铝胁迫下磷对水稻苗期生长的影响及水稻耐铝性与磷效率的关系

选用两个耐铝性差异较大的水稻品种武运粳7号(耐铝品种)和扬稻6号(铝敏感品种)作为实验材料,利用水培铝-磷交替处理试验研究了磷对水稻铝胁迫下苗期生长的影响及水稻耐铝性与磷效率之间的关系。结果表明,从水稻铝-磷交替处理下的生物量和根系形态等指标来看,P缓解了Al对两个水稻品种的毒害作用,且对敏感品种扬稻6号的缓解作用更加明显。铝-磷交替处理下,武运粳7号的体内磷含量显著高于扬稻6号的,而根部铝含量显著低于扬稻6号,因此武运粳7号体内的P/Al显著高于扬稻6号;同时,虽然两个品种间根表及根自由空间中Al浓度没有差别,而武运粳7号根表及根自由空间中P浓度显著高于扬稻6号,表现出耐铝品种更强的质外体解铝毒能力,这可能与武运粳7号较强的磷吸收效率有关。此外,与耐铝品种武运粳7号相比,铝敏感品种扬稻6号虽然磷吸收效率低,但利用效率高,即两个水稻品种的耐铝性与水稻的磷吸收效率和利用效率不一致,这为酸性土壤水稻育种提供了理论基础,即选育适应酸性土壤的水稻基因型不仅要关注其耐铝性还要关注其磷利用效率。     

大豆种质耐低磷与耐铝毒部分指标及其相互关系的研究

通过溶液培养试验 ,对来源于华南酸性土壤的 5个大豆品种的地上部及根部耐低磷及耐酸铝部分指标进行了系统的研究。研究结果表明 ,大豆在磷效率和耐铝毒特性方面存在着一定的差异 ,大豆品种 6 2号、乐昌及广州大粒具有较高的磷效率和耐铝毒特性。在低磷及铝毒胁迫下它们具有较高的生物量、磷含量、叶面积、叶绿素含量、总酸分泌量以及主根长。低磷和铝毒胁迫严重影响地上部磷的吸收 ,高磷能缓解铝毒。铝浓度不同处理大豆的地上部无显著差异 ;但在铝毒胁迫下 ,根部的铝浓度比地上部高 5～ 6倍 ,且品种间存在显著差异。本研究初步确定了大豆耐低磷与耐铝毒的相关关系     

不同pH低磷土壤上水稻磷营养特性研究

通过盆栽试验,研究了不同磷效率水稻在不同pH土壤和不同磷处理水平下的磷营养特性。结果表明,低磷条件下,分蘖期耐低磷基因型850、1574和1079的磷吸收效率最高,孕穗期3个耐低磷品种磷吸收量和利用效率均较高;到成熟期1574品种的相对磷吸收量最高,达到65%,而相对磷利用效率则以1574和850品种最高。不同pH土壤上,分蘖期,各水稻品种在碱性土壤上磷吸收量和利用效率最低,相对磷吸收量和相对磷利用效率却最高;耐低磷基因型850、1574和1079的磷吸收量高于鄂晚13和敏感基因型99012。在孕穗期,各水稻品种在碱性土壤上的磷吸收量高于其它两种土壤,但相对磷利用效率却最低;耐低磷基因型850和1574在3种土壤上磷吸收量均最高,成熟期各水稻品种磷利用效率则明显高于孕穗期。可见,不同耐低磷基因型在不同pH土壤上表现出的磷营养特性不同,在耐低磷品种的筛选过程中应该考虑土壤pH因素,生产中应根据不同土壤酸度类型选择合适的品种。     

