共查询到19条相似文献,搜索用时 125 毫秒
1.
植物生物反应器生产口服疫苗的研究进展 总被引:1,自引:0,他引:1
随着基因工程技术的快速发展、植物转基因技术的日趋成熟,植物已成为基因重组生物制品的重要表达载体。利用转基因技术构建植物生物反应器生产口服疫苗是目前新兴的研究领域。综述了亚单位抗原在转基因植物中的表达系统、转基因植物生产的重组疫苗、转基因植物疫苗存在的问题及解决方法等,并展望了其研究趋势和应用前景。 相似文献
2.
转基因植物生产口服疫苗的研究进展 总被引:1,自引:0,他引:1
概述了利用转基因植物生产口服疫苗的研究现状。分别对转基因植物生产口服疫苗的优点、作用原理、研究方法、已研究的口服疫苗及问题和前景进行了介绍。 相似文献
3.
植物基因工程疫苗的研究进展 总被引:1,自引:0,他引:1
植物基因工程疫苗是利用植物表达重组抗原蛋白来生产疫苗。作阐述了用转基因植物生产疫苗的基本原理和方法以及植物疫苗的优点,特别是食用疫苗的研究进展、优缺点及其应用前景。 相似文献
4.
融合保护性抗原的LT类全毒素具有传输抗原载体和免疫佐剂两重功效。利用基因工程技术,构建了带有信号肽的Pr1as-K88ac-STII-LTA 2/UP-LTB融合基因的双价植物表达载体。双价植物表达载体通过冻融法转化根癌农杆菌GV3101,并利用农杆菌介导技术叶盘法转化模式植物烟草。转基因烟草经PCR和RT-PCR初步检测,证明外源基因已经整合到受体植物基因组中,同时表明融合基因在转录水平上有表达。转基因烟草的获得,为进一步研制新型的抗腹泻植物口服疫苗奠定了良好的基础。 相似文献
5.
6.
转基因植物疫苗是将植物基因工程技术与机体免疫机理相结合,把外源基因引入植物体内,产生能使人体获得特异免疫能力的新疫苗,和其它疫苗相比,具有廉价、安全、有效等优点。在最近的研究中,越来越多的抗原蛋白在植物中得到了表达,进入了动物和体实验。在此,就这些进展以及植物疫苗的安全性进行了综述。 相似文献
7.
8.
9.
墨西哥科研人员通过培檀转基因植物,研制出一些新型口服疫苗。这些疫苗有望用来预防胃病、乙肝、霍乱和疟疾等疾病,并可能对预防癌症也有一定的效果。科研人员把不同疾病的致病基因植入西红柿或香蕉的植物胚胎中,并让胚胎在试管里生长至5厘米,然后移植到肥沃的土壤中,以传统方法培育。成熟的果实含有抗原蛋白。将这种有效成分提取出来就可制成针对不同疾病的口服疫苗。 相似文献
10.
11.
Transformation of Two VP1 Genes of O- and Asia 1-Type Foot-and-Mouth Disease Virus into Maize 总被引:1,自引:0,他引:1
ZHANG Su-zhi ZHANG Gui-ling RONG Ting-zhao PAN Li ZHOU Peng ZHANG Yong-guang 《中国农业科学(英文版)》2011,10(5):661-667
The expression of antigens in transgenic plants has increasingly been used as an alternative to the classical methodologies for the development of experimental vaccines. This paper reports here the development of a novel oral immunization system for foot-and-mouth disease (FMD) in transgenic maize with two serotypes of the structural protein VP1 of the footand-mouth disease virus (FMDV) viz., O- and Asia l-type, respectively. The transgenic plantlets were identified and investigated by polymerase chain reaction (PCR), Southern blot, and real-time PCR. Moreover, it was found that the VP1 genes in transgenic plants could be transmitted stably to the next generation through PCR detection. To our knowledge,this is the first report in an attempt to induce a protective systemic antibody response in animals by feeding the transgenic plants in which two serotypes antigen protein of FMDV expressed together. Results of the experiment provide the possibility of using plant-based vaccines as feedstuff or feedstuff additives. 相似文献
12.
Verez-Bencomo V Fernández-Santana V Hardy E Toledo ME Rodríguez MC Heynngnezz L Rodriguez A Baly A Herrera L Izquierdo M Villar A Valdés Y Cosme K Deler ML Montane M Garcia E Ramos A Aguilar A Medina E Toraño G Sosa I Hernandez I Martínez R Muzachio A Carmenates A Costa L Cardoso F Campa C Diaz M Roy R 《Science (New York, N.Y.)》2004,305(5683):522-525
Glycoconjugate vaccines provide effective prophylaxis against bacterial infections. To date, however, no commercial vaccine has been available in which the key carbohydrate antigens are produced synthetically. We describe the large-scale synthesis, pharmaceutical development, and clinical evaluation of a conjugate vaccine composed of a synthetic capsular polysaccharide antigen of Haemophilus influenzae type b (Hib). The vaccine was evaluated in clinical trials in Cuba and showed long-term protective antibody titers that compared favorably to licensed products prepared with the Hib polysaccharide extracted from bacteria. This demonstrates that access to synthetic complex carbohydrate-based vaccines is feasible and provides a basis for further development of similar approaches for other human pathogens. 相似文献
13.
