新疆石河子地区玉米产量及氮素平衡的施氮量阈值研究

【目的】合理施用氮肥不仅会提高肥料利用率,还会降低氮素面源污染的风险。通过2年田间肥料定位试验,研究北疆灰漠土区不同氮肥用量下,土壤无机氮积累量、 氮素平衡和玉米产量间的相互关系,为氮肥合理施用提供依据。【方法】研究采用肥料田间定位试验,小区试验于2011-2012年开展,设计6个氮肥（N）用量水平: 0、 225、 300、 375、 450、 600 kg/hm2,分别以N0、 N225、 N300、 N375、 N450、 N600表示,其中300 kg/hm2为当地玉米农田氮肥推荐用量,磷肥（P2O5）施用量为75 kg/hm2,钾肥（K2O）施用量为37.5 kg/hm2。【结果】 1）施用氮肥增加了土壤硝态氮和铵态氮残留量,硝态氮主要残留于060 cm土层,铵态氮主要分布在020 cm土层深度。2011年试验中,土壤无机氮残留量随氮肥用量增加而显著增加,与对照相比,施氮处理无机氮残留量增幅为12%~102%,与施氮量呈指数增长关系。2012年氮肥用量对土壤无机氮残留量的影响与2011年相似。2）施氮量 225 kg/hm2时,0100 cm土层深度土壤无机氮积累量降低,表现为负积累效应,N0和N225处理下2012年土壤无机氮积累量分别较2011年降低165%和170%; 施氮量高于 300 kg/hm2时,土壤无机氮积累量显著增加,表现为富集现象,其中,N375、 N450和N600处理下2012年土壤无机氮积累量分别较2011年增加17%、 388%、 170%。土壤无机氮积累量与施氮量显著呈二次抛物线关系,2011年回归方程为y=0.0001x2 + 0.1013x－22.537（R2 = 0.9288）,无机氮无积累时施氮量为187 kg/hm2; 2012年为 y = 0.0003x2 + 0.1417x - 52.78（R2 = 0.9583）,无机氮无积累时施氮量为245 kg/hm2。土壤氮素表观损失量和氮素盈余量的增加幅度随氮肥用量增加而显著加大。3）氮肥投入可提高玉米产量,产量与施氮量呈显著的二次抛物线或线性加平台的关系,施氮量高于300 kg/hm2时,玉米产量与最高产量差异不显著; 产量与无机氮积累量呈二次抛物线形关系,当土壤无机氮达到平衡时,玉米产量显著低于最高产量,当玉米产量达到最大时,土壤无机氮有一定积累。氮肥利用率则随氮肥用量增加呈指数关系显著降低。施氮量270 kg/hm2为产量与氮肥利用率的交点,施氮量340 kg/hm2 是土壤无机氮残留量与氮肥利用率的交点。【结论】利用产量效应、 环境效应与肥料效应函数的交点确定氮肥投入阈值,是较为优化的方法。合理的氮肥投入不仅能获得玉米高产,降低氮素面源污染风险,还能获得较高的氮肥利用率。因此,施氮量260340 kg/hm2为本研究区玉米高产与环境友好的氮肥投入阈值。     

氮肥管理对小麦产量和氮肥利用效率的影响

氮肥过量施用造成环境污染和生产成本增加等问题已成为限制我国农业可持续生产的主要因素。为实现氮素化肥的高效利用,连续2年在山东省农业科学院作物研究所试验地同一地块利用氮肥梯度带,进行了基于小麦叶片SPAD值的氮素实时管理。结果表明,在基施区随着施肥量的增加,小麦籽粒产量增加,二者呈线性关系,但施氮量207kg/hm2(N207)和276kg/hm2(N276)处理间差异不显著;追施区小麦的2个生长季籽粒产量均以不施基肥拔节期追氮207kg/hm2(N0+207)处理最高,分别为7649kg/hm2和7522kg/hm2,基施氮肥207kg/hm2拔节期不追肥(N207+0)处理最低,仅为7318kg/hm2和7388kg/hm2,差异达显著水平(P<0.05)。基施区各氮肥处理的氮肥表观利用率(RE)和氮肥偏生产力(PFP)均存在显著性差异,均是随着基施量的增加而显著降低。追施区N0+207、N69+138、N138+693个处理的PFP、AE和RE均高于或显著高于N207和N276 2个处理。因此在确定总施氮量的条件下实行基、追肥分施能够显著提高小麦的氮肥表观利用率、氮肥农学效率和氮肥偏生产力。     

