不同施氮量对林地和农田黑土净氮转化速率的影响

用在土壤含水量为田间持水量60%、温度20℃恒定条件下进行室内培养的方法,研究不同NH_4~+-N施用量(0、40、60、80 mg kg~(-1))对林地和农田黑土净氮转化速率的影响。结果表明,在不施氮的条件下林地黑土的净氮矿化速率为负值,表现为对氮的净固定;在施氮条件下林地黑土的净氮矿化速率为正值,表现为对氮的净矿化,且随NH_4~+-N施用量(0～60 mg kg~(-1))的增加而增加,但NH_4~+-N施用量超过60 mg kg~(-1)后土壤净氮矿化速率反而稍有降低。施氮对农田土壤氮矿化的影响与林地土壤相反,不施氮的农田土壤净氮矿化速率为正值,施氮后土壤净氮矿化速率为负值,且随NH_4~+-N施用量(0～80 mg kg~(-1))的增加而降低。施用NH_4~+-N肥显著促进了林地和农田土壤硝化作用,但对农田土壤的促进程度更大。对于林地土壤而言,施氮处理土壤的净硝化速率是不施氮处理的1.52～1.70倍,不同施氮量处理间差异不显著。对于农田土壤而言,施氮处理土壤的净硝化速率是不施氮处理的16.7～26.4倍,且随施氮量的增加而显著增加。施氮降低了林地土壤的硝化率,但是对农田土壤的硝化率没有显著影响。     

农田黑土氮素转化特征对冻融作用的响应

为了深入了解非生长季农田黑土氮素转化过程,采用室内冻融模拟培养试验研究了不同冻融因子[冻融温度(冻结温度:-3、-6、-9、-12、-15℃;融化温度:2、5℃)、冻融循环次数(1、3、6、10、15;其中在-3℃冻结6 d、2℃融化1 d为1个冻融循环次数)、水分含量(10%、20%、30%)]对农田黑土无机氮组分含量及氮素转化速率的影响。结果表明,较大的冻融温差(-15℃/2～5℃)、适宜的冻融循环次数(1～3)和水分含量(20%～30%)是影响农田黑土氮素转化的主要驱动因子。冻融土壤铵态氮含量、硝态氮含量、净氮矿化速率和硝化速率均随着冻结温度降低显著增加,均随着融化温度升高无显著性变化。随着冻融循环次数增加,冻融土壤铵态氮含量、硝态氮含量、净氮矿化速率和硝化速率均显著降低。随着水分含量增加,冻融土壤铵态氮含量显著增加,这与硝态氮的变化趋势相反,而净氮矿化速率和硝化速率均无显著性变化。可见,冻融作用显著促进非生长季农田黑土氮素转化,有利于土壤有效氮的累积。     

施用猪粪条件下重金属对土壤氮素净转化的影响

以东北耕作黑土为研究对象,在25℃和70%WHC水分条件下进行为期35天的室内培养试验,研究了施用猪粪条件下重金属Cu、Cd对土壤氮素净转化的影响。结果表明:添加单一重金属显著抑制了土壤的硝化作用和矿化作用。与对照处理相比,添加重金属Cu、Cd处理培养结束后土壤铵态氮含量分别增加了5.83和5.39倍,硝态氮含量分别下降了84.3%和79.5%,且土壤净氮矿化速率和净硝化速率均显著低于对照处理。添加重金属的同时施用猪粪加剧了重金属对硝化作用和矿化作用的抑制,土壤净硝化速率和净氮矿化速率均显著低于单一重金属处理。添加重金属抑制了土壤反硝化作用,但同时添加猪粪在一定程度上降低了重金属离子的活性和毒性,进而减轻重金属离子对反硝化作用的抑制程度。     

