Aluminium Toxicity Risk for Pinus pinaster in Acid Soils (Galicia,NW Spain)

The influence of bedrock on aluminium toxicity and aluminium speciation in the soil solution was studied in four Pinus pinaster plots. Growth and biomass parameters in the acidic soils were also evaluated in relation to different Al toxicity indices. The plots were developed over slate, biotitic schist, mica schist and granite. Samples of rhizospheric and non‐rhizospheric soil, 1‐year‐old needles and roots were collected in each study plot. Total Al, reactive Al, acid‐soluble Al, non‐labile and labile Al and Al species (Al³⁺, Al‐OH, Al‐F and Al‐SO₄) were determined in soil solution. Reactive Al dominated over the acid‐soluble Al, and the non‐labile Al predominated over the labile Al in all soils, but particularly over mica schist. In the biotitic schist soil, the Al forms and total Al were lower, whereas concentrations were always higher over mica schist. The Al forms considered most toxic were Al³⁺ and Al‐OH, and Al concentrations were highest over slate and mica schist. Al toxicity indices in soil, needle and roots showed a risk of toxicity in mica schist, slate and granite. The stand site indices over slate and mica schist were lower, consistent with the high labile Al and Al³⁺ + Al‐OH in soil solution. Despite the high stand site index over granite, the growth efficiency was low, in accordance with very low ratios of Ca/Al in needles or fine roots. This confirmed the adaptation of maritime pine to granitic substrates. Copyright © 2016 John Wiley & Sons, Ltd.     

Soil Properties and Selected Aluminium Forms in Acid Forest Soils as Influenced by the Type of Stand Factors

Massive forest damage has been observed in certain mountainous regions affected by acidification. Declining of trees is ascribed to direct effect of SO₂ and also increasing concentration of mobile forms of Al in soils. The aim of this paper is to describe the influence of stand factors to distribution of Al forms in the Jizera Mountains region, Czech Republic. A set of 251 samples from 98 sites was collected. Basic soil characteristics and the content of different Al forms were determined. The amount and relative share of these Al forms under different types of vegetation was evaluated, with emphasis on the surface organic horizons. The results showed that phytotoxic mobile Al forms are the most influenced by altitude and organically bound Al forms by forest type. Non-toxic forms of Al are more abundant in broadleaved forest. Soils under coniferous forest are more affected by acidification.     

Abstracts of nippon Dojohiryogaku Zasshi

The effects of air temperature and light on the grain filling of an indica (IR20) and ajaponica rice (Fujisaka 5) was studied in artificially lighted cabinets. Within the daily mean temperature range of 16° to 28°C, the higher the temperature, the faster the grains filled and matured. At 28°C, the upper grains of IR20 rice took 13 days to reach the maximum weight, whereas those of Fujisaka 5 took 18 days. The optimum daily mean temperature range to achieve maximum weight per grain was 19° to 25°C for IR20 and 16° to 22°C for Fujisaka 5.

Apparently, IR20 rice is better adapted to higher temperatures during the ripening period than is Fujisaka 5 rice. More chalky grains occurred when the temperature was above or below the optimum range. Both day and night temperatures affected grain weight and grain quality.

The daily mean temperature was found to be the most meaningful expression for describing the effect of temperature on grain filling. Low light intensity appeared to cause a slight delay in the grain filling of the whole panicle and a reduction in the percentage of filled grains on the lower branches. A combination of high light intensity and low temperature gave the best ripening grade (grain weight × percent filled grains).     

