Nitrification Inhibitor Effects on Nitrous Oxide Emission,Nitrogen Transformation,and Maize (Zea mays L.) Yield in Loamy Sand Soil in Thailand

The nitrification inhibitors (NIs) effects on soil nitrogen (N) fates and maize yields were investigated in a loamy-sand soil in Thailand. The treatments were chemical fertilizer (CF) and CF with dicyandiamide (DCD) or neem oil at two rates of 5% and 10%. Compared to the CF plot, DCD and neem oil reduced the cumulative nitrous oxide (N₂O) emission by the equivalent of 26% and 10%, respectively (P < 0.05). DCD and neem oil had a positive effect in slowing ammonium (NH₄⁺)-conversion and prolonging NH₄⁺-N in the soil with a maximum efficiency of 45% and 30%, respectively. NO₃^–N was higher in the NI plots (P < 0.05), but the effect was less pronounced later in the growing season. Adding the NIs increased maize yields and N uptake, but was only significant (P < 0.10) for neem oil. Results indicate that applying NIs is an effective method to mitigate soil N losses and enhancing N use efficiency in a tropical, agricultural field.     

Nitrification Inhibitor,Fertilizer Rate,and Temperature Effects on Nitrous Oxide Emission and Nitrogen Transformation in Loamy Sand Soil

An incubation study investigated the effects of nitrification inhibitors (NIs), dicyandiamide (DCD), and neem oil on the nitrification process in loamy sand soil under different temperatures and fertilizer rates. Results showed that NIs decreased soil nitrification by slowing the conversion of soil ammonium (NH₄⁺)-nitrogen (N) and maintaining soil NH₄⁺-N and nitrate (NO₃^?)-N throughout the incubation time. DCD and neem oil decreased soil nitrous oxide (N₂O) emission by up to 30.9 and 18.8%, respectively. The effectiveness of DCD on reducing cumulative soil N₂O emission and retaining soil NH₄⁺-N was inconsistently greater than that of neem oil, but the NI rate was less obvious than temperature. Fertilizer rate had a stronger positive effect on soil nitrification than temperature, indicating that adding N into low-fertility soil had a greater influence on soil nitrification. DCD and neem oil would be a potential tool for slowing N fertilizer loss in a low-fertility soil under warm to hot climatic conditions.     

Effects of 4-amino 1,2,4-triazole, dicyandiamide and encapsulated calcium carbide on nitrification inhibition in a subtropical soil under upland and flooded conditions

 Nitrification inhibition of soil and applied fertilizer N is desirable as the accumulation of nitrates in soils in excess of plant needs leads to enhanced N losses and reduced fertilizer N-use efficiency. In a growth chamber experiment, we studied the effects of two commercial nitrification inhibitors (NIs), 4-amino 1,2,4-triazole (ATC) and dicyandiamide (DCD), and a commonly available and economical material, encapsulated calcium carbide (CaC₂) (ECC) on the nitrification of soil and applied NH₄ ⁺-N in a semiarid subtropical Tolewal sandy loam soil under upland [60% water-filled pore space (WFPS)] and flooded conditions (120% WFPS). Nitrification of the applied 100 mg NH₄ ⁺-N kg^–1 soil under upland conditions was retarded most effectively (93%) by ECC for up to 10 days of incubation, whereas for longer periods, ATC was more effective. After 20 days, only 16% of applied NH₄ ⁺-N was nitrified with ATC as compared to 37% with DCD and 98% with ECC. Under flooded soil conditions, nitrates resulting from nitrification quickly disappeared due to denitrification, resulting in a tremendous loss of fertilizer N (up to 70% of N applied without a NI). Based on four indicators of inhibitor effectiveness, namely, concentration of NH₄ ⁺-N and NO₃ ^–-N, percent nitrification inhibition, ratio of NH₄ ⁺-N/NO₃ ^–-N, and total mineral N, ECC showed the highest relative efficiency throughout the 20-day incubation under flooded soil conditions. At the end of the 20-day incubation, 96%, 58% and 38% of applied NH₄ ⁺-N was still present in the soil where ECC, ATC and DCD were used, respectively. Consequently, nitrification inhibition of applied fertilizer N in both arable crops and flooded rice systems could tremendously minimize N losses and help enhance fertilizer N-use efficiency. These results suggest that for reducing the nitrification rate and resultant N losses in flooded soil systems (e.g. rice lowlands), ECC is more effective than costly commercial NIs. Received: 25 May 2000     

