调亏灌溉与氮营养对玉米根区土壤水氮有效性的影响

为探索调亏灌溉与氮营养对玉米根区土壤水氮有效性的影响,采用盆栽玉米试验,研究了水分调亏时期和不同施氮量对玉米根区土壤硝态氮迁移动态和水氮利用的影响。结果表明：施氮量决定根区土壤硝态氮含量,各生育阶段的灌水量和养分吸收影响硝态氮的变化动态。调亏灌溉的玉米根区中、下层土壤硝态氮含量介于常规灌溉的高水和低水处理之间。抽穗期结束时根区中、下层土壤硝态氮含量与施氮量呈正相关关系。施氮量、调亏时期对干物质和全氮累积量影响显著。拔节期水分亏缺对干物质累积量影响最大,苗期水分亏缺影响次之,抽穗期水分亏缺影响最小。苗期亏水、高氮处理的水分利用效率最高。高氮处理的植株全氮累积量最大,是无氮处理的2.54～3.23倍。低氮调亏灌溉的氮肥表观利用效率都大于30%,比高氮调亏灌溉的高约6.6%。最佳的水氮组合为抽穗期亏水低氮处理。     

局部根区灌溉水氮耦合对设施黄瓜生长及土壤中硝态氮分布的影响

通过对设施黄瓜进行灌水量、灌溉方式、水氮根区位置的不同耦合,研究了局部根区灌溉下不同水氮耦合措施对设施黄瓜生长、土壤中硝态氮分布及累积的影响.结果表明,灌水量、灌溉方式、水氮根区供应位置对黄瓜地上部生物量及产量存在着不同的交互作用.亏缺灌溉量处理的地上部生物量及产量均低于相应灌溉方式下的正常水量处理.相同灌溉量处理条件下,交替根区灌溉的黄瓜生物量与产量显著高于两侧均水均氮处理,以正常交替水氮异区处理黄瓜地上部生物量及果实产量最大,分别达到1 143kg/hm2(干重)和1.75×105 kg/hm2(鲜重);而固定根区灌溉下,尤其在水氮异区条件下,生物量与产量则下降.在亏缺灌溉量下,交替根区灌溉处理的黄瓜生物量以及产量与常规充足灌溉处理没有显著差异.在正常灌溉量条件下,通过对局部根区灌溉下不同水氮耦合对土壤中硝态氮分布的分析表明,施氮是造成土壤中硝态氮积累的原因,土壤水分的垂向运动是影响硝态氮向下淋洗的一个主要因子.固定水氮同区、交替水氮同区处理硝态氮向下淋洗较强,水氮异区处理硝态氮向下淋洗相对较弱.交替水氮异区处理氮素主要累积在0-110 cm土层,深层累积量显著低于其他水氮耦合处理.综合黄瓜生长、土壤硝态氮淋洗等因素考虑,交替水氮异区处理是最佳的水氮耦合处理方式.     

水肥耦合条件下保护地土壤硝态氮动态变化

采用二因素三水平完全设计,通过保护地低压节点渗灌番茄小区栽培试验,研究了灌溉上限和施肥量组合处理在灌溉追肥后第1、2、35、、7 d不同土层土壤硝态氮的动态变化。结果表明,较低灌溉上限,施肥量对不同时间土壤硝态氮影响不明显;提高灌溉上限,施肥量增加影响土壤剖面分异。灌溉上限和肥料对土壤硝态氮累积量的影响除第一天外均达到5%显著水平,作用由大到小分别为肥料,水分,水肥交互作用。不同采样时间0～20 cm土层硝态氮累积百分比平均在50%以上,低灌溉上限和高施肥量组合的硝态氮累积百分比最大,高灌溉上限和高施肥量组合硝态氮累积百分比最小。     

土壤中硝态氮含量的影响因素研究

采用田间试验及人工渗滤池试验方法，研究了土壤中硝态氮含量的影响因素。结果表明，影响大田土壤中硝态氮因素很多，程度不一，其中土壤类型决定着硝态氮基础含量，是内因，而施肥及施氮量是影响硝态氮含量最大的外界因素，其次是土壤湿度和氮肥品种，土壤温度对其影响不明显。     

