广西水稻米质研究

对广西46个水稻品种稻米的外观、碾米、蒸煮食味及营养品质进行了分析,结果表明,稻米长度以中长、短两种类型为主,形状以适中品种占多数,垩白大、小类型偏多;碾米品质普遍优良;糊化温度以中、低型为主,胶稠度软型品种少,硬型、中型品种居多;直链淀粉中等类型少;蛋白质、粗脂肪含量中等。     

影响米粉品质的稻米质量指标分析

大米原料的质量是影响米粉加工性能、烹煮性能和感官品质的重要因素。影响米粉品质的大米原料质量指标主要有直链淀粉含量、胶稠度、糊化温度、蛋白质含量和储藏时间等,本文对近年来有关这些指标的研究进行了归纳总结,对各项指标对米粉品质的影响进行了逐一评述。通过总结分析可以得出,直链淀粉含量较高的籼米适宜于生产米粉;胶稠度与稻米其他各项指标有显著的相关性,可以作为选取米粉原料的主要指标;不同种类大米的糊化温度是不同的,可以根据糊化温度来指导米粉生产,避免淀粉过度糊化和米粉品质降低;蛋白质含量不仅决定大米的营养品质,也影响大米制品外观、加工和食用品质;使用陈化一段时间的大米制作米粉能够提升米粉品质。     

氮肥对稻米垩白及蒸煮食味品质特性的影响

利用稻米品质特性不同的 7个粳稻品种 ,研究了氮肥对稻米垩白率、理化特性及淀粉谱特性的影响。结果表明 ,随着氮肥施用量的增加 ,稻米垩白率和直链淀粉含量逐渐降低 ,胶稠度变短 ,蛋白质含量提高 ,而在稻米淀粉谱特性中除糊化开始温度略提高外 ,其余特性均降低或变小 ;在水稻全生育期施同等量的氮肥时 ,与生育前期追施氮肥相比 ,抽穗期追施氮肥 ,其稻米的垩白率和直链淀粉含量降低 ,胶稠度变短 ,蛋白质含量提高 ,但对稻米淀粉谱特性的影响很小。品质特性对氮肥的反应敏感程度品种间有差异。     

广西早籼品种(组合)的稻米品质状况及改良目标

广西多数早籼品种(组合)的糙米率、精米率、粒长、粒形、糊化温度、胶稠度、蛋白质含量等指标。达到了农业部(NY122-86)二级优质食用稻米标准。但完整米率的达标率偏低,胚乳的垩白程度偏大,垩白粒率偏高,直链淀粉含量偏高,米粒的蒸煮延长性差。今后,早籼稻的品质育种应把提高完整米率,降低直链淀粉含量和提高胚乳的透明度作为品质改良的主攻目标。     

粳稻粒位间淀粉RVA谱特征与其它品质性状的关系

为了明确RVA谱特征值在评价稻米品质中的作用,本文选用不同穗型水稻为材料,按照穗部位置分为27个粒位,研究了不同粒位上淀粉RVA谱特征与其它品质性状的关系。结果表明碾米品质中的整精米率和粒长与峰值黏度、热浆黏度和崩解值3个特征值有极显著正相关关系。外观品质中的垩白率、垩白度和营养品质中的总蛋白质含量、清蛋白含量、醇溶蛋白含量、谷蛋白含量、脂肪酸含量都与峰值黏度、热浆黏度、崩解值呈极显著负相关,与消减值极显著正相关;球蛋白含量则表现相反规律。蒸煮食味品质中的食味值、直链淀粉含量和胶稠度与峰值黏度、热浆黏度、崩解值3个特征参数呈极显著正相关,与消减值呈极显著负相关,尤其是与峰值黏度和崩解值2个参数的相关系数较大。淀粉RVA谱特征值能密切反映稻米品质的好坏,穗下部二次枝梗籽粒的峰值黏度和崩解值的大小可以作为优质粳稻辅助选择的首选指标。     