Development of a Soil N Test for Fertilizer Requirements for Corn Production in Quebec

Corn requires high nitrogen (N) fertilizer use, but no soil N test for fertilizer N requirement is yet available in Quebec. Objectives of this research were (1) to determine the effects of soil nitrate (NO₃ ^?)-N, soil ammonium (NH₄ ⁺)-N, and N fertilizer rates on corn yields and (2) to determine soil sampling times and depths most highly correlated with yields and fertilizer N response under Quebec conditions. Soil samples were taken from 0- to 30-cm and 30- to 60-cm depths at seeding and postseeding (when corn height reached 20 cm) to determine soil NH₄ ⁺ and NO₃ ^? in 44 continuous corn sites fertilized with four rates of N in two replications using a quick test (N-Trak) and a laboratory method. The N-Trak method overestimated soil NO₃ ^?-N in comparison with the laboratory method. Greater coefficients of determination were observed for soil NO₃ ^?-N analyses at postseeding compared with seeding.     

DEVELOPMENT OF A SOIL N TEST FOR FERTILIZER REQUIREMENTS FOR WHEAT

Optimal fertilizer nitrogen (N) rates result in economic yield levels and reduced pollution. A soil test for determining optimal fertilizer N rates for wheat has not been developed for Quebec, Canada, or many other parts of the world. Therefore, the objectives were to determine: 1) the relationship among soil nitrate (NO^? ₃)- N, soil ammonium (NH ⁺ ₄)- N and N fertilizer on wheat yields; and 2) the soil sampling times and depths most highly correlated with yield response to soil NO^? ₃-N and NH ⁺ ₄-N. In a three year research work, wet and dried soil samples of 0- to 30- and 30- to 60-cm depths from 20 wheat fields that received four rates of N fertilizer at seeding and postseeding (plants 15 cm tall) were analyzed for NH ⁺ ₄-N and NO^? ₃ -N using a quick-test (N-Trak) and a standard laboratory method. Wheat yield response to N fertilizer was limited, but strong to soil NO^? ₃-N.     

氮肥基追比对小麦产量、土壤水氮分布及利用的影响

为解决区域土壤质地类型针对性氮肥施用问题,在轻壤土和黏壤土上分别设置不施氮肥,氮肥基追比3∶7,4∶6,5∶5,6∶4和7∶3处理,研究小麦产量、水氮利用效率以及土壤含水量、贮水量、NH_4~+-N、NO_3~--N动态变化规律。结果表明:轻壤质土壤氮肥基追比4∶6的处理小麦产量、水分利用效率、氮肥生产效率最高分别为8 265.3 kg/hm~2,27.6 kg/(hm~2·mm),34.4 kg/kg。黏壤质土壤氮肥基追比5∶5的处理小麦产量、水分利用效率、氮肥生产效率最高分别为8 363.2 kg/hm~2,28.3 kg/(hm~2·mm),34.8 kg/kg。小麦不同生育期各土层含水量垂直分布变化较大,轻壤质土壤含水量在9.3%～26.2%,而黏壤质为9.7%～27.6%;小麦全生育期内土壤贮水量呈先升高后降低趋势,黏壤质土壤贮水量高于轻壤质。氮素追施量越多土壤表层NH_4~+-N与NO_3~--N含量越高,且随土层加深土壤NH_4~+-N与NO_3~--N含量降低,受降水影响轻壤质土壤NH_4~+-N与NO_3~--N更易于向土层深处淋溶,成熟期黏壤质各土层的NH_4~+-N和NO_3~--N含量均多于轻壤质。说明黏壤质土壤保水保氮肥能力强于轻壤质,氮肥基追比可以适当增加。     

Comparison of Factors Affecting Soil Nitrate Nitrogen and Ammonium Nitrogen Extraction

