Klimaeinfluß auf Lignin und Polysaccharide in Partikelgrößen-Fraktionen zonaler Steppenböden Rußlands

Climatic effect on lignin and polysaccharides in particle-size fractions of zonal steppe soils, Russia Zonal soils derived from similar parent materials are suitable for investigating the question, whether and how climate affects soil organic matter properties. For this reason we sampled 10 native surface (0—10 cm) and subsurface (ca. 50—60 cm) soil horizons in the native steppe and forest steppe of Russia. Polysaccharides and the vanillyl, syringyl and cinnamyl structural units of lignin (VSC) were determined in the fine earth (< 2 mm) as well as in clay (< 2 μm) and silt (2—20 μm) fractions. As the ratio of mean annual precipitation to potential evaporation (N/V) decreased, the concentrations of polysaccharides tended to decrease in the subsoil horizons. This was indicated most clearly for the silt fractions (r = 0.98**). In contrast, the VSC contents (in g kg^—1 organic C) of the subsoils increased as N/V decreased (r = —0.92*), resulting in increasing VSC/polysaccharide ratios of the subsoil horizons with decreasing N/V ratio (r = —0.94*). It is suggested that production of polysaccharides or their transport into the mineral subsoil or both is favored at sites with wide rather than narrow N/V ratio, whereas lignin might be selectively enriched during intense soil organic matter decay at the Southern sites.     

Black carbon in the zonal steppe soils of Russia

Black Mollisols are typically rich in charred organic matter, however, little is known about the zonal distribution of black C (BC) in steppe soils. In this study, we used benzene polycarboxylic acids (BPCA) as specific markers for BC in particle‐size fractions of depth profiles in several zonal soils (Greyzem, Phaeozem, Chernozem, Kastanozem) of the Russian steppe. In addition, liquid‐state ¹³C‐NMR spectra were obtained on the alkaline‐soluble soil organic matter (SOM). The results showed that both the content and depth distribution of BC varies in the different soil types; the concentration of BC in the bulk top soils being closely related to the aromaticity of the SOM (r² = 0.98 for the native topsoils, 0.83 for top‐ and subsurface soils). Especially the Chernozems were rich in aromatic SOM, which partly contained more than 17% BC of total C, most of which being allocated in the mineral fractions. Long‐term arable cropping did not reduce the BC contents of the surface soil, though it did promote the enrichment of BC in the silt fractions. The same shift was detected as soil depth increased. We conclude that BC is not fully inert in these soils, but apparently can be preserved in the silt as decomposition of SOM increased, i.e., it accumulates exactly in that fraction, which has been formerly assigned to contain old, aromatic C.     

Physical Properties of Urban Soils in Rostov Agglomeration

Transformation of particle-size composition, structure, and density of soils upon urbopedogenesis is considered for Rostov agglomeration. Various soils are compared by horizons. It is found that the share of sand fractions increases in upper and middle horizons of migration–segregation chernozems (Calcic Chernozem (Hyperhumic, Loamic)), above all, at the expense of particles of 0.05—0.001 mm in size; with the coarse medium sand fraction 1–0.25 mm being diagnostic for urbopedogenesis. The reason is the introduction of sandy particles upon urban construction, arranging water conduits and other utility lines, as well as the use of icing-control sandy mixtures. The Dolgov-Bakhtin schedule appears to be the most appropriate for assessing the structure of urban soils. Dry sieving testified to the decreasing amount of agriculturally valuable aggregates in all compared pairs of horizons in the sequence of urban soils: under forest vegetation → under steppe vegetation → in the buried massif of urbosoils. The water stability of aggregates decreases in the sequence: soils under steppe vegetation → buried horizons of urbosoils → soils under forest vegetation. The following sequence of urbic horizons (UR and RAT) shows a decrease in the share of agriculturally valuable fractions and an increase in their water stability: heavy-textured UR → light-textured UR → RAT. The density of natural soils varies insignificantly within the city territory, with its urbostratified soils (Calcic Chernozem Novic (Technic Loamic) in residential areas often manifesting the maximal density.     

Influence of fertilization and organic amendments on organic‐carbon fractions in Heilu soil on the loess plateau of China

The influence of fertilization on organic‐carbon fractions separated by density and particle size in Heilu soil (Calcic Kastanozems, FAO) was investigated in a 20‐year (1979–1999) long‐term experiment on the Loess Plateau of China. Compared to an unfertilized treatment, N application alone did not increase total organic carbon (TOC) and its fractions of density and particle size. However, the treatment of N + P fertilization significantly increased salty‐solution–soluble organic carbon (SSOC), microbial biomass C (MB‐C), and organic C associated with fine silt. When manure was applied alone and in combination with N and P fertilizer, the light fraction of organic C (LFOC), SSOC, and MB‐C were increased significantly, and the TOC was as high as that of a native Heilu soil. Organic C associated with different particle‐size fractions was also increased significantly, and the allocation of C among the fractions was altered: the proportions of C in sand (>50 μm), coarse‐silt (20–50 μm), and fine‐clay (<0.2 μm) fractions were increased whereas fine‐silt (2–20 μm) and coarse‐clay (0.2–2 μm) fractions were decreased. It is concluded that N fertilizer alone is not capable of restoring organic‐matter content in the Heilu soils of the Loess Plateau and that C‐containing material like manure and straw is necessary to produce significant increase in soil organic carbon in these soils.     

Assessment of Environmentally Harmful Nitrogen Surplus by 0.01 M Calcium Chloride Soil Extractant in Long-Term Experiments

Calcium chloride–extractable (1:10 soil/solution ratio, w/v) nitrate nitrogen (NO₃-N), ammonium nitrogen (NH₄-N), organic nitrogen (EON), and N_total fractions were measured in two long-term experiments. Both experiments are located in the Great Hungarian Plain. The first experiment was established on a Luvic Phaeozem soil and the second experiment was on a Calcic Chernozem soil. Researchers investigated the effects of long-term fertilization and irrigation on the amounts of nitrogen (N) fractions. Nitrogen rates caused significant increase in NO₃-N and N_total fractions. These fractions were in close correlation (r = 0.87; r = 0.88) with nitrogen balance. The nitrate content of the 200-cm-deep soil layer of soils under different nitrogen- and water-supply conditions were also studied. As an effect of N overfertilization, the maximum nitrate accumulation on nonirrigated plots occurred in the 200-cm depth, whereas on irrigated plots a nitrate accumulation layer could not be observed in the 200-cm-deep soil layer.     

Identification of carbonate pedofeatures of different ages in modern chernozems

Carbonate pedofeatures of three chernozemic soils developed from loesslike loams in the foreststeppe zone of Lipetsk oblast under fallow plot (Luvic Chernozem (Clayic, Pachic)) and under forest (Calcic Chernozem (Clayic, Pachic)) and in the steppe zone of Dnepropetrovsk oblast (Calcic Chernozem (Episiltic, Endoclayic, Pachic)) were studied in the field and laboratory with the use of a set of methods, including the radiocarbon method, mass spectrometry, and micro- and submicromorphology. The morphological diversity of carbonate pedofeatures in these soils was represented by carbonate veins, coatings, disperse carbonates (carbonate impregnations), soft masses (beloglazka), and concretions. In the forest-steppe soils, disperse carbonates and soft masses were absent. The radiocarbon age of carbonate pedofeatures in the forest-steppe soils varied within a relatively narrow range of 3–4.3 ka cal BP with a tendency for a younger age of carbonate concretions subjected to destruction (geodes). In the steppe chernozem, this range was larger, and the ¹⁴C ages of different forms of carbonate pedofeatures were different. Thus, soft masses (beloglazka) had the age of 5.5–6 ka cal BP; disperse carbonates, 17.5–18.5 ka cal BP; and hard carbonate concretions, 26–27 ka cal BP. Data on δ¹³C demonstrated that the isotopic composition of carbon in virtually all the “nonlabile” carbonate pedofeatures does not correspond to the isotopic composition of carbon of the modern soil organic matter. It was shown that the studied chernozemic soils are polygenetic formations containing carbonate pedofeatures of different ages: (a) recent (currently growing), (b) relict, and (c) inherited pedofeatures. The latter group represents complex pedofeatures that include ancient fragments integrated in younger pedofeatures, e.g., the Holocene soft carbonate nodules with inclusions of fragments of the ancient microcodium.     

有机肥对棕壤不同粒级有机碳和氮的影响

采集棕壤长期肥料定位试验站不施肥和施用不同用量有机肥的土壤,通过超声波分散—离心分离得到细黏粒(<0.2μm)、粗黏粒(0.2～2μm)、粉粒(2～53μm)、细砂粒(53～250μm)和粗砂粒(250～2000μm)5个颗粒级别后,分析全土及不同粒级中土壤有机碳和氮并进行含量与分布的比较。结果表明,有机质主要分布于黏粒级中,其含量占全土有机碳的42.8%、全氮的58.3%,碳氮比随着粒级的增加而逐渐增大,表明氮易于在小粒级中富集。长期施用有机肥后,全土及各粒级有机碳和氮含量均有显著增加;砂粒级中有机碳和氮的富集系数升高,黏粒级中富集系数降低,粉粒级和砂粒级中的碳氮比降低。增加有机肥的用量加强了全土和各粒级对有机碳和氮的积累,同时加强了粉粒级和砂粒级碳氮比降低的程度。     

The influence of different fertilization practices on concentrations of organic carbon and total nitrogen in particle-size fractions during 34 years of a soil formation experiment in loamy marl

Summary The concentrations of organic C and total N in five different particle-size fractions were studied under different mineral and organic fertilizer regimens by examining soil samples from the 34-year-old soil-formation pot experiment Hu 3 in Rostock. The C and N concentrations were generally highest in the clay fraction and decreased in the order medium silt >fine silt >coarse silt and sand. For nearly all years and size fractions the following order was obtained for C and N concentrations under the various fertilizer regimens: Compost >farmyard manure >straw + mineral fertilizer >mineral fertilizer. The various particle-size fractions and fertilizer regimens differed in the development of soil organic matter levels. Consequently, characteristic redistributions were found in the proportions of C and N in the various particle-size fractions, particularly after organic fertilizer was no longer applied (years 20–34). This experimental phase was characterized by decreased organic C and increased total N concentrations, and increased proportions of C and N in the clay-size at the expense of the sand fractions.     

Straw incorporation and soil organic matter in macro-aggregates and particle size separates

Two field experiments in which straw has been removed or incorporated for 17 yr (loamy sand) and 10 yr (sandy clay loam) were sampled to examine the effect of straw on the C and N contents in whole soil samples, macro-aggregate fractions and primary particle-size separates. The particle size composition of the aggregate fractions was determined. Aggregates were isolated by dry sieving. Straw incorporation increased the number of 1–20 mm aggregates in the loamy sand but no effect was noted in the sandy clay loam. Straw had no effect on the particle size composition of the various aggregate fractions. After correction for loose sand that accumulated in the aggregate fractions during dry sieving, macro-aggregates appeared to be enriched in clay and silt compared with whole soil samples. Because of the possible detachment of sand particles from the exterior surface of aggregates during sieving operations, it was inferred that the particle size composition of macro-aggregates is similar to that of the bulk soil. The organic matter contents of the aggregate fractions were closely correlated with their clay + silt contents. Differences in the organic matter content of clay isolated from whole soil samples and aggregate fractions were generally small. This was also true for the silt-size separates. In both soils, straw incorporation increased the organic matter content of nearly all clay and silt separates; for silt this was generally twice that observed for clay. The amounts of soil C, derived from straw, left in the loamy sand and sandy clay loam at the time of sampling were 4.4 and 4.5 t ha^?1, corresponding to 12 and 21% of the straw C added. The C/N ratios of the straw-derived soil organic matter were 11 and 12 for the loamy sand and sandy clay loam, respectively.     

Carbon storage in loess derived surface soils from Central Germany: Influence of mineral phase variables

The influence of the soil mineral phase on organic matter storage was studied in loess derived surface soils of Central Germany. The seven soils were developed to different genetic stages. The carbon content of the bulk soils ranged from 8.7 to 19.7 g kg^—1. Clay mineralogy was confirmed to be constant, with illite contents > 80 %. Both, specific surface area (SSA, BET‐N₂‐method) and cation exchange capacity (CEC) of bulk soils after carbon removal were better predictors of carbon content than clay content or dithionite‐extractable iron. SSA explained 55 % and CEC 54 % of the variation in carbon content. The carbon loadings of the soils were between 0.57 and 1.06 mg C m^—2, and therefore in the ”︁monolayer equivalent” (ME) level. The increase in SSA after carbon removal (ΔSSA) was significantly and positively related to carbon content (r² = 0.77). Together with CEC of carbon‐free samples, ΔSSA explained 90 % of the variation in carbon content. Clay (< 2 μm) and fine silt fractions (2—6.3 μm) contained 68—82 % of the bulk soil organic carbon. A significantly positive relationship between carbon content in the clay fraction and in the bulk soil was observed (r² = 0.95). The carbon pools of the clay and fine silt fractions were characterized by differences in C/N ratio, δ¹³C ratio, and enrichment factors for carbon and nitrogen. Organic matter in clay fractions seems to be more altered by microbes than organic matter in fine silt fractions. The results imply that organic matter accumulates in the fractions of smallest size and highest surface area, apparently intimately associated with the mineral phase. The amount of cations adhering to the mineral surface and the size of a certain and specific part of the surface area (ΔSSA) are the mineral phase properties which affect the content of the organic carbon in loess derived arable surface soils in Central Germany most. There is no monolayer of organic matter on the soil surfaces even if carbon loadings are in the ME level.     

Influence of tillage and rotation systems on distribution of organic carbon associated with particle-size fractions in Chernozemic soils of Saskatchewan, Canada

The effects of several dominant tillage and rotation systems on soil organic C content of different particle-size fractions were studied in Chernozemic soils from southwestern and east-central Saskatchewan, Canada. In an Orthic Brown Chernozem in southwestern Saskatchewan, 7 years of no-till cereal–fallow, imposed on a long-term tillage fallow–wheat rotation soil, resulted in 0.1 Mg C ha⁻¹ more organic C mass in the sand + organic matter (OM) fraction of the 0- to 5-cm layer, whereas organic C associated with coarse silt (CS), fine silt (FS), coarse clay, and fine clay of 0- to 5- and 5- to 10-cm layers was less than that of the comparable tilled cereal–fallow system. Conversion of tilled fallow–wheat rotation soil to continuous cropping had a slight effect, whereas the organic C mass in all the size fractions was significantly increased in both 0- to 5- and 5- to 10-cm layers after alfalfa was introduced on tilled fallow–wheat as perennial forage for 10 years. In an Orthic Black Chernozem in east-central Saskatchewan that was cultivated and tilled using a cereal–fallow rotation for 62 years, organic C mass decreased in sand + OM, CS, and FS of 0- to 10-cm depth. Conversion of the tilled cereal–fallow cropland soil back to seeded grassland resulted in significantly more soil organic C in sand + OM fraction after 12 years of grass seed-down. The sand + OM fraction appears to be the size fraction pool initially most sensitive to adoption of management practices that are liable to sequester carbon in the soil.     

Effects of mineral surface iron on the CPMAS 13C-NMR spectroscopic detection of organic matter from soil fractions in an agricultural topsoil with different amendments

The decrease of NMR visibility of the C signal in soil samples due to the association between organic carbon (OC) and the topsoil mineral surface was investigated. CPMAS ¹³C‐NMR spectra were obtained for soil particle‐size fractions (< 2 μm, 2–20 μm, > 20 μm) and bulk soils from an agricultural topsoil (Chernozem) that had received three different amendments (no fertilization, mineral fertilization (NPK), mineral (NPK) and organic (cattle manure) fertilizations) at Bad Lauchstädt, Germany. The soil organic carbon content of the three soils depended on the degree of soil fertilization. There was no constant relationship between the total NMR signal intensity and the total amount of organic carbon (TOC) for all size fractions. Indeed, a key role played in the C signal intensity by the paramagnetic ferric ion from the clay content in soil fractions and bulk soils was confirmed. Thus, we describe the variations of C signal intensity by taking into account the distribution of clay‐associated OC and non‐associated OC pools. Depending on the amendment, the C signal visibility was weakened by a factor of 2–4 for the clay‐associated OC. This estimation was rendered possible by combining mineral specific surface area (SSA) measurements with the N₂ gas adsorption method (BET method) and determination of TOC and iron concentrations. This approach contributes to the quantitative evaluation of the CPMAS ¹³C‐NMR detection.     

Soil organic carbon distribution drives microbial activity and functional diversity in particle and aggregate-size fractions

Chemical and functional characterizations of particle-size and aggregate fractions of soils were performed to investigate whether accessibility and decomposability of organic matter regulate functions and diversity of the soil microbial community at the micro-habitat scale. Soils were physically fractionated into particle size fractions, free-particulate organic matter (F-POM), macro-aggregates (250–2000 μm) and micro-aggregates (53–250 μm). Organic C was enriched in silt and clay, micro-aggregates and F-POM fractions. Enzymes showed the greatest activity in the fine fractions (silt and clay) and F-POM, and were largely influenced by organic C content. MicroResp-CLPP (Community Level Physiological Profile) showed the lowest catabolic responses in the sand and the highest in the fine fraction and micro-aggregates. In general, organic C availability drove soil activity and functional diversity: soils with the higher amount of organic C showed the higher catabolic activity. However, this response was variable within soil fractions, where organic C accessibility, as well as microbial selection and distribution, affected functional diversity.     

Losses of carbon and nitrogen with prolonged arable cropping from sandy soils of the South African Highveld

A knowledge of the kinetics of organic matter transformations in arable soils is important for managing them sustainably. Our aim in this study was to elucidate the effects of cropping period on pools of C and N in coarse‐textured savanna soils of the South African Highveld. Composite samples were taken from the top 20 cm of soils (Plinthustalfs) that have been cropped for lengths of time varying from 0 to 98 years in each of three different agro‐ecosystems in the Free State Province of South Africa. Thereafter, soil organic C and N concentrations were determined in the bulk soil (< 2 mm) as well as in the clay (< 2 μm), silt (2–20 μm), fine sand (20–250 μm), and coarse sand (250–2000 μm) separates. Long‐term cultivation of native grassland reduced soil C and N concentrations by 65 and 55%, respectively. Losses of soil organic matter occurred from all particle‐size separates, although rate loss constants increased as particle size increased. The concentrations of organic C reached equilibrium after 34 years for the bulk soil and after 55 years for clay‐size separates. Nevertheless, organic matter attached to silt continued to be lost as the cropping continued, probably due to wind erosion. Changes in soil properties thereby continued even after almost 100 years of cultivation.     

Distribution of polycyclic aromatic hydrocarbons in particle‐size separates and density fractions of typical agricultural soils in the Yangtze River Delta,east China

Soil organic matter can be divided into different organic carbon (C) pools with different turnover rates. The organic pollutants in soils associated with these organic C pools may have different bioavailability and environmental risks during the decomposition of soil organic matter. We studied the distribution patterns of 15 USEPA priority polycyclic aromatic hydrocarbons (PAHs) in different particle‐size separates (clay, fine silt, coarse silt, fine sand and coarse sand) and density fractions (light and heavy fractions) of nine agricultural topsoils (0–20 cm depth) from a contaminated area in the Yangtze River Delta region of east China. There was a decreasing trend in PAH concentration in particle‐size separates with decreasing particle size. However, the different particle‐size separates had similar PAH composition. The concentration of PAHs in the light fraction ranged from 13 037 to 107 299 μg kg^?1, far higher than in the heavy fraction, which ranged from 222 to 298 μg kg^?1. Although the light fraction accounted for only 0.4–2.3% of the soils, it was associated with 31.5–69.5% of soil PAHs. The organic matter in coarse silt had the strongest capacity for enrichment with PAHs. Combining the distributions of PAHs and the turnover rates of organic matter in different soil fractions, the environmental risks of PAH‐polluted soils may be due mainly to the PAHs associated with sand and the light fraction.     

Nitrogen distribution and 15N natural abundances in particle size fractions of a long‐term agricultural field experiment

The present study combined a physical fractionation procedure with the determination of the natural abundance of ¹⁵N to investigate the impact of organic manure and mineral fertilizer application, and fallow on changes of N associated with different soil particle size fractions. The long‐term field experiment was conducted since 1956 in Ultuna, Sweden, on an Eutric Cambisol. Nitrogen in bulk soil and in particle size fractions changed significantly since 1956. The N_t concentrations in bulk soil decreased in all treatments not receiving organic materials. Comparing the N contribution of particle‐size fractions to the total N amount revealed the following ranking: silt > clay > fine clay > fine sand > coarse sand. The relative contribution of N in silt sized particles significantly increased from low to high bulk soil N contents, whereas N in clay and fine clay fractions decreased. The C : N ratios of particle size fractions differed considerably more between treatments than C : N ratios in bulk soils. Generally, the C : N ratios decreased from coarse to fine fractions emphasizing the tendency of smaller fractions being more significant as N sink than as C_org sink. ¹⁵N abundances varied more between particle size fractions of single treatments than between bulk soil from differently treated plots. Within treatments we observed differences of up to 7.1 ‰ between particle size fractions. In most cases δ ¹⁵N values increased with decreasing particