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应用微卫星标记定位水稻恢复系密阳46的主效和微效恢复基因 总被引:8,自引:1,他引:7
应用由704个株系组成的珍汕97A/(珍汕97B/密阳46)F6测交群体,针对水稻第10染色体和第1、11染色体短臂构建了微卫星标记连锁图谱,检测到控制野败型细胞质雄性不育育性恢复的4个QTL,其中位于第10染色体长臂中下部的Rf4具有主效效应,位于第1染色体短臂的Rf3具有较大效应,位于第10染色体长臂近着丝粒处的qRf10和第11染色体短臂近着丝粒处的qRf11表现出微效作用。研究还表明,在主效基因Rf4存在时,其他3个基因仍具有提高结实率的作用,但在Rf3和Rf4同时存在时,qRf10和qRf11的效应不明显。 相似文献
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T优5570是福建农林大学作物学院与福建省种子总站合作以自育的T55A不育系为母本 ,明恢70为父本配组而成的杂交稻新组合。该组合不育系配合力突出、异交性好、育性稳定。用T55A与强恢复系明恢70配制的T优5570 ,具有制种纯度高、杂种优势强、丰产性好等特性。一、选育过程1.T55A的选育1992年早季在福州配制单交组合珍汕97B/地谷B ,秋季种植杂种一代。F1 抽穗时用龙特甫B进行复交。复交F1 选株建立株系 ,F4 代开始选株与珍汕97A测交。于抽穗期间 ,从农艺性状与特B相似的株系中选择单株与珍汕97A测交。F5 抽穗期间 ,从每个小区随机取样5… 相似文献
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籼稻体细胞无性系雄性不育变异及其表现(英文) 总被引:7,自引:0,他引:7
通过组织培养,于1984一1988年间,在9个品种(14次)中离体筛选到雄性不育突变件48个,其中发生在R1代的20个,R2代的28个。根据花粉败育之特点,可将这些突变体分为无花粉型及花粉败育型。发现起源于IR54的一体细胞无性系“54257”,在其R2代分离出雄性不育突变体,继之对它以各种品种进行测交,其杂种一代有的组合表现育性恢复,有的组合仍保持雄性不育。由此确证它属核质互作型雄性不育。该雄性不育突变体,就其恢保关系考察,初步认为其细胞质性质与野败型(WA型)类似。这是由体细胞无性变异产生核质互作型雄性不育的首例报道。 相似文献
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籼粳杂交稻早熟性的研究 总被引:4,自引:0,他引:4
1994年用粳稻广亲和品种02428、ZH157、ZH110 分别和珍汕97A杂交,从回交后代中选得同质广亲和恢复系02428R(珍汕97A/024283 F5),ZH157R(珍汕97A/ZH1572 F6)和ZH110R(珍汕97A/ZH1103 F3 )3份,将上述同质广亲和恢复系分别与籼稻WA 胞质不育系珍汕97A 、珍育29A、早显A、矮败型胞质不育系协青早A 杂交,分析F1 的生育期。1995年用02428R等广亲和恢复系和明恢63 等籼型恢复系与早显A 、协青早A杂交,分析F1 的生育期等性状。通过具有早熟效应的早显A不育系与不同恢复系杂交,发现早显A可显著缩短杂交稻组合的生育期,但对产量影响不大,说明选育具早熟效应的不育系对于缩短杂交水稻的生育期具有一定的价值。 相似文献
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为了探讨红莲型不育细胞质应用于杂交粳稻育种的可能性,利用包台(BT)型、红莲(HL)型、茶野(CL)型、野败(WA)型等4种细胞质的同核异质不育系六千辛A与181个粳稻亲本测交,根据测交F1的小穗育性,筛选HL型粳稻恢复系。在此基础上,对筛选出的HL型粳稻恢复系与更多HL型不育系复测,并进行不同细胞质杂交粳稻的比对试验。研究结果表明:1)HL型和CL型不育系的恢、保关系十分一致,测交F1小穗育性间的相关性达极显著水平。2)HL型和CL型粳稻不育系的可恢性虽不如BT型不育系,但明显优于WA型粳稻不育系;以测交F1小穗育性达到85%以上作为选择标准,从BT型恢复系和广亲和恢复系中筛选出25个HL型(CL型)粳稻恢复系。3)不育系的核背景对杂种育性有影响,HL型六千辛A可恢性最好,其次为HL型陵香A,HL型珍5A可恢性最差。4)与BT型不育系配制的杂种相比,HL型和CL型不育系配制的杂种育性稳定性相对较差。5)HL型不育系配制的杂种与BT型、WA型不育系配制的杂种在播种至抽穗历期、株高、产量及品质性状上均无明显差异;HL型不育系配制的杂交粳稻结实率正常,与BT型不育系配制的杂种无明显差异,明显高于WA型不育系配制的杂种。说明HL型不育细胞质应用于杂交粳稻育种是可行的。 相似文献
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LU Chao LIU Jian JIANG Jian-hua Caleb Manamik BRERIA TAN He-lin Masahiko ICHII HONG De-lin 《水稻科学》2013,20(3):191-199
The DNA fragments about 1 600 bp were amplified using random amplified polymorphism DNA (RAPD) primer OPA12 with the templates of mitochondrial DNA of Zhenshan 97A and Zhenshan 97B,and were sequenced.The nucleotide sequences and lengths of the fragments from Zhenshan 97A and Zhenshan 97B showed no difference.The precise length of the fragment was 1 588 bp.Sequence characterized amplification region (SCAR) primers were then developed to discriminate the cytoplasmic male sterile (CMS) lines and their maintainer lines.A specific 1 588 bp fragment could be amplified with SCAR primers,CHI19F2/CHI19R2 and CHI20F3/CHI23R3,in the mitochondrial DNA of Zhenshan 97A,but not Zhenshan 97B.Furthermore,the specific fragment could be also amplified from the total DNA from green leaf tissues