Extraction of soil nitrate nitrogen (NO₃ ^?-N) and ammonium nitrogen (NH₄ ⁺-N) by chemical reagents and their determinations by continuous flow analysis were used to ascertain factors affecting analysis of soil mineral N. In this study, six factors affecting extraction of soil NO₃ ^?-N and NH₄ ⁺-N were investigated in 10 soils sampled from five arable fields in autumn and spring in northwestern China, with three replications for each soil sample. The six factors were air drying, sieve size (1, 3, and 5 mm), extracting solution [0.01 mol L^?1 calcium chloride (CaCl₂), 1 mol L^?1 potassium chloride (KCl), and 0.5 mol L^?1 potassium sulfate (K₂SO₄)] and concentration (0.5, 1, and 2 mol L^?1 KCl), solution-to-soil ratio (5:1, 10:1, and 20:1), shaking time (30, 60, and 120 min), storage time (2, 4, and 6 weeks), and storage temperature (?18 ^oC, 4 ^oC, and 25 ^oC) of extracted solution. The recovery of soil NO₃ ^?-N and NH₄ ⁺-N was also measured to compare the differences of three extracting reagents (CaCl₂, KCl, and K₂SO₄) for NO₃ ^?-N and NH₄ ⁺-N extraction. Air drying decreased NO₃ ^?-N but increased NH₄ ⁺-N concentration in soil. Soil passed through a 3-mm sieve and shaken for 60 min yielded greater NO₃ ^?-N and NH₄ ⁺-N concentrations compared to other treatments. The concentrations of extracted NO₃ ^?-N and NH₄ ⁺-N in soil were significantly (P < 0.05) affected by extracting reagents. KCl was found to be most suitable for NO₃ ^?-N and NH₄ ⁺-N extraction, as it had better recovery for soil mineral N extraction, which averaged 113.3% for NO₃ ^?-N and 94.9% for NH₄ ⁺-N. K₂SO₄ was not found suitable for NO₃ ^?-N extraction in soil, with an average recovery as high as 137.0%, and the average recovery of CaCl₂ was only 57.3% for NH₄ ⁺-N. For KCl, the concentration of extracting solution played an important role, and 0.5 mol L^?1 KCl could fully extract NO₃ ^?-N. A ratio of 10:1 of solution to soil was adequate for NO₃ ^?-N extraction, whereas the NH₄ ⁺-N concentration was almost doubled when the solution-to-soil ratio was increased from 5:1 to 20:1. Storage of extracted solution at ?18 °C, 4 °C, and 25 °C had no significant effect (P < 0.05) on NO₃ ^?-N concentration, whereas the NH₄ ⁺-N concentration varied greatly with storage temperature. Storing the extracted solution at ?18 ^oC obtained significantly (P < 0.05) similar results with that determined immediately for both NO₃ ^?-N and NH₄ ⁺-N concentrations. Compared with the immediate extraction, the averaged NO₃ ^?-N concentration significantly (P < 0.05) increased after storing 2, 4, and 6 weeks, respectively, whereas NH₄ ⁺-N varied in the two seasons. In conclusion, using fresh soil passed through a 3-mm sieve and extracted by 0.5 mol L^?1 KCl at a solution-to-soil ratio of 10:1 was suitable for extracting NO₃ ^?-N, whereas the concentration of extracted NH₄ ⁺-N varied with KCl concentration and increased with increasing solution-to-soil ratio. The findings also suggest that shaking for 60 min and immediate determination or storage of soil extract at ?18 ^oC could improve the reliability of NO₃ ^?-N and NH₄ ⁺-N results.     

螃蟹对闽江河口互花米草湿地土壤活性养分变化的影响

为阐明螃蟹活动对湿地土壤活性养分含量变化的影响,对闽江河口湿地不同潮滩螃蟹干扰下的土壤DOC、MBC、NH_4~+-N、NO_3~--N、Fe及其价态含量特征进行测定和分析。结果表明:有螃蟹组土壤DOC和MBC含量分别为95.98,11.13mg/kg,对照组含量分别为106.99,7.54mg/kg,螃蟹组土壤DOC含量略低于对照组(P0.05),螃蟹组土壤MBC含量高于对照组(P0.05);两者含量最高值和最低值分别出现在夏季和冬季,且夏季显著高于其他季节(P0.05)。螃蟹组土壤NH_4~+-N和土壤NO_3~--N含量分别为22.45,1.08mg/kg,对照组含量分别为23.65,1.44mg/kg,螃蟹组土壤NH_4~+-N含量低于对照组(P0.05),螃蟹组土壤NO_3~--N含量低于对照组(P0.05);不同季节,土壤NH_4~+-N和NO_3~--N含量存在显著差异(P0.01)。螃蟹组土壤总铁含量略高于对照组(P0.05);螃蟹组土壤Fe2+含量显著高于对照组(P0.05);螃蟹组和对照组土壤Fe3+含量差异不明显(P0.05)。不同季节,土壤总Fe、Fe~(2+)和Fe~(3+)含量存在显著差异(P0.01)。     