Aluminium and lead tolerance in ectomycorrhizal fungi

Al and Pb tolerance of different ectomycorrhizal fungi was tested in Petri dishes divided into three compartments. One compartment contained nutrient solution supplemented with Al or Pb but without P to avoid precipitation of Al or Pb phosphates. Phosphate was supplied in the agar compartment while a third compartment served as control containing nutrient solution without P. The tested ectomycorrhizal fungal species and isolates varied greatly in their Al and Pb tolerance. A more than 50% growth reduction was observed at 2000 μM Al in Paxillus involutus MAI but already at 200 μM in P. involutus NAU and P. involutus 533. In contrast, growth of Pisolithus tinctorius 956 appeared to be stimulated by Al. Al tolerance therefore decreased in the following order: P. tinctorius 956 > P. involutus MAI > P. involutus NAU, P. involutus 533. To test the effect of agar on Al tolerance, P. involutus MAI was grown with agar in all three compartments. In presence of agar, even 2000 μM Al had no effect on growth. Lead at concentrations of 1 and 10 μM Pb had no significant effect on fungal growth. A more than 50% decrease of mycelial surface area occurred at 500 μM Pb in P. involutus MAI and P. involutus 533 and at 100 μM Pb in P. involutus NAU and P. tinctorius 956. Pb tolerance therefore decreased in the following order: P. involutus MAI, P. involutus 533 > P. involutus NAU, P. tinctorius 956. Divided Petri plates appear to be a valuable tool to test metal tolerance of fungi as complexation of the metals by agar or precipitation with P are avoided. The results indicate that some ectomycorrhizal fungi may be more sensitive to Al and Pb than previously reported.     

氟化物对酸性土壤溶液组成的影响

Atmospheric emissions of fluoride from an aluminium smelter-alumina refinery located on the northern coast of Galicia, NW Spain, increase the content of fluorine in soils and vegetation in the vicinity of the complex. The effects of the addition of fluoride solutions on the chemical properties of soil samples from the area surrounding the complex were investigated in laboratory experiments. Addition of fluoride to soils resulted in increases in pH and concentrations of Fe, Al, and organic matter in the equilibrium solutions and decreases in concentrations of Ca, Mg, and K. No consistent effects were observed on the Cu, Mn, or Zn concentrations. Most of the Al in solution was bound to organic matter. Within the fraction “labile aluminium,” the concentration of Al-OH complexes decreased and the Al-F complexes increased, especially AlF3 and AlF4-, which are less toxic than Al3+ and Al-OH species.     

Sand culture of mycorrhizal plants

In this work, a sand culture system for ecto- or arbuscular mycorrhizal plants was developed. Nutrients were added into the sand at a constant rate using solutions with similar concentrations of nutrients as those found in forest soil solutions. Plants grew well in the system and inoculated plants developed abundant ecto- or arbuscular mycorrhizas. To test the suitability of the culture system for studies on metal toxicity, aluminium was added to the nutrient solutions of non-mycorrhizal and mycorrhizal spruce seedlings. Measurments of labile Al in the solution draining from the sand revealed that Al was mainly present as phytotoxic monomeric Al. In addition, concentrations of Ca²⁺, Mg²⁺ and SO₄^2— ions which have the potential to alleviate Al toxicity, were similar in the draining as in the nutrient solutions. After 10 weeks of exposure to 400 μM Al, taproot growth as well as Ca and Mg uptake of the seedlings were impaired by Al. Mycorrhizal colonization had no effect on the degree of inhibition of these processes. We conclude that this culture system is suitable for investigations on effects of Al and other toxic metals on mycorrhizal tree seedlings.     