氮素穗肥对不同品种稻米品质性状的影响

以4种不同类型的稻米为材料,采用4个不同的氮素穗肥处理,利用黏度分析仪和XRD技术测定其淀粉特性,探索氮素穗肥对稻米品质及淀粉特性的影响。试验结果表明,氮素穗肥对稻米蛋白质、直链淀粉含量、支/直比和胶稠度的影响,品种间存在差异。各品种稻米的黏滞谱曲线随着氮肥的增加均呈整体下降趋势;相对粳稻而言,杂交籼稻和糯稻黏滞谱曲线在不同的氮肥处理下降幅度较小。各品种稻米淀粉的相对结晶度均表现为随氮肥施用量的增加而减小,17和18的两个衍射峰的分离状态逐渐减小。氮素穗肥施用量与稻米品质和淀粉特性关系密切,可通过调控氮素穗肥水平来改善稻米品质。     

结实期温度对稻米理化特性及淀粉谱特性的影响

利用玻璃室内的高温和室外的常温，研究了结实期温度对稻米理化特性和淀粉谱特性的.影响结果表明，随着结实期温度的升高，稻米的糊化温度和蛋白质含量提高，胶稠度变化，而直链淀粉含量下降；相关分析表明，结实期日平均气温与胶稠度和蛋白质含量呈正相关，而与碱消值和直链淀粉含量呈负相关，糊化开始温度随结实期温度的升高而提高，但最高粘度、最低粘度及下降粘度值的变化因品种生态类型不同而异，即在高温条件下结实的稻米与在常温条件下结裨稻米相比，其最高粘度，最低粘度以及下降粘度值，早熟品种前者比后者大，而晚熟品种前者比后者小。     

不同杂交中稻品种在川南再生稻区的两季产量及头季稻米品质差异

川南高温伏旱区杂交中稻因高温逼熟,产量低且稻米品质较差。杂交中稻蓄留再生稻是充分利用秋季温光资源提高稻谷产量和改善品质的重要途径。以2015—2016年审定的12个杂交中稻新品种为材料,分别在川南有一定生态代表性的隆昌、江安、富顺、宜宾4个地点采取统一的栽培技术方案,研究了杂交中稻品种与环境对头季稻及其再生稻产量与头季稻米品质的影响。结果表明, 4个地点间的头季稻产量、再生稻产量和两季总产量差异达极显著水平。12个水稻品种的头季稻产量、再生稻产量均达极显著差异,而两季总产量间差异则不显著。其中有7个品种(‘创两优华占’‘内5优907’‘宜香4245’‘泸香优104’‘花优357’‘内6A/绵恢138’‘隆两优1813’)两季产量在11 000kg·hm~(-2)以上。6个稻米品质性状在4个地点间的差异除胶稠度不显著外,其余5个性状均达极显著水平,以宜宾和江安2个点的米质稍好。12个水稻品种在4个地点间的品质差异均达极显著水平,综合考虑总体米质相对较好的为‘创两优华占’‘隆两优1813’两个品种。再生稻产量和头季稻产量间呈显著负相关关系,头季稻产量与整精米率、垩白粒率、垩白度相关不显著。说明川南特定生态下需要改良的整精米率、垩白粒率、垩白度3个米质性状与高产并没有明显矛盾,长宽比大的品种有利于降低垩白粒率、垩白度、直链淀粉含量,提高胶稠度。     

蛋白质中二硫键对稻米淀粉黏滞性的影响

在6个不同品种的稻米粉中添加二硫苏糖醇以打破米粉中蛋白质的二硫键,用快速粘度分析仪测定淀粉的黏滞谱,研究稻米中蛋白质二硫键对稻米黏滞性的影响。结果显示,样品中添加DTT（二硫苏糖醇）后,糊化温度降低,糯稻的峰值粘度下降,其他样品的峰值粘度上升,而消减值极显著降低。蛋白质影响米饭的蒸煮食味品质,可能是二硫键结合形成的蛋白质网络的变化引起稻米吸水性改变而起作用。     

