三峡库区小流域旱坡地氮磷流失特征研究

为探讨三峡库区小流域旱坡地氮磷流失特征,采用标准径流小区对重庆市涪陵区珍溪镇王家沟小流域常规耕作方式(顺坡耕作)以及水土保持耕作方式(常规顺坡耕作+PAM土壤调节剂、免耕+顺坡种植+稻草覆盖、常规顺坡耕作+植物篱、免耕+横坡垄作、常规横坡耕作)下土壤氮素和磷素的流失量及形态特征进行分析。结果表明:(1)就旱坡地土壤氮素的流失特征而言,径流中氮素的流失量相对较小,最大值仅为53.63mg/m2;而泥沙中所携带的氮素流失量相对较大,其最大值达131.25mg/m2;整个观测期内,氮素的流失主要以颗粒态为主,颗粒态氮的流失量占总氮流失量的53.0%～62.0%。(2)就旱坡地土壤磷素的流失特征而言,各处理径流中磷素的总体规律基本表现为:常规顺坡耕作>常规横坡耕作>免耕+顺坡种植+稻草覆盖>常规顺坡耕作+PAM土壤调节剂>免耕+横坡垄作>常规顺坡耕作+植物篱;泥沙中所携带的磷素流失量相对于径流中的磷素流失量而言较大,最大值为58.14mg/m2;整个观测期内,颗粒态总磷流失量为水溶态总磷流失量的1.75～2.15倍,流失方式同样以颗粒态为主。总体而言,水土保持耕作方式下的氮磷流失量均小于常规耕作处理,能有效减少径流及泥沙中各形态氮磷的流失。     

水土保持措施对径流泥沙及养分流失的影响

以北京市门头沟区龙凤岭水土保持科技示范园为研究对象,对2005—2009年汛期(5—11月)径流小区的径流量、侵蚀产沙量及养分流失量进行了分析。结果是:同为10 m坡长的径流小区,其径流量和养分流失量从大到小依次为裸地、坡耕地和石坎梯田,产沙量最大的是裸地,其次是石坎梯田,最小的是坡耕地;与裸地小区相比,10 m坡长的水土保持措施小区可减少全氮流失量50.5%~91.4%、减少全磷流失量31.8%~75.8%;20 m坡长裸地径流小区的径流量、泥沙量和养分流失量均明显大于鱼鳞坑,鱼鳞坑小区可减少径流量68.4%~83.0%、减少产沙量71.1%~82.7%、减少全氮流失量64.4%~74.9%、减少全磷流失量40.6%~87.3%。说明水土保持措施具有良好的保持水土和减少养分流失的作用。     

耕作措施及雨强对南方红壤坡耕地侵蚀的影响

选择典型南方红壤区平均坡度为10°的坡耕地小区进行天然降雨观测,对横坡耕作、顺坡耕作、顺坡耕作+植物篱、稻草覆盖4种耕作措施在侵蚀过程中的径流泥沙和养分流失特征进行研究。结果表明,监测期间,径流深和泥沙流失量基本随雨强的增大而增加。随着耕作措施由顺坡耕作向顺坡耕作+植物篱、横坡耕作、稻草覆盖的转换,减流效益、减氮效益和减磷效益依次增大。稻草覆盖措施减流效益最佳,为91.77%;横坡耕作措施减沙效益最佳,为98.91%;稻草覆盖、横坡耕作和植物篱3种措施在防治高强度降雨引发的土壤侵蚀和养分流失具有较高的效益。耕作措施对泥沙粒径分布有影响。与顺坡耕作、横坡耕作相比,植物篱和稻草覆盖措施能够更有效地拦截径流中的粗颗粒。该研究可为南方红壤丘陵区坡耕地选择合适的耕作措施和防治农业非点源污染提供依据。     

