baseline FFP yield before intervention. Average nutrient uptake under SSNM increased by about 10% in the same seasons or by 13% (N) and 21% (P, K) compared to the baseline data. Yield increases were associated with a 4% decrease in the average N rate, but larger amounts of fertilizer-K at sites where the previous K use was low. Average N use efficiencies increased by 30–40%, mainly through the use of improved in-season N management schemes. Across all sites and four successive rice crops, profitability increased by US$ 46 ha⁻¹ per crop or 12% of the total average net return. The performance of SSNM did not differ significantly between high-yielding and low-yielding climatic seasons, but improved over time with larger benefits observed in the second year. Average profitability increased from US$ 32 ha⁻¹ pre crop in the first year to US$ 61 ha⁻¹ pre crop in the second year due to improvements in the SSNM approach and re-capitalization of P and K applied in the first year. SSNM required little extra credit for financing, and remained profitable even if rice prices are somewhat lower than current levels. Further, scope for improvement exists at many sites by alleviating other crop management constraints to nutrient use efficiency. Profit increases ranged from US$ 4 to 82 ha⁻¹ per crop among eight rice domains. However, profit decreases occurred in about 25% of all cases, indicating that a certain minimum level of crop care is required for SSNM to be profitable. Yields at sites with labor-saving direct-seeding of larger fields were about 1 Mg ha⁻¹ lower than those achieved at sites with labor-intensive transplanting and good management, raising concern about future trends in rice production. SSNM has potential for improving yields and nutrient efficiency in irrigated rice to close existing yield gaps. The major challenge for SSNM will be to retain the success of the approach while reducing the complexity of the technology as it is disseminated to farmers. The nature of the approach will need to be tailored to specific circumstances in different countries. In some areas, SSNM may be field or farm specific, but in many areas it is likely to be just region and season-specific.     

Surface and subsurface losses of N and P from salt-affected rice paddy fields of Saemangeum reclaimed land in South Korea

The present study was carried out to evaluate nutrient losses that occur during the course of agricultural activity from rice paddy fields of reclaimed tidal flat. For this study, we chose a salt-affected rice paddy field located in the Saemangeum reclaimed tidal area, which is located on the western South Korean coasts. The plot size was 1,000 m² (40 m × 25 m) with three replicates. The soil belonged to the Gwanghwal series, i.e., it was of the coarse silty, mixed, mesic type of Typic Haplaquents (saline alluvial soil). The input quantities of nitrogen and phosphorus (as chemical fertilizer) into the experimental rice paddy field were 200 kg N ha⁻¹ and 51 kg P₂O₅ ha⁻¹ per annum, and the respective input quantities of each due to precipitation were 9.3–12.9 kg N ha⁻¹ and 0.4–0.7 kg P ha⁻¹ per annum. In terms of irrigation water, these input quantities were 4.5–8.2 kg N ha⁻¹ and 0.3–0.9 kg P ha⁻¹ per annum, respectively. Losses of these nutrients due to surface runoff were 22.5–38.1 kg N ha⁻¹ and 0.7–2.2 kg P ha⁻¹ for the year 2003, and 26.8–29.6 kg N ha⁻¹ and 1.6–1.9 kg P ha⁻¹ for the year 2004, respectively. Losses of these nutrients due to subsurface infiltration during the irrigation period were 0.44–0.67 kg N ha⁻¹ and 0.03–0.04 kg P ha⁻¹ for the year 2003, and 0.15–0.16 kg N ha⁻¹ and 0.05–0.06 kg P ha⁻¹ for 2004. When losses of nitrogen and phosphorus were compared to the amount of nutrients supplied by chemical fertilizers, it was found that 11.3–19.1% of nitrogen and 0.5–1.7% of phosphorus were lost via surface runoff, whereas subsurface losses accounted to 0.2–0.8% for nitrogen and only 0.02–0.04% for phosphorus during the 2-year study period.     

Diffusion of oxygen through lenticels in potato tuber

Summary Diffusion into the potato tuber of oxygen for respiration takes place only through lenticels, and not through the rest of the periderm. The number of lenticels which were capable of injection with methylene blue solution was observed to be about 100 per tuber. Rates of oxygen diffusion per lenticel varied from 0.024 to 0.296 cm³ h⁻¹ atm⁻¹ with a mean of 0.1105±0.013 cm³ h⁻¹ atm⁻¹.     

The effect of physiological age and planting date on the response of potatoes to applied nitrogen and on levels of residual nitrogen post-harvest

Summary Experiments were conducted on sandy and deep silty soils to assess the effect of physiological age of seed tubers and planting date on the response of potatoes to nitrogen fertiliser. Nitrogen optima commonly had large standard errors which made interpretation of the data difficult. On the sandy soil with cv. Record, early planting increased yield in two years but had no effect on mean N optimum (303 kg ha⁻¹, NOpt). Mean NOpt was less on the silty soil where the cv. Pentland Squire was grown, and was reduced from 273 kg ha⁻¹ to 241 kg ha⁻¹ when planting was delayed by 4 weeks. Physiological age had no effect on NOpt. On the sandy soil, residual soil mineral nitrogen (RSMN) increased by 0.05 kg ha⁻¹ per kg N fertiliser applied; on the silty soil the increase was four times as large. RSMN was larger following the late planted crop on the silty soil in one year.     

Recycling of rice straw to improve wheat yield and soil fertility and reduce atmospheric pollution

Burning of rice straw is a common practice in northwest India, where rice–wheat cropping system is extensively followed. The practice results in loss of nutrients, atmospheric pollution and emission of greenhouse gases. A field experiment was conducted at Indian Agricultural Research Institute, New Delhi, India during the rabi season (November to April) of 2002–2003 to evaluate the efficacy of the various modes of rice straw recycling in soil in improving yield and soil fertility and reducing not only carbon dioxide emission but also nitrous oxide (N₂O) emission. The treatment with no rice straw incorporation and application of recommended doses of fertilizer (120, 26 and 50 kg N, P and K ha⁻¹, respectively), gave the highest yield of wheat. Treatments with the incorporation of rice straw at 5 Mg ha⁻¹ with additional amount of inorganic N (60 kg N ha⁻¹) or inoculation of microbial culture had similar grain yields to that of the treatment with no straw incorporation. The lowest yield was recorded in the plots where rice straw was incorporated in soil without additional inorganic N and with manure application. All the treatments with rice straw incorporation had larger soil organic C despite the effect on the mineralisation of soil organic matter. Emission of N₂O was more when additional N was added with rice straw and secondary when straw was added to the soil because of higher microbial activity. The study showed that burning of rice straw could be avoided without affecting yield of wheat crop by incorporating rice straw in soil with an additional dose of inorganic N or microbial inoculation. However, the reduction of N₂O emission due to avoiding burning is in part counterbalanced by an increase in emission during the subsequent wheat cultivation.     

Structure and performance of fibers prepared from liquefied wood in phenol

The fibers from liquefied wood in phenol (WPFs) were spun by adding hexamethylenetetramine as synthetics and cured by soaking in solution containing hydrochloric acid and formaldehyde as main components. The chemical structure of WPFs remarkably changed from that of liquefied wood was identified by FT-IR spectrometer. WPFs with the average diameter of 27∼42 μm, tensile strength of 230∼356 MPa, and modulus of 15∼31 GPa were obtained using spinning speed of 0.72 μm min⁻¹, hydrochloric acid concentration of 18.5 %, heating rate of 10 °C h⁻¹, and curing time of 4 h. These WPFs showed a high thermal stability and a complex thermal decomposition process by TG(thermogravimetric) analysis. It was also found that the two obvious weight loss temperatures of WPFs were 510°C and 748°C.     

Influence of storage temperature on rate of potato tuber tissue infection caused byPhytophthora infestans (mont.) de bary estimated by digital image analysis

Summary Potato tubers were inoculated with two biotypes ofPhytophthora infestans then stored at 3,7, 10 and 15°C. Image analysis quantified average reflective intensity (ARI) of diseased tissue from cut surfaces of sample tubers. Tuber tissue infection and infection rate were measured by calculating Mean ARI of samples. Average tuber tissue infection and infection rate was minimal at 3°C (P.i.-US8 orP.i.-US1). Tuber tissue infection increased at temperatures >3°C, from 220 Mean ARI seven days after inoculation (dai) to 190–150 Mean ARI 50 dai (depending on cultivar and biotype ofP. infestans). Rate of tuber tissue infection caused byP.i.-US1 at 7°C was about zero in cv. Snowden but greater than −0.2 ARI day⁻¹ (cvs Russet Burbank and Superior). Rate of late blight infection in tuber tissue generally increased with temperature from −0.2 ARI day⁻¹ (at 7°C) in all cultivars to a maximum of −0.8 ARI day⁻¹ (10°C).     

The effect of increasing NaCl in irrigation water on growth,gas exchange and yield of tobacco Burley type

Despite the economic importance of tobacco, there is limited field study on the quantitative response of growth and yield to increasing soil salinity. The effects of irrigation with saline water on yield components of field-grown tobacco (Nicotiana tabacum L.) “Burley” type plants were studied over two growing seasons. Growth, dry matter partitioning and gas exchange were measured either in rainfed or fully irrigated plants growing in a clayey–sandy–loam soil. The four fully irrigated treatments received amounts of saline waters at 0.54, 2.5, 5.0 or 10 dS m⁻¹ electrical conductivity (EC_w) equal to crop evapotranspiration. In both years, the electrical conductivity of the saturation phase (EC_e) across the 0.6 m topsoil profile increased with increasing salinity of the irrigation water. Soil moisture was markedly lower in the rainfed treatment than in fully irrigated treatments. Different saline concentrations of irrigation water had virtually no effect on soil moisture. Carbon assimilation rate, stomatal conductance and water use efficiency of the saline treatments were lower than the fully irrigated plants at 0.54 dS m⁻¹ (NW treatment) in 1996, but not in 1997. Transpiration rates were unaffected by salinity in both years. The highest yield was produced by plants irrigated with good quality water. The number of leaves per unit land area was greater for the NW plants, whereas there were no differences between the other four treatments. Salinity decreased plant dry matter and height at harvest, increased dry matter partitioning into leaves and decreased that into stems in both years. Dry matter partitioning to leaves was also greater for the rainfed plants than for the NW plants. Tobacco plants grown under field conditions showed a maximum reduction of relative yield at the highest salinity level of only 31%. The threshold values (0.56 and 0.96 dS m⁻¹) and the EC_e at which a 10% yield reduction was obtained (3.12 and 2.55 dS m⁻¹) calculated from the linear model of response of relative yield to increasing EC_e were typical of moderately sensitive crops. The EC_e values at which 50% yield was reduced (13.34 and 8.91 dS m⁻¹) were indicative of moderate tolerance to salinity.     

Slow-growth in vitro conservation of potato germplasm at normal propagation temperature

Summary In-vitro conservation of microplants at 24±1 °C was investigated in six potato genotypes belonging to different maturity groups. Growth was controlled by using different concentrations of sucrose (20, 40 and 60 g 1⁻¹) alone or in combination with either mannitol (20 and 40 g 1⁻¹) or sorbitol (20 and 40 g 1⁻¹) in Murashige and Skoog medium. Maximum microplant survival (55.5–77.8%) after 12 months of storage was on medium supplemented with 20 g 1⁻¹ sucrose plus 40 g 1⁻¹ sorbitol. Microplants so conserved were in good to very good condition, without phenotypic abnormalities and had enough nodes for sub-culturing. Microplant survival and microplant condition were closely associated with each other but not with root growth. The success of conservation was unaffected by maturity group of the genotypes. This conservation approach at 24±1 °C can be an effective alternative to low temperature (6–8 °C) storage, especially in tropical and sub-tropical conditions, where the ambient temperatures in summer can reach 45–50°C.     