水氮耦合对绿洲灌区土壤硝态氮运移及甜瓜氮素吸收的影响

在地处沙漠绿洲的甜瓜种植区,研究不同水、 氮输入量对土壤氮素平衡和运移的影响,为当地甜瓜生产的水肥管理提供科学依据。通过2009、 2010连续两年田间裂区试验,研究了不同灌水量（1500、 2100、 2700、 3300 m3/hm2,以W1500、 W2100、 W2700和W3300表示）和施氮量（N 0、 120、 240、 360 kg/hm2,以N0、 N120、 N240和N360表示）对土壤硝态氮分布、 累积和甜瓜的水、 氮吸收以及产量的影响。结果表明,甜瓜收获后各处理土壤硝态氮含量在040 cm土层最高, 0200 cm土层呈现先减少后增加再减少的变化趋势,且施氮量越大,硝态氮在80120 cm土层大量累积的趋势越明显。土壤硝态氮累积量随施氮量的增加而增加,随灌水量的增加而减少,灌水量超过2700 m3/hm2 时,仅有不到53%的硝态氮留存在0100 cm土层。甜瓜产量和果实氮素吸收量随灌水量和施氮量的增加而提高,但在W3300N360处理略有下降。氮素回收率随施氮量的增加持续降低,氮收获指数以处理W2700N240最大,水分利用效率以W1500N240处理最大。W2700N240处理能够兼顾甜瓜产量,平衡氮素吸收运移与土壤中硝态氮的留存空间3个方面,是绿洲灌区甜瓜种植的高产高效的水氮输入模式。     

施肥对设施菜地土壤硝态氮累积及pH的影响

在甘肃武威市设施栽培条件下,通过田间小区试验研究了不同施肥量及肥料种类（化肥、有机肥、有机＋无机）对设施土壤硝态氮累积、硝态氮在土壤剖面运移及土壤pH值变化的影响。结果表明：施氮量和肥料种类对土壤硝态氮的累积和淋溶均有较大的影响,随施氮量的增加,土壤剖面硝态氮累积量增加,其中对0～20cm土层硝态氮累积量的影响最为显著;在同等施氮量时,单施无机肥处理（NPK）、有机无机肥减半配施处理（1/2MNPK）、单施有机肥处理（M）,在40～150cm土层硝态氮的累积量分别为267.33、211.94、125.72kg.hm-2,表明只施用化肥较有机肥、有机肥与化肥配施更易造成土壤硝态氮淋溶并在深层累积。将农户习惯施肥量（MNPK）减半后施用（1/2MNPK）对蔬菜产量没有影响,并且显著减少了硝态氮在土壤中的累积,表明当地农户设施栽培肥料施用量过高,不仅造成肥料利用率低,栽培成本高,还可能给地下水位较浅的地区带来环境污染的风险。此外,土壤硝态氮含量与pH值呈极显著负相关关系,表明硝态氮在土壤中大量累积会造成土壤pH值的下降。     

河北太行山山前平原葡萄园土壤硝态氮累积特征及影响因素

为进一步摸清农户生产实践条件下果园土壤硝态氮分布特征及影响因素,以河北太行山山前平原的保定地区葡萄园为研究对象,调查28个果园生产管理现状,测定分析葡萄园和临近农田共31个样点0—200 cm土壤硝态氮含量、累积量及主要影响因素。结果表明:葡萄生产中氮肥施用量偏高,每季平均为297 kg/hm²,过量的养分投入导致氮素在土壤中累积,0—200 cm土层硝态氮淋洗现象明显,平均累积量高达1 555 kg/hm²。不同树龄、施氮量、灌溉量水平下,土壤硝态氮含量有所不同,但均表现出随土层深度增加而增加的趋势,并且明显高于农田土壤。相关性分析表明,硝态氮累积量与树龄和施氮量均呈极显著正相关,与灌溉量呈显著负相关。通径分析表明,对土壤硝态氮累积量影响最大的因素为施氮量,其次为树龄和施肥次数,最后为灌溉量,施肥次数主要通过影响施氮量来间接影响硝态氮累积量。研究区域葡萄园氮素盈余严重,土壤硝态氮大量累积,并向深层土壤淋洗,影响该地区硝态氮累积的主要因素为施氮量、树龄和灌溉量。     