腐殖酸对磷在红壤中有效性的影响

An investigation was conducted to study the effect of humic substance （HS） on the phosphorus （P） solubility in acidic soil. The soil （2.5 g）, HS （0, 0.5, and 2.5 g）, and P as monocalcium phosphate （0.31 and 1.25 g P kg^-1 soil） were mixed with 50 mL distilled water and two different sequences of adding HS and P were used. The results indicated that the P concentration in water and 0.01 mol L-1 CaCl2 solution increased with increasing amounts of humic substance. The concentrations of Fe and Al were also increased. However, Olsen P decreased with increasing amount of humic substance. Water-soluble P concentrations from P rates at 0.31 and 1.25 g P kg^-1 soil in the treatment with 0.5 g （2.5 g） humic substance addition were 360% and 70% （500% and 90%） higher, respectively, than those in the treatment with no humic substance addition. P extracted by 0.01 mol L^-1 CaCl2 in the treatments with 0.5 and 2.5 g humic substance addition was increased by 400% and 540%, respectively, compared with that in the treatment without humic substance at the rate of 0.31 g P kg^-1 soil, while the corresponding P concentrations were increased by 80% and 90% at the rate of 1.25 g P kg^-1 soil. The order of mixing humic substance and phosphate did not significantly affect desorbed P and labile P extracted with CaCl2.     

Some Approaches to the Research of Forest Soils Affected by Acidification in the Czech Republic

Soil acidification in mountainous regions of the Czech Republic presents a serious problem. This paper summarizes several projects dealing with this problem exploiting different methods and having different objectives: 1) Long-term soil and forest development in the Krkonoše Mountains. 2) Soil and forest development along an elevation transect in the Šumava Mountains. 3) Long-term effects of liming. 4) Comparison of soil acidification between three mountainous regions, with emphasis on labile Al content. 5) Spatial distribution and factors of soil acidification and Al release in the Jizera Mountains. The results of these projects showed that acidification in forest soils in the mountainous areas is caused by a combination of different factors, especially by the type of vegetation, atmospheric deposition, parent rock, altitude, and others. A slight improvement of soil properties is apparent after the decrease of immissions in the 1990's.     

Extraordinary high aluminium tolerance of the acidophilic thermophilic alga,Cyanidium caldarium

A strain of an acidophilic, thermophilic alga, Cyanidium caldarium, was cultured in a medium containing various metal ions (A1, Cd, Cr, Cu, Mn, Ni, Zn). Among these metals, the alga tolerates especially high levels of AI: it can grow in a medium containing 200 mM AI, although the growth rate was reduced to 58%. The cellular Al concentration was kept at a considerably lower level as compared to the medium Al concentration. This may account for the Al tolerance of the alga. Treatment of the algal cells with carbonylcyanide m-chlorophenylhydrazone in the presence of Al increased the cellular Al concentration. It was suggested that energy-coupled Al exclusion mechanisms can operate in the alga.     

Application of P and Al MAS NMR for phosphate speciation studies in soil and aluminium hydroxides: promises and constraints

Magic angle spinning (MAS) nuclear magnetic resonance (NMR) was used to investigate the chemical environment of P in soil and soil components. ³¹P and ²⁷Al MAS NMR spectra are presented of synthetic aluminium hydroxides (amorphous aluminium hydroxide and gibbsite), reacted with P under different conditions of P concentration, temperature and pH. The reaction product is amorphous octahedral aluminium phosphate, which transforms (partly reversibly) to tetrahedral aluminium phosphate upon drying. Results of several experiments on excessively fertilized sandy soil material are discussed. The soil particle fraction smaller than 50 μm was used for NMR analysis. ³¹P and ²⁷Al MAS NMR spectra show a Ca---P pool and an Al---P pool. A six-fold water extraction removes part of both P pools. Oxalate extraction removes all Ca---P and Al---P from the sample. Removal of the labile P pool by the HFO-DMT long-term P desorption technique, does not drastically change the ³¹P MAS NMR spectrum. The formerly mentioned Ca---P and Al---P are thus stable P pools. The ¹H---³¹P cross-polarization (CP) spectrum of the original soil sample revealed a third chemical environment, which was identified as labile Ca---P: this P pool does not appear in the ¹H---³¹P CP spectrum of the soil sample from which the fast P pool had been removed. The combination of MAS and CP MAS NMR can thus reveal at least three different P species in soil, of which two pools were identified as stable, and one as labile.     

