土区长期施肥对小麦-玉米轮作体系钾素平衡与钾库容量的影响

本文研究了土区小麦-玉米轮作体系长期氮磷钾化肥不同配合施用方式及氮磷钾化肥与秸秆或有机肥配合施用对钾素平衡以及土壤钾库的影响。试验包括9个处理,分别为不施肥（CK）、单施氮（N）、氮钾（NK）、磷钾（PK）、氮磷（NP）、氮磷钾（NPK）、氮磷钾配合一季秸秆还田（SNPK）、氮磷钾配合低量有机肥（M1NPK）和高量有机肥（M2NPK）。结果表明,除NK、PK和M2NPK处理外,其它处理小麦和玉米钾的携出量均大于钾的投入量,导致土壤钾素处于亏缺状态,20年累计亏缺量为6174333 kg/hm2。与试验前相比,长期施肥种植没有显著影响土壤全钾含量; 长期施用钾肥显著提高土壤速效钾含量,但长期不施钾肥处理的土壤速效钾含量也未显著降低; 无论施钾与否土壤非交换性钾（Mactotal K）以及非交换性钾中更容易被HNO3溶解提取的钾（Step K）均明显低于试验前水平。表明土壤非交换性钾可以作为该土壤钾素消耗的指标。考虑到施钾肥的经济投入和现有资源高效利用（如秸秆、有机肥）,从长远的角度出发,维持土壤钾素肥力以及土地可持续生产力,土区小麦-玉米轮作体系采用秸秆全部还田或施有机肥是必要的。     

长期施钾对红壤水稻土水稻产量及土壤钾素状况的影响

研究了长期定位施肥试验中连续27年（1981～2007）施用钾肥对水稻产量和土壤钾素状况的影响。本试验选择了其中5个处理:CK（不施肥）、NP（施氮、磷肥）、NPK（施氮、磷、钾肥）、NP+RS（施氮、磷肥和稻草）和NPK+RS（施氮、磷、钾肥和稻草）。结果表明,施钾能明显提高水稻产量,施钾肥的NPK和NPK+RS处理27年的早稻平均产量分别比NP和NP+RS处理增加15.2%和10.9%;晚稻增产17.2%和9.1%;在27年54季水稻种植期间,不同施肥处理早、晚稻产量的变化趋势不同。CK、NP处理的早、晚稻产量随时间的推移呈负变化趋势,而NPK、NP+RS和NPK+RS处理的早、晚稻产量呈正变化趋势。施钾的NPK和NPK+RS处理不同土层中土壤全钾、缓效钾和速效钾含量均高于NP和NP+RS处理的相同土层;除NPK+RS处理外,其它各处理表观钾平衡均为负值,其中CK和NP处理钾的负平衡值最大。长期施用钾肥能提高水稻产量和维持土壤钾素肥力。     

长期施肥对塿土冬小麦产量及土壤养分的影响

以20年塿土冬小麦-夏休闲轮作体系长期肥料定位试验为平台,探讨长期施肥对小麦产量及土壤养分的影响。结果表明:同时施入氮磷肥处理(NP、NPK、MNPK)小麦产量上升趋势显著,年均增产幅度为219~265 kg hm~(-2),平均产量均为CK的3倍。化肥配施有机肥(MNPK)土壤有机质、全氮、全磷、速效磷和速效钾含量均呈明显上升趋势,年均分别增加0.93g kg~(-1)、0.05 g kg~(-1)、0.07 g kg~(-1)、12.11 mg kg~(-1)、8.8 mg kg~(-1),有机质和全磷的平均含量较CK分别提升59%、73%,速效磷和速效钾平均含量为CK的13.6倍和1倍。氮磷配施(NP、NPK)土壤有机质平均含量较CK增加18%和16%。施氮肥对土壤中全氮含量没有明显影响。施磷肥(NP、PK、NPK)土壤全磷以及速效磷含量逐年显著上升,土壤全磷平均含量较CK提升27%,速效磷含量为CK的4倍。施钾肥(NPK、NK、PK)对土壤全钾含量没有明显影响,但土壤速效钾上升趋势显著,年均增幅为5.6~6.9 mg kg~(-1),平均含量较CK分别增加38.8%、59.3%和48.1%。旱作塿土合理的施肥措施为有机肥配合氮肥。     