Soil inorganic nitrogen composition and plant functional type determine forage crops nitrogen uptake preference in the temperate cultivated grassland,Inner Mongolia

ABSTRACT

Plant nitrogen (N)-acquisition strategy affects soil N availability, community structure, and vegetation productivity. Cultivated grasslands are widely established to improve degraded pastures, but little information is available to evaluate the link between N uptake preference and forage crop biomass. Here an in-situ ¹⁵N labeling experiment was conducted in the four cultivated grasslands of Inner Mongolia, including two dicots (Medicago sativa and Brassica campestris) and two monocots (Bromus inermis and Leymus chinensis). Plant N uptake rate, shoot- and root biomass, and concentrations of soil inorganic-N and microbial biomass-N were measured. The results showed that the root/shoot ratios of the dicots were 2.6 to 16.4 fold those of the monocots. The shoot N concentrations of the dicots or legumes were 40.6% to 165% higher than those of the monocots or non-legumes. The four forage crops in the cultivated grassland preferred to uptake more NO₃^?-N than NH₄⁺-N regardless of growth stages, and the NH₄⁺/NO₃^? uptake ratios were significantly lower in the non-legumes than in the legumes (p < 0.05). Significant differences in the NH₄⁺-N rather than NO₃^?-N uptake rate were observed among the four forages, related to plant functional types and growth stages. The NH₄⁺ uptake rate in the perennial forages exponentially decreased with the increases in shoot-, root biomass, and root/shoot ratio. Also, the plant NH₄⁺/NO₃^? uptake ratio was positively correlated with soil NH₄⁺/NO₃^? ratio. Our results suggest that the major forage crops prefer to absorb soil NO₃^?-N, depending on soil inorganic N composition and belowground C allocation. The preferential uptake of NO₃^?-N by forages indicates that nitrate-N fertilizer could have a higher promotion on productivity than ammonium-N fertilizer in the semi-arid cultivated grassland.     

厌氧土壤灭菌不同添加物对日光温室土壤性质及微生物群落的影响

厌氧土壤灭菌(ASD)作为替代化学农药熏蒸灭菌技术在各地逐渐推广,但不同土壤类型、不同添加物厌氧土壤灭菌效果具有较大差异,田间条件下明确当地不同添加物厌氧灭菌对土壤性质及微生物群落效应,为日光温室绿色环保的土壤灭菌方法提供科学依据。结果表明:(1)除添加碳酸氢铵(AB)处理外,其他处理均可以显著降低0—20 cm土层电导率(EC),但仅灌水不添加物料(CK)处理20—40 cm土层NO_3~--N和EC显著增加,且AB处理显著增加0—20 cm土层NH_4~+-N及20—40 cm土层NO_3~--N;添加鸡粪(CM)处理0—20 cm土层NH_4~+-N极显著增加,NO_3~--N极显著降低,EC显著降低,土壤有机质、全氮和有效养分亦显著增加。(2)添加鸡粪(CM)显著降低细菌丰富度和均匀度,但土壤中植物促生菌(PGPR)芽孢杆菌属(Bacillus)及假单胞菌属(Pseudomonas)的相对丰度极显著增加。对真菌群落,不同处理真菌丰富度和均匀度与处理前差异均不显著,但添加碳酸氢铵(AB)、木醋液(PS)和鸡粪(CM)处理病原菌-镰刀菌属(Fusarium)的相对丰度均显著降低;同时,鸡粪(CM)处理有益属曲霉属(Aspergillus)丰度显著增加。(3)综合土壤理化性质、细菌和真菌群落变化,鸡粪作为有机碳源添加厌氧土壤灭菌效果较好,既可以厌氧灭菌同时也腐熟鸡粪,且各地原料来源方便,可同时实现化肥农药双减。     