浅层淹水条件下不同施肥处理对黑土氮素净转化的影响

以东北旱作黑土为对象,在25℃和浅层淹水条件下开展了为期35 d的室内培养试验,研究不同施肥处理对黑土矿化作用和硝化作用的影响。结果表明,浅层淹水条件下土壤矿化作用和硝化作用仍能进行。与不施肥对照处理相比,施用氮肥抑制了培养初期的有机氮矿化,但对后期氮矿化没有影响。氮肥施用初期对硝化作用没有影响,但2周后显著促进了硝化作用的进行。培养期间单施氮肥处理的平均净矿化速率为N 1.07 mg/(kg·d),与对照处理没有显著差异;平均净硝化速率为N 4.50 mg/(kg·d),是对照处理的2.43倍。浅层淹水条件下氮肥配施有机物料显著促进了土壤无机氮的生物固定,培养初期氮肥配施秸秆处理的无机氮固定量大于氮肥配施猪粪处理,后期则相对稍低,氮肥配施猪粪和配施秸秆处理的平均净氮矿化速率分别为N-5.61和-3.15 mg/(kg·d),两者间差异显著。与单施氮肥处理相比,浅层淹水条件下氮肥配施有机物料显著抑制了土壤硝化作用,培养期间氮肥配施猪粪和氮肥配施秸秆处理的平均净硝化速率分别为N 0.29和0.18 mg/(kg·d),分别比单施氮肥处理下降了93.5%和96.0%。     

模拟氮沉降对小兴安岭地区人工红松林土壤氮转化的影响

以我国小兴安岭地区凉水国家级自然保护区30年林龄的人工红松林为研究对象,建立对照(0 kg hm~(-2)a~(-1))、低氮(20 kg hm~(-2)a~(-1))、中氮(40 kg hm~(-2)a~(-1))和高氮(80 kg hm~(-2)a~(-1))四种模拟氮沉降水平的样地;采用室内培养试验,研究了短期模拟氮沉降对人工红松林土壤氮净矿化、净硝化速率和氧化亚氮排放的影响。结果表明,与对照处理相比,经过2年的模拟氮沉降处理,土壤的净矿化、净硝化速率都有降低趋势。与对照相比,低氮、中氮、高氮土壤净矿化速率分别降低了16.9%、20.6%和25.2%,土壤净硝化速率分别降低了16.7%、20.9%和25.5%,但是处理间差异没有达到显著水平。净硝化速率与净矿化速率呈显著正相关关系(P0.05),表明净矿化速率降低减少铵态氮供应量可能是氮沉降处理降低净硝化速率重要原因。另外,模拟氮沉降处理导致土壤p H降低也不利于硝化作用。中氮和高氮处理土壤氧化亚氮累积排放量分别比对照高84%和40%,但是差异不显著。高的氮沉降量使硝化过程中氧化亚氮的排放比例增加,可能是中氮和高氮处理下净硝化速率下降而土壤氧化亚氮排放量却增加主要原因。研究结果表明,氮沉降会影响我国小兴安岭地区森林土壤氮矿化和硝化过程,但是由于实验开展观测时间较短,其影响规律还需长期实验验证。     

祁连山东段青海云杉林区土壤氮矿化与土壤因子的相关性

以祁连山东段青海云杉(Picea crassifolia)林分布带土壤为研究对象,采用顶盖埋管的野外取样法和室内分析法,对海拔梯度上土壤铵态氮(NH+4-N)、硝态氮(NO-3-N)净矿化速率、氮净矿化量和净矿化速率进行测定分析,旨在探讨土壤净氮矿化量与气温降水和土壤理化性质的相关关系,以期建立环境变量与土壤氮矿化量和矿化速率模型,进而提高祁连山青海云杉林生产力及水源涵养能力。其结果表明:(1)土壤硝态氮、铵态氮净矿化速率、土壤净氮矿化量和矿化速率随海拔的升高差异性均极显著;土壤氮净矿化量和矿化速率随海拔梯度的升高呈"W"形变化,与硝态氮净矿化速率随海拔升高的变化规律一致,与铵态氮净矿化速率变化规律相反;在海拔2 800m处,硝态氮净矿化速率、土壤净氮矿化量和矿化速率均达到最大值,为0.372,160.3,0.44 mg/(kg·d),铵态氮净矿化速率出现最低值0.067 mg/(kg·d);在海拔2 900m处出现最低值,为0.155,94.7,0.26mg/(kg·d),在海拔3 100m处,铵态氮的净矿化速率出现最大值0.13mg/(kg·d);(2)回归分析表明,土壤净氮矿化量与年均气温呈极显著负相关(P0.01),R2=0.717 3;与年降水量呈极显著正相关(P0.01),R2=0.383 5;得出气候变化对土壤净氮矿化量的影响程度为:年均气温年降水量;(3)回归分析表明,土壤氮净矿化量与土壤全氮、有机质、含水量、pH值呈极显著正相关(P0.01),其R2依次为0.910 1,0.906 0,0.842 8,0.797 9;与土壤容重呈极显著负相关(P0.01),其R2为0.222 4;由R2值大小可知土壤养分对土壤净氮矿化量的影响程度为:土壤全氮土壤有机质土壤含水量土壤pH土壤容重。     