不同土地利用方式下片麻岩坡面养分流失规律的研究

通过室内模拟人工降雨试验,研究不同土地利用方式片麻岩坡面水土流失和养分流失特征。结果表明:谷子地、草地坡面产沙量产流量远小于裸地产沙产流量;不同土地利用方式片麻岩坡面产流产沙变化过程不同,草地、谷子地达到稳定产流的时间滞后于裸地。3种土地利用方式坡面养分流失随时间的变化过程基本相同,降雨起始各种养分浓度较高,随着时间的持续,养分流失浓度趋于稳定。不同土地利用方式下片麻岩坡面养分流失量表现为钾流失总量 > 氮流失总量 > 磷流失总量,养分流失总量与产沙产流之间有着极显著的正相关性,裸地养分流失量最大,其次是谷子地,草地的养分流失量最小,但是径流中硝态氮含量和泥沙总磷量谷子地流失量最大,裸地次之,草地最小。3种土地利用方式片麻岩坡面磷钾流失主要是泥沙态养分,氮素流失主要以溶解态氮为主或者二者共同作用。     

玉米成熟期黄壤坡耕地径流及其氮素流失特征研究

以顺坡垄作、平作、横坡垄作坡面为研究对象,研究自然降雨条件下,黄壤坡耕地地表径流、壤中流及其氮素流失特征,以期为研究区氮素流失预测和有效防控提供科学依据。结果表明:自然降雨条件下,玉米成熟期平均地表径流量分别为0—20,20—40cm壤中流量的7.96,8.22倍。不同耕作措施间地表径流量和氮素流失量差异显著,地表径流量和氮素流失量均表现为顺坡垄作平作横坡垄作,顺坡垄作坡面地表径流量分别是平作和横坡垄作的1.20,2.07倍,顺坡垄作坡面氮素流失量分别是平作和横坡垄作的1.35,2.06倍。在0—20,20—40cm壤中流中横坡垄作径流量和氮素流失量则明显高于其他耕作措施坡面,在0—20cm壤中流中横坡垄作氮素流失量分别是顺坡垄作和平作的2.45,1.90倍;在20—40cm壤中流中横坡垄作氮素流失量分别是顺坡垄作和平作的2.34,1.79倍。地表径流为氮素流失的主要途径,可溶态氮为氮素流失的主要形式,占总氮流失量的63.84%～72.61%;硝态氮是坡耕地无机氮流失的主要成分,占总氮流失量的16.47%～59.17%。氮素流失量与径流量和降雨量均呈显著线性正相关关系。研究区自然降雨条件下,横坡垄作能有效减少氮素流失,合理的耕作措施有助于防治研究区水土资源和氮素流失。     

横坡耕作与优化施肥对缓坡地氮磷流失特征和土壤肥力的影响

[目的] 分析在优化施肥和采取横坡耕作条件下,缓坡耕地的氮磷流失特征和土壤肥力变化,为减少氮磷流失,控制面源防治提供依据,为保护耕地资源、保持土壤肥力质量提供参考。[方法] 以云南省山地丘陵区缓坡地为研究对象,在自然降雨条件下,采用建立地表径流监测小区的方法,设置顺坡耕作+常规施肥;横坡耕作+常规施肥;横坡耕作+优化施肥3种处理,对2019年1月至2021年12月不同耕作与施肥方式下氮、磷流失特征进行了连续监测,采用灰色关联法分析土壤肥力质量。[结果] 氮、磷流失量与降雨量、径流量间存在极显著(p＜0.01)线性相关关系,横坡耕作相比于顺坡耕作可以减少20.46%～40.74%的地表径流,19.12%～33.71%的总氮流失量,33.33%～60.08%的总磷流失量;优化施肥相比于常规施肥可以减少1.20%～51.71%的总氮流失量;各土壤肥力因子除硝态氮和有机质含量减少外,其他均表现为不同程度的提高;各处理下的灰色关联度均表现为不同程度的提高,横坡耕作、优化施肥处理下灰色关联度提高显著(p＜0.05),提高幅度达26.63%。[结论] 横坡耕作对于拦截地表径流和减少氮、磷流失具有良好的效果。采用横坡耕作+优化施肥处理对于提高土壤肥力水平以及减少氮、磷流失量效果最优。     

