抗除草剂转基因水稻的安全性评价

以转基因抗除草剂水稻（Oryza sativa ssp.japonica）为例,对转基因作物进行安全性评价.对转bar基因水稻进行了基因流动实验、小鼠急性毒性实验、致突变实验和Ames实验.结果表明,转bar基因水稻花粉在5 m内有少量的漂移到普通野生稻（O.rufipogon）,但没有向其它野生稻（O.officinalis和O.meyeriana）和杂草漂移.小鼠经口急性毒性半数致死量LD50＞20 g/kg,属实际无急性毒性物质;经微核实验、精子畸变实验及Ames实验均未发现有致突变作用,初步表明转bar基因水稻对小鼠是安全的.     

高剂量辐照鸡胸肉安全性毒理学评价

以10、15和25kGy剂量辐照处理鸡胸肉,通过动物急性毒性试验、Ames试验、骨髓细胞微核试验、精子畸形试验和30d喂养试验,对其食用安全性进行评价。结果表明,雌、雄小鼠急性经口LD50均大于10g/kg.BW,属实际无毒级;Ames试验、小鼠骨髓微核试验、精子畸形试验结果均为阴性;大鼠30d喂养试验各项指标均未见异...     

动物标本在动物学教学中的作用

动物标本是动物学教学中最好、最直观的教具和观察材料．主要种类有浸制标本、剥制标本、骨骼标本、干制标本和玻片标本等。广西大学动物学标本室保存了动物标本1350种．3276份．包括虎纹捕鸟蛛、虎纹蛙、金钱龟、蟒蛇、巨蜥、海南虎斑璃、金雕、黑熊、穿山甲、克隆牛和白头叶猴等珍稀物种。动物标本在动物学教学中的作用，主要是使动物学教学更为直观和形象．活跃动物学教学的课堂气氛．增加学生学习动物学的浓厚兴趣以及提高学生的动手能力。     

南安县域甘薯的土壤养分丰缺指标研究

应用"3414"田间肥料试验设计的试验结果,旨在了解南安甘薯产区的N、P、K肥料施用效果,建立县域土壤的甘薯养分丰缺指标,并通过多元二次肥料效应函数寻求甘薯最高产量和最佳经济施肥量。甘薯施用N、P、K肥增产增效较为显著,其增产增收效果NPKNKP,单位肥料效益PNK。南安县域内甘薯的土壤养分丰缺等级指标划分为3级,即当土壤碱解氮、有效磷、速效钾养分含量分别为75 m g/kg、20 m g/kg、65 m g/kg时为低,在75~177 m g/kg、20~40 m g/kg、65~135 m g/kg时为中,177 m g/kg、40 m g/kg、135 m g/kg为高。甘薯推荐施肥量随土壤碱解氮、有效磷、速效钾含量的提高而减少。全市平均,甘薯最高产量施肥量为N 197.43 kg/hm2,P2O563.84 kg/hm2,K2O 226.32 kg/hm2;最佳经济施肥量N 179.74 kg/hm2,P2O561.10 kg/hm2,K2O 212.50 kg/hm2。     

臭氧降解花生中黄曲霉毒素的设备及应用

为了高效降解花生中黄曲霉毒素,研制了一套臭氧降解黄曲霉毒素的设备。以人为污染的花生为试验材料,利用此设备研究了臭氧处理时间及其相对湿度对花生脱毒效果的影响。研究结果表明:臭氧能有效降解花生中的黄曲霉毒素,且臭氧处理时间和相对湿度显著影响其降解效果(P<0.05)。在臭氧浓度89mg/L、流速1L/min、搅拌速度70r/min条件下,黄曲霉毒素的较佳降解工艺为:臭氧相对湿度50%,处理时间30min。在此条件下,花生中黄曲霉毒素B1、B2、G1和G2的含量分别从87.53、21.99、9.71和4.38μg/kg降低到15.23、8.31、2.81和2.11μg/kg,降解率分别为82.6%、62.2%、71.1%和51.8%。研究结果可为花生贮藏和加工企业降低花生中的黄曲霉毒素、确保花生食用安全性提供技术参考。     