不同施氮情况下小麦玉米间作土壤硝态氮的动态变化

本文主要研究了0、210、420和630kg/hm2（NO、N1、N2和N3）4种不同施氮量对小麦玉米间作土壤硝态氮(NO-3-N)含量动态变化的影响。结果表明,0～200cm土层硝态氮的含量整体表现为N3>N2>N1>N0。各生育时期低氮水平下0～60cm土层,中、高氮水平下的0～80cm土层土壤硝态氮含量变化显著。0～60cm土层土壤硝态氮累积量随作物生育时期的变化呈“双峰”曲线,峰值分别出现在小麦挑旗期和玉米大喇叭口期,而60～200cm土层土壤硝态氮累积量的变化呈“单峰”曲线,峰值出现在玉米大喇叭口期。N0处理硝态氮累积量各生育时期变化差异较小。小麦与玉米共生期内0～200cm土层硝态氮含量表现为玉米带>小麦带,差异最大的时期为小麦灌浆期和玉米大喇叭口期。土壤硝态氮向深层的运移量随施氮量增加而增加,与N0相比,施氮后100～200cm土层硝态氮累积量小麦带增加了1053～6253kg/hm2,玉米带增加了1791～7039kg/hm2。优化氮肥施用比例,适当降低小麦播前施氮量可减小土壤硝态氮深层淋溶的风险。     

氮肥施用对黄土高原中部雨养农业区土壤硝态氮分布与累积的影响

黄土高原中部雨养农业区春小麦氮肥试验表明,氮肥施用量对土壤硝态氮的移动深度没有影响,但显著影响土壤硝态氮的含量与累积。连续施氮3年后第3季春小麦收获时,0～200cm土壤剖面中累积大量的硝态氮,其中48.96%～81.38%的总累积量和60.49%～122%的当年净累积量存在于0～110cm土层中。在确定春小麦适宜施氮量时应考虑到0～110cm土层中残留硝态氮的累积量。不施氮和氮磷比例为1:1时,连续施氮三年后土壤硝态氮总累积量最低,数量几乎相等;第3季春小麦收获后较播种时,土壤硝态氮发生亏缺。施氮量为52.5 kgN hm-2时土壤硝态氮的三年总累积率和第3季春小麦当年的净累积率居各处理之首(146.55%和138.21%)。合理的氮磷配比可有效减少土壤硝态氮的累积,不合理的氮磷配比下,低施氮量也会造成土壤硝态氮的大量累积。     

不同栽培模式及施氮对玉米-小麦轮作体系土壤肥力及硝态氮累积的影响

以在陕西关中土垫旱耕人为土区进行的连续6年定位试验为对象,研究了长期覆盖栽培及施氮量对玉米?小麦轮作体系下土壤有机质、全氮及土壤剖面硝态氮残留量和分布的影响。结果表明,不同栽培模式对土壤有机质和全氮含量的影响为覆草垄沟常规节水,其中覆草模式影响达显著水平。增施氮肥不同程度地提高了土壤有机质和全氮含量。经过12季玉米-小麦的轮作,不同栽培模式0～200cm土壤剖面硝态氮残留量为垄沟节水覆草常规,垄沟和节水栽培模式与常规栽培硝态氮累积量差异达显著水平。随种植年限和施氮量增加,0～200cm土壤中硝态氮累积量明显增加,施240kg·hm-2N(N240)处理0～200cm土壤硝态氮累积量显著高于施120kg·hm-2N(N120)处理。不同施氮量下硝态氮在0～200cm土壤剖面的分布存在差异,与不施氮(N0)和N120处理相比,N240处理下各栽培模式在120cm以下的土壤硝态氮含量随深度增加而显著增加。     