14.
A A Mahmoud 《Science (New York, N.Y.)》1989,246(4933):1015-1022
Parasitic protozoans and helminths pose considerable medical as well as scientific challenges. Investigations of the complex and very different life cycles of these organisms, their adaptation to the obligate parasitic mode of life, and their ability to face the hostile host environment have resulted in many exciting discoveries. Invasion of host erythrocytes by plasmodial sporozoites and intact skin by schistosomal cercariae are outlined as examples of the elaborate mechanisms of parasitism. Isolation and characterization of single protective antigens or subunit vaccines from these two organisms are examined as models for vaccine development. Finally, developments in exploring gene regulation in protozoans and free and parasitic nematodes are briefly outlined. 相似文献
15.
核酸疫苗的研究进展及其在鱼类免疫中的应用 总被引:1,自引:0,他引:1
核酸疫苗是20世纪90年代初发展起来的一种新型疫苗,它克服了传统疫苗所存在的一些缺陷,具有许多传统疫苗不可比拟的优点。有关核酸疫苗的研究已成为国内外科技界研究的热点之一,呈现出良好的应用前景。作者综述了核酸疫苗的研究背景、构建过程、作用机理以及疫苗本身具有的优缺点等,同时,概述了近年来国内外对鱼类DNA疫苗基础研究和应用研究的新进展,探讨了鱼用DNA疫苗当前需要解决的问题及发展前景。 相似文献
16.
M F Good L H Miller S Kumar I A Quakyi D Keister J H Adams B Moss J A Berzofsky R Carter 《Science (New York, N.Y.)》1988,242(4878):574-577
Current vaccine development strategies for malaria depend on widespread immunological responsiveness to candidate antigens such as the zygote surface antigens and the sporozoite coat protein, the circumsporozoite (CS) protein. Since immunological responsiveness is controlled mainly by genes mapping within the major histocompatibility complex (MHC), the humoral immune response to the zygote surface antigens and the cytotoxic T lymphocyte (CTL) response to the CS protein were examined in MHC-disparate congenic mouse strains. Only two of six strains responded to the 230-kilodalton zygote surface antigen and another two strains responded to the 48/45-kilodalton surface antigen. From two mouse strains, expressing between them five different class I MHC molecules, there was recognition of only a single CTL epitope from the CS protein, which was from a polymorphic segment of the molecule. The restricted CTL response to this protein parallels the restricted antibody response to this protein observed in humans and mice. These findings suggest that subunit malaria vaccines now being developed may be ineffective. 相似文献
17.
18.
狂犬病是由狂犬病病毒感染中枢神经系统引起的一种古老的人兽共患传染病,人和动物感染后一旦出现临床症状,死亡率几乎100%,至今仍无有效的治疗方法。当前,接种疫苗是预防狂犬病最为有效的途径。因此,狂犬病疫苗研发一直是狂犬病研究领域的热点之一,进而不断涌现出新型疫苗。本文对近期狂犬病新型灭活疫苗、弱毒疫苗、核酸疫苗、亚单位疫苗、病毒样颗粒疫苗、口服疫苗等基因工程疫苗研究进展进行系统梳理,以期把握狂犬病疫苗研究现状,为研发更为有效的狂犬病新型疫苗提供新思路。 相似文献
19.
家禽养殖业是畜牧业的基础性产业,我国家禽年出栏量达 146 亿羽,约占全球养殖量的 22%,而鸡球虫病是一种严重危害养禽业健康发展的寄生性原虫病,每年给养鸡业造成巨大的经济损失。该病由一种或多种艾美耳球虫寄生于鸡肠道粘膜上皮细胞引起,可导致大量肠上皮细胞受损、出血甚至死亡。近 70 年来,国内外学者一直致力于鸡球虫病防治药物和疫苗的研制,传统的抗球虫药物有聚醚类离子载体抗生素和化学合成类药物,传统的鸡球虫病疫苗有强毒活卵囊疫苗和弱毒活卵囊疫苗。近年来,新药与新型疫苗研发技术平台不断发展,为新型抗球虫药物(天然产物药物)和新型疫苗(亚单位疫苗、DNA 疫苗和活载体疫苗等)的研制提供新的手段和思路。结合鸡球虫病流行病学、防治药物等方面的研究概况,重点阐述抗球虫药物与鸡球虫病疫苗研发等方面的研究进展,以期为鸡球虫病的综合防控提供理论依据。 相似文献