of Zhenshan 97A with SCAR primers,but not Zhenshan 97B.With the corresponding primers,the specific fragment could also be amplified from the total DNA of green leaves of other two CMS lines with wild abortive type cytoplasm (CMS-WA),namely Zhenpin A and Tianfeng A,but not in their maintainer lines.Moreover,using total DNA as template,each of the four pairs of SCAR primers could also be used to amplify the 1 588 bp fragment in CMS-ID (Indonesia paddy type) line Ⅱ-32A,but not in II-32B,and the specific fragment was amplified from the DNA of both F1 and F2 seedlings of Shanyou 63.The results of detecting the genetic purity of a man-made mixture of the seeds of Zhenshan 97A using CHI20F3/CHI23R3 were completely consistent with the phenotypes.Taken together,these results indicated that the specific 1 588 bp-fragment amplified by CHI20F3/CHI23R3 was the unique amplification products of CMS mitochondrial DNA,and could be used to distinguish CMS-WA and CMS-ID lines from their corresponding maintainer lines at the seedling stage. 相似文献
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玉米PEPC 基因在籼型水稻保持系不同遗传背景下的效应及转PEPC基因后代的耐光氧化特性 总被引:2,自引:0,他引:2
分别以转PEPC Kitaake和Kitaake作父本,以4份不同来源的保持系珍汕97B、K17B、Ⅱ 32B和G2480B为母本构建F1,考查PEPC 的表达及其对光合性状和农艺性状的影响。导入PEPC 后,水稻净光合速率、气孔导度、胞间CO2浓度和PEPC酶活性均表现为增加,最突出的是其PEPC酶活性均成倍增加。蒸腾速率方面,只有K17B/PEPC 表现为下降。珍汕97B的净光合速率、气孔导度和PEPC酶活性一般配合力相对效应值均最大。在农艺性状上,G2480B/PEPC 的有效穗数、结实率和单株产量均大幅度提高,分别增加了31.46%、1.39倍和1.83倍,千粒重也增加了7.84%。珍汕97B/PEPC 由于穗变短,即使结实率和千粒重大幅度增加,减产仍达9.84%。G2480B的穗长、每穗粒数、结实率和单株产量一般配合力相对效应值均最大,而珍汕97B最小。在耐光氧化方面,转PEPC 基因Kitaake明显优于Kitaake。说明PEPC 单个基因对改善籼型三系保持系的光合性能和增加产量有较重要的作用。在供试的4份保持系中选用保持系宜考虑G2480B,PEPC 在此遗传背景下的表达丰度较高,提高结实率和单株产量等农艺性状的正效应最好。 相似文献
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CHEN Xiao-rong YANG Kong-song Fu Jun-ru ZHU Chang-lan PENG Xiao-song HE Xiao-peng HE Hao-hua 《水稻科学》2008,15(1)
Two populations of Dongxiang wild rice(Oryza rufipogon),Shuitaoshu and Dongtangshang,were crossed with five male sterile rice lines with different cytoplasmic backgrounds(B06S,Zhenshan 97A,Xieqingzao A,Zhong 9A,and Yuetai A),and the seed setting rate of the F1 was used to judge the fertility restoration ability in the Dongxiang wild rice.With P1.F1,P2,and F2 populations as materials,the Akaike's Information Criterion(AIC)was used to identify the major genes affecting quantitative traits,and when the major genes existed,the genetic effects of the major gene and the polygene and their genetic variance were estimated through segregation analysis.The seed setting rates of the F1 generation varied from 45.98%to 76.57%,suggesting that the Dongxiang wild rice had the fertility restoration ability.One major gone plus polygene mixed inheritance model was the most fitted genetic model for this trait in all the F2 populations.The heritability values of the major genes varied from 56.63%to 88.29%and those of the polygenes from 2.74%to 30.97%,and the total heritability values were from 63.1 7%to 94.01%.The major gone inheritance of the combination Zhong 9A/Dongtangshang was controlled by the additive effect without dominant effect,and the other nine combinations were by the completely dominant inheritance. 相似文献