耕作措施对玉米生长期黄壤坡耕地径流及可溶性有机碳流失的影响

为探明黄壤坡耕地不同耕作措施下径流及可溶性有机碳(dissolved organic carbon,DOC)迁移途径与流失特征。以顺坡垄作、平作和横坡垄作措施下玉米种植坡耕地为研究对象,采用野外径流小区监测与室内分析相结合的方式,探讨自然降雨条件下坡耕地地表径流、壤中流及其DOC流失特征。结果表明:(1)种植玉米坡耕地径流量及DOC通量均受雨量等级影响显著,随雨量等级增大而增大,且玉米成熟期流失量均最大。(2)地表径流、0-20 cm壤中流和20-40 cm壤中流分别占总径流量的78.98%,9.05%,11.97%,DOC流失通量分别占总通量的74.90%,11.97%,13.13%。(3)横坡垄作DOC流失量显著小于顺坡垄作,横坡垄作径流量分别是平作的0.93倍,是顺坡垄作的0.86倍;横坡垄作DOC通量是平作的0.85倍,是顺坡垄作的0.79倍。雨量等级增加将显著加剧玉米种植坡耕地径流及DOC流失,研究区玉米成熟期径流及DOC流失最为严重,地表径流是坡面径流及DOC流失的主要途径,横坡垄作措施可以有效减少坡面径流及DOC流失。     

不同耕作模式下坡耕地次降雨径流量及径流泥沙颗粒机械组成规律

为确定坡耕地在不同耕作模式下,次降雨坡面径流量及径流泥沙中土壤颗粒机械组成规律,在天然降雨条件下,采用10°坡耕地,顺垄、横垄、免耕、秸秆覆盖耕作模式组合试验。结果表明:不同耕作模式试验小区的次降雨径流量顺序为裸地(LD)>顺垄传统耕作(Ts)>顺垄免耕(NTs)>顺垄免耕秸秆覆盖(NTSs)>横垄传统耕作(Th)>横垄免耕(NTh)>横垄免耕秸秆覆盖(NTSh);不同耕作模式下,坡面流失的泥沙颗粒机械组成均不同,其中LD流失的沙粒含量最多,Ts流失的粉粒含量最少,NTSh流失的粘粒含量最多;不同耕作模式,坡面流失土壤颗粒级配较均匀地为LD、NTs、Th、NTh;不同耕作模式产生的地表径流中,粒径在0.25～0.06mm的土壤流失量最多,当粒组的范围>0.06mm时,各粒组不同处理方式的土壤流失量顺序均为NTSh相似文献   