Effects of application of lime nitrogen and dicyandiamide on nitrous oxide emissions from green tea fields

Abstract

The aim of this study was to assess the mitigating effects of lime nitrogen (calcium cyanamide) and dicyandiamide (DCD) application on nitrous oxide (N₂O) emissions from fields of green tea [Camellia sinensis (L.) Kuntze]. The study was conducted in experimental tea fields in which the fertilizer application rate was 544 kg nitrogen (N) ha^?1 yr^?1 for 2 years. The mean cumulative N₂O flux from the soil between the canopies of tea plants for 2 years was 7.1 ± 0.9 kg N ha^?1 yr^?1 in control plots. The cumulative N₂O flux in the plots supplemented with lime nitrogen was 3.5 ± 0.1 kgN ha^?1, approximately 51% lower than that in control plots. This reduction was due to the inhibition of nitrification by DCD, which was produced from the lime nitrogen. In addition, the increase in soil pH by lime in the lime nitrogen may also be another reason for the decreased N₂O emissions from soil in LN plots. Meanwhile, the cumulative N₂O flux in DCD plots was not significantly different from that in control plots. The seasonal variability in N₂O emissions in DCD plots differed from that in control plots and application of DCD sometimes increased N₂O emissions from tea field soil. The nitrification inhibition effect of lime nitrogen and DCD helped to delay nitrification of ammonium-nitrogen (NH₄⁺-N), leading to high NH₄⁺-N concentrations and a high ratio of NH₄⁺-N /nitrate-nitrogen (NO₃^–-N) in the soil. The inhibitors delayed the formation of NO₃^–-N in soil. N uptake by tea plants was almost the same among all three treatments.     

不同培肥措施对采煤塌陷区复垦土壤氮素矿化的影响

为揭示不同培肥措施对采煤塌陷区复垦土壤氮素矿化特征,采用间歇淋洗好气培养法,研究了不施肥(CK)、单施化肥(CF)、单施有机肥(M)、化肥配施有机肥(MCF)和化肥配施生物有机肥(MCFB)5种培肥措施对复垦4年和8年土壤矿质态氮和可溶性有机氮变化及氮素矿化特征的影响。结果表明:间歇淋洗好气培养过程中,复垦8年土壤累积NO_3~-—N产生量较4年增加2.63%~26.83%,NH_4~+—N累积产生量增加12.50%~32.14%,可溶性有机氮(SON)累积产生量在CF和M处理下减少31.59%~62.50%,其他处理增加3.44%~34.69%。同一复垦年限下NO_3~-—N、NH_4~+—N和SON累积产生量均以MCFB最高。从矿化参数来看,5种培肥措施土壤矿化势(N_0)均表现为复垦8年高于复垦4年土壤,增加幅度为MCFB(26.9%)CK(15.9%)CF(15.0%)M(12.7%)MCF(4.8%);CK和CF处理下潜在可矿化有机氮(N_0/N)随复垦年限增加而减少,减少率分别为9.4%和13.8%,其余3种培肥措施N_0/N呈增加趋势,增加率表现为MCF(13.2%)MCFB(7.5%)M(2.8%);MCF处理下矿化率(N_t/N)随复垦年限增加而增加,增加率为13.2%,其余4种培肥措施的矿化率(N_t/N)均表现为复垦8年低于复垦4年土壤,减少幅度为CF(12.1%)CK(9.4%)MCFB(7.5%)M(2.7%)。不同处理间N_0、N_0/N和N_t/N值则均以8年复垦土壤的MCFB处理最高。综合来看,经过连续8年复垦,化肥配施生物有机肥较其他培肥措施更大程度上提高了各项矿化参数值,但随着复垦年限的增加,以化肥配施有机肥处理的N_0、N_0/N的增加幅度最大,以化肥配施生物有机肥处理的N_t/N减少率最低,长期单施化肥有降低潜在可矿化有机氮量(N_0/N)的趋势。     