采用15N同位素稀释法研究不同层次土壤氮素总转化速率

采用15N同位素稀释方法,开展短期(7天)室内培养实验,估算了一水稻土0~20、20~60和60~90 cm土层土壤主要N素转化过程的总转化速率,结果表明,标记N溶液加入后2 h内各土层土壤的总矿化、硝化、固定速率显著高于其他时间段(p<0.01)。2 h后,矿化速率在小范围内起伏。0~20 cm土层土壤N素的硝化速率随培养时间延长而降低,另外两层土壤则基本保持稳定,硝化速率的变化与硝化作用底物NH4+-N浓度的变化呈显著正相关。值得注意的是,外源无机N溶液加入后2 h内,大量NH4+-N和NO3--N被固定,并认为N素的非生物固定起主导作用。2 h后,出现了N素在固定与再矿化间反复转换的现象。实验结果表明,与净转化速率相比总转化速率能更好地描述单个N素转化过程,但由于外源N加入对N素转化的影响、再矿化作用以及忽略了N素转化过程中的气体损失、DNRA(硝态氮异化还原为铵)过程等,本研究结果与真实值间存在一定差异。     

水热条件对华西雨屏区柳杉人工林土壤氮矿化的影响

采用室内培养方法研究了温度(5、15、25和35℃)和湿度(20、40、60和80%田间持水量(FWC))对华西雨屏区柳杉(Cryptomeria fortunei)人工林表层(0~20 cm)土壤氮素矿化的影响,并探讨了温度和湿度与土壤氮素矿化的关系及土壤氮素矿化的最适温度和湿度。结果表明:在30 d的培养过程中温度和湿度均对华西雨屏区柳杉林土壤氮矿化有显著影响(p0.05);相同水分条件下,土壤净氨化速率和氮净矿化速率均随温度的升高而增加;净硝化速率先随温度的升高而增加,在25℃时达到最大值,之后又随温度的升高而降低。相同温度条件下,土壤净氨化速率、净硝化速率和氮净矿化速率均先随水分含量的升高而增加,在60%FWC时达到最大值,之后又随水分含量的升高而降低。在温度和湿度16个交互处理中,35℃和60%FWC条件下土壤净氨化速率和氮净矿化速率最高,在25℃和60%FWC条件下土壤净硝化速率最高;在5℃和20%FWC条件下土壤净氨化速率、净硝化速率和氮净矿化速率最低。土壤氮净矿化的最适温度和湿度分别为32.9℃和64.1%FWC。研究区各土壤水分含量(x1)和温度(x2)条件下的氮净矿化速率(y7)可用关系式y7=-0.5374+0.05001x2+0.04374x1-0.0009228x22-0.0003749x12+0.000215x1·x2进行估算。土壤氮矿化Q10值在5~35℃内随温度的升高而降低,氮净矿化在5~15℃内对温度敏感性最高。氮净矿化作用产生的无机氮中铵态氮占77.6~87.6%,说明该区柳杉人工林表层土壤氮矿化形成的铵态氮只有少部分转化成了硝态氮,这有利于减少研究区多雨条件下矿质氮的淋失。     