减氮条件下砂壤质潮土区小麦–玉米轮作体系氨挥发特征及排放系数

  【目的】  氮肥施用量影响农田氨挥发量和氮肥利用效率。研究减量施氮后土壤的氨挥发特征及排放系数,为科学评估化肥减施对环境的影响提供依据。  【方法】  选取华北砂壤质潮土农田,进行小麦–玉米轮作周年土壤氨挥发监测研究。试验包括不施氮磷钾化肥 (CK)、常规施氮肥 (N)、优化施肥 (OPT)、减量优化 (LOPT) 和优化加有机肥 (mOPT) 5个施肥处理。小麦季常规、优化和减量优化处理施氮量分别为315、225和135 kg/hm2,玉米季分别为330、240和150 kg/hm2。试验采用密闭海绵法,在小麦、玉米基肥和追肥后,定期取样测定不同处理的氨挥发量,并计算氨排放系数。  【结果】  不同施氮量下,供试农田玉米季土壤氨挥发总量在12.8～20.4 kg/hm2,占总施氮量的5.9%～8.5%;小麦季氨挥发总量在6.8～12.0 kg/hm2,占总施氮量的3.3%～5.0%,玉米季氨总挥发量明显高于小麦季。4个施氮处理相比,N和LOPT处理的氨排放系数较高,小麦季分别为3.8%和5.0%,玉米季分别为6.2%和8.5%,而OPT和mOPT处理的氨排放系数相对较低,小麦季分别为3.6%和3.3%,玉米季均为5.9%。除此之外,OPT和mOPT处理小麦和玉米产量显著高于N和LOPT处理(P<0.05),说明过量或过少施氮不利于砂壤质潮土作物产量的提高,适当添加有机肥不仅增产还可以降低氨挥发量。对不同施氮量进行拟合,发现潮土小麦和玉米季常规施氮量处理的氨挥发量在施肥后均呈极显著指数增加趋势 (P<0.01)。  【结论】  华北砂壤质潮土区小麦–玉米轮作体系中,玉米追肥期的氨挥发量高于基肥期,小麦基肥期的氨挥发量高于追肥期,玉米季的总氨挥发量高于小麦季。优化氮肥施用不论是否配合有机肥,均可显著降低小麦季和玉米季的氨挥发量和氨排放系数,提高两季作物的产量,而过量减施氮肥虽然减少了氨挥发量,但大大增加了氨排放系数。     

推荐施肥量下控释肥替代普通尿素提高高粱产量和氮肥生产力

【目的】高粱生产中氮肥施用不合理,氮肥利用效率低,研究适宜重庆地区高粱种植的控释氮肥减施比例,在减少氮肥用量的同时,确保高粱稳产高产,提高肥料利用效率。【方法】田间定位试验于2021、2022年在重庆永川进行,供试高粱品种为晋渝糯3号和金糯粱1号。设置6个处理,分别为不施氮肥(CK);习惯尿素施肥量(U,N 180 kg/hm2);尿素推荐施用量(U1,减N 20%,N 144 kg/hm2);控释氮肥减施氮量20%(C1,N144 kg/hm2);控释氮肥减施氮量30%(C2,N 126 kg/hm2);控释氮肥减施氮量40%(C3,N 108 kg/hm2)。在高粱开花期和成熟期,调查干物质积累量和转运量,成熟期调查植株和籽粒氮素含量、生物量、产量及产量构成因素。【结果】开花期和成熟期高粱干物质积累量均以C1和U1处理最高,C1处理成熟期干物质积累量又显著高于U1。C1处理叶片花前干物质转运量显著高于U。花后干物质积累量C1处理显著高于其他处理,U1和C2处理显著高于U处理。C1处理晋渝糯3号和金糯粱1号花后干物质积累量对籽粒的贡献率较U分别显著提高了11.54%和12.41%。C...     