The Contribution of Various Components to pH Buffering Capacity of Acrisols in Southeastern Vietnam

This paper examined the contribution of various soil components to pH buffering capacity (pH_BC) of haplic Acrisols in an upland area of Southeastern Vietnam. Sampling was conducted in 2016 in Tan Bien district, Tay Ninh province at seven sites from the surface to 60-cm depth. Soils were very acidic (pH_H2O 4.53 ± 0.05). The pH_BC were very low, 0.46 ± 0.04 and 0.44 ± 0.05 cmol H⁺ kg^?1 pH^?1, respectively, for original samples and those from which soil organic carbon (SOC, 0.52 ± 0.09%) were removed. The contribution of Al³⁺ to pH_BC was remarkable while that of SOC was of little significance. The contribution of clay minerals to pH_BC was unclear due to the low (9.37 ± 0.76%) and kaolinite-dominated clay content. The current soil conditions indicated a potential for further soil acidification. Liming would be one of the measures to remediate soil acidity in the research area.     

Extraction methods for aluminium and iron in relation to phosphate adsorption

Abstract

Three extraction methods for aluminium and two for iron were compared with phosphate sorptivity (Bache and Williams index) for 40 tropical and 40 British soil samples. Extractable aluminium was well correlated with phosphate sorptivity in both groups, but extractable iron was well correlated only in British soils. In general methods which extracted more sesquioxides gave higher correlation coefficients. With aluminium, N ammonium acetate (pH 4.8) or 0.1N HCl gave better correlation than N KCl, while with iron dithionitecitrate was better than 0.1N HCl.     

Cations adsorbed to soil organic matter — A regulatory factor for the release of organic carbon and hydrogen ions from soils to waters

Surface waters in northern forest ecosystems receive a substantial amount of drainage water from superficial soil horizons enriched in organic matter (SOM). Chemical reactions in the interface between the soil solution andf organic colloides will therefore affect the surface water chemistry. The mobilization of total organic carbon (TOC) and pH was studied as a function of amounts of organically adsorbed Na, Ca and Al in two O and one A horizon, which differed in the likelihood of contributing to the chemistry in runoff, in a forested watershed in northern Sweden. The samples were hydrogen ion saturated, washed and titrated with NaOH, Ca(OH)₂ and Al(OH)₃ in a constant ionic medium of 0.01 M NaCl in order to give rise to a population of manipulated samples differing in the composition of adsorbed cations. The highly humified SOM accumulated in the O_h and A_h horizons of a Gleysol close to the draining stream was stabilized by flocculating Al (95% of adsorbed metal cations), which resulted in a low release of TOC. These horizons showed a high potential of organic carbon solubility when Al was changed for di- or monovalent cations. Calculations suggested that the release of TOC would increase more than ten times if Al was exchanged for Ca upon liming to pH 6.0. The pH values of all horizons were shown to be determined mainly by the composition of adsorbed mono-,di- and trivalent cations.     

磷石膏改良强酸性黄壤的效应研究

采用盆栽试验,以耐铝能力弱的高粱和强酸性黄壤为研究对象,探讨了磷石膏对强酸性环境高粱生长、养分平衡以及对土壤有效养分和交换铝的影响。结果表明,在强酸性黄壤上,由于铝毒作用,即使施足氮磷钾肥,高粱也生长不良;施用磷石膏和石灰的高粱生长正常,各生物学性状均极显著优于对照,施磷石膏高粱长势更优于施石灰者,高粱干重与磷石膏、石灰的施用量呈二次曲线变化(回归方程分别为:y=6.88 11.92x-1.65x2,R=0.983;y=6.88 6.39x-0.72x2,R=0.996),高粱株高和根长与磷石膏、石灰的施用量也呈二次曲线变化;磷石膏降低酸性黄壤铝毒、提高植物钙含量的效果略逊于石灰,在提高植物营养三要素(特别是磷、钾元素)的作用方面优于石灰,施磷石膏高粱磷、钾含量较石灰处理分别提高117.0%~200.0%和13.0%~14.8%;施磷石膏生长良好的高粱植株氮/磷、氮/钾、钾/磷比分别为6.8~7.1,1.2~1.3和5.4~5.6,比值适中、变幅小,氮/铝、磷/铝、钾/铝、钙/铝比分别是对照植株的1.9~2.0倍,5.8~6.3倍,1.8~2.1倍和2.0~2.5倍,改善和调节了高粱体内氮、磷、钾、钙养分的平衡;施磷石膏的土壤有效氮、磷、钾、钙较对照极显著增加,土壤有效磷、钾分别比施石灰提高41.8%~114.2%和59.4%~67.5%,施石灰的土壤有效磷、钾与对照差异不显著。     

磷对植物铝毒害作用研究中两种磷铝处理方法比较

由于磷和铝在溶液中的相互作用,研究磷对植物铝毒害作用的关键是选择合适的磷铝处理方法,本研究采用两种磷铝处理方法就磷对植物铝毒害的作用进行了比较。结果表明,采用计算铝活度的方法,磷能缓解铝对小麦的毒害,而采用磷铝交替处理的方法,磷则加重铝对小麦的毒害。尽管在计算铝活度的方法中不同磷浓度处理液中铝的活度相同(用GEOCHEM软件计算),但磷浓度越高的处理液,其毒性铝的浓度越低,在该方法中,磷可能是通过降低溶液中毒性铝的浓度而缓解铝毒的,不能很好地避免溶液中磷铝相互作用。磷铝交替处理彻底避免了磷铝在溶液中的相互影响,保证相同的磷和铝处理浓度,而且长期交替处理在一定程度上也降低了磷铝不能同时处理对试验结果准确性的影响,因而,该方法是一种研究植物磷铝关系比较可行的方法。在磷铝交替处理条件下,磷加重小麦的铝毒害,可能与磷促进铝在根尖的累积有关。     

不同利用方式棕壤的铝硅氧化物的分异特征

棕壤是我国重要的土壤资源,其特殊的气候地形条件决定了必须科学合理的开发利用才能保证该地区农业的可持续发展。本文对林地、蚕地、耕地3种利用方式下棕壤中铝硅氧化物的变化情况进行了研究。结果表明:(1)不同利用方式下,铝氧化物的无定形态在各层间差异较大。0~20 cm土层的游离硅、无定形铝均表现出随人为干预程度的提高而增加的趋势,反映出开垦的集约化加剧了土壤的风化作用。(2)从各形态氧化物在剖面间的分布来看,游离氧化硅、无定形铝的分布均呈向下富集的趋势,与粘粒在土体内的分布规律一致。活性硅与不同利用方式土壤的风化淋溶作用关系的规律性不明显,但可反映作物对硅的吸收特性。(3)开垦后土壤表层生物积累量的减少,影响了土壤表层活性硅的积累。     

Responses to aluminium of suspension-cultured tobacco cells in a simple calcium solution

In a simple solution containing 3 mM CaCl₂ and 3% sucrose (pH 4.5), tobacco cells (Nicotiana tabacum L.) at the logarithmic phase of growth remained viable at least for 24 h. In this medium, the toxic effect of aluminium (Al) on the plasma membrane was investigated for up to 24 h. After the addition of Al to the cell suspension, Al started to accumulate immediately in the cells, and a maximum value was observed at 9 h. Al induced callose deposition, but did not enhance significantly the uptake of Evans blue (a nonpermeating dye), the per oxidation of lipids and the leakage of potassium (K) ions. Furthermore, the AI-treated cells were stained with fluorescein diacetate (FDA) as much as untreated control cells. These results suggest that the accumulation of Al does not damage the membrane. The addition of Fe(II) to the cells which had been exposed to Al for 12 h resulted in immediate lipid peroxidation and Evans blue uptake several hours later. A combination of Al and Fe(II) caused the K leakage, and enhanced the deposition of callose more than Al alone. These results suggest that the accumulation of Al sensitizes the membrane to the Fe(II)-mediated peroxidation of lipids, and that the Al-enhanced per oxidation of lipids is a direct cause of the loss of integrity of the plasma membrane (or cell death) in the Ca medium.     