模拟氮沉降对温带不同森林类型土壤氮矿化速率的影响

通过室内模拟不同氮形态(NH4+-N、NO3--N、NH4+-N+NO3--N)沉降实验,研究不同氮形态沉降对温带不同森林类型(椴树红松混交林、白桦天然次生林、红松人工林和落叶松人工林)土壤氮矿化速率的影响。结果表明:在整个培养期间,与对照相比,经过氮沉降土壤净氨化速率、净硝化速率及净矿化速率都呈现出增长趋势,而其增加的程度又取决于森林类型、土层、氮处理类型和处理时间。不同林型土壤净氨化速率、净硝化速率及净矿化速率受氮沉降影响不同,混交林对氮沉降的响应要弱于阔叶林,高于针叶人工纯林;土壤A层比土壤B层对氮沉降敏感;以铵态氮形态沉降时对铵态氮含量、净氨化速率影响较大,以硝态氮形态沉降时对硝态氮含量、净硝化速率影响较大,混合形态的氮沉降要比单种形态的氮沉降使土壤净氨化速率、净硝化速率及净矿化速率增加幅度更高;氮沉降时间越长,土壤净氨化速率、净硝化速率及净矿化速率与对照差距越大,说明氮沉降对土壤的影响存在累加效应。     

应用15N稀释法测定土壤氮素初级转化速率的一些关键技术

本文综合评述了应用~(15)N库稀释法测定土壤氮素初级转化速率的一些关键技术,即~(15)N标记土壤氮库的方法、~(15)N的加入量、丰度和标记物种类的选择,以及初始取样时间的确定。只有合理地运用这些关键技术,才能更准确地测定土壤氮素初级转化速率,进而更真实地表征土壤氮素的实际周转状况。     

Nutrient Solution and Nutrient Soil Solution Parameter Evolution in Tomato with Dynamic Fertigation under Saline Conditions

This trial was carried out to study the evolution of the nutrient parameters of the nutrient solution applied to tomato plants (Lycopersicum sculentum Mill. Forteza) cultivated in Mediterranean greenhouse conditions under different fertigation management models. The dynamic model is based on soil water content, which was measured by tensiometers, and on soil solutions obtained with suction cups (porous ceramic cup water samplers). The local traditional method consists of following technical recommendations, and the classical model requires the estimation of Crop Factor (Kc) and knowing the nutrient extraction. Nutrient solution and water applied are functions of the fertigation management criteria. The water used for fertigation was classified as C₄-S₃ according to the Riverside classification system. The cultivation period lasted from 15 August to 20 April. The nutrient parameters studied in nutrient and soil solution were pH, electrical conductivity (EC), nitrate (NO₃ ^?), phosphate (H₂PO₄ ^?), potassium (K⁺), calcium (Ca²⁺), magnesium (Mg²⁺), sodium (Na⁺), and chloride (Cl^?). The pH shows similar trends under the different treatments. Electrical conductivity is in the range of 2.8–4.5 dS m^?1. Chloride, sodium, magnesium, and sulfate are exclusively modified by the salt concentration in the irrigation water, so it can be assumed that the three treatments vary equally. Nitrate, potassium, phosphate, and calcium are modified depending on each fertigation management method. Soil solution is modified by the nutrient solution applied. Dynamic management allows low nutrient concentration in the nutrient solution to be maintained and keeps soil nutrient concentration low, reducing fertilizer losses and therefore aquifer contamination.     

A study of soil and fertilizer phosphorus intake by Barley plants,using Radioactive tracer technique

Introduction

Phosphorus applied on a phosphate deficient soil, by increasing the root development of the plant, or by stimulating the soil microflora especially in the rhizosphere, may increase the amount of phosphorus which the plant takes up from the soil. This present paper is a report on a pot culture investigation of such an effect of added phosphorus.     

Radioactive cesium distribution in bamboo [Phyllostachys reticulata (Rupr) K. Koch] shoots after the TEPCO Fukushima Daiichi Nuclear Power Plant disaster

Abstract

Radioactivity levels of cesium (Cs)-134 and ¹³⁷Cs in bamboo [Phyllostachys reticulata (Rupr) K. Koch] sprouts grown from April to June 2011 over a wide area (including Fukushima Prefecture) were elevated (max. 3100 Bq kg^?1 fresh weight) after the Tokyo Electric Power Company, Inc. (TEPCO) Fukushima Daiichi Nuclear Power Plant disaster in March 2011. Bamboo sprouts in 2012 also contained high radioactivity levels. Radioactivity imaging analysis of bamboo sprouts harvested in 2012 showed increasing concentration gradients of radioactivity from the lower parts to the top of the sprouts. The peels were individually separated from the sprouts, and the inner edible part (trunk) was cross-sectioned at the internodal sections from the top to the lower parts. Each segmented trunk and its corresponding peel were analyzed for radioactive cesium (¹³⁴Cs and ¹³⁷Cs) and stable cesium (¹³³Cs). The concentrations of ¹³⁴Cs and ¹³⁷Cs showed significant increases from the lower part to the top, whereas ¹³³Cs showed an almost constant value in the trunk and peel except in the peel of the top node. We speculated that ¹³⁴Cs and ¹³⁷Cs in newly emerging bamboo sprouts in 2012 were translocated mainly from various plant tissues (where the fallout was layered on the bamboo tissues) in older bamboo, while ¹³³Cs was translocated from the soil through the roots of the new bamboo sprouts and was present in the roots and stems.     

Activity Disequilibrium between 234U and 238U Isotopes in Southern Baltic

The aim of the work was to determine the concentration of ²³⁴U and ²³⁸U and calculate the values of the ²³⁴U/²³⁸U activity ratio in samples of living organisms, bottom water, surface and interstitial water and also sediments from the various regions of southern Baltic Sea. The knowledge of ²³⁴U/²³⁸U activity ratio in ecosystem allows getting know about the mechanisms and processes of uranium transport and origin.The activities of the analyzed uranium radionuclides in samples were measured using alpha spectrometry. The results of researches revealed diversified concentrations of uranium in the sediments of the southern Baltic Sea (sea and coastal waters) and increase of uranium with sediment depth, suggesting the diffusion of uranium from sediments to water through interstitial water and diagenesis processes in sediment material. The nuclides of uranium ²³⁴U and ²³⁸U were radioactive state equilibrium in most of the sediments. The values of the ²³⁴U/²³⁸U activity ratio oscillate around one. In bottom, surface, interstitial water and living organisms mean values of the ²³⁴U/²³⁸U activity ratio are between 1.12 and 1.15. Higher uranium concentration was observed in samples of sediments and seawater after flood in 1997 and torrential rainfalls in 2000–2001. River waters are characterized by higher ²³⁴U/²³⁸U activity ratio. The values of the ²³⁴U/²³⁸U activity ratio equal 1.27 in sediments and 1.34–1.38 in seawater indicate the influence of fresh waters.     

Die Aufnahme von137Cs und 90Sr durch verschiedene sommerrapsgenotypen aus dem Boden*

Die Aufnahme von ¹³⁷Cs und ⁹⁰Sr durch verschiedene Sommerrapsgenotypen aus kontaminiertem Boden wurde geprüft. Statistisch gesicherte Unterschiede bei der Aufnahme von Radionukliden zwischen den Genotypen konnten festgestellt werden. Die Aufnahme von ¹³⁷Cs und ⁹⁰Sr ist bei den einzelnen Sorten nicht gleichsinnig. Nur ein geprüfter Genotyp nahm sowohl geringe Mengen von ¹³⁷Cs, als auch von ⁹⁰Sr auf. Die mittleren jährlichen Schwankungen in der Radionuklidaufnahme waren größer, als die Unterschiede zwischen den Genotypen.     

Atmospheric nitrate deposition and the microbial degradation of cellobiose and vanillin in a northern hardwood forest

Human activity has increased the amount of N entering terrestrial ecosystems from atmospheric NO₃⁻ deposition. High levels of inorganic N are known to suppress the expression of phenol oxidase, an important lignin-degrading enzyme produced by white-rot fungi. We hypothesized that chronic NO₃⁻ additions would decrease the flow of C through the heterotrophic soil food web by inhibiting phenol oxidase and the depolymerization of lignocellulose. This would likely reduce the availability of C from lignocellulose for metabolism by the microbial community. We tested this hypothesis in a mature northern hardwood forest in northern Michigan, which has received experimental atmospheric N deposition (30 kg NO₃⁻-N ha⁻¹ y⁻¹) for nine years. In a laboratory study, we amended soils with ¹³C-labeled vanillin, a monophenolic product of lignin depolymerization, and ¹³C-labeled cellobiose, a disaccharide product of cellulose degradation. We then traced the flow of ¹³C through the microbial community and into soil organic carbon (SOC), dissolved organic carbon (DOC), and microbial respiration. We simultaneously measured the activity of enzymes responsible for lignin (phenol oxidase and peroxidase) and cellobiose (β-glucosidase) degradation. Nitrogen deposition reduced phenol oxidase activity by 83% and peroxidase activity by 74% when compared to control soils. In addition, soil C increased by 76%, whereas microbial biomass decreased by 68% in NO₃⁻ amended soils. ¹³C cellobiose in bacterial or fungal PLFAs was unaffected by NO₃⁻ deposition; however, the incorporation of ¹³C vanillin in fungal PLFAs extracted from NO₃⁻ amended soil was 82% higher than in the control treatment. The recovery of ¹³C vanillin and ¹³C cellobiose in SOC, DOC, microbial biomass, and respiration was not different between control and NO₃⁻ amended treatments. Chronic NO₃⁻ deposition has stemmed the flow of C through the heterotrophic soil food web by inhibiting the activity of ligninolytic enzymes, but it increased the assimilation of vanillin into fungal PLFAs.     