近地层臭氧浓度增高对稻米品质的影响: FACE研究

利用稻田开放式空气中臭氧浓度增加(FACE,Free Air gas Concentration Enrichment)平台,以常规粳稻‘武粳15’和杂交粳稻‘陵风优18’为供试材料,设置大气背景臭氧浓度(Ambient)和高臭氧浓度(比Ambient增高21%,模拟21世纪中叶臭氧浓度)两个水平,研究臭氧胁迫对大田生长水稻成熟期稻米加工、外观、蒸煮/食味和营养品质的影响及其种间差异。结果表明,近地层臭氧浓度增高使稻米糙米率、精米率和整精米率均不同程度下降,其中精米率降幅达显著水平。与Ambient相比,臭氧胁迫使两品种稻米垩白米率、垩白大小和垩白度平均分别增加15.0%(P=0.10)、42.0%(P0.05)和60.5%(P0.05)。臭氧胁迫使稻米胶稠度平均降低7.1%(P0.05),但对两品种稻米直链淀粉和糊化温度均无显著影响。稻米RVA谱测定结果显示,臭氧胁迫对稻米最高黏度值、崩解值、冷胶黏度值、消减值和回复值均无显著影响。臭氧胁迫使两品种稻米蛋白质浓度呈增加趋势,但均未达显著水平。方差分析表明,多数情况下,两品种间稻米诸品质性状存在显著差异,但品种与臭氧的互作对所有测定指标均无显著影响,说明两品种稻米品质对臭氧胁迫的响应无明显差异。本试验在开放稻田条件下运行,适度臭氧胁迫使稻米垩白明显增加,胶稠度显著下降,但对其他米质指标影响较小,两品种趋势一致。     

辐照改良水稻淀粉特性的研究

以相似AAC的不同类型水稻 (籼稻、粳稻和杂交稻 )和不同AAC早籼稻品种为材料 ,研究了辐照对淀粉粘滞性、理化品质和淀粉颗粒的影响。研究表明 ,最高粘度 (PKV)、热浆粘度 (HPV)、冷胶粘度 (CPV)、消减值 (SBV)和回复值 (CSV)等 5个RVA谱特征参数随剂量增加均显著下降。辐照明显降低了低AAC品种、糯稻和中等AAC品种的AAC ,但对高AAC品种的AAC影响不显著。辐照对碱消值 (ASV)无影响 ,但最高粘度时间 (PKT)随剂量增加而下降。胶稠度尤其是高AAC的胶稠度 (GC)随剂量增加明显提高。胚乳内淀粉颗粒经辐照处理也有所变形。结果表明 ,辐照可提高稻米食用和蒸煮品质。     

Starch physicochemical properties and their associations with microsatellite alleles of starch-synthesizing genes in a rice RIL population

The physicochemical properties of starch, such as apparent amylose content, gelatinization temperature, and pasting viscosities, determine the eating, cooking, and processing qualities of various products of rice. A recombinant inbred line (RIL) population derived from the reciprocal cross of Lemont (a premium high-quality tropical japonica rice) and Jiayu 293 (a high-yield but low-quality indica rice) was used to test the association of microsatellite markers of starch-synthesizing genes with starch quality parameters. The results confirmed the association of Wx and starch synthase I (SSI) alleles with various starch properties measured in rice flour. However, the starch properties were not associated with the starch branching enzyme 1 (SBE 1) gene alleles.     

Starch properties of mutant rice high in resistant starch

As the staple food of over half the world's population, hot cooked rice high in resistant starch (RS) is of particular interest, which will have greater impact in the dietary prevention of diabetes and hyperlipidemia. A mutant rice high in RS in hot cooked rice, described as RS111, was comparatively studied with the wild type and common rice. Despite obviously low RS content in the raw milled rice, the RS content in the hot cooked rice of mutant RS111 was significantly higher than that of the wild type and common rice and, correspondingly, in vitro starch hydrolysis by porcine pancreatic alpha-amylase tends to be incomplete with low hydrolysis extent for the cooked mutant rice high in RS. Obvious differences in physicochemical properties, starch granule morphology, pasting properties, thermal properties, and X-ray diffraction pattern were observed among the mutant RS111, wild type, and common indica rice. The high-RS mutant was characterized by significantly higher apparent amylose content and crude lipid content, higher percentage of oval-shaped granules and bigger oval size, reduced paste viscosity, and low onset temperature, peak temperature, final temperature, enthalpy of gelatinization, and crystallinity.     