不同氮水平下黄瓜-番茄日光温室栽培土壤N_2O排放特征

为探讨日光温室黄瓜—番茄种植体系内N2O排放动态变化及其对不同氮水平的响应规律,采用密闭静态箱法,研究了常规氮量(黄瓜季1 200 kg/hm2,番茄季900 kg/hm2)、比常规氮量减25%(黄瓜季900 kg/hm2,番茄季675 kg/hm2)、减50%(黄瓜季600 kg/hm2,番茄季450 kg/hm2)以及不施氮对日光温室土壤N2O排放的影响。结果表明,温度是影响日光温室土壤N2O排放强度的重要因素,4-10月(平均气温为27.4℃)的N2O排放通量最高达818.4μg/(m2·h);而2-3月(平均气温15.1℃)以及11-12月(平均气温14.7℃)期间的N2O排放通量最高仅为464.5μg/(m2·h),比4-10月的N2O排放峰值降低了43.2%。N2O排放峰值在氮肥追施后5 d内出现,N2O排放量集中在氮肥施用后7 d内,可占整个监测期(271 d)排放量的64.7%~67.8%。施氮因增加了土壤硝态氮含量而引起N2O排放爆发式增长,0~10 cm土壤硝态氮含量与N2O排放量呈指数函数关系(P0.01)。日光温室黄瓜—番茄种植体系内的N2O排放量为0.99~9.92 kg/hm2,其中75.6%~90.0%由施氮造成。与常规氮用量相比,氮减量25%和50%处理的N2O排放量分别降低了40.4%和59.3%,总产量却增加4.9%和7.4%。综上所述,合理减少氮用量不仅可显著降低日光温室土壤N2O排放,而且不会引起产量的降低。该研究为日光温室蔬菜生产构建科学合理的施氮技术及估算中国设施农田温室气体排放量提供参考。     

高密度直播油菜高产优质和氮肥高效的适宜氮肥施用模式

【目的】探讨高密度直播油菜(36×104plant/hm2)的合理氮肥管理措施。【方法】2011-2012年油菜季选用甘蓝型油菜品种德油6号,在成都平原稻-油轮作区开展了氮肥施用量、施氮方式对油菜产量、品质和氮肥利用率影响的大田试验。氮肥施用量试验在相同施氮方式(底肥+1次苗期追肥)下设置5个施氮水平(N 0、90、180、270和360 kg/hm2);施氮方式试验在同等施氮量(N 225 kg/hm2)条件下,设置3种施氮方式(一次性底施、底肥+1次苗期追肥、底肥+2次苗期追肥)。【结果】直播油菜农艺性状都随施氮量增多而呈增加趋势,而施氮方式对株高、一次分枝数和单株角果数无显著影响。增施氮肥可显著提高油菜籽粒产量和菜油产量,含油率则有所下降。随施氮量增加,油菜籽芥酸含量呈上升趋势,而硫甙含量略呈降低趋势。施氮方式对油菜籽含油率、芥酸和硫甙含量均无显著影响。油菜产量和氮肥贡献率(NCR)都随施氮量(90 270 kg/hm2)加大而提高,当施氮量继续增加(360kg/hm2)时却出现明显下降。但180与270 kg/hm2两施氮量处理间的油菜产量、氮肥贡献率(NCR)和氮肥表观利用率(REN)均无显著差异。随施氮量增加,氮肥农学利用率(AEN)、氮肥偏生产力(PFPN)和氮肥表观利用率(REN)表现出明显降低趋势。当施氮量≥270 kg/hm2时,氮肥农学利用率(AEN)、氮肥偏生产力(PFPN)和氮肥表观利用率(REN)均显著低于90 kg/hm2处理。同等施氮量下,氮肥分期施用可以提高直播油菜籽粒产量和菜油产量,并以底追两次施氮相对较高。底追两次施氮方式的氮肥利用率(AEN、PFPN、NCR、REN)均相对高于其他施氮方式。【结论】在本试验中等肥力条件下,高密度直播油菜(36×104plant/hm2)的合理氮肥施用模式为施氮量180kg/hm2和底追两次施氮方式。     

不同施氮量对紫色土大白菜季产量和氨挥发的影响

【目的】 研究紫色土丘陵区水稻–大白菜轮作模式下,大白菜季产量、氨挥发损失通量及影响因素,可为四川省紫色土丘陵区农业面源污染防治提供技术支撑。 【方法】 以大白菜为试材进行了田间试验。结合当地农民的施肥习惯,设定了6个施氮肥水平,施氮量依次为N 0、112.5、150、187.5、225、300 kg/hm2,氮肥均等量分为基肥和追肥,分两次施用。采用密闭室连续通气法对大白菜地进行田间原位氨挥发测定。测定在基肥和追肥施用之后的第1天开始,上午9:00—10:00,下午16:00—17:00进行测定,连续测定14 d(降雨停止测定),直至检测不到氨挥发。成熟期调查大白菜产量和全氮含量。 【结果】 大白菜季施氮总量从0增加至300 kg/hm2时,单季氨挥发损失总量由 2.27 kg/hm2增加至22.72 kg/hm2。基肥和追肥施氮量分别从0增加到150 kg/hm2时,基肥后氨挥发总量的变化范围为1.08 kg/hm2到23.58 kg/hm2,显著高于等量追肥后的氨挥发总量 (0.21～2.83 kg/hm2),这与基肥施用时期温度高于追肥施用时期的温度有关。随施氮量增加,大白菜产量增加,但从N 187.5 kg/hm2增加至300 kg/hm2时,大白菜产量增加不显著;氨挥发总量随施氮量增加而增加,但150 kg/hm2与187.5 kg/hm2处理差异不显著,187.5 kg/hm2与225 kg/hm2、300 kg/hm2处理之间差异显著。 【结论】 大白菜季氨挥发主要集中在施肥之后的两周之内,施肥量和温度是影响大白菜季氨挥发的主要因素。综合考虑产量和单季氨挥发损失总量等因素,施氮肥量为N 187.5 kg/hm2时,大白菜的产量和环境效益最佳。      

过量施氮对旱地土壤碳、氮及供氮能力的影响

【目的】过量施氮会影响土壤有机碳、氮的组成与数量,进而改变土壤供氮能力,但关于西北旱地长期过量施用氮肥后土壤有机碳、氮及土壤供氮能力变化的研究尚缺乏。本文在长期定位试验的基础上,通过分析不同氮肥水平特别是过量施氮条件下土壤硝态氮,有机碳、氮和微生物量碳、氮的变化,探讨长期过量施氮对土壤有机碳、氮及供氮能力的影响。【方法】长期定位试验位于陕西杨凌西北农林科技大学农作一站。在施磷(P2O5)100kg/hm2的基础上,设5个氮水平,施氮量分别为N 0、80、160、240、320 kg/hm2。重复4次,小区面积40 m2,完全随机区组排列。种植冬小麦品种为小堰22。本文选取其中3处理,以不施氮为对照(N0)、施氮量N 160 kg/hm2为正常施氮(N160),施氮量N 320 kg/hm2为过量施氮(N320),分别于2012年6月小麦收获后和10月下季小麦播前采集土壤样品,进行测定分析。【结果】过量施氮导致下季小麦播前0—300 cm各土层硝态氮含量显著增加,平均由对照的2.8 mg/kg增加到15.5 mg/kg;同时,0—60 cm和0—300 cm土层的硝态氮累积量分别由对照的47.2和108.9 kg/hm2增加到76.5和727.7 kg/hm2。过量施氮也增加了夏闲期间0—300 cm土层土壤有机氮矿化量,由对照的72.4 kg/hm2增加到130.7 kg/hm2。但过量施氮未显著增加土壤的有机碳含量,却显著增加了土壤有机氮含量,过量施氮0—20、20—40 cm土层土壤有机碳分别为9.24和5.39 g/kg,有机氮分别为1.05和0.71 g/kg,较对照增加52.2%和54.3%。同样,过量施氮未显著影响0—20、20—40 cm土层土壤微生物量碳含量,其平均含量分别为253和205 mg/kg,却显著提高了0—20、20—40 cm土层土壤微生物量氮含量,由对照的24.1和7.5 mg/kg提高到43.6和16.1 mg/kg。【结论】过量施氮可以显著增加旱地土壤剖面中的硝态氮累积量、夏闲期氮素矿化量、小麦播前土壤氮素供应量和土壤微生物量氮含量,但对土壤有机碳和微生物量碳没有显著性影响,同时过量施氮增加了土壤硝态氮淋溶风险,故在有机质含量低的黄土高原南部旱地冬小麦种植中不宜施用高量氮肥,以减少土壤氮素残留和农业投入,达到保护环境和培肥土壤的目的。     