Aluminium tolerance mechanisms in Phaseolus vulgaris L.: Citrate synthase activity and TTC reduction are well correlated with citrate secretion

We characterised the physiological mechanisms of tolerance in two bean varieties (Phaseolus vulgaris L. viz. French Bean cv. Amy and Rosecoco (GLP2)) differing in Al tolerance of the varietal level. Root elongation at varying levels of Al over time clearly showed the Al tolerance superiority of Rosecoco over French bean. Aluminium uptake was much higher in French bean, in both root apex and 2 mm region of the root apex. The root cation exchange capacity of the Al-sensitive French bean was markedly higher than that of Rosecoco. Citric acid was the only organic acid whose secretion was stimulated by Al and was higher in Rosecoco than in French bean. The citrate synthase and NADP⁺-isocitrate dehydrogenase activities were apparently higher in Rosecoco than in French bean b1,lt those of phosphoenolpyruvate carboxylase were not significantly different between the two varieties under Al stress. Triphenyl-tetrazolium chloride (TTC) reduction was greater in Rosecoco and was also well correlated with the citrate secretion. These results suggest the role of TTC reduction and citrate secretion as underlying factors in the Al tolerance mechanism of Rosecoco.     

Relationships between the Ratio of Plant-Available Phosphorus (P-AL) to Total Phosphorus and Soil Properties

Abstract

The relationships between the ratio of plant-available phosphorus (P-AL) to total P and soil properties were examined in 52 samples of mineral soils collected from different parts of Norway. The ratio P-AL/total P in the soils was used as a measurement of a soil's capacity to bind P in sparsely soluble forms and of the possibility for plants to utilize added P. Simple regression analysis showed that the ratio P-AL/total P was correlated with clay (r= ?0.60???, significant at the 0.1% probability level), Tamm acid oxalate extractable Fe (r= ?0.63???), and Tamm acid oxalate extractable Al (r-= ?0.44???), but not with organic C and pH. Variation of Fe, Al and clay content could explain 50% of the variation of the ratio of P-Al/total P. Partial correlation coefficients showed that Fe was the most important factor explaining the variation of the ratio of P-AL/total P.     

Cell death process initiated by a combination of aluminium and iron in suspension-cultured tobacco cells (Nicotiana tabacum): Apoptosis-like cell death mediated by calcium and proteinase

Aluminium (AI) enhances ferrous ion (Fe²⁺) -mediated lipid per oxidation which is the primary factor leading to cell death in nutrient medium in tobacco cells. Under these conditions, cell death processes were examined. Cells which had been treated with AI and Fe²⁺ together exhibited cell shrinkage and DNA ladders which were detected by agarose-gel electrophoresis in the form of bands corresponding to increasing multiples of ～150 bp fragments. In the calcium (Ca)-free medium, a combination of Al and Fe2+ enhanced only partly the per oxidation of lipids and hardly promoted cell death, indicating that Ca²⁺ further stimulates the per oxidation of lipids enhanced by a combination of Al and Fe²⁺ and is required for cell death. N-Tosyl-L-phenylalanylchloromethyl ketone, an inhibitor of some cysteine and serine proteinases, had no effect on the per oxidation of lipids but almost completely blocked cell death enhanced by a combination of AI and Fe²⁺, suggesting that proteinase acts downstream from the per oxidation of lipids. On the basis of these findings, we conclude that cell death initiated by a combination of Al and Fe²⁺ is involved in the apoptosislike cell death program mediated by extracellular Ca²⁺ and endogenous proteinase.