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选用6种细胞质类型的10个雄性不育系(保持系)和10个恢复系,按不完全双列杂交配制100个组合,对不同细胞质类型杂交稻产量等8个农艺性状的杂种优势进行了分析。结果表明,同一性状不同组合和同一组合不同性状间,同一细胞质类型(恢复系)和不同恢复系(母细胞质类型)所配系列组合间的超亲优势和对照优势存在着较大差异;每穗总粒数、每穗实粒数和穗长具有较强的正向超亲优势和对照优势,结实率具有较强的对照优势,产量性状也具有一定的超亲优势和对照优势,其它性状超亲优势和对照优势都比较弱;6种细胞质类型中以冈型和D型所配系列组合超亲优势和对照优势最强;10个恢复系中以R 388所配系列组合的超亲优势最强,密阳46次之,明恢63所配系列组合的对照优势最强,R 478次之。在大多数性状上恢复系对组合的超亲优势和对照优势的影响大于细胞质类型。 相似文献
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水稻亚种间杂种配子育性的研究 总被引:8,自引:1,他引:8
水稻亚种间杂种配子育性的研究何光华,郑家奎,阴国大,杨正林(四川省农业科学院水稻高粱研究所泸州646100)关键词亚种间杂种,雄性不育,雌性不育GameFertilityofF_1betweenIndicaandJaponica¥HEGuanghua... 相似文献
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CHEN Xiao-rong YANG Kong-song FU Jun-ru ZHU Chang-lan PENG Xiao-song HE Xiao-peng HE Hao-hua 《水稻科学》2008,15(1):21-28
Two populations of Dongxiang wild rice (Oryza rufipogon), Shuitaoshu and Dongtangshang, were crossed with five male sterile rice lines with different cytoplasmic backgrounds (B06S, Zhenshan 97A, Xieqingzao A, Zhong 9A, and Yuetai A), and the seed setting rate of the F1 was used to judge the fertility restoration ability in the Dongxiang wild rice. With P1, F1, P2, and F2 populations as materials, the Akaike's Information Criterion (AIC) was used to identify the major genes affecting quantitative traits, and when the major genes existed, the genetic effects of the major gene and the polygene and their genetic variance were estimated through segregation analysis. The seed setting rates of the F1 generation varied from 45.98% to 76.57%, suggesting that the Dongxiang wild rice had the fertility restoration ability. One major gene plus polygene mixed inheritance model was the most fitted genetic model for this trait in all the F2 populations. The heritability values of the major genes varied from 56.63% to 88.29% and those of the polygenes from 2.74% to 30.97%, and the total heritability values were from 63.17% to 94.01%. The major gene inheritance of the combination Zhong 9A/Dongtangshang was controlled by the additive effect without dominant effect, and the other nine combinations were by the completely dominant inheritance. 相似文献
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水稻雄性不育性在异源胞质背景的遗传表达 总被引:5,自引:0,他引:5
以早籼金早6号天然雄性不育株与金早6号正常可育株杂交F1为父本,9个水稻种质O. barthii、O. glaberrima、 O. sativa的印度春籼T141、非洲栽培稻Gambiaka Kokum、中国早籼珍汕97、印尼爪哇稻Boak和Gannal品种、中国粳稻花壳糯和日本粳稻南粳56 与早籼珍汕97杂交并多次回交的同核异质代换系为母本进行杂交,观察后代的遗传表现。杂交F2代结果表明,金早6号雄性不育性在9个异源胞质背景下全部分离并出现雄性不育株,未发现与金早6号雄性不育基因互作导致可育的胞质类型。 相似文献
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杂交粳稻同核异质不育系细胞质效应的研究 总被引:14,自引:1,他引:13
以具有ChinsurahBoroⅡ(BT)、滇型(D)、野败型(WA)和野栽型(WC)4种不育细胞质的不育系南粳11A和南粳34A及其相应保持系与77302-1、宁恢3-2、C57和C堡4个恢复系配制的杂种F1为材料,探讨了不育系细胞质对F1代12个性状的遗传效应。不育系细胞质对F1代的株高、穗颈长、每稳实粒数、结实率、收获指数和单株产量等性状有明显的负效应,而对稳数、每穗总粒数、千粒重和生物产量则有明显的正效应。不育系细胞质的这种遗传效应随细胞质来源的不同表现明显的差异,D型和BT型细胞质在多数性状上的表现优于WA型和WC型细胞质。此外,不育系细胞质与保持系细胞核和恢复系细胞核及三者之间的互作在多数性状上也有明显差异。 相似文献