长期施钾与秸秆还田对华北潮土和褐土区作物产量及土壤钾素的影响

分析了在华北平原的河北潮土和山西褐土上连续13年施用钾肥和秸秆还田下的大田作物产量和耕层土壤钾素状况。结果表明,在施用氮磷肥基础上长期施钾和秸秆还田能增加小麦和玉米产量,各施钾处理产量与氮磷处理差异显著。两定位点钾肥和秸秆还田的效应不尽相同;小麦年际间各处理产量变异（CV≤13%）小于玉米（CV14%）,河北潮土上的玉米增产效果高于小麦。与山西单作制度相比,河北轮作制度下土壤钾素支出大。秸秆还田和施用钾肥较只施氮磷肥可不同程度提高河北潮土和山西褐土0-20和20-40 cm两土层水溶性钾 （河北0-20 cm平均提高5.8 mg/kg,山西16.9 mg/kg,下同）、非特殊吸附钾（21.2 mg/kg,35.9 mg/kg）、非交换性钾（75.1 mg/kg,57.5 mg/kg）、矿物钾（0.03％,0.01％）及全钾含量;降低矿物钾比例的同时提高其余几种形态钾的比例。随土层加深,除矿物钾外,其余形态钾含量和比例均下降,特殊吸附钾不受施钾措施和土层深度的影响。直接施用钾肥效果优于秸秆还田,且两种措施对上层土壤各形态钾的影响效果大于下层土。     

长期定位施肥对旱作塿土小麦产量、养分效率及养分平衡的影响

本文以20年塿土冬小麦-夏休闲轮作体系长期肥料定位试验为平台,探讨小麦产量、肥料利用效率及养分平衡对长期不同施肥模式的响应。试验包括7种施肥模式:不施肥(CK)、单施氮肥(N)、氮磷(NP)、氮钾(NK)、磷钾(PK)、氮磷钾(NPK)、有机肥与氮磷钾化肥配施(MNPK)。结果表明,同时施入氮肥和磷肥处理的小麦产量上升趋势显著,年均增产幅度为219~265 kg hm-2。施氮肥处理土壤氮素盈余,施磷肥处理土壤磷素盈余。以N和NK氮素盈余最多,年均盈余分别为88 kg hm-2和66 kg hm-2,MNPK土壤磷素盈余达到107 kg hm-2。同时施入氮磷肥处理(NP、NPK)显著提高了氮(磷)肥的累积表观利用率、农学效率和偏生产力,MNPK仅显著提高了氮肥的累积表观利用率、农学效率和偏生产力。除了PK和MNPK外的其它处理,土壤钾素均呈亏缺状态,以NP亏缺最严重,达到103 kg hm-2a-1。施钾肥对钾肥累积表观利用率、钾肥农学效率以及钾肥偏生产力均没有明显影响。20年后,土壤有机质、全氮、速效磷和速效钾含量均以MNPK最高。旱作塿土合理的施肥措施为氮磷配合或有机肥配合氮肥。     

长期有机无机肥配施对水稻产量及土壤有效养分影响

利用中国农业科学院红壤实验站开始于1982年的水稻长期定位试验,研究长期有机无机肥配施对水稻产量及土壤速效氮、磷、钾含量的动态变化(1982—2011年)特征。29年研究结果表明:长期有机无机肥配施(NPKM)能提高水稻产量,培肥地力。长期施用化肥(NPK)导致水稻产量降低。稻谷产量随着施肥量的增加而增加。在等氮投入情况下,增施化学磷肥的增产效应要高于化学钾肥,且早稻表现尤为明显;长期单施有机肥和单施化肥对稻谷产量的影响没有显著差异。随着施肥时间的延长,各处理水稻产量差异越显著。各施肥处理土壤碱解氮增加速率表现为慢-快-慢三个阶段,有机肥的施用相较单施化肥,能够显著提高土壤碱解氮含量(p0.05)。土壤有效磷的累积主要与化学磷肥的施用有关,各施肥处理土壤有效磷历年平均含量变化趋势为:NPKM、NPM、PKMNPKM、NKM(p0.01);土壤速效钾以有机肥和化学钾肥配施的处理(NPKM、NKM、PKM)增加最快,单施化学肥料的处理(NPK)增加最慢。随着氮、磷施用量的增加,土壤中氮、磷素出现盈余,但NPKM处理相比其他处理能够有效降低盈余量;各处理土壤中钾素均表现为亏缺状态,红壤性水稻田至少每年应补充投入钾素200 kg hm-2才能基本维持土壤钾素平衡。     