用冻干精子生产ICSI小鼠

前期研究中，我们采用小鼠卵母细胞胞浆内精子注射（ICSI）技术，成功地用新鲜精子获得了生理健康的ICSI小鼠。在此基础上，本文结合细胞冻干技术，进行了冻干精子ICSI生产试管小鼠的尝试。B6D2F1成年公鼠的精子在Tris-HCl-EDTA（pH8.2）溶液中冻干4 h后解冻，将其精子头注入到KM小鼠成熟卵母细胞的胞质中。6 h后，83.0%的卵子受精。用CZB溶液体外培养发现，冻干精子生产的胚胎，体外发育至2-C (92.0% vs 99.5%)、4-C（52.7% vs 97.2%）、桑椹胚（36.6% vs 86.3%）和囊胚的比例（21.4% vs 68.7%）极显著地（p<0.01）低于新鲜精子生产的ICSI胚胎。移植2-C期冻干精子ICSI胚胎检测体内发育，结果发现，D10的着床率为63.0%（17/27）；胎鼠的出生比例为21.7%（13/60），这些ICSI小鼠成年后的生理和生殖能力正常。试验结果说明，精子冻干后，仍能生产ICSI动物，冻干技术可以用于哺乳动物精子的保存。     

1.2%鱼藤酮微乳剂的研制

报道1.2%鱼藤酮微乳剂(M E)的制备方法。以二甲苯与乙酸乙酯(2∶3,v/v)为溶剂,CRES-LOX 3433为乳化剂,自来水为介质配制出的鱼藤酮M E,经热贮稳定性测定,各项质量指标合格;对黄曲条跳甲P hy llotreta striola ta(F abric ius)的毒力,1.2%鱼藤酮M E的LC50为503.25 m g/kg,7.5%鱼藤酮乳油(EC)的LC50值为640.22 m g/kg,M E的毒力是EC的1.27倍。     

NO合酶抑制剂 L-NAME和NO前体L-Arg对小鼠体内卵母细胞成熟的影响

摘要: 采用腹腔单独注射L-NAME(左旋硝基精氨酸甲基酯)或联合注射L-NAME和L-Arg, 取出卵母细胞体外观察，研究了NO对小鼠卵母细胞体内成熟的影响。结果表明,（1）单独注射5、10、20 mg / kg体重L-NAME均能显著抑制卵母细胞第一极体的释放(P <0.01)，尤以10 mg / kg 体重L-NAME组作用最为明显, 各剂量组对生发泡的破裂均无影响(P >0.05)；（2）10 mg / kg 体重L-NAME对卵母细胞成熟的抑制作用能被同时注射的100 mg / kg 或200 mg /kg 体重L-Arg逆转，其中以200 mg / kg体重作用最为明显;（3）L-NAME对卵母细胞畸形率的影响和培养时间有关，而对卵母细胞的存活率没有统计学影响。实验结果证明NO参与了小鼠卵母细胞的体内成熟调控。     

不同品种水稻产量形成过程的养分积累与分配特征研究

通过测定三系杂交稻"汕优63"、两系杂交稻"两优2186"和常规稻"IR64"不同器官N、P和K含量动态,探讨了3种水稻各生育期的养分积累与分配特征。结果表明:3种水稻稻株N含量均随生长过程逐渐下降,同一生育期水稻N含量以"汕优63"最高;除"汕优63"K含量在齐穗时明显上升外,3种水稻的K含量随生长过程逐渐下降;而P含量总体上在分蘖盛期最高。完熟期3种水稻的N积累量依次为24.79g/m2、15.14g/m2和14.42g/m2,P积累量为4.766g/m2、3.306g/m2和3.678g/m2,K积累量为2.439g/m22、.029g/m2和1.725g/m2。"汕优63"分配到籽粒中的N素比例为61.23%,分别比"两优2186"、"IR64"高17.97%和11.23%;分配到籽粒中的P素比例为62.97%,分别比"两优2186"、"IR64"高6.41%和9.63%;分配到籽粒中的K素比例为27.51%,分别比"两优2186"、"IR64"低3.00%和2.39%。在全生育期中,"汕优63"、"两优2186"和"IR64"的N需求量依次为31.33g/m2、22.88 g/m2、24.82g/m2,N、P、K吸收比依次为1∶0.192∶0.098、1∶0.219∶0.134和1∶0.255∶0.120。3种水稻中"汕优63"的养分生产效率最低。N、P、K生产效率"汕优63"依次为37.25kg/kg、193.80kg/kg和378.80kg/kg,"两优2186"依次为52.54 kg/kg、240.38kg/kg和392.16kg/kg,"IR64"依次为58.38kg/kg、228.83kg/kg和487.80kg/kg。三系杂交稻"汕优63"的N、P、K需求量大,积累量高,分配到籽粒中的比例亦高,与其高产特性相吻合。在保证N、P、K供给的前提下,进一步提高养分生产效率是实现"汕优63"高产、高效的关键。     

一株对小鼠具有高致病性的H5N1亚型禽流感病毒的拯救

禽流感病毒(Avianinfluenzavirus)正在逐渐获得突破种间屏障感染哺乳动物的能力,揭示其获得此种能力的分子机制已经成为禽流感病毒的研究热点。A/Chicken/Guangdong/04(H5N1)是一株高致病性禽流感病毒,同时对BALB/c小鼠也具有高致病力。实验通过反向遗传操作技术对该病毒进行拯救,获得了拯救毒R-A/Chicken/Guangdong/04(R-CG)。R-CG与其亲本毒A/Chicken/Guangdong/04(W-CG)在胚半数感染量(EID50)、细胞培养半数感染量(TCID50)、对SPF鸡和BALB/c小鼠致病力等生物学特性保持一致。即R-CG与W-CG对鸡都为高致病力,静脉接种指数分别为2.88和2.91;R-CG与W-CG一样,以106EID50鼻腔感染BALB/c小鼠后,均能引起小鼠死亡,在小鼠脑、肺、肾和脾脏中都能分离到病毒,说明拯救的病毒与亲本毒一样,都可在小鼠体内有效复制,对小鼠具有高致病性。本实验成功地拯救了A/Chicken/Guangdong/04,拯救病毒R-CG可作为背景毒株,为研究禽流感病毒突破种间屏障感染哺乳动物的分子机制奠定了实验基础。     

Protection by petaslignolide A, a major neuroprotective compound in the butanol extract of Petasites japonicus leaves, against oxidative damage in the brains of mice challenged with kainic acid

The neuroprotective effect of petaslignolide A (PA), a furfuran lignan isolated from butanol fraction of Petasites japonicus (Sieb. et Zucc.) Maxim. (Compositae) leaves, on the oxidative damage in the brain of mice challenged with kainic acid was examined using behavioral signs and biochemical parameters of oxidative stress. PA (40 mg/kg) was administered to ICR male mice through a gavage for 4 days consecutively, and on the final day, kainic acid (50 mg/kg) was administered intraperitoneally. During the 4-day treatment with PA, the body weight gain was not significantly different from that of vehicle-treated control animals. PA (40 mg/kg) alleviated the behavioral signs of kainic acid neurotoxicity and reduced the mortality (50%) by kainic acid to 12.5%. Moreover, the administration of PA restored the levels of glutathione and thiobarbituric acid-reactive substances as well as GSH-peroxidase activity in the brains of mice administered kainic acid to control levels (P < 0.05). In comparison, PA (40 mg/kg) was approximately comparable to the butanol fraction (200 mg/kg) of P. japonicus extract in reducing kainic acid neurotoxicity. On the basis of these results, PA is suggested to be a major neuroprotective agent primarily responsible for the protective action of the butanol fraction of P. japonicus extract against kainic acid-induced neurotoxicity in the brains of mice.     

仿刺参肠多糖对免疫功能的影响及抗肿瘤研究

多糖能参与机体的免疫调节,具有抗肿瘤等多种功能。本实验采用灌胃不同剂量仿刺参(Apostichopus japonicus)肠多糖的方法,研究了多糖对小鼠(Mus musculus)免疫功能的影响及其对小鼠的抗肿瘤作用。结果显示,仿刺参肠多糖对接种的H22肿瘤具有剂量依赖性的抑制作用,高剂量组(400mg/kg/d)抑瘤效果最好;胸腺指数高剂量肿瘤组最高(P<0.05),而其他各组差异不明显,脾脏指数在肿瘤组内随着剂量的增大表现为先升高再降低,而空白组呈一直上升的趋势;高剂量多糖可以显著提高荷瘤小鼠和空白小鼠的白介素2(IL-2)含量(P<0.05),对荷瘤小鼠的肿瘤坏死因子(TNF-α)含量也具有显著提升作用(P<0.05),但对空白组小鼠的TNF-α含量影响不显著;荷瘤小鼠的自然杀伤(NK)细胞活力普遍低于空白小鼠,随灌胃剂量的提升空白小鼠NK细胞活力具有上升趋势,荷瘤小鼠的NK细胞活力在中剂量组达到最高后开始下降。实验表明,仿刺参肠多糖可以促进小鼠的免疫功能,并且对小鼠体内肿瘤在一定剂量范围内具有浓度依赖性的抑制作用。     