干污泥和堆肥污泥施用于红壤中N素的释放特性

在红壤自然状况下,模拟了施肥沟,对红壤不同污泥施肥处理的N素释放特性进行了研究。试验结果表明,干污泥配比在10%~20%时,碱解氮、铵态氮和硝态氮累计释放量分别为:25.71%~33.48%,9.57%~14.85%和4.08%~7.65%。堆肥污泥配比在20%~33%时,其累计释放量分别为13.55%~15.65%,2.03%~4.23%和3.11%~5.37%。干污泥处理的释放量大于堆肥污泥处理的释放量,释放过程变化较堆肥污泥剧烈,铵态氮和硝态氮均有明显峰值,铵态氮最大含量532.98±10 mg/kg,释放量最大达10.95%;硝态氮含量最大为149.2±14 mg/kg,释放量最大时为3.32%。无论是从氮的肥效角度,还是氮释放的环境风险角度考虑,污泥堆肥处理后施肥方式均优于干污泥处理施肥方式。     

间伐对杉木人工林土壤碳氮及其组分特征的影响

为探究不同间伐强度对杉木人工林土壤碳氮及其组分特征的影响,以福建省三明市官庄国有林场11年生杉木(Cunninghamia lanceolata)人工林为研究对象,采用弱度间伐(LIT)、中度间伐(MIT)、强度间伐(HIT)等3种间伐强度,研究不同间伐强度林分0—10,10—20,20—40,40—60,60—80,80—100 cm土层总有机碳(SOC)、全氮(TN)及易氧化有机碳(ROC)、硝态氮(NO_3~--N)、铵态氮(NH_4~+-N)、微生物量碳(MBC)、微生物量氮(MBN)、微生物熵碳(qMBC)、微生物熵氮(qMBN)的变化特征,以探讨不同间伐强度对杉木人工林土壤碳氮及其组分特征的影响。结果表明:间伐降低了土壤SOC和TN的含量,降低幅度分别为1.4%～36.9%,3.1%～45.7%。间伐增加了土壤MBC、NO_3~--N的含量,而对ROC、NH_4~+-N和MBN的程度在不同土层有差异,qMBC和qMBN随着间伐强度的增加而增大。相关性分析表明,土壤SOC分别与TN、qMBC、ROC、NH_4~+-N、MBC、MBN呈极显著正相关(P0.01);TN与qMBN、ROC、NH_4~+-N、MBC、MBN呈极显著正相关(P0.01)。杉木人工林间伐处理降低了土壤表层SOC和TN含量,增加了土壤SMBC和qMBC、qMBN,同时也增加了土壤表层(0—10 cm)SMBN。抚育间伐导致土壤SOC和TN含量降低主要是由于活性碳、氮含量的增加,提高土壤中有机质分解速率,最终导致土壤SOC和TN含量降低。     

Short-Term Soil Responses for an Emulated Loblolly Pine Silvopasture

Pine (Pinus spp. L.) stands are often overstocked early in the tree rotation, prior to initial thinning. While pre- and/or post-thinning fertilizer applications are best-management practices to optimize growth of southern pines, there can be poor nitrogen (N) utilization and adverse environmental impacts associated with fertilization. Our objective was to determine short-term (3-year) soil responses of an emulated loblolly pine (P. taeda L.) silvopasture, which received a single application of commercial N–phosphorus (P)–potassium (K) fertilizer (CF) or pelletized poultry litter (PPL) applied at about midrotation (12 years postplanting). Compared to the control, CF decreased soil pH at depths of 0–10 and 10–30 cm, and PPL increased Mehlich 3 available P at 0–10 cm. Fertilizer responses were found for soil extractable ammonium (NH₄ ⁺)-N, and nitrate (NO₃ ^?)-N concentrations, mineral N ha^?1, pH, and available P but not for diel carbon dioxide–carbon (CO₂-C) flux, total C, and total N. Total soil C, total soil N, pH, and available P decreased with depth, whereas mineral N ha^?1 and 1 M potassium chloride (KCl)–extractable aluminum (Al) increased with depth. These results further our understanding of the nutrient dynamics during alley cropping of an upland soil and demonstrate the challenge in detecting short-term responses with fertilization.     