不同施肥和耕作处理紫色土坡耕地碳、氮、磷流失特征

  【目的】  坡耕地营养元素流失是下游水体环境污染的主要原因之一。探明不同施肥处理和耕作方式下紫色土坡耕地碳(C)、氮(N)、磷(P)营养元素流失特征,对采取科学的田间管理措施,降低水生生态系统污染风险具有重要意义。  【方法】  以该区典型坡耕地为研究对象,设置5个施肥和耕作处理:顺坡耕作对照(CK)、顺坡耕作施化肥+猪粪(T1)、顺坡耕作单施化肥(T2)、顺坡耕作单施1.5倍化肥(T3)和横坡耕作单施化肥(T4)。在玉米苗期、拔节期、抽穗期、成熟期,监测了径流量,测定了土壤和径流中的C、N和P含量,计算流失量。  【结果】  与对照相比,T1和T4处理显著增加土壤中有机碳、全氮、全磷含量(P < 0.05)。顺坡耕作T1处理径流中的可溶性有机碳浓度较T2处理降低了33.66% (P < 0.05),其他指标浓度的降低幅度未达显著水平;T3处理的径流中碳、氮、可溶性总磷的浓度较T2 处理有所升高,其中可溶性有机碳和NH₄+-N的提高效果达到显著水平(P < 0.05);T4处理中仅径流NO₃?-N浓度显著低于T2处理(P < 0.05),其他指标与T2处理差异不显著。5个处理的径流量以CK最高,T4处理最低,单施化肥的T2和T3处理均大于化肥猪粪配合的处理T1。横坡耕作的T4处理径流量较其他处理降低了44.65%～67.36%,化肥+猪粪T1处理的径流量较T2、T3处理分别减少了15.65%、22.76%。与T2处理相比,化肥有机肥配施T1处理下的径流可溶性有机碳、全氮和全磷流失量分别减少43.10%、27.51%和31.26%;横坡耕作T4处理下径流可溶性有机碳、全氮和全磷流失量分别降低59.16%、58.57%和68.42%;提高施化肥量T3处理的径流可溶性有机碳、全氮和全磷流失量最高,分别为6.02、0.86和0.20 kg/hm2。  【结论】  在紫色土坡耕地上,与单施化肥相比,相同耕作方向下化肥有机肥配施降低了径流中可溶性有机碳、全氮和全磷浓度,且可溶性有机碳浓度显著降低,横坡耕作主要是大大减少了径流量,因此,采用横坡耕作和化肥有机肥配施是减少土壤C、N、P流失,降低周围水体污染风险的有效措施。     

不同土地利用类型下水土流失特征及雨强关系分析

坡面是黄土丘陵沟壑区土壤侵蚀的重要源地,以岔口小流域4个不同类型的径流场观测数据为基础,对不同土地利用类型下的径流、泥沙和氮流失特征进行研究,并分析不同时段雨强对其的影响。结果表明,裸地的产流和产沙量最大,天然林地产水、产沙及氮流失量最小;年内单位面积径流量与最大30min雨强呈较好的指数函数关系,且拟合程度高于最大20min雨强和最大10min雨强,而与平均雨强呈3次函数关系;单位面积泥沙流失量也表现出相似趋势。坡耕地径流中氮流失浓度最高,尤其是6,7月份的高强度降雨产生的径流,年度内同等程度降雨条件下径流中氮流失浓度呈现前高后低的趋势。     

Phytotoxicity of cobalt,vanadium, titanium,silver, and chromium

Abstract

The phytotoxicity of five nonessential elements (Co, V, Ti, Ag, Cr) to higher plants was studied in solution culture experiments with bush beans (Phaseolus vulgaris L. C.V. Improved Tendergreen). All, but in varying degrees, tended to concentrate in roots with a decreasing gradient to stems and leaves. Cobalt was one of the more mobile of the five trace metals. Its toxicity was expressed as severe chlorosis; 43 (with 10^‐5 M) and 142 (with 10^‐4 M) μg Co/g dry weight in leaves resulted in severe chlorosis. Vanadium as 10^‐4 M vanadate resulted in smaller plants but not in chlorosis. Leaf, stem, and root V, respectively, were 13, 8, and 881 μg/g dry weight. Titanium was somewhat mobile with considerable yield decrease at 10^‐4 M; leaf, stem, and root Ti concentrations, respectively, were 202, 48, and 2420 μg/g. Symptoms were chlorosis, necrotic spots on leaves, and stunting. Silver was very lethal at 10^‐4 M AgNO₃; at 10^‐5 M yields were greatly decreased, but plants were grown without symptoms. Leaf, stem, and root concentrations of Ag for this treatment, respectively, were 5.8, 5.1, and 1760 μg/g dry weight. Plants grown with 10^‐5 N Cr₂O₇ were decreased in yield by about 25% with or without EDTA (ethylenediamine tetraacetic acid) while the same level of Cr₂(SO₄)₃ was essentially without effect. For the two salts, the leaf, stem, root concentrations for Cr, respectively were 2.2 and 1.3, 0.7 and 0. 7, and 140 and 104 μg/g. Most of the trace metals studied here had interactions in the uptake and/or distribution of other elements.     

