共查询到20条相似文献,搜索用时 125 毫秒
1.
2.
3.
对松材线虫 7个株系和拟松材线虫 2个株系的线粒体DNA随机扩增多态性分析结果显示 ,松材线虫和拟松材线虫种间和种内都表现出很大的变异性 ,但种间变异明显大于种内变异 ,因而支持将松材线虫和拟松材线虫划分为两个不同的种。两个拟松材线虫株系 (法国株系和日本株系 )之间的相似值和松材线虫各株系间的相似值极为接近 ,由此看来 ,这两个株系的变异为种内变异 ,应同属于一个种。聚类分析的结果表明 ,松材线虫和拟松材线虫系两个明显不同的类群 ,即松材线虫类群和拟松材线虫类群。松材线虫类群又分为两个亚组 ,一个是以日本株系为代表 ,另一个是以北美洲 (美国和加拿大 )株系为代表 相似文献
4.
5.
对松材线虫7个株系和拟松材线虫2个株系的线粒体DNA随机扩增多态性分析结果显示,松材线虫和拟松材线虫种间和种内都表现出很大的变异性,但种间变异明显大于种内变异,因而支持将松材线虫和拟松材线虫划分为两个不同的种.两个拟松材线虫株系(法国株系和日本株系)之间的相似值和松材线虫各株系间的相似值极为接近,由此看来,这两个株系的变异为种内变异,应同属于一个种.聚类分析的结果表明,松材线虫和拟松材线虫系两个明显不同的类群,即松材线虫类群和拟松材线虫类群.松材线虫类群又分为两个亚组,一个是以日本株系为代表,另一个是以北美洲(美国和加拿大)株系为代表. 相似文献
6.
测定了菊科3种入侵植物乙醇提取物对松材线虫的生物活性。结果表明:飞机草、紫茎泽兰和薇甘菊以1.5g/L处理后72h校正死亡率分别为85.6%、87.6%和74.6%;飞机草、紫茎泽兰和薇甘菊乙醇提取液处理72h后的半致死质量浓度C50分别为0.6892、0.6813、0.7474g/L。即3种入侵植物乙醇提取物对松材线虫毒杀活性顺序强弱依次为紫茎泽兰>飞机草>薇甘菊。 相似文献
7.
基于TaqMan实时荧光PCR的拟松材线虫分子鉴定技术 总被引:1,自引:0,他引:1
《中国森林病虫》2021,(5)
拟松材线虫与松材线虫的亲缘关系极为接近,是松材线虫病病原鉴定的主要干扰物种。为了排除松材线虫病病原鉴定过程中拟松材线虫的干扰,采用TaqMan实时荧光PCR技术,以核糖体DNA内转录间隔区(ITS)为模板设计拟松材线虫特异性引物和TaqMan探针,开展拟松材线虫分子检测和鉴定技术研究。结果表明:拟松材线虫ITS2区域存在较多的碱基差异,以此为模板设计的拟松材线虫引物和TaqMan探针组合具有较强的特异性;该组合灵敏性高,能够实现对拟松材线虫单条线虫甚至单个卵的分子检测和鉴定。结合现有的松材线虫分子鉴定技术,可以为进一步构建松材线虫双重检测技术体系奠定基础,为林业和海关检疫提供可靠的技术支撑。 相似文献
8.
9.
本文对浙江省宁波市松材线虫病疫区内的松材线虫和拟松材线虫的耐久型四龄幼虫(LⅣ)的形态及其分化变异作了观察研究.结果表明,松墨天牛成虫体内携带的松材线虫和拟松材线虫的LⅣ在形态上完全相同;同一来源的(拟)松材线虫的LⅣ既可发育形成松材线虫,也可形成拟松材线虫.它的分化变异结果受寄主等环境因子影响,规律是:带尾尖突的LⅣ离开松墨天牛成虫进入黑松多变为无尾尖突的松材线虫;进入马尾松多变为带尾尖突的拟松材线虫. 相似文献
10.
《湖南林业科技》2007,34(1):62-62
2006年5月,由南京林业大学森林资源与环境学院叶建仁教授主持完成的“松材线虫SCAR标记与系列分子检测技术及试剂盒研制”通过了江苏省科技厅组织的科技成果鉴定。该项研究对松材线虫与拟松材线虫特异片段进行了系统筛选,共获得了7个松材线虫DNA特异片段与5个拟松材线虫DNA特异片段,为松材线虫与拟松材线虫的分子鉴定奠定了基础。首次将2个松材线虫与2个拟松材线虫的DNA特异片段成功转化为SCAR标记,丰富了松材线虫与拟松材线虫分子标记方法。首次采用SCAR标记方法成功构建了松材线虫检测试剂盒,PCR检测过程仅需2.2小时。首次成功标记了一个可用于检测松材线虫的非放射性探针DIG-F2/R1。用该探针对线虫基因组DNA点杂交,松材线虫均表现有较强的杂交信号,而拟松材线虫、霍夫曼尼伞滑刃线虫、大核滑刃线虫、长尾属线虫等均无杂交信号。 相似文献
11.