红壤旱地早熟型马铃薯高产与氮素高效的最优施氮量研究

  【目的】  研究不同施氮量下马铃薯的干物质积累、产量、氮肥吸收利用，结合土壤中无机氮在不同土层含量的变化，确定马铃薯产量和氮效率最优、环境风险最低的氮肥施用水平。  【方法】  试验于2018—2019年在南方典型红壤区旱地进行，供试品种荷兰15号为特早熟型马铃薯。设置N 0、60、120、150、180、210、240 kg/hm2，共7个氮肥水平。于成熟期，调查块茎产量和总干物质积累量测定氮素含量，同时取0—20、20—40、40—60 cm土层样品，分析铵态氮与硝态氮含量。  【结果】  施氮量显著影响红壤旱地马铃薯产量、干物质积累与氮肥吸收利用。马铃薯块茎产量随施氮量增加先增加后降低，均以施N 180 kg/hm2处理最高，达26250 kg/hm2 (2018年) 和27915 kg/hm2 (2019年)；秸秆氮素积累量随施氮量的增加显著增加，而块茎氮素积累量随施氮量增加先增加后降低，以施N 180 kg/hm2处理最高，为97.65 kg/hm2 (2018年) 和101.09 kg/hm2 (2019年)。氮素收获指数以N150 kg/hm2处理最高，而氮肥农学利用率和氮素回收率均以N180 kg/hm2处理最高，氮肥偏生产力则随施氮量的增加而显著降低。施氮显著提高土壤中的无机氮含量，不同施氮量对无机氮的含量和分布影响不同。施N 150 kg/hm2和N 180 kg/hm2处理增加的铵态氮主要分布在0—20 cm土层，且施N 180 kg/hm2处理的铵态氮含量显著高于施N150 kg/hm2处理，施N 150 kg/hm2处理又显著高于其他处理；而N 210 kg/hm2和N 240 kg/hm2处理增加的铵态氮主要分布在20—60 cm 土层，其铵态氮含量显著高于其他处理；在施N 0—180 kg/hm2范围内对土层中的硝态氮含量影响较小，施N 210 kg/hm2和N 240 kg/hm2处理显著增加了20—60 cm土层硝态氮含量。从无机氮总量看，施N 180 kg/hm2处理可显著增加0—20 cm土层的无机氮总量，而施N 210 kg/hm2处理和N 240 kg/hm2处理则显著提高了20—60 cm土层的无机氮含量。  【结论】  极早熟型马铃薯适宜的氮肥用量范围较窄，过低或者过高施氮都会显著降低其经济产量、氮素收获指数和农学效率。在红壤条件下，施N 180 kg/hm2可以显著增加0—20 cm土层中的铵态氮和无机氮含量，而不会增加20 cm以下土层的无机氮含量，超过此用氮量，则会显著增加土壤无机氮的向下迁移。因此，红壤旱地极早熟型马铃薯品种的适宜施氮水平为N 180 kg/hm2。     