Bioavailability of As,Cd, Co,Cr, Cu,Hg, Mo,Ni, Pb,Se, and Zn from Biosolids Amended Compost

Appropriate compost standards are being considered in Canada. Five aspects of compost safety and quality are being evaluated; probably the most controversial aspect is the standards for metals in compost. In order to assist in the development of appropriate standards, the authors began an extensive research project in October, 1993 to determine the bioavailability of metals from compost and compost-metal mixtures. Swiss chard was grown in compost-amended soils or compost in a growth room using five treatments of increasing percentages of compost in the media (0, 25 percent, 50 percent, 75 percent, 100 percent compost (v/v)). A Truro loamy sand and a race-track manure-biosolids compost (RTM-biosolids) supplemented with a high metal biosolids were used in a completely randomized design with five replicates. Dry matter yield, metal content in plant tissue, and total metal uptake were evaluated as well as the total and DTPA-extractable metal content in the compost-soil mixes. The results of this and five other experiments conducted by the authors will help determine whether the suggested limits for As, Cd, Co, Cr, Cu, Hg, Mo, Ni, Pb, Se and Zn in composts are appropriate.     

Variation of N,P, K,Ca, Mg,Mn, Zn,and al in slash pine foliage

Abstract

The seasonal patterns of foliage nutrient concentrations and contents were monitored for two growing seasons in an 11‐year—old Pinus el1iottii stand. In the first growing season after needle initiation, N, P, K, Mg, and Zn concentrations decreased, but this was followed by an increase in the fall and winter months. Another drop in concentration of all elements, except P, occurred in the second growing season. Decreases in total contents indicated that this drop was a result of translocation to other tissues. In contrast to the mobile elements, the concentration and fascicle contents of Ca, Mn, and Al increased with aging of the needles.

Between‐tree variability was least for N, P, and Zn and the N, K, Mg, Mn, and Zn in the current foliage had consistently lower variation than that in the 1‐year‐old foliage. Between‐tree variation for K was lower in the winter than the spring.

For pine foliage, recommended sampling period for N, P, Mg, and Zn is mid to late summer and for the other elements it is late fall to late winter.

There are several sources of variation that influence the level of nutrients in tree foliage. The most important of these, apart from the tree nutrient status, are seasonal fluctuations, variation between trees, and age of needles . Smaller sources of variation are associated with position of the needles within the crown, diurnal changes, year to year variation, and analytical errors^1,2. These variables must be studied in order to develop suitable sampling techniques and in Pinus this has been undertaken for P. banksiana ¹, P. taeda ³, P. strobus ⁴, P. resinosa ⁴, P. sylvestris ⁵, and P. radiata ^6,7. However, foliage sampling has not been studied in detail for slash pine (Pinus elliottii Englem var. elliottii) and earlier studies with other pines have been largely confined to temperate or cool climates.

This study reports the variation in elemental concentrations with season, age of foliage, and between slash pine trees growing in a subtropical climate in Florida.     

Thin layer chromatographic determination of aflatoxins, ochratoxins, sterigmatocystin, zearalenone, citrinin, T-2 toxin, diacetoxyscirpenol, penicillic acid, patulin, and penitrem A.

A general method is described for determining 16 mycotoxins in mixed feeds and other food products used in the manufacture of these feedstuffs. The mycotoxins are extracted and cleaned up by extracting with solvents of different pH. Thin layer chromatography is used to separate the toxins; toxins are then quantitated by the limit detection method. The minimum detectable concentration of mycotoxins in various products is: aflatoxin B1 or G1, 4--5 micrograms/kg; ochratoxin A or ethyl ester A 140--145 micrograms/kg; citrinin 600--750 micrograms/kg; zearalenone, 410--500 micrograms/kg; sterigmatocystin, 140--145 micrograms/kg; diacetoxyscirpenol, 2400--2600 micrograms/kg; T-2 toxin, 800--950 micrograms/kg; patulin, 750--800 micrograms/kg; penitrem A 14,000--14,500 micrograms/kg; penicillic acid 3400--3650 micrograms/kg.     