The use of environmental radionuclides as tracers in soil erosion and sedimentation investigations: recent advances and future developments

Although much of the recent attention on the environmental problems has focused on climatic change, there is also increasing concern that accelerated soil erosion and associated land degradation represent a major problem for sustainable development and environmental protection. There is an urgent need to obtain reliable quantitative data on the extent and rates of soil erosion worldwide to provide a more comprehensive assessment of the magnitude of the problems and to underpin the selection of effective soil conservation measures. The use of environmental radionuclides, in particular ¹³⁷Cs, affords an effective and valuable means for studying erosion and deposition within the landscape. The key advantage of this approach is that it can provide retrospective information on medium-term (30–40 years) erosion/deposition rates and spatial patterns of soil redistribution, without the need for long-term monitoring programmes. Advantages and limitations of the technique are highlighted. The launching of two closely linked International Atomic Energy Agency (IAEA) research networked projects in 1996 involving some 25 research groups worldwide has made a major contribution to co-ordinating efforts to refine and to standardise the ¹³⁷Cs technique. The efficacy and value of the approach has been demonstrated by investigations in a number of environments. Significant developments that have been made to exploit its application in a wide range of studies are reported in this review paper. Other environmental radionuclides, such as unsupported ²¹⁰Pb and ⁷Be offer considerable potential for use in soil erosion investigations, both individually and complementary to ¹³⁷Cs. The IAEA through research networks and other mechanisms is promoting further development and applications of these radionuclides in soil erosion and sedimentation studies for a sustainable resource use and environmental protection.     

Kinetics of 110mAg, 60Co, 137Cs and 54Mn Bioaccumulation from Water and Depuration by the Crustacean Daphnia Magna

As part of a research program on the transfer withinfreshwater ecosystems of the radionuclides present inthe weakly radioactive liquid effluents from nuclearpower stations, this article presents an assessment ofthe dynamics of zooplankton contamination from water.The experiments were carried out on young pre-adultdaphnids, placed at a density of 250 individualsL^-1, in 0.45 m filtered river water. Inorder to maintain the radionuclide contamination at ahigh level and to limit alterations in the chemicalcharacteristics of the medium, the water was reneweddaily. The dynamics of contamination were described bya one (¹³⁷Cs) or a two compartmental model(^110mAg, ⁶⁰Co and ⁵⁴Mn), taking intoaccount the fluctuations of the radionuclideconcentration in the water, as well as the growth rateof the daphnids. The contamination levels weredescribed by the steady state value of theconcentration factor, which was 2870 mL g^-1 wetweight, 320 mL g^-1 wet weight, 265 mL g^-1wet weight and 30 mL g^-1 wet weight, for^110mAg, ⁵⁴Mn, ⁶⁰Co and ¹³⁷Cs,respectively. During the depuration phase,radioactivity decrease in daphnids was a very fastphenomenon indicating that radionuclide adsorption tothe surface was the major process involved in thecontamination of daphnids. The two correspondinghalf-lives of the depuration kinetics were 10 min and7 d, 20 min and 1 d and 3 hr and 1.5 d for⁶⁰Co, ^110mAg and ⁵⁴Mn, respectively.The loss of ¹³⁷Cs was characterised by a singlehalf-life of 0.3 d.