Successive chloroform, methanol and water extracts of bark and leaves of Albizzia lebbeck were tested for its in vitro mast cell stabilizing effect against compound 48/80. Methanolic extract of leaf and methanolic and water extracts of bark have shown maximum activity comparable to that of disodium chromoglycate. 相似文献
12.
13.
14.
In order to study the changes in ectomycorrhizal development during symptom expression of pine wilt disease, root window observations were conducted concurrent with measurements of leaf water potential as well as photosynthetic and transpiration rates of 5‐year‐old Pinus thunbergii trees that were inoculated with the pinewood nematode (PWN) Bursaphelenchus xylophilus. Infected trees were compared with girdled and uninfected control trees. Ectomycorrhizas developed constantly during the experimental period in control trees but did not develop in the girdled trees. Ectomycorrhizal development ceased within 2 weeks in those trees that finally died after PWN infection. In the trees that survived PWN infection, ectomycorrhizal development ceased within 1–4 weeks of inoculation but was resumed thereafter within 3–6 weeks. Ectomycorrhizal development ceased prior to a decrease in both photosynthetic rate and leaf water potential in the inoculated trees. 相似文献
15.
Christian Ulrichs Inga Mewis Sujit Adhikary Atanu Bhattacharyya Arunava Goswami 《Journal of pest science》2008,81(2):79-84
Antifeeding and toxic effects of chemical extracts from Porteresia coarctata Takeoka on the insect pest Spodoptera litura (F.) were investigated in the laboratory. Hexane extracts of P. coarctata leaves were re-dissolved in dimethyl sulfoxide and applied in water to leaves of Ricinus communis L. (castor). A concentration of 1,000 and 2,000 ppm extract exhibited significant antifeeding and toxic effects to third
instar larvae when compared to the same concentration of dimethyl sulfoxide in water as control. One day after feeding on
extract-treated castor leaves, the mortality of S. litura larvae was 50%. Mortality increased to 95% after 11 days at a dosage of 2,000 ppm. Compared to the control, third instar
larvae fed on castor leaves treated with P. coarctata leaf extract at different concentrations showed significant reduction in protein and DNA content in the fat body and midgut
tissues. Results demonstrate the insecticidal and antifeedant properties of P. coarctata leaf extract against the generalist pest S. litura leading to biochemical changes in the insect body. Thus, P. coarctata contains phytochemicals, and the extract, in crude and pure form, has potential as a botanical insecticide in alternative
control strategies against Lepidoptera pests. 相似文献
16.
17.
几种树种水浸液对杉木叶片硝酸还原酶的效应 总被引:1,自引:0,他引:1
采用完全随机区组设计,用马尾松(Pinusmassoniana)、木荷(Schimasuperba)、赤楠(Syzygiubuxifolium)、杉木(Cunninghamialanceolata)的枝叶1∶6浓度水浸液和清水共5个不同处理浇灌盆栽的杉木幼苗,2a后对其叶片的硝酸还原酶活性进行测定。结果表明,经过几种杉木伴生种水浸液处理2a后杉木的硝酸还原酶活性比杉木水浸液及清水处理的有较大程度的提高,其中经过马尾松水浸液处理的杉木叶片的硝酸还原酶活性最高,而经杉木枝叶水浸液处理的硝酸还原酶活性最低。 相似文献
18.
19.
Water extracts, methanolic extracts and the petrol ether phase from the fruit and leaves ofMedia azedarach L. were tested against different instar nymphs ofLocusta migratoria migratorioides (R. & F.) under laboratory conditions. Water extracts caused various degrees of mortality and a significant delay in the development and growth of the locusts. Treatments with 3% concn of water extracts from the fruit and leaves at a rate of 40ml/m2 gave 93% and 100% mortalities, respectively, of first instars, and a 3% concn of leaf extract resulted in a mortality of 93.2% of third instars. Antifeedant and/or stomach-poison effect(s) played an important role. MeOH extract had the same efficacy as water extracts, but the petrol ether phase did not show much effect on the locusts. 相似文献
20.
一种利用黑松枝条筛选杀松材线虫剂的生测方法 总被引:1,自引:0,他引:1
该文介绍了一种利用黑松枝条进行化合物对松材线虫作用的生测方法。通过研究枝条的接种线虫数量、含水量以及灭菌处理后对松材线虫繁殖的影响,确定了合适的生测条件,并对几种生测方法的优缺点进行了讨论。 相似文献