硅对籼稻和粳稻品种磷积累的影响

硅(Si)可以改善磷(P)的营养,但是其内在机制尚不明确。本研究以4个籼稻和3个粳稻品种为研究对象,通过水培试验探究硅对不同水稻品种磷积累的影响。在营养液中添加硅显著降低了籼稻和粳稻品种的地上部磷含量,但几乎不影响根部磷含量。加硅处理不影响所有供试水稻品种的磷吸收及磷从根系向地上部的转移,但提高了磷的利用效率,增加了植株P/Mn和P/Zn比率。磷转运蛋白(PT)基因表达分析表明,在粳稻品种中只有OsPT6被硅下调,而OsPT6在籼稻品种中没有显著性变化;其他PT基因的表达不受硅的影响。由此,硅对不同水稻品种的磷积累有不同的影响,磷利用率的提高可能归因于较高的P/Mn和P/Zn比率,从而导致植物中较高的有效磷。     

不同供磷水平对旱作条件下水稻生长、根系形态和 养分吸收的影响

通过盆栽试验,比较分析了磷素对旱作条件下不同水稻品种苗期生长、根系形态及磷素吸收利用效率的影响。结果表明,施用磷肥促进水稻地上部和根系的生长,低磷胁迫显著增大了植物的根冠比,且品种间差异明显,丛矮2在低磷水平和高磷水平下的根冠比比值为1.982,而黄华占相应的比值为1.096;随供磷浓度的增加,水稻植株含磷量增加而磷素生理利用率降低,在3种磷水平下,3345的磷素吸收效率均高于其他4个品种,磷素生理利用率却低于其他4个品种。根系形态参数与磷素吸收、利用效率的相关性分析表明:根系总长对水稻植株吸磷量影响最大。总之,适当地施用磷肥能更好地协调根系与地上部的关系,促进根系的生长和根系对磷素的吸收。     

不同磷效率油菜根际土壤磷活化机理研究

筛选和培育耐低磷植物是缓解磷矿资源缺乏和提高磷肥利用效率的有效途径之一。本研究在前期材料筛选的基础上,通过根箱试验研究不同磷效率油菜根际土壤磷活化机理,其结果如下:在施磷和不施磷处理条件下,磷高效油菜品种B56（HG）的吸磷量和生物量均高于磷低效品种B10（LG）;两油菜品种根际土壤中的NaHCO3-Pi, NaHCO3-Po和 NaOH-Pi,NaOH-Po四种磷素形态均有显著的亏缺现象;磷高效品种（HG）根际土壤上述四种磷素形态亏缺程度大于磷低效品种（LG）,但Resin-P则出现富集,并且HG的富集程度大于LG,这可能与HG具有较高吸磷能力有关。两油菜品种根际土壤HCl-Pi 和 残渣态磷（Residual-P）没有发生明显的亏缺现象。相比较而言,HG能分泌更多的酸性磷酸酶,该酶活性与NaHCO3-Po含量呈显著的负相关,说明酸性磷酸酶对有机磷矿化起着非常重要的作用。     

不同供磷水平下水稻磷素吸收利用和产量的基因型差异

通过田间试验研究了不同供磷水平对8个水稻品种磷素吸收利用和稻谷产量的影响。研究结果表明,两种供磷水平下,水稻的稻谷产量、磷利用效率和各生育期地上部磷积累都存在显著的基因型差异。低磷胁迫显著降低水稻的稻谷产量和各生育期地上部磷积累,显著提高水稻的磷利用效率。相关性分析表明,低磷胁迫下水稻稻谷产量与水稻磷利用效率、生育前期(秧苗期+分蘖期)地上部磷积累以及生育中期(抽穗期)地上部磷积累呈显著正相关(p<0. 05);正常供磷条件下水稻稻谷产量与磷利用效率、生育前期地上部磷积累呈显著正相关(p<0. 05)。因此筛选和培育具有较高磷利用效率和在生育前期具有较强磷素积累特性的水稻基因型可能是缓解南方水稻土磷素严重缺乏的有效途径之一。     