磷钾调控对冷浸田水稻产量和养分吸收的影响

【目的】冷浸田是典型低产稻田,其施肥结构和模式与普通稻田应存在差异。本研究拟在普通稻田施肥结构的基础上,通过调整肥料结构,探索冷浸田最佳施肥模式,为提高冷浸田水稻产量提供数据支撑和理论依据。【方法】通过连续3年的定位试验,以水稻产量为目标,通过测定产量构成因素、水稻养分吸收和土壤养分变化,分析冷浸田施肥模式,提出冷浸田最佳施肥用量。【结果】土壤速效磷含量介于2.7~7.3 mg/kg之间,速效钾含量介于20~80 mg/kg,明显低于普通稻田（平均速效磷11.5 mg/kg,平均速效钾95.8 mg/kg）,是冷浸田主要养分障碍因子;施肥可以极大提高水稻产量,其中NPK、NP2K、NPK1、NPK+Zn和NPK2处理的产量比不施肥（CK）或习惯（FP）高20%~30%左右。在氮磷钾基础上增施锌肥、硅肥和钾肥增产达到显著水平,降低磷肥和钾肥用量则有降低产量的趋势。连续三年种植水稻土壤碱解氮、速效钾呈现平稳,速效磷有增加趋势。施肥处理水稻籽粒和茎部钾含量明显高于CK,籽粒氮累积量显著高于CK和习惯施肥处理。相对于NPK、CK、FP处理,增施磷、钾肥,添加微肥可显著提高籽粒氮、磷、钾累积量。【结论】鄂东南低丘区冷浸田速效磷、速效钾含量低,是主要营养限制因子,需要长期加大磷、钾肥施用量;该地区冷浸田适宜施肥量为N 180、P2O5 90~108、K2O 120~144 kg/hm2,同时配施锌肥和硅肥,连年施用可保证冷浸田水稻的稳步增产。     

灌淤土区长期施钾对作物产量与养分及土壤钾素的长期效应研究

通过在宁夏灌淤土区长达14年的连续施钾和小麦秸秆还田试验, 研究钾素投入对作物产量、养分和土壤钾素状况的影响。结果表明：小麦秸秆还田和长期施用钾肥均可不同程度提高小麦和玉米的经济产量, 其中施钾年平均增产小麦244 kg·hm^-2, 玉米397 kg·hm^-2, 处理之间产量表现为氮磷钾肥配合秸秆还田〉施用氮磷钾肥〉氮磷肥配合秸秆还田〉只施用氮磷肥。定位后8-10年施钾肥开始显著有效, 玉米显效时间早于小麦;秸秆还田和钾肥的投入均可提高籽粒和秸秆的钾素吸收量, 秸秆含钾丰富, 籽粒钾含量仅占植株钾总量的13%-17%;施用钾肥可提高作物籽粒大中微量元素含量而降低秸秆中、微量元素含量, 促进籽粒对大部分元素的吸收;长期不施钾肥处理（NP和NP＋St）0-20 cm土层土壤速效钾和缓效钾含量较定位开始时下降; 所有处理土壤全钾含量均表现下降, 下降幅度为0.8-1.2 g·kg-1。     