超声处理对糯米与非糯米淀粉结构和消化性的影响

为了探究超声处理对糯米和非糯米淀粉颗粒结构和功能特征的影响,改善稻米淀粉的品质,本试验以糯稻(Y26)、非糯稻(Y9)淀粉为材料,对其进行超声处理,分析处理后淀粉的结构热力学性质及消化特性。结果表明,与对照组相比,两种淀粉经超声处理后各性质变化趋势相似,淀粉颗粒表面产生凹陷、直链淀粉含量升高、结晶度下降、傅立叶变换红外光谱(FTIR)的1 047 cm^-1/1 022 cm^-1比值先增加后降低,弹性模量(G')降低,凝胶强度减弱,糊化终止温度与起始温度之差(Tc-To)减小,快速消化淀粉(RDS)和抗性淀粉(RS)含量增加,但两种淀粉的变化程度有一定差异。本研究初步奠定了生产中根据对淀粉性质的需求来确定合适超声处理条件的理论基础。     

Physicochemical Properties and In Vitro Starch Digestibility of Cooked Rice from Commercially Available Cultivars in Canada

The influence of amylose content, cooking, and storage on starch structure, thermal behaviors, pasting properties, and rapidly digestible starch (RDS), slowly digestible starch (SDS), and resistant starch (RS) in different commercial rice cultivars was investigated. Long grain rice with high‐amylose content had a higher gelatinization temperature and a lower gelatinization enthalpy than the other rice cultivars with intermediate amylose content (Arborio and Calrose) and waxy type (glutinous). The intensity ratio of 1047/1022 cm^–1 determined by Fourier Transform Infrared (FT‐IR), which indicated the ordered structure in starch granules, was the highest in glutinous and the lowest in long grain. Results from Rapid ViscoAnalyser (RVA) showed that the rice cultivar with higher amylose content had lower peak viscosity and breakdown, but higher pasting temperature, setback, and final viscosity. The RDS content was 28.1, 38.6, 41.5, and 57.5% in long grain, Arborio, Calrose, and glutinous rice, respectively, which was inversely related to amylose content. However, the SDS and RS contents were positively correlated with amylose content. During storage of cooked rice, long grain showed a continuous increase in pasting viscosity, while glutinous exhibited the sharp cold‐water swelling peak. The retrogradation rate was greater in rice cultivars with high amylose content. The ratio of 1047/1022 cm^–1 was substantially decreased by cooking and then increased during storage of cooked rice due to the crystalline structure, newly formed by retrogradation. Storage of cooked rice decreased RDS content and increased SDS content in all rice cultivars. However, no increase in RS content during storage was observed. The enthalpy for retrogradation and the intensity ratio 1047/1022 cm^–1 during storage were correlated negatively with RDS and positively with SDS (P ≤ 0.01).     

水稻低淀粉粘滞突变体的理化特性和淀粉结构

以水稻低淀粉粘滞突变体RSV-1及其野生型品种II-32B为材料,研究了低淀粉粘滞突变的理化特性和淀粉结构。结果表明,低淀粉粘滞突变体的最高粘度、热浆粘度和冷胶粘度明显小于野生型,但表观直链淀粉含量、胶稠度、碱消值均大于野生型,达最高粘度时间接近,糊化过程中所需能量较低,糊化过程较长;淀粉晶体表现为C型结构,淀粉颗粒与野生型的正六面体颗粒完全不同,呈大小不等的无规则形,淀粉晶体及颗粒结构的改变可能是导致淀粉粘滞性下降及其他理化指标改变的重要原因。     

土壤水分供给对不同水稻的产量构成及其淀粉品质的影响

在人工气候室和网室条件下,测定了4个水分处理下2个籼型水稻材料中9B和金早47的株产量和淀粉品质。结果表明,中9B在水分胁迫下株产量显著降低主要原因在于有效穗数显著减少,而金早47随水分胁迫加重株产量显著降低,原因是水稻穗型变小,总粒数和实粒数显著减小。在水分胁迫下,2个水稻品种在产量构成中的不同表现,反映了不同水稻品...     