减氮配施有机物质对土壤氮素淋失的调控作用

采用室内土柱模拟试验方法,研究不同氮肥施用下1m土体中氮素的分布和移动特征,揭示土壤氮素动态变化规律。结果表明:FN(农民习惯施无机氮用量)、RN(根据土壤养分供应和作物需求确定的推荐无机氮用量)显著增加了土壤上层NH_4^+-N和NO_3^--N向下层淋失。RN+HA(与推荐无机氮纯养分相等的锌腐酸尿素)和RN40%+OMB(推荐无机氮肥减60%基础上配施自制有机调理物质)可延长上层土壤NH_4^+-N峰值出现时间,降低下层NH_4^+-N。淋溶结束后,等氮量下增施HA较RN降低60cm以下NH_4^+-N残留29.7%～54.2%;降低60—80cm NO_3^--N累积17.4%。RN40%+OMB处理无机氮肥用量最小,0—20cm的NH_4^+-N最高,40—100cm稳定在2.0mg/kg左右;0—20,20—40cm土层NO_3^--N较RN+HA增加12.3%和2.0%,显著降低40cm以下NO_3^--N残留。RN+HA和RN40%+OMB较RN的土壤总无机氮残留分别减少7.4%和20.2%,降低表观淋失率。因此,RN40%+OMB可较好地抑制氮素下移,降低氮素淋失风险,为减少氮素淋失、明确合理氮肥施用方式提供科学依据。     

Corn (Zea mays L.) Grain and Stover Yield as Affected by Soil Residual Mineral Nitrogen

Evaluation of nitrogen (N) dynamic in soil using regression equations is important for proper determination of N fertilization. A 3-year field experiment was conducted to (1) develop the best-fitted regression model relating corn grain and stover yield to soil residual ammonium (NH₄)-N and nitrate (NO₃)-N for corn yield prediction and (2) evaluate how such a model can be beneficial to the health of ecosystem by predicting the appropriate rates of N fertilization for corn production. Soil NH₄-N and NO₃-N were determined at corn harvest at the depths of 0–30 and 30–60 cm. Nitrogen fertilizer rates and soil mineral N accounted for a maximum of 93% variation in corn grain yield. Soil mineral N enhanced corn yield more than N fertilizer. Totals of 63.1 and 14.1 kg/ha of soil residual NO₃-N and NH₄-N were found in the 0- to 60-cm depth, indicating the importance of performing soil N tests.     

间歇性降雨对黄土坡地水土养分流失的影响

坡面水土养分流失是研究农业非点源污染方面的核心问题,涉及土壤侵蚀、坡地水文和环境治理等方面的内容。以黄土坡地为研究对象,利用人工降雨模拟试验,分析间歇降雨时坡地产流-入渗-土壤侵蚀过程,以及通过预先在坡地喷施养分(NH_4~+-N、NO_3~--N、PO_4~(3-)-P),研究间歇降雨时坡面水土流失以及土壤溶质的迁移规律。试验采用针孔式人工模拟降雨器进行模拟降雨,对试验坡地间歇性进行3次降雨,雨强恒为100mm/h,每次降雨历时60min,降雨间隔时间60min。结果表明:(1)3次降雨的初始含水率不同,但产流规律相似,降雨径流率均为先增大后趋于平稳。(2)3次降雨产生的泥沙累积量分别为250.91,100.20,79.76g,第1次降雨的泥沙量远高于第2,3次。泥沙率先迅速增大到峰值然后缓慢减少,平均泥沙率随降雨次数的增多而递减。(3)对于非吸附性的NO_3~-、NH_4~+,3场降雨过程中溶质浓度均呈现由高降低并逐渐平稳的变化趋势;PO_4~(3-)-P浓度的变化规律却略显不同,降雨初期溶质浓度先短暂升高,然后再由高降低并逐渐平稳。(4)3次降雨的NH_4~+-N、NO_3~--N、PO_4~(3-)-P的径流总流失量分别为535.33,1 058.18,400.79mg,其中NO_3~--N流失量最多,PO_4~(3-)-P流失量最少。随着降雨次数的增加,不同降雨次数下的NH_4~+-N、NO_3~--N、PO_4~(3-)-P径流流失量均逐渐减少,流失量较前次降雨分别降低了19%,14%、3%,62%和57%,28.3%。因此,通过对间歇性降雨条件下黄土坡地水土溶质迁移特征的研究,对揭示降雨-径流-土壤相互作用过程和土壤养分迁移机理具有重要意义。     

Soil NO3-N production and immobilization affected by NH4-N,glycine, and NO3-N addition in different forest types in Shikoku,southern Japan

The characteristics of production and immobilization of NO₃-N were evaluated for soils from four forest types in Kochi Prefecture, southern Japan. Net NO₃-N production during the laboratory incubation differed among the soils from the four forest types, being high under Japanese cedar (Cryptomeria japonica D. Don) and deciduous hardwood, and negligible under Japanese red pine (Pinus densiflora Sieb. et Zucc.) and hinoki cypress (Chamaecyparis obtusa Endlicher). Nitrification under Japanese cedar and hardwood was mainly autotrophic based on the fact that nitrification was inhibited by acetylene or nitrapyrin, and was not affected by cycloheximide. Net NO₃-N production in these soils increased by glycine addition, but did not increase appreciably by NH₄Cl addition. However, net NO₃-N production increased after the addition of CaCO₃ with NH₄Cl. These results indicate that the substrate of nitrification is NH₃ rather than NH₄ ⁺ and that the added NH₄ ⁺ is not utilized by nitrifiers at low pH values. With NO₃-N addition to soils under red pine and hinoki cypress, immobilization of NO₃-N was observed followed by rapid production of NH₄-N. These findings suggested that mobile NO₃-N can be converted to less mobile NH₄-N by the activities of soil microorganisms. This microbial process may play an important role in retaining nitrogen within forest ecosystems where the potential of N loss is high due to the high precipitation in the area.     

不同水氮条件下双氰胺(DCD)对温室黄瓜土壤氮素损失的影响

【目的】设施蔬菜习惯"大水大肥"的传统管理模式,不仅影响蔬菜的品质和产量,造成严重的资源浪费,而且引起的环境污染问题日益受到人们的关注。本研究针对设施蔬菜生产中过量施用氮肥以及不合理的灌溉所导致的氮肥利用率低、氮素损失等资源浪费和环境的负效应问题,重点研究双氰胺(DCD)在设施蔬菜生产体系中的硝化抑制效果及其影响机制,并筛选出了适用于设施黄瓜生产的最优水氮管理方案。【方法】采用田间原位跟踪法,对温室黄瓜追肥期间土壤N2O排放量、氨挥发损失量、无机氮含量等指标进行了测定。N2O气体样品用密闭式静态箱法采集,用Agilent GC6820气相色谱仪进行测定。氨挥发样品用密闭室法采集,硼酸溶液吸收,标准硫酸滴定法测定。新鲜土样用1.0 mol/L KCl浸提,滤液用TRACCS 2000型流动分析仪测定土壤的NH+4-N和NO-3-N含量。【结果】在不同水氮条件下[传统水氮(T)的施氮量为N 988.6 kg/hm2、灌溉量为758.8 t/hm2;推荐水氮Ⅰ(R1)的施氮量为N 709.4 kg/hm2,推荐水氮Ⅱ(R2)的施氮量为N 746.9 kg/hm2,灌溉量均为531.2 t/hm2]。加施DCD后,推荐水氮Ⅰ、推荐水氮Ⅱ处理N2O的排放通量分别显著减少了42.1%和64.1%,但氨挥发损失分别显著增加了34.3%和40.4%;0—10 cm土层土壤硝态氮与N2O排放通量呈极显著的正相关,铵态氮与氨挥发损失呈极显著正相关。传统水氮处理在0—60 cm土壤剖面均检测到大量的硝态氮,前两次追肥后尤为明显。在减氮基础上加施DCD有助于减少硝态氮的累积,对0—30 cm根区硝酸盐淋洗的抑制作用较为明显。在0—30 cm土壤-蔬菜体系中,传统水氮处理的氮素表观损失显著高于其他施氮处理。加施DCD后,推荐水氮Ⅰ、推荐水氮Ⅱ处理的氮素盈余和氮素损失率均有所降低。与传统水氮处理相比,推荐水氮Ⅱ+DCD的处理增产23.3%,经济效益增加25560yuan/hm2。【结论】在本试验条件下,适度减氮控水措施是切实可行的,不仅满足了作物生长所需要的氮素,而且减少了氮素的盈余,提高了氮素的利用率,且不影响作物产量。在控水灌溉条件下,推荐施氮Ⅱ+DCD(氮素用量的15%)不仅能减少土壤氮素的盈余量,而且可有效地增加经济效益和环境效益。