Spatial variation of infiltration rate and compactness in paddy fields

Percolation loss of water in rice fields is a major cause of low water use efficiency. Variation of infiltration rate and soil compactness in four paddy fields (with clay, silty clay, clay loam, and loam textures) was investigated in northern Iran. In each field, in longitudinal and transverse directions, points located 0.5, 2.5, 6.5, 12.5, … m from the bunds were selected and water infiltration rate and resistance to penetration of a pocket penetrometer were measured. The results showed that in clay soil, average final infiltration rate (f _c) in longitudinal direction, transverse direction, and center of the field was 0.216, 0.136, and 0.08 cm day⁻¹, respectively. The f _c for loamy soil was 2.77, 2.32, and 0.409 cm day⁻¹, respectively. Similar differences were observed in the other two soil textures. In general, effect of direction of the field for measuring infiltration rate was not statistically significant. Loam and clay loam soils, with resistance to penetration of 0.37 and 0.33 kg cm⁻², were not significantly different. But, clay and silty clay soils with resistance to penetration of 0.25 and 0.14 kg cm⁻² were significantly different (P < 0.05). Resistance to penetration of the penetrometer was not affected significantly (P < 0.05) by direction of measuring this parameter in the field. The conclusion is that if measured soil physical properties in a paddy field are going to be representative of the whole field, they should be measured at different locations, especially near the bunds. Another strategy for obtaining a representative infiltration rate or compactness for a paddy field is uniform puddling of the field.     

Critical water content and water stress coefficient of soybean (Glycine max [L.] Merr.) under deficit irrigation

The objective of this research was to investigate the critical water content (θ _c) and water stress coefficient (K _s) of soybean plant under deficit irrigation. This research was conducted in a plastic house at the University of Lampung, Sumatra in Indonesia from June to September 2000. The water deficit levels were 0–20%, 20–40%, 40–60%, 60–80%, and 80–100% of available water (AW) deficit, arranged in Randomized Completely Block (RCB) design with four replications. The results showed that the soybean plant started to experience stress from week IV within 40–60% of AW deficit. The fraction of total available water (TAW) that the crop can extract from the root zone without suffering water stress (p) was 0.5 and θ_c was 0.305 m³ m⁻³. The values of K _s at p=0.5 were 0.78, 0.86, 0.78, and 0.71 from week IV to week VII, respectively. The optimum yield of soybean plant with the highest yield efficiency was reached at 40–60% of AW deficit with an average K _s value of 0.78; this level of deficit irrigation could conserve about 10% of the irrigation. The optimum yield of soybean plant was 7.9 g/pot and crop water requirement was 372 mm.     

The nitrogen response of potato (Solanum tuberosum L.) in the field: nitrogen uptake and yield, harvest index and nitrogen concentration

Summary The response of potato to different rates of nitrogen supply ranging from 0–40 g m⁻² N was studied in five field experiments near Wageningen. NL (52⁰ North). In total two late potato cultivars and two sites were used during successive seasons. The results are summarized in a set of regression equations separately for total crop and tubers. The relation between nitrogen taken up (g m⁻²) in the total crop and total dry matter production (g m⁻²) could be described with the exponential equation: 1942–1900 * 0.93^X (r²=0.953, n=62). Nitrogen concentrations in the dry matter increased linearly with nitrogen uptake. Harvest indices for dry matter and nitrogen tended to decline with increase in N uptake. Cultivars differed only in the effect on N on tuber dry matter concentration. The relation between nitrogen uptake and nitrogen supply could be fitted with quadratic regression models. but coefficients were influenced by site and season.