施肥与灌水对硝态氮在土壤中残留的影响

通过田间试验研究不同施氮量与灌水量对春玉米和冬小麦田土壤中硝态氮分布与累积的影响,结果表明,春玉米收获后0~2 m土壤中累积硝态氮185.7~748.0 kg/hm2,其中1 m以上占57.9%~70.1%。由于施用氮肥而增加的硝态氮占施N量的1.8%(N 112.5 kg/hm2),50.7%(N 225 kg/hm2),56.7%(N 337.5 kg/hm2)和77.0%(N450 kg/hm2)。不施N和施N 112.5 kg/hm2时春玉米田土壤剖面没有明显累积峰;施N等于或高于225 kg/hm2时在60~80 cm土层有明显累积峰,施氮量高的峰值较高;施N 450 kg/hm2时在120~140 cm深度出现另一个累积高峰。冬小麦收获后土壤0~2 m硝态氮累积量为74.9~328.8 kg/hm2,其中1m以上占67.8%~90.7%。由于施用氮肥而增加的硝态氮占施N量的19.5%(N 112.5 kg/hm2),35.6%(N 225 kg/hm2),58.9%(N 337.5 kg/hm2)和56.4%(N 450 kg/hm2)。冬小麦田收获后土壤深层(1~2 m)没有明显的硝态氮累积,即使施氮量高达450 kg/hm2时也只在表层40 cm以上累积较多。不论是春玉米还是冬小麦,当生育期施氮量大于225 kg/hm2时0~2 m土层均有明显的硝态氮累积,施氮量高的累积量较高。施氮量是造成土壤中硝酸盐累积的主要因素,灌水量对春玉米田硝态氮的向下迁移有显著影响。     

Caimans,Capybaras, otters,manatees, and man in amazonia

Amazonia, the world's largest tropical rain forest, is often assumed to be a virtually untouched wilderness. The region is often referred to as a demographic void; there is on average only about one person per sq. km. Yet in response to international market forces, the hand of man has penetrated deep into the imposing forests. Since colonial times, wildlife, particularly along rivers, has been exploited on a large-scale basis for commercial purposes. This paper focuses on the effects of the trade on some aquatic animals.     

Hg, Cu, Pb, Cd, and Zn Accumulation in Macrophytes Growing in Tropical Wetlands

The concentrations of Hg, Cu, Pb, Cd, and Zn accumulated by regional macrophytes were investigated in three tropical wetlands in Colombia. The studied wetlands presented different degrees of metal contamination. Cu and Zn presented the highest concentrations in sediment. Metal accumulation by plants differed among species, sites, and tissues. Metals accumulated in macrophytes were mostly accumulated in root tissues, suggesting an exclusion strategy for metal tolerance. An exception was Hg, which was accumulated mainly in leaves. The ranges of mean metal concentrations were 0.035?C0.953 mg g^?1 Hg, 6.5?C250.3 mg g^?1 Cu, 0.059?C0.245 mg g^?1 Pb, 0.004?C0.066 mg g^?1 Cd, and 31.8?363.1 mg g^?1 Zn in roots and 0.033?C0.888 mg g^?1 Hg, 2.2?C70.7 mg g^?1 Cu, 0.005?C0.086 mg g^?1 Pb, 0.001?C0.03 mg g^?1 Cd, and 12.6?C140.4 mg g^?1 Zn in leaves. The scarce correlations registered between metal concentration in sediment and plant tissues indicate that metal concentrations in plants depend on several factors rather than on sediment concentration only. However, when Cu and Zn sediment concentrations increased, these metal concentrations in tissues also increased in Eichhornia crassipes, Ludwigia helminthorriza, and Polygonum punctatum. These species could be proposed as Cu and Zn phytoremediators. Even though macrophytes are important metal accumulators in wetlands, sediment is the main metal compartment due to the fact that its total mass is greater than the corresponding plant biomass in a given area.     