Estimation of Reference Values for Cadmium,Cobalt, Chromium,Copper, Nickel,Lead, and Zinc in Brazilian Soils

Abstract

The objectives of this study were 1) to recommend reference values (RVs) and tolerance limits (TLs) for representative Brazilian soils and 2) to propose a model to calculate natural contents of cadmium (Cd), cobalt (Co), chromium (Cr), copper (Cu), nickel (Ni), lead (Pb), and zinc (Zn) in a soil from the silt, clay, manganese (Mn), iron (Fe), and cation exchange capacity (CEC) values. A set of 256 soil samples was classified by similarity in seven groups, and the concentrations corresponding to the upper quarter of data collected were then calculated. These concentrations are proposed as RVs for Brazilian soils. Additionally, TLs were obtained for each group from the antilog expression (m+2s), where m=mean value and s=standard deviation of data transformed in log₁₀. The classification functions of discriminant analysis proved to be suitable to allocate new samples in the established groups. Thus, it is possible to evaluate soils under anthropic activity and, by comparison with reference values, to be aware of pollution risks in a given area.     

Glucoraphanin and 4-hydroxyglucobrassicin contents in seeds of 59 cultivars of broccoli, raab, kohlrabi, radish, cauliflower, brussels sprouts, kale, and cabbage

The importance of dietary sulforaphane in helping maintain good health continues to gain support within the health-care community and awareness among U.S. consumers. In addition to the traditional avenue for obtaining sulforaphane, namely, the consumption of appropriate cruciferous vegetables, other consumer products containing added glucoraphanin, the natural precursor to sulforaphane, are now appearing in the United States. Crucifer seeds are a likely source for obtaining glucoraphanin, owing to a higher concentration of glucoraphanin and the relative ease of processing seeds as compared to vegetative parts. Seeds of several commonly consumed crucifers were analyzed not only for glucoraphanin but also for components that might have negative health implications, such as certain indole-containing glucosinolates and erucic acid-containing lipids. Glucoraphanin, 4-hydroxyglucobrassicin, other glucosinolates, and lipid erucic acid were quantified in seeds of 33 commercially available cultivars of broccoli, 4 cultivars each of kohlrabi, radish, cauliflower, Brussels sprouts, kale, and cabbage, and 2 cultivars of raab.     

Distribution of Zinc,Manganese, Copper,Cobalt, and Nickel in Andosols Profiles

The distribution of zinc, manganese, copper, cobalt, and nickel in Andosols was investigated. Sixty nine soil samples were collected from different horizons of an Andosols profile in Miyakonojo Basin in south Kyushu, Japan, The total contents of heavy metals were determined by digestion and four extraction solutions, 1 M NH₄Ac (ammonium acetate) pH 4.5, 0.1 M HCl, 0.01 M EDTA (ethylenediaminetetraacetic acid) pH 6.5, and 0.005 M DTPA (diethylenetri-aminepentaacetic acid) pH 7.3 were used to determine the contents of available Zn, Mn, Cu, Co, and Ni in Andosols in relation to the organic carbon content. The results of the extraction analysis showed that by the use of 0.1 M H Cl high value of extracted heavy metals in the upper layers of the humus horizons were obtained while EDTA extraction yielded a large amount of the above mentioned metals in the high humus horizons. The extractable heavy metals contents were high and these metals closely related to the organic carbon content mostly in the humus horizons in the profile. Where, biocycling process may play an important role in the concentration of heavy metals. Based on the study, it was found that the total content of Zn increased towards the C horizons or pumice layers in the soil profile. Such a trend was also found in the case of the Mn content. While the Cu content in the humus horizons was much higher in the upper part of each humus horizon. According to this study the distribution of heavy metals, Cu (organic matter complexes) in the Andosols profile was more stable than that of Zn (organic matter complexes) in soils. It was shown that Zn in the surface humus horizon was enriched but that some amount was leached under buried conditions. The same phenomenon was also observed in the distribution of Mn in the profile. The movement of Co and Ni in the soil profile was limited, as evidenced by the sharp reduction in the concentrations of these two metals in buried soils.