长期施用化肥和稻草对红壤性水稻土钾素固定的影响

研究了长期定位施肥试验中连续27年(1981-2007年)施用化肥和稻草对红壤水稻土钾素固定及其影响因素的影响。本试验选择了其中5个处理:CK(不施肥)、NP(施氮、磷肥)、NPK(施氮、磷、钾肥)、NP+RS(施氮、磷肥和稻草)和NPK+RS(施氮、磷、钾肥和稻草)。结果表明,在加入外源钾浓度0.4～4.0g/L的范围内,土壤固钾量均随着加入外源钾浓度的增加而增大。5个施肥处理的固钾能力存在差异,与试前土壤相比,长期不施钾(CK和NP)处理土壤的固钾能力增强,长期施钾肥或稻草(NPK、NP+RS和NPK+RS)处理土壤的固钾能力降低,长期施用钾肥和稻草是影响土壤固钾能力的重要原因,施钾量高的处理土壤的固钾能力低。在高强度稻-稻种植条件下,水稻作物从土壤中带走大量的钾。长期不施用钾肥,会导致土壤钾素的严重耗竭,此后施入的钾则会被土壤固定,导致钾肥对当季作物的有效性降低。当土壤中钾含量相对较高时,施入钾肥则不易被吸附固定。如果施入的钾不能及时被作物吸收利用,则极易被淋失。长期施用钾肥和稻草后,土壤速效钾和缓效钾含量增加,土壤的固钾能力降低;另外,长期施用钾肥和稻草引起土壤K+饱和度的增加,也使土壤的固钾能力降低。     

长期施钾和秸秆还田对河北潮土区作物产量和土壤钾素状况的影响

以河北冲积平原典型潮土区小麦-玉米轮作长期定位试验（19922009年）为基础,分析了长期施用钾肥和秸秆还田对作物产量、 土壤钾含量及钾素平衡的影响。研究结果表明,不同处理下小麦、 玉米17年平均产量均表现为NPKStNPKNPStNP,其中,小麦、 玉米NPKSt处理比NP处理分别增产9.37%和19.23%,NPK处理比NP处理分别增产6.32%和18.25%; NPSt处理比NP处理分别增产2.76%和9.60%; 长期定位施肥下,各处理耕层土壤（020 cm）各施钾肥的处理其速效钾、 缓效钾含量均与NP处理差异极显著,且表现为NPKStNPKNPStNP; 各处理下层土壤（2040 cm）的速效钾、 缓效钾含量差异均不显著,从钾素平衡看,NP处理钾素表观年盈亏量为-170.0 kg/hm2,土壤钾严重亏缺; NPSt处理实际平衡盈亏率-58.4%,表观年盈亏量为-150.6 kg/hm2; NPK处理钾素表观年盈亏量-3.1 kg/hm2,盈亏率-1.0%,基本接近平衡; NPKSt处理实际平衡盈亏率36.8%,表观年盈亏量为111.4 kg/hm2。试验结果说明,在潮土区,在施氮、 磷肥的基础上,配施钾肥或秸秆还田不仅能持续提高小麦、 玉米的高产、 稳产生产能力, 而且对保持农田土壤钾素平衡、 有效改善耕层土壤钾素状况、 提高土壤肥力有重要的作用。     

Role of selenium in mitigation of cadmium toxicity in pepper grown in hydroponic condition

Selenium (Se) is an essential element for humans but is not considered as essential for plants. However, its beneficial role in improving plant growth and stress tolerances is well established. In order to study the role of Se in cadmium (Cd) toxicity in pepper (Capsicum frutescens cv. Suryankhi Cluster), this experiment was carried out in greenhouse conditions. Treatments comprised Cd [0, 0.25, and 0.5 mM cadmium chloride (CdCl₂)] and Se [0, 3, and 7 µM sodium selenite (Na₂SeO₃)] with three replications. The result showed that Cd decreased chlorophyll a, chlorophyll b, and carotenoids, whereas Se supplementation diminished Cd toxicity on photosynthetic pigment. Selenium at 7 µM significantly increased the leaf area in the plants grown at 0.25 mM Cd. The application of Se at 3 µM with 0.25 mM Cd and Se at 3 µM and Se at 7 µM with 0.5 mM Cd increased the activity of catalase (CAT). Selenium at 7 µM decreased the proline content of pepper leaves exposed to Cd at 0.5 mM (30%). Selenium significantly enhanced the antioxidant activity of leaves, which was diminished by Cd toxicity. In general, Se has a beneficial effect on plant growth and is an antioxidant enzyme of pepper cv. Suryankhi Cluster under Cd stress and non-stress conditions.     

红壤区不同肥力水稻土根际硝化作用特征

通过根际培养箱(三室)——速冻切片技术研究了红壤地区高、低两种肥力下水稻苗期根表、根际和土体土壤矿质态氮含量和硝化强度,以及水稻生长、氮素积累的差异。结果表明,肥力水平对水稻根表和根际土壤铵态氮(NH4+-N)含量无显著影响,但高肥力显著提高土体土壤NH4+-N含量,以及根表、根际和土体土壤硝态氮(NO3--N)含量及硝化强度。两种肥力水稻土硝化强度最大值均出现在距根表2 mm处,分别为0.20和0.31μmol kg-1 h-1。土体土壤硝化强度随距根表距离增加而降低,低肥力土壤在距根表10～40mm处时硝化强度接近本底值,而高肥力土壤在距根表20～40 mm处时接近本底值。与不种水稻的CK相比,种植水稻显著提高根际土壤硝化强度。高肥力能显著改善水稻生长,增加植株氮素积累量,尤其显著促进根系生长及通气组织发育。由于红壤稻田肥力水平的差异造成水稻根际硝化强度以及水稻吸收NO3-的差异,导致高肥条件下水稻显示出更强的生长势和氮素吸收利用能力。因此,合理提高红壤稻田肥力水平对改善红壤区水稻根际土壤硝化作用及水稻氮素营养具有重要意义。     

Accumulation of copper in soils and leaves of tomato plants in greenhouses in Turkey

Copper (Cu)‐containing fertilizers, fungicides, and bactericides has been used extensively in the greenhouses in the Antalya province in recent decades with the use of such materials tending to increase year by year. The level of DTPA‐extractable Cu was determined in 210 soils (0–20 and 20–40 cm depth) and 105 leaf samples obtained from 105 tomato greenhouses. The DTPA‐extractable Cu contents of the soils taken from the 0–20 depth ranged between 0.76 and 88.03 mg kg^‐1 (mean 7.79 mg kg^‐1). The percentage of soils containing DTPA‐extractable Cu greater than the critical toxicity level (20 mg kg^‐1) was 8.1. The Cu content of tomato leaf samples ranged between 2.4 and 1,490 mg kg^‐1 (mean 166.5 mg kg^‐1). The Cu concentration in leaf samples was very high due to the intensive use of foliar applied Cu‐containing chemicals. As a consequence, 24.8% of the leaf tomato samples analyzed contained over 200 mg Cu kg^‐1, the maximum accepted tolerance level. Therefore, it may be necessary to reduce the use of Cu‐containing pesticides and fertilizers, or at least reduce the amount of Cu‐containing fertilizers being used in those greenhouses where Cu‐containing pesticides have been or are being used.     

自由基在鸡肾病发生中的作用研究

肾病即急性肾小管坏死.鸡肾病在养鸡生产中极为常见,尤其近年来,鸡肾病的发生日益增多,由于其发生机制不清楚,临床治疗鸡肾病的依据不足,致使治疗效果不佳,为此,提出本研究以探讨鸡肾病的发生机制,为本病防治提供理论依据.     

Occurrence of Sexual Hormones in Sediments of Mangrove in Brazil

The presence of sexual hormones (female estrogens) was assessed in sediments of a mangrove located in the urban region of southern Brazil. The estrogens are involved in human sexual reproduction. They act as the chemical messengers, and they are classified as natural and synthetic. The estrogens inputs in the environment are from treated and untreated sewage. The presence of estrogens in sewage is excretion from the female due to natural production and use of contraceptives (synthetic estrogens). With the indiscriminate release of sewage into the environment, estrogens can be found in rivers, lakes, and even in oceans. In this work, the presence of estrone (E1), 17-??-estradiol (E2), and 17-??-ethynilestradiol (EE2) in eight sedimentary stations in Itacorubi mangrove located on Santa Catarina Island, south Brazil, was investigated. Historically, the Itacorubi mangrove has been impacted by anthropogenic activities because the mangrove is inserted in the urban area of the Florianopolis. The estrogen EE2, used as contraceptive, had the highest concentration in mangrove sediment, 129.75?±?3.89 ng/g. E2 was also found, with its concentration ranging from 0.90?±?0.03 to 39.77?±?1.19 ng/g. Following the mechanism, under aerobic or anaerobic conditions, E2 will first be oxidized to E1, which is further oxidized to unknown metabolites and finally to CO₂ and water (mineralized). EE2 is oxidized to unknown metabolites and also finally mineralized. Theoretically, under anaerobic conditions, EE2 can be reduced to E1 even in environments such as mangrove which is essentially anaerobic.     

Meta-analysis in the Selection of Groups in Varieties of Citrus

Brazil is the largest producer of oranges (Citrus sinensis) in the world. The nutrient management of tree orchards is designed from experiments with a limited number of varieties. This knowledge is transferred to other varieties by diagnosing tissue nutrient composition and tree demand. Compositional data analysis has been first applied to tissue analysis of agricultural crops using centered log ratios with compositional nutrient diagnosis (CND-clr). The isometric log ratio (ilr) transformation is a new approach based on binary nutrient ratios and the principle of orthogonality (CND-ilr). We analyzed eleven nutrients: nitrogen (N), sulfur (S), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), boron (B), copper (Cu), zinc (Zn), manganese (Mn), and iron (Fe) in leaf tissue samples across 108 commercial plots (thirty-one grow Valencia, twenty-two Hamlim, twenty Pera, and thirty-five Natal). Nutrients were partitioned between macro- and micronutrients as well as anionic and cationic species. The effect size of varieties over Valencia was quantified by the mean and standard deviation of ilr values across ilr coordinates. Specific varietal nutrient profiles and ilr norms were defined. The nutrient profile of orange varieties could be classified into homogeneous groups to take advantage of fertilizer trials conducted on varieties of the same group. The Aitchison distance and a perturbation vector could be instrumental for diagnostic purposes and nutrient management.     

Effect of iron-quercetin complex on reduction of nitrite in in vitro and in vivo systems

This study investigated whether reducing agents such as quercetin and iron(II) facilitate formation of nitric oxide (NO) gas from orally ingested nitrite in an vivo study. When 3 mg/kg Na (15)NO2 was orally administered to rats with or without iron(II) or quercetin, Hb (15)NO, which is indicative of systemic (15)NO, was detected in the blood, with the maximum blood concentration of Hb (15)NO at 15 min after nitrite or nitrite plus quercetin treatment, whereas after administration of nitrite plus iron(II) or nitrite plus iron(II) and quercetin, the time was shortened to 10 min. Interestingly, iron(II), quercetin, or iron(II) plus quercetin did not affect the total amount of Hb (15)NO generated from orally administered Na (15)NO2. However, the systemic nitrite concentration was significantly decreased in the presence of iron(II) or iron(II) plus quercetin. These results may indicate that iron(II) is critical to the generation of NO gas from nitrite, whereas quercetin contributed little under the in vivo experimental conditions.     

旱地果园水肥管理模式研究进展

分析了旱地果园水肥管理模式的研究进展和存在问题,指出水肥胁迫尤其是水钾已成为旱区果树高产稳产优质化的主要限制因子。在系统探讨旱地果园水分管理、养分管理、综合管理以及果树叶营养综合诊断施肥标准的基础上,提出了旱地果园的高效水肥管理模式。     

Role of aldehyde oxidase in the hepatic in vitro metabolism of 3-methylindole in pigs

The metabolism of 3-hydroxy-3-methylindolenine (HMI), a recently discovered metabolite of 3-methylindole (3MI, skatole) produced by porcine liver microsomes, was investigated in vitro using porcine liver cytosol. HMI was rapidly metabolized to a single product, 3-hydroxy-3-methyloxindole (HMOI), by porcine cytosol. By the use of the selective inhibitors menadione and quinacrine, it was shown that the enzyme responsible for the oxidation of HMI into HMOI was aldehyde oxidase (AO; aldehyde:oxygen oxidoreductase, EC 1.2.3.1). The activity of AO in the conversion of HMI to HMOI was measured in a population of pigs (n = 30) with a wide range of 3MI levels in back fat (0.07-0.30 mg/kg). AO activity was found to be negatively correlated (r = -0.70; P < 0.001) with the level of 3MI in fat. The results of the present study suggest that AO plays an important role in the metabolism of 3MI in the pig and that its catalytic activity is related to an adequate 3MI clearance.     

植物CBL基因家族的研究进展

细胞中游离的Ca2+是植物细胞中普遍存在的第二信使,类钙调神经素B亚基蛋白CBL作为一种特殊的钙感受器在植物生长发育和逆境胁迫响应过程中发挥重要作用.本文主要对植物CBL家族的起源与进化、蛋白结构、亚细胞定位以及CBL的功能进行综述,并对今后钙感受器CBL家族的研究重点和发展方向进行了展望.