Two-year toxicity and carcinogenicity study of methyleugenol in F344/N rats and B6C3F(1) mice

Methyleugenol (MEG) was tested for toxicity/carcinogenicity in a 2-yr carcinogenesis bioassay because of its widespread use in a variety of foods, beverages, and cosmetics as well as its structural resemblance to the known carcinogen safrole. F344/N rats and B6C3F(1) mice (50 animals/sex/dose group) were given MEG suspended in 0.5% methylcellulose by gavage at doses of 37, 75, or 150 mg/kg/day for 2 yr. Control groups (60 rats/sex and 50 mice/sex) received only the vehicle. A stop-exposure group of 60 rats/sex received 300 mg/kg/day by gavage for 53 weeks followed by the vehicle only for the remaining 52 weeks of the study. A special study group (10 animals/sex/species/dose group) were used for toxicokinetic studies. All male rats given 150 and 300 mg/kg/day died before the end of the study; survival of female rats given 150 mg/kg/day and all treated female mice was decreased. Mean body weights of treated male and female rats and mice were decreased when compared to control. Area under the curve results indicated that greater than dose proportional increases in plasma MEG occurred for male 150 and 300 mg/kg/day group rats (6 and 12 month) and male 150 mg/kg/day mice (12 month). Target organs included the liver, glandular stomach, forestomach (female rats) and kidney, mammary gland, and subcutaneous tissue (male rats). Liver neoplasms occurred in all dose groups of rats and mice and included hepatoadenoma, hepatocarcinoma, hepatocholangioma (rats only), hepatocholangiocarcinoma, and hepatoblastoma (mice only). Nonneoplastic liver lesions included eosinophilic and mixed cell foci (rats only), hypertrophy, oval cell hyperplasia, cystic degeneration (rats only), and bile duct hyperplasia. Mice also exhibited necrosis, hematopoietic cell proliferation, and hemosiderin pigmentation. Glandular stomach lesions in rats and mice included benign and malignant neuroendocrine tumors, neuroendocrine cell hyperplasia, and atrophy and in mice included glandular ectasia/chronic active inflammation. In female rats, the forestomach showed a positive trend in the incidences of squamous cell papilloma or carcinoma (combined). Male rats also exhibited kidney (renal tubule hyperplasia, nephropathy, and adenomacarcinoma), mammary gland (fibroadenoma), and subcutaneous tissue (fibroma, fibrosarcoma) lesions. Male rats also exhibited malignant mesotheliomas and splenic fibrosis. These data demonstrate that MEG is a multisite, multispecies carcinogen.     

Soy isoflavonoid aglycons genistein and daidzein Do not increase the cytochrome P-450 content of the liver microsomes of mice

Mice (4-week-old, male ddy) were fed four isonitrogenic diets for 21 days: purified diet (C diet); fermented soybean (400 mg of soy isoflavonoids/kg; FSB); fermented soybean extract (400 mg of soy isoflavonoid aglycones/kg; FSBE); C with indole-3 carbinol (I3C) (2500 mg of I3C/kg; I3C). The I3C and FSB diets significantly increased the cytochrome P-450 content of hepatic microsomes in comparison with the C diet, while the FSBE diet did not. Other mice were fed seven diets for 21 days: C; C with 100 mg or 200 mg of genistein, 100 mg or 200 mg of daidzein, or 100 mg of genistein + 100 mg of daidzein/kg; I3C diet. Genistein and daidzein did not change the liver cytochrome P-450 content. There was no synergistic effect of the combined feeding of genistein and daidzein. The increase in the cytochrome P-450 content with the FSB diet depends on chemicals other than genistein and daidzein. Genistein and daidzein do not induce cytochrome P-450.     

Design, synthesis, and insecticidal activity of novel pyrazole derivatives containing α-hydroxymethyl-N-benzyl carboxamide, α-chloromethyl-N-benzyl carboxamide, and 4,5-dihydrooxazole moieties

On the basis of commercial insecticides tebufenpyrad and tolfenpyrad, two series of novel pyrazole-5-carboxamides containing α-hydroxymethyl-N-benzyl or α-chloromethyl-N-benzyl and pyrazoles containing 4,5-dihydrooxazole moieties were designed and synthesized via the key intermediate 2-amino-1-(4-substituted) phenyl ethanol. The structures of target compounds were confirmed by (1)H NMR and elemental analysis or high-resolution mass spectrum (HRMS), and their activities against cotton bollworm (Helicoverpa armigera), diamondback moth (Plutella xylostella), bean aphid (Aphis craccivora), mosquito (Culex pipiens pallens), and spider mite (Tetranychus cinnabarinus) were tested. The results of bioassays indicated that compounds containing α-chloromethyl-N-benzyl and compounds containing 4,5-dihydrooxazole showed high insecticidal activity against cotton bollworm. Especially, stomach activities of compounds Ij, Il, and IIe were 60% at 5 mg kg(-1). Moreover, the target compounds exhibited high selectivity between cotton bollworm and diamondback moth, although both of them belong to the order Lepidoptera. Although the activities against diamondback moth were at a low level, some of the target compounds exhibited antifeedant activity. The compounds also had good activities against bean aphid, mosquito, and spider mite. The foliar contact activity of compounds Ic, Id, Ie, and IIf against bean aphid were 95, 95, 100, and 95%, respectively, at 200 mg kg(-1). The miticidal and ovicidal activities of compound IIi against spider mite were both 95% at 200 mg kg(-1). Furthermore, a trivial change at 4-position of pyrazole ring would lead to great changes in properties and activities, which can easily be deduced by comparing the activities of compounds in series I (4-chloro-pyrazole compounds) with corresponding compounds in series II (4-hydro-pyrazole compounds), especially from the miticidal and ovicidal activities of Ii and IIi against spider mite.     

亮菌多糖的分离纯化及体内抗肿瘤活性研究

本文建立高效的亮菌多糖分离纯化工艺,并研究其体内抗肿瘤活性。以市售亮菌粗粉为原料,采用水提醇沉、Sevag法脱蛋白、DEAE纤维素离子交换层析、SephadexG-100凝胶过滤层析等方法得到亮菌多糖ATPSⅡ和ATPSⅡ-2;建立小鼠S180实体瘤模型,以生理盐水和环磷酰胺为阴性和阳性对照,观察ATPSⅡ和ATPSⅡ-2在不同剂量（10mg／kg40mg／kg80mg／kg）给药10d后,对小鼠肿瘤抑制的作用。结果表明：亮菌粗粉中多糖含量为22．7g／100g;紫外光谱分析显示纯化得到的ATPSⅡ纯度接近100％;ATPSⅡ和ATPSⅡ-2对肿瘤的抑瘤率随着多糖浓度的增大而增大,其高剂量组对S180的抑瘤率分别为46．7％和51．7％,与阳性对照组相比,亮菌精制多糖ATPSⅡ和ATPSⅡ-2高剂量组抗s180效果接近于临床化疗药环磷酰胺,具有显著的抗肿瘤活性。     

Preventive effect of Thea sinensis melanin against acetaminophen-induced hepatic injury in mice

The preventive effect of Thea sinensis melanin (TSM) against overdoses of N-acetyl-p-aminophenol (NAPAP) was studied on ICR mice. Animals were given 400 mg/kg intraperitoneally (i.p.) of NAPAP, and TSM was injected i.p. in doses 10-40 mg/kg 2 h before intoxication. The protective effects were evidenced by a complete blockage of the NAPAP-induced elevation of plasma alanine aminotransferase (ALT) activity, decreased concentration of thiobarbituric acid reactive substances (TBARS) to the control level, and a partial prevention of reduced glutathione (GSH) depletion in the liver tissue. Preadministration of TSM also caused restoration of superoxide dismutase (SOD) activity and resumed content of coenzymes Q9 and Q10. TSM by itself, however, did not affect the hepatic functional parameters, including serum ALT, TBARS, GSH, SOD, or coenzymes Q in the liver. Administration of TSM caused a dose-dependent inhibition of N-nitrosodimethylamine demethylase activity with ED50 of 15.8 mg/kg. Activities of ethoxyresorufin O-dealkylase and pentoxyresorufin O-alkylase isozymes were changed insignificantly. The immune suppressive effect of NAPAP on the in vivo antibody-forming cell responses was demonstrated using ICR-sensitized mice with sheep red blood cells. The joint effect of TSM and NAPAP indicated the capability of TSM to recover immunity of the animals to the level of intact mice. Results obtained demonstrate that TSM preadministration can prevent the multiple toxic effects of NAPAP.     