Hence, it is concluded that the distribution of Zn, Mn, Cu, Co, and Ni was considerably higher in the humus horizons of the Andosols profiles.     

Cooking,Roasting, and Fermentation of Chickpeas,Lentils, Peas,and Soybeans for Fortification of Leavened Bread

The effects of cooking, roasting, and fermentation on the composition and protein properties of grain legumes and the characteristics of dough and bread incorporated with legume flours were determined to identify an appropriate pretreatment. Oligosaccharide content of legumes was reduced by 76.2–96.9% by fermentation, 44.0–64.0% by roasting, and 28.4–70.1% by cooking. Cooking and roasting decreased protein solubility but improved in vitro protein digestibility. Mixograph absorption of wheat and legume flour blends increased from 50–52% for raw legumes to 68–76, 62–64, and 74–80% for cooked, roasted, and fermented ones, respectively. Bread dough with cooked or roasted legume flour was less sticky than that with raw or fermented legume flour. Loaf volume of bread baked from wheat and raw or roasted legume flour blends with or without gluten addition was consistently highest for chickpeas, less for peas and lentils, and lowest for soybeans. Roasted legume flour exhibited more appealing aroma and greater loaf volume of bread than cooked legume flour, and it appears to be the most appropriate preprocessing method for incorporation into bread.     

Hydrophilic chromatographic determination of carnosine, anserine, balenine, creatine, and creatinine

A new HPLC procedure based on hydrophilic interaction chromatography (HILIC) has been developed for the simultaneous determination of carnosine, anserine, balenine, creatine, and creatinine in meat. This is the first time that HILIC has been directly applied to the study of meat components, having the advantage of not requiring complex cleanup and/or sample derivatization procedures. The chromatographic separation has been developed using a silica column (4.6 x 150 mm, 3 microm), and the proposed methodology is simple, reliable, and fast (<13 min per sample). The method has been validated in terms of linearity, repeatability, reproducibility, and recovery and represents an interesting alternative to methods currently in use for determining the mentioned compounds and other polar substances. The detection limits are 5.64, 8.23, 3.66, 3.99, and 0.06 microg/mL for carnosine, anserine, balenine, creatine, and creatinine, respectively.     

Volatilization of trifluralin,atrazine, metolachlor,chlorpyrifos, alpha-endosulfan,and beta-endosulfan from freshly tilled soil

The volatile and soil loss profiles of six agricultural pesticides were measured for 20 days following treatment to freshly tilled soil at the Beltsville Agricultural Research Center. The volatile fluxes were determined using the Theoretical Profile Shape (TPS) method. Polyurethane foam plugs were used to collect the gas-phase levels of the pesticides at the TPS-defined critical height above a treated field. Surface-soil (0-8 cm) samples were collected on each day of air sampling. The order of the volatile flux losses was trifluralin > alpha-endosulfan > chlorpyrifos > metolachlor > atrazine > beta-endosulfan. The magnitude of the losses ranged from 14.1% of nominal applied amounts of trifluralin to 2.5% of beta-endosulfan. The daily loss profiles were typical of those observed by others for volatile flux of pesticides from moist soil. Even though heavy rains occurred from the first to third day after treatment, the majority of the losses took place within 4 days of treatment, that is, 59% of the total applied atrazine and metolachlor and >78% of the other pesticides. Soil losses generally followed pseudo-first-order kinetics; however, leaching due to heavy rainfall caused significant errors in these results. The portion of soil losses that were accounted for by the volatile fluxes was ordered as follows: alpha-endosulfan, 34.5%; trifluralin, 26.5%; chlorpyrifos, 23.3%; beta-endosulfan, 14.5%; metolachlor, 12.4%; and atrazine, 7.5%.