Selenium in Water,Sediment, Plants,Invertebrates, and Fish in the Blackfoot River Drainage

Nine stream sites in the Blackfoot River watershed in southeastern Idaho were sampled in September 2000 for water, surficial sediment, aquatic plants, aquatic invertebrates, and fish. Selenium was measured in these aquatic ecosystem components, and a hazard assessment was performed on the data. Water quality characteristics such as pH, hardness, and specific conductance were relatively uniform among the nine sites examined. Selenium was elevated in water, sediment, aquatic plants, aquatic invertebrates, and fish from several sites suggesting deposition in sediments and food web cycling through plants and invertebrates. Selenium was elevated to concentrations of concern in water at eight sites (>5 g/L), sediment at three sites (>2 g/g), aquatic plants at four sites (>4 g/g), aquatic invertebrates at five sites (>3 g/g), and fish at seven sites (>4 g/g in whole body). The hazard assessment of selenium in the aquatic environment suggested low hazard at Sheep Creek, moderate hazard at Trail Creek, upper Slug Creek, lower Slug Creek, and lower Blackfoot River, and high hazard at Angus Creek, upper East Mill Creek, lower East Mill Creek, and Dry Valley Creek. The results of this study are consistent with results of a previous investigation and indicate that selenium concentrations from the phosphate mining area of southeastern Idaho were sufficiently elevated in several ecosystem components to cause adverse effects to aquatic resources in the Blackfoot River watershed.     

Variation of N,P, K,Ca, Mg,Mn, Zn,and al in slash pine foliage

Abstract

The seasonal patterns of foliage nutrient concentrations and contents were monitored for two growing seasons in an 11‐year—old Pinus el1iottii stand. In the first growing season after needle initiation, N, P, K, Mg, and Zn concentrations decreased, but this was followed by an increase in the fall and winter months. Another drop in concentration of all elements, except P, occurred in the second growing season. Decreases in total contents indicated that this drop was a result of translocation to other tissues. In contrast to the mobile elements, the concentration and fascicle contents of Ca, Mn, and Al increased with aging of the needles.

Between‐tree variability was least for N, P, and Zn and the N, K, Mg, Mn, and Zn in the current foliage had consistently lower variation than that in the 1‐year‐old foliage. Between‐tree variation for K was lower in the winter than the spring.

For pine foliage, recommended sampling period for N, P, Mg, and Zn is mid to late summer and for the other elements it is late fall to late winter.

There are several sources of variation that influence the level of nutrients in tree foliage. The most important of these, apart from the tree nutrient status, are seasonal fluctuations, variation between trees, and age of needles . Smaller sources of variation are associated with position of the needles within the crown, diurnal changes, year to year variation, and analytical errors^1,2. These variables must be studied in order to develop suitable sampling techniques and in Pinus this has been undertaken for P. banksiana ¹, P. taeda ³, P. strobus ⁴, P. resinosa ⁴, P. sylvestris ⁵, and P. radiata ^6,7. However, foliage sampling has not been studied in detail for slash pine (Pinus elliottii Englem var. elliottii) and earlier studies with other pines have been largely confined to temperate or cool climates.

This study reports the variation in elemental concentrations with season, age of foliage, and between slash pine trees growing in a subtropical climate in Florida.     

Arsenic Concentrations in Water,Soil, Milk and Forage in Comarca Lagunera,Mexico

Arsenic levels were determined in seventy three samples of well water, and in fifty samples of soil, forage and cow's milk collected at the most important dairy farms of the Comarca Lagunera located in Coahuila and Durango, Mexico, region naturally rich in As. The total inorganic arsenic concentration in well water ranged from 7 to 740 μg L-1 and about ninety percent of the total arsenic was found as As(V). The agricultural soil texture of the sampled area was sandy clay loam type with total arsenic levels up to 30 μg g-1, however, the extractable arsenic was not higher than 12% of the total and it was higher in the 0–30 cm depth horizon. In alfalfa, the most important crop, the total aresenic ranged from 0.24 to 3.16 μg g-1, with 40% of it accumulated at the root level. Significant correlations (p=0.05) were obtained between arsenic (III), (V) and total inorganic arsenic in groundwater with arsenic in soil (0–30 cm depth), and with arsenic in alfalfa (leaves and roots). It was also found a good correlation between extractable arsenic in soil with As concentrations in alfalfa (roots). Arsenic concentrations found in milk ranged from <0.9 to 27.4 ng g-1. The cow's milk biotransfer factor for arsenic was up to 6 × 10-4, applying a pharmacokinetic approach. It was associated with the exposure not only to food but also to water arsenic.     