不同液土比和温度条件下塿土铵离子吸附

Effects of NH₄⁺ concentration, solution/soil ratio and temperature on NH₄⁺ adsorption were studied in a Eum-Orthic Anthrosol. The slopes of the soil NH₄⁺ adsorption isotherms and the fitted n, the coefficient for the adsorption intensity, and k, the coefficient related to adsorption capacity, of the Freundlich equation increased with increasing solution/soil ratio (SSR) and with decreasing temperature (T). For the range of experimental conditions, the value of ∂q/∂c, the rate of change of the amount of NH₄⁺ adsorbed in the soil solid phase (q) with respect to the equilibrium concentration of NH₄⁺ in soil solution (c), was 0.840, indicating that q increased with increasing c. From 2 to 45 ℃, ∂q/∂SSR, the rate of change of q with respect to SSR, decreased from 2.598 to 1.996, showing that q increased with increasing SSR, while its increasing rate decreased with temperature. From SSR 1:1 to 20:1, ∂q/∂T, the rate of change of q with respect to T, decreased from -0.095 to -0.361, indicating that q decreased with increasing temperature, and at the same time the negative effect of temperature became larger as SSR increased. Thus under the experimental conditions the order of importance in determining the amount of NH₄⁺ adsorbed in the soil solid phase was ∂q/∂SSR > ∂q/∂c > |∂q/∂T|, indicating that the greatest effect on the amount of NH₄⁺ adsorbed was with the solution/soil ratio; the equilibrium concentration of NH₄⁺ had a lesser effect; and temperature had the least effect.     

中国陕西省施有机肥黄土NH4+固定的热力学性质

Some thermodynamic properties of NH₄⁺ fixation by loess soil in plowing and clay layers are discussed. The results indicate that the four ion adsorption equations commonly used can describe the properties of NH₄⁺ fixation in these soils under constant temperature. Among the four adsorption equations, the single-surface Langmuir equation is the best. When the concentration of NH₄Cl solution is 10^-1 mol below, the Freundlich equation can be used. The changes of apparent standard free energy (ΔG°), enthalpy (ΔH°) and entropy (ΔS°) illustrate that NH₄⁺ fixation in soil is an endothermic adsorption and spontaneous reaction, and the process can be enhanced by a higher temperature and clay content in soil. The "proper value of NH₄⁺ fixation by soil (K₁ × q_m) increased with increasing clay content and temperature. The heat of NH₄⁺ fixation in soil (Q_m) confirms the conclusions made in this paper.     

陕西省几种代表性土壤NH4+吸附、解吸动力学特征研究

采用连续液流法测定了五种土壤吸附、解吸ＮＨ＾＋４的动力学性质。研究表明：（１）ＮＨ＾＋４吸附、解吸平衡时间及反应速率，平衡时的吸附、解吸量及吸附平衡常数均随土壤粘粒和ＣＥＣ不同而变化；（２）不同动力学模型及同一模型对不同土壤的拟合性不同。     

利用137Cs估算土壤侵蚀速率的定量模型

A quantitative model was developed to relate the amount of ^137Cs loss from the soil profile to the rate of soil erosion,According th mass balance model,the depth distribution pattern of ^137Cs in the soil profile ,the radioactive decay of ^137Cs,sampling year and the difference of ^137Cs fallout amount among years were taken into consideration.By introducing typical depth distribution functions of ^137Cs into the model ,detailed equations for the model were got for different soil,The model shows that the rate of soil erosion is mainly controlled by the depth distrbution pattern of ^137Cs ,the year of sampling,and the percentage reduction in total ^137Cs,The relationship between the rate of soil loss and ^137Cs depletion i neither linear nor logarithmic,The depth distribution pattern of ^137Cs is a major factor for estimating the rate of soil loss,Soil erosion rate is directly related with the fraction of ^137Cs content near the soil surface. The influences of the radioactive decay of ^137Cs,sampling year and ^137Cs input fraction are not large compared with others.     