不同年代棉花品种磷效率比较

提高磷肥利用率是当今农田磷养分资源管理面临的重要难题,作物品种改良是提高磷利用率的有效途径之一。为明确新疆不同年代棉花品种(系)磷效率的差异,采用单因素随机区组设计,通过大田试验,以新疆1950s—2013年不同年代的22个棉花品种(系)为材料,研究了不同年代培育的棉花品种(系)在苗期、蕾期、花铃期和吐絮期的磷吸收、利用效率特征,并采用模糊数学隶属函数和聚类分析方法,对不同棉花品种(系)磷效率的高低进行了综合评价。结果表明:不同棉花品种(系)磷吸收效率、利用效率、磷由茎叶向籽粒的转移效率(再转移效率)存在差异。吐絮期磷吸收效率为164.26～395.75 mg·株~(-1),变异系数为3.3%;磷利用效率为0.25～0.40 g·mg~(-1),变异系数为67.7%;磷再转移效率为16.0%～58.1%,变异系数为11.1%。以每20年为一个时期,划分了3个品种更替阶段,棉花磷利用效率在3个阶段没有发生明显改变,始终维持在0.30～0.32 g·mg~(-1);磷吸收效率由225.5 mg·株~(-1)增加到286.3 mg·株~(-1),提高了27.0%;磷由根系向地上部的转移效率(转移效率)没有发生明显改变,始终维持在93.9%～94.9%;磷再转移效率由26.8%增加到38.3%;磷经济利用效率由20.6 mg·mg~(-1)增加到30.4 mg·mg~(-1)。相关性分析表明:不同棉花品种(系)生物量与磷吸收效率呈显著正相关,而与磷利用效率无显著的相关性;皮棉产量与磷吸收效率、转移效率、再转移效率、经济利用效率呈极显著正相关,而与磷利用效率相关性不明显。对22个棉花品种(系)吐絮期生物量、皮棉产量、磷吸收利用效率及转移效率和经济利用效率等性状指标的综合评价和聚类分析,将22个品种(系)分为磷高效型、中效型、低效型和极低效型4组,‘新陆早50号’‘新陆早57号’‘军棉1号’为磷高效型品种。与磷低效组相比,磷高效组的品种具有较高的生物量和产量。以上结果表明,新疆棉花高产育种过程提高了磷素吸收、再转移和经济利用效率。     

水稻和两种野生植物对酸性硫酸盐土耐性及矿质元素吸收

酸性硫酸盐土是发育于还原性硫化物成土母质的一种恶劣土壤类型,虽然其分布区气候条件十分有利于水稻生长,但由于土壤条件限制,水稻生产潜力难以发挥。为筛选出适应该土壤的水稻品种,在酸性硫酸盐土(pH 3.69)上种植了240个水稻品种,但是所有水稻品种均未获得经济产量,却发现两种野生植物在该土壤中生长茂盛。经形态和分子鉴定,两种野生植物为野荸荠(Eleocharis kuroguwai)和五棱飘拂草(Fimbristylis quinquangularis)。进一步分析了水稻和两种野生植物的根际和非根际土壤性质、根部和地上部矿质元素含量。结果表明,根际土壤pH、交换性铝和有效锰含量不能解释水稻和两种野生植物耐性差异,锰毒和缺氮亦不是酸性硫酸盐土限制植物生长的关键因子,而植物地上部矿质元素含量与植物耐性差异相关。铝毒、铁毒和较低的养分(磷、钾、钙、镁)吸收是酸性硫酸盐土中水稻生长不良的主要因子。相反,两种野生植物对铝毒和铁毒的耐性较强,对养分吸收能力较强,所以生长良好。     