Extractors for barium,cadmium, copper,nickel, and zinc in tropical soils

ABSTRACT

The accumulation of potentially toxic elements (PTEs) in the soil can pose risks to human health, and precise risk assessment dealing with the production and consumption of plants is required. The 0.43 M of nitric acid (HNO?) solution was suggested by the International Organization for Standardization for reactive fraction of PTEs in the soil. The efficiency of some extractors was evaluated in tropical soils. Contents of barium (Ba), cadmium (Cd), copper (Cu), nickel (Ni) and zinc (Zn) were extracted in accordance with the methods of Environmental Protection Agency (EPA) 3051A, Aqua Regia, Diethylenetriaminepentaacetic acid (DTPA), Mehlich-1, Mehlich-3, 0.43 M HNO? and 0.01 M of calcium chloride (CaCl?), and these contents correlated with the contents of PTEs in roots, shoots, and fruits of vegetables. Mehlich-3 had the highest correlation with Ni and Zn contents extracted by the plants. Contents extracted with 0.43 M HNO? had high correlation with the amounts extracted by DTPA and Mehlich-3, as well as with the amounts of PTEs accumulated by plants.     

Fluxes of Cu,Zn, Pb,Cd, Cr,and Ni in temperate forest ecosystems

The literature on the fluxes of six heavy metals in temperate forest ecosystems is reviewed. Special attention is given to wet and dry deposition and internal flux, to metal budgets for ecosystems and soils, to concentrations in aqueous compartments of the ecosystem and to speciation in soil solutions. Metal fluxes are discussed in relation to pollution load, soil type, tree species and land use. The mobility of Cu and Pb is strongly dependent on the solubility of organic matter. These metals are commonly accumulated in forest soils. Zinc, Cd and Ni are greatly influenced by soil acidity and are often lost in considerable amounts from acidified soils. Chromium is often at balance in forest ecosystems. Implications for metal solubility and budgets in forest soils are discussed in connection with an increase in soil acidification.     

Distribution of Zinc,Manganese, Copper,Cobalt, and Nickel in Andosols Profiles

The distribution of zinc, manganese, copper, cobalt, and nickel in Andosols was investigated. Sixty nine soil samples were collected from different horizons of an Andosols profile in Miyakonojo Basin in south Kyushu, Japan, The total contents of heavy metals were determined by digestion and four extraction solutions, 1 M NH₄Ac (ammonium acetate) pH 4.5, 0.1 M HCl, 0.01 M EDTA (ethylenediaminetetraacetic acid) pH 6.5, and 0.005 M DTPA (diethylenetri-aminepentaacetic acid) pH 7.3 were used to determine the contents of available Zn, Mn, Cu, Co, and Ni in Andosols in relation to the organic carbon content. The results of the extraction analysis showed that by the use of 0.1 M H Cl high value of extracted heavy metals in the upper layers of the humus horizons were obtained while EDTA extraction yielded a large amount of the above mentioned metals in the high humus horizons. The extractable heavy metals contents were high and these metals closely related to the organic carbon content mostly in the humus horizons in the profile. Where, biocycling process may play an important role in the concentration of heavy metals. Based on the study, it was found that the total content of Zn increased towards the C horizons or pumice layers in the soil profile. Such a trend was also found in the case of the Mn content. While the Cu content in the humus horizons was much higher in the upper part of each humus horizon. According to this study the distribution of heavy metals, Cu (organic matter complexes) in the Andosols profile was more stable than that of Zn (organic matter complexes) in soils. It was shown that Zn in the surface humus horizon was enriched but that some amount was leached under buried conditions. The same phenomenon was also observed in the distribution of Mn in the profile. The movement of Co and Ni in the soil profile was limited, as evidenced by the sharp reduction in the concentrations of these two metals in buried soils.

Hence, it is concluded that the distribution of Zn, Mn, Cu, Co, and Ni was considerably higher in the humus horizons of the Andosols profiles.