Protective effect of Monascus-fermented red mold rice against alcoholic liver disease by attenuating oxidative stress and inflammatory response

Monascus purpureus NTU 568 fermented rice is reported to exhibit a wide variety of biological effects, including antitumor, antihypertriglyceridemia, antioxidant, and anti-inflammatory activities. However, its role in the pathogenesis of alcoholic liver disease remains obscure. In this study, the hepatoprotective effects of Monascus-fermented red mold rice (RMR) was evaluated in vivo using chronic alcohol-induced mice as an experimental model. The alcohol-induced mice were orally treated with RMR at 307.5 mg/kg (1-fold), 615 mg/kg (2-fold), and 1537.5 mg/kg (5-fold) for 5 weeks, whereas controls received vehicle only. Treatment with RMR significantly attenuated the increased level of serum transaminases (aspartate aminotransferase and alanine aminotransferase) and hepatic triglyceride and total cholesterol accumulation. Furthermore, RMR elevates hepatic antioxidant ability that reduced hepatic cell damage (steatosis) and decreased tissue inflammatory cytokine levels. These findings suggest that Monascus-fermented RMR may represent a novel, protective strategy against alcoholic liver disease by attenuating oxidative stress, inflammatory response, and steatosis.     

Comparison of metabolic profiling of cyanidin-3-O-galactoside and extracts from blueberry in aged mice

The metabonomics changes of plasma and brain tissue after dietary supplementation with blueberry extracts (BBE) and cyanidin-3-O-galactoside from blueberry (BBM) in aged mice were investigated by (1)H NMR technique. The mice received intragastric administration of BBE (200 mg/kg/day), BBM (50 mg/kg/day), and saline water (0.9%) for 6 weeks, respectively, in the BBE, BBM, and control groups. At the end of the experiment, plasma and brain samples were collected for NMR analysis. The results demonstrated that the level of choline in plasma from BBE and BBM groups were obviously elevated relative to the control group, whereas the levels of lactate and phosphocholine in plasma were remarkably reduced. Compared with those in the control group, the levels of choline and GABA in the brain from the BBE group were obviously increased, whereas glutamate and phosphocholine in the BBE group were significantly decreased. The level of taurine in the brain from the BBM group was particularly higher than that in the control group. These results indicated supplementation with BBE or BBM might induce similar changes of endogenous plasma and brain metabolic profiles in aged mice.     

Neuroprotective effect of rough aster butanol fraction against oxidative stress in the brain of mice challenged with kainic acid

The neuroprotective effect of the butanol fraction from the methanol extract of Aster scaber Thunb. (rough aster butanol fraction) on oxidative damage in the brain of mice challenged with kainic acid was examined using behavioral signs and biochemical parameters of oxidative stress. The rough aster butanol fraction (0.4-1.0 g/kg) was administered to ICR male mice, 6-8 weeks, through a gavage for 4 days consecutively, and on the third day, kainic acid (50 mg/kg) was ip administered. When compared to the vehicle-treated control, no significant changes in body and brain weight were observed in mice administered the rough aster butanol fraction. Administration of kainic acid only, causing a lethality of approximately 54%, resulted in a significant decrease of total glutathione level and an increase of the thiobarbituric acid-reactive substances (TBARS) value in brain tissue. When the rough aster butanol fraction was examined for neuroprotective action, the rough aster butanol fraction (0.4 g/kg) alleviated the lethality (25%) of kainic acid and the behavioral sign of its neurotoxicity. Moreover, administration of the rough aster butanol fraction at a dose of 0.4 g/kg restored the glutathione level in the cytosolic portion of brain homogenate to approximately 80% (p < 0.05). Also, the rough aster butanol fraction (0.4 g/kg) led to a significant reduction of kainic acid-induced increase of TBARS value. In addition, the glutathione peroxidase activity was restored significantly (p < 0.05) in the cytosolic portion of brain homogenate, whereas glutathione reductase activity was not. On the basis of these results, the rough aster butanol fraction is suggested to contain a functional agent to prevent oxidative stress in the brain of mice.