农耕地土壤137Cs与210Pbex深度分布过程对比研究

探讨了137Cs与210Pb_ex在农耕地土壤深度分布过程的差异。基于137Cs与210Pb_ex的不同沉降过程,考虑到核素由犁耕层向犁底层的扩散,对农耕地土壤137Cs、210Pb_ex的深度分布过程进行了理论推导,并以杨凌符家庄麦田剖面的实测数据予以验证,同时讨论了实测符家庄麦田剖面137Cs、210Pb_ex深度分布的规律特征及其原因,以此阐明了137Cs与210Pb_ex在农耕地土壤深度分布过程的差异。137Cs源于大气核试爆,没有持续沉降补充,犁耕层和犁底层土壤137Cs深度分布一直处于随时间变化的非稳定态;而210Pb_ex是天然核素,存在大气沉降的持续补充,犁耕层和犁底层土壤210Pb_ex深度分布最终呈稳定态。农耕地土壤137Cs、210Pb_ex深度分布的实测值曲线与理论值曲线的差异,尤其210Pb_ex,可能与耕作深度的变化历史或土地利用(覆被)变化有关。     

黄绵土与淋溶土含水量与施肥方法对铵固定和肥料氮回收率的影响

Ammonium fixation and the effects of soil moisture and application methods on fertilizer N recovery were investigatedin two soils of Shaanxi Province, China, a Luvisol and an Entisol, through two experiments performed in the laboratoryand in a glass shelter, respectively, by using ammonium bicarbonate (NH4HCO3). The laboratory closed incubationbox experiment was conducted using the Luvisol to study NH fixation rate at soil moisture levels of 10.1%, 22.7% and 35.3% water filled pore space (WFPS). The fixed NH -N increased dramatically to 51% and 66%, 67% and 74%,and 82% and 85% 1, 2 and 36 h after fertilizer incorporation at moisture levels of 10.1% and 22.7% WFPS and 35.3% WFPS, respectively. The rapid NH fixation rates at all moisture levels could help prevent NH losses from ammonia volatilization. In the glass shelter pot experiment, N fertilizer was applied by either banding (in a concentrated strip)or incorporating (thoroughly mixing) with the Entisol and the Luvisol. An average of 74.2% of the added N fertilizerwas recovered 26 days after application to the Luvisol, while only 61.4% could be recovered from the Entisol, due tohigher NH fixation capacity of the Luvisol. The amount of fixed NH decreased with increasing WFPS. The amountof fixed NH in the incorporated fertilizer treatment was, oll average, 10% higher than that in the banded treatment.Higher NH fixation rates could prevent N loss and thus increase N recovery. The results from the Luvisol showed lowernitrogen recovery as soil moisture level increased, which could be explained by the fact that most of the fixed NH wasstill not released when the soil moisture level was low. When the fertilizer was incorporated into the soil, the recovery ofN increased, compared with the banded treatment, by an average of 26.2% in the Luvisol and 11.2% in the Entisol, whichimplied that when farmers applied fertilizer, it would be best to mix it well with the soil.     

15N标记的土壤估测某些豆科植物N2固定的温室实验

Pot experiments were carried out to estimate N2 fixation by vetch,milk vetch,sickle alfalfa and broadbean in pure stand using a ^15N-labelled soil.Winter wheat was used as the non-fixing control.The 15N-labelled soil used was prepared by growing corn-wheat-corn successively on a nearly organic-matter-free Xiashu loess supplemented with adequate amounts of (15NH4)2SO4,P,K and micronutrients,then incorporating these 15N-labelled plant materials into the soil after each havest,and allowing the plant materials to be decomposed aerobically for 410d after incorporation of the plant material of the thire crop.The 15N enrichment of wheat plant-N varied slightly with organs,with a maximum difference of 9.8%,Based on 15N enrichment of soil N inferred from the mean value of the 15N enrichment in different organs of wheat 79%-91% of total N in the tops and 67%-74% of total N in the roots of legumes studied were derived from atmosphere .Estimate by isotope dilution method was in good agreement with that by the conventional difference method provided values obtained by the latter were corrected for seed N,and also with that from the measurement of N accumulated in the tops of the legumes.     