抽穗扬花期低温胁迫对双季晚稻生理特性的影响

为探究抽穗扬花期低温胁迫对不同晚稻品种生理特性的影响,本研究以2个耐冷品种(岳优27、昌粳225)和2个冷敏感品种(泰丰优208、黄华占)为试验材料,于抽穗扬花期在人工气候室进行低温处理,分析其光合特性、抗氧化酶活性、穗部性状与稻米品质等。结果表明,随着低温处理时间的延长,各晚稻品种花粉活力均降低,除过氧化氢酶(CAT)外的抗氧化酶活性、丙二醛(MDA)及可溶性蛋白(SP)含量都有不同程度的增加,其中超氧化物歧化酶(SOD)活性和SP含量呈先增加后降低的趋势,而CAT活性呈先降低后增加的趋势,结实率和千粒重呈降低趋势。低温胁迫也会影响稻米品质及稻米RVA值,具体表现为随着低温处理时间的延长,各晚稻品种精米率降低,垩白粒率、垩白度和胶稠度增加,直链淀粉与粗蛋白含量呈先增加后降低的趋势;峰值黏度、崩解值逐渐降低,冷胶黏度、回复值和消减值逐渐升高。相较于冷敏感品种泰丰优208和黄华占,耐冷品种岳优27和昌粳225的抗氧化酶活性和SP含量较高,MDA含量较低;结实率和千粒重降低幅度较小,花粉活性降低幅度也较小,其中在第5天昌粳225降幅最小,为4.38%;稻米品质中精米率、垩白粒率、胶稠度、直链淀粉含量和蛋白质含量变化幅度较小,而垩白度增加幅度较大,RVA谱中峰值黏度降低幅度较大,热浆黏度反应较慢,而冷胶黏度和回复值较先达到最大值,崩解值和消解值的变化幅度较小。综上,耐冷品种岳优27和昌粳225抽穗扬花期相较于冷敏感品种泰丰优208和黄华占表现出较强耐受低温胁迫的能力。本研究结果可为抽穗扬花期耐低温双季晚稻的选育与栽培提供一定的理论参考。     

Impact of Elevated Nighttime Air Temperatures During Kernel Development on Starch Properties of Field‐Grown Rice

The structural features of starch were examined to better understand the causes of variability in rice quality resulting from nighttime air temperature (NTAT) incidence during kernel development. Starch samples were isolated from head rice of four cultivars (Bengal, Cypress, LaGrue, and XL723) field‐grown in four Arkansas locations (Keiser, Pine Tree, Rohwer, and Stuttgart) in 2009 and 2010. Average NTATs recorded during the grain‐filling stages of rice reproductive growth in the four locations were 3.0–8.4°C greater in 2010 than 2009. Elevated NTATs altered the deposition of starch in the rice endosperm. Means pooled across cultivars and locations showed that amylose content was 3.1% (percentage points) less for the 2010 sample set. The elevated NTATs in 2010 resulted in a decrease in the percentage of amylopectin short chains (DP ≤ 18) and a corresponding increase in the percentage of long chains (DP ≥ 19) by an average of 1.3% (percentage points). The greater NTATs in 2010 also produced greater starch paste peak, final, and breakdown viscosities, whereas setback and total setback viscosities decreased. Changes in paste viscosity were highly correlated with the changes in the proportion of amylose and amylopectin. Onset gelatinization temperature was greater by 3.5°C, gelatinization enthalpy by 1.3 J/g, and relative crystallinity by 1.5% (percentage points) for the 2010 sample set. Changes in gelatinization parameters and granule relative crystallinity were highly correlated with the changes in amylopectin chain‐length distribution. Year × cultivar × location interaction effects were statistically insignificant, indicating that the four cultivars evaluated all showed some degree of susceptibility to the effects of temperature incidence during kernel development, regardless of the growing location.