石灰性土壤施用不同磷肥对玉米苗期生长和土壤无机磷组分的影响

研究旨在探讨不同磷肥品种对玉米生长发育和土壤无机磷组分的影响,以期为磷肥高效利用提供参考。采用盆栽试验,设置6个处理:磷酸一铵(MAP)、过磷酸钙(SSP)、聚磷酸铵(APP)、氮磷复合肥硝酸磷肥(NiP)、硫酸铵+过磷酸钙混施(SA+P),试验60天后测定了玉米的生物学指标和玉米植株磷素含量,同时测定了土壤有效磷与土壤无机磷组分含量状况。结果表明:玉米生物学性状、磷素累积量和磷肥利用效率均表现出APPMAP、NiPSSP、SA+PCK的趋势。相较于对照,APP、MAP、NiP处理显著提高玉米植株的株高、叶面积、地上部和根系干重,株高增幅在23.3～35.1 cm,叶面积增加57.1～89.0 cm~2,地上部和根系干重分别增加265%～420%和171%～218%。APP处理植株磷素累积量达到49.02 mg/盆,磷肥利用效率达到36.75%,显著高于NiP和MAP的21.43%和19.42%。相较于对照,APP和MAP处理的Ca_2—P、Ca_8—P和Fe—P呈显著性增加,上述3种无机磷组分含量与玉米植株磷累积量呈极显著正相关关系(p0.001)。北方石灰性土壤聚磷酸铵(APP)是一种表现良好的磷肥类型,这对减磷增效背景下的粮食安全和磷肥高效利用有重要应用价值。     

Phosphorus Nutrition of Lowland Rice in Tropical Lowland Soil

Rice is a main food crop for about half of the world's population, and phosphorus (P) is the main limiting nutrient in rice production in tropical lowlands. A greenhouse experiment was conducted to evaluate P requirements of lowland rice grown on a lowland soil (Inceptisol). Dry matter, grain yield, and yield-attributing characteristics were significantly (P < 0.01) influenced by P fertilization. Based on quadratic response, maximum shoot dry weight and grain yield were obtained with the application of 190 mg P kg^?1 of soil. Maximum panicle, tiller number, and plant height were obtained with the application of 177 192, and 175 mg P kg^?1 of soil, respectively. Mehlich 1–extractable P for maximum grain yield was 15.6 mg kg^?1 of soil. Variability in grain yield with plant growth and yield parameters was in the order of tiller > shoot dry weight > panicle number > spikelet sterility > plant height > grain harvest index > panicle length > weight of 1000 grains. Phosphorus uptake in shoot and concentration and uptake in grain significantly (P < 0.01) increased grain yield. However, variability in grain yield was greater with concentration and uptake of P in the grain. Similarly, P harvest index was also significantly associated with grain yield. Agronomic P-use efficiency, apparent P-recovery efficiency, and P-utilization efficiency decreased quadratically with increasing P rates, whereas physiological P-use efficiency increased quadratically and agrophysiological P-use efficiency decreased linearly with increasing P rates. Agrophysiological and utilization P-use efficiencies had significant positive correlation with grain yield.     

Differences Among Rice Cultivars in their Adaptation to Low Ionic Strength Solution with Toxic Level of Aluminum that Mimics Tropical Acid Soil Conditions

Nutrient deficiencies are often an additional growth-limiting factor in tropical acid soils. Considering the potential interactions between Al stress and low-nutrient stress, differences among rice cultivars for Al tolerance, low-nutrient tolerance, and combined stress tolerance were investigated. The main objective of this study was to identify the predominant growth-limiting factor in tropical acid soils. Tolerance to low nutrient stress and combined stress did not show any relationship with aluminum (Al) tolerance indicating that these stress factors act independently. Al-tolerant cv. Rikuu-132 was tolerant to combined stress. Conversely, highly Al-sensitive cv. BR34 was most tolerant to combined and low nutrient stress. Combined stress tolerance of shoot was positively correlated with calcium (Ca) content of shoot. The results indicate that Al tolerance alone is not adequate for superior performance on most acid soils. Tolerance to combined stress factors would be needed to improve productivity of rice on low fertility acid soils.     

不同生态稻作区氮肥增产效应及其利用率

在4个典型生态粳稻区,以广适性的5个品种为材料,研究氮肥对不同稻区产量影响,并分析氮肥利用率及其不同稻区施氮效果的差异。结果表明:氮肥施用显著提高不同稻区产量,施氮增产率及其贡献率大小顺序为温暖粳稻区寒冷粳稻区籼粳交错区冷凉粳稻区,寒冷粳稻区产量提高主要是有效穗数和千粒重的增加,而其它3个稻区产量的提高主要是有效穗数和每穗粒数的增加所致。生产等量的稻谷氮肥需要量的大小顺序为寒冷粳稻区冷凉粳稻区籼粳交错区温暖粳稻区。不同稻区对氮肥的利用效率存在差异,氮肥农学利用率大小顺序为温暖粳稻区籼粳交错区冷凉粳稻区寒冷粳稻区,氮肥吸收利用率大小顺序为籼粳交错区温暖粳稻区冷凉粳稻区寒冷粳稻区,4个稻作区的土壤氮素贡献率在61.10%~66.09%之间,说明各稻作区吸收的氮肥主要来自土壤,通过培肥地力,维持较高的地力水平对稻谷的超高产、高效具有重要意义。     

Comparison of Phosphorus Use Efficiency Among Various Winter Wheat Accessions Grown in Acid and Calcareous Soils

The objective was to differentiate and estimate phosphorus (P)-uptake and P-utilization efficiency among several winter wheat accessions. Twenty-two accessions were characterized for their ability to uptake and utilize P in two low P soils (acid and calcareous). Plants were grown for 28 days in two different sized pots and analyzed for biomass and total P concentration. Total P uptake and biomass was greater in the large pot and calcareous soil. However, there were no significant correlations between the small and large pot for biomass, P uptake, and P concentration, indicating that rooting conditions were different for the two pots, which allowed differentiation between P-utilization and P-uptake efficiency. Possession of ALTM1 gene for malate excretion did not appear to be related to P-uptake efficiency. Several accessions were found to be either P-uptake or P-utilization efficient in both soils. Phosphorus use efficiency mechanisms may contribute to acid soil tolerance.     

Variation in Phosphorus Uptake and Use Efficiencies Between Pigeonpea Genotypes and Cowpea

ABSTRACT

Low bioavailability of soil phosphorus (P) often limits N₂ (nitrogen) fixation and crop production in large parts of the tropics. The efficiency of P acquisition and P use by 21 genotypes of pigeonpea [Cajanus cajan (L.) Millsp.] were studied in a pot experiment using two cowpea [Vigna unguiculata (L.) Waip.] genotypes as controls. The short-duration genotypes produced more dry matter, accumulated more P, and produced more dry matter per unit of absorbed P than the medium-and long-duration genotypes. Dry-matter production correlated positively with the P uptake (r² = 0.72) and P-use efficiency (r² = 0.86). The P uptake correlated positively with the P-use efficiency (r² = 0.36); whereas the P-use efficiency correlated negatively with the P-uptake efficiency (r² = 0.50). Root surface did not determine P acquisition of pigeonpea, which absorbed 1.6 mg P cm^?3 from 33 cm³ soil compared with 0.17 mg P cm^?3 from 387 cm³ soil for cowpea at 66 d.     

Comparison of the physicochemical properties and ultrastructure of japonica and indica rice grains

The physicochemical properties and ultrastructures of japonica vs indica rice varieties and waxy vs nonwaxy rice varieties were compared. The viscogram values of the indica varieties were significantly higher than those of the japonica varieties. The gelatinization temperatures, breakdown, and setback were significantly lower for waxy than for nonwaxy rice varieties. Japonica rice exhibited lower hardness but higher adhesiveness than indica rice. The air space between individual starch granules was larger for waxy than for nonwaxy rice. The starch granules were compact in japonica rice, while the compound starch granules of indica rice were much smaller than those of japonica rice and were scattered widely in the endosperm. The protein bodies in japonica rice were concentrated near the cell wall, whereas those in indica rice were scattered around amyloplasts. These results suggest that the ultrastructure of rice affects the texture of the cooked product.