不同铵钾比对高铵下拟南芥地上部和根系生长的影响

钾在缓解植物铵毒害的过程中起着重要的作用。本文研究了高铵(30 mmol/L)条件下,不同铵钾比(7.5︰1和150︰1)对拟南芥(Col-0)主根、侧根以及地上部生长的影响。结果表明:30 mmol/L NH4+条件下,高铵钾比(150)处理显著加重了拟南芥铵毒害现象,地上部和根系生长所受的抑制作用更为明显并导致更严重的氧化胁迫。相比低铵钾比水平,在高铵处理下,高铵钾比使得拟南芥主根伸长量降低57.4%,侧根数量减少33.3%,而地上部鲜重减轻69.9%。DAB(3,3￠-二氨基联苯胺,3,3￠-diaminobenzidine)叶片染色结果表明,不加铵处理下,外源不同钾水平(0.2和4.0 mmol/L)对拟南芥叶片的氧化胁迫作用没有显著差异;而高铵处理下,相比低铵钾比处理,高铵钾比显著增加了叶片中过氧化氢的含量,加重了其氧化胁迫。伊文思蓝(Evans blue,EB)染色结果表明,不加铵处理下,外源不同钾水平对拟南芥地上部和根部的膜透性没有显著差异,而高铵处理下,高铵钾比显著增强了拟南芥地上部和根部的膜透性,表明其对细胞的伤害程度加重。可见,高铵抑制拟南芥根系和地上部生长,高铵钾比则会加重这种抑制,其原因除了高浓度钾能减少植物对铵的吸收外,可能与高铵钾比条件加剧了植物的氧化胁迫有关。因此,适宜的铵钾比在植物应对铵毒害的过程中发挥重要作用。     

Gross fluxes of nitrogen in grassland soil exposed to elevated atmospheric pCO2 for seven years

Plant response to increasing atmospheric CO₂ partial pressure (pCO₂) depends on several factors, one of which is mineral nitrogen availability facilitated by the mineralisation of organic N. Gross rates of N mineralisation were examined in grassland soils exposed to ambient (36 Pa) and elevated (60 Pa) atmospheric pCO₂ for 7 years in the Swiss Free Air Carbon dioxide Enrichment experiment. It was hypothesized that increased below-ground translocation of photoassimilates at elevated pCO₂ would lead to an increase in immobilisation of N due to an excess supply of energy to the roots and rhizosphere. Intact soil cores were sampled from Lolium perenne and Trifolium repens swards in May and September, 2000. The rates of gross N mineralisation (m) and NH₄⁺ consumption (c) were determined using ¹⁵N isotopic dilution during a 51-h period of incubation. The rates of N immobilisation were estimated either as the difference between m and the net N mineralisation rate or as the amount of ¹⁵N released from the microbial biomass after chloroform fumigation. Soil samples from both swards showed that the rates of gross N mineralisation and NH₄⁺ consumption did not change significantly under elevated pCO₂. The lack of a significant effect of elevated pCO₂ on organic N turnover was consistent with the similar size of the microbial biomass and similar immobilisation of applied ¹⁵N in the microbial N pool under ambient and elevated pCO₂. Rates of m and c, and microbial ¹⁵N did not differ significantly between the two sward types although a weak (p<0.1) pCO₂ by sward interaction occurred. A significantly larger amount of NO₃⁻ was recovered at the end of the incubation in soil taken from T. repens swards compared to that from L. perenne swards. Eleven percent of the added ¹⁵N were recovered in the roots in the cores sampled under L. perenne, while only 5% were recovered in roots of T. repens. These results demonstrate that roots remained a considerable sink despite the shoots being cut at ground level prior to incubation and suggest that the calculation of N immobilisation from gross and net rates of mineralisation in soils with a high root biomass does not reflect the actual immobilisation of N in the microbial biomass. The results of this study did not support the initial hypothesis and indicate that below-ground turnover of N, as well as N availability, measured in short-term experiments are not strongly affected by long-term exposure to elevated pCO₂. It is suggested that differences in plant N demand, rather than major changes in soil N mineralisation/immobilisation, are the long-term driving factors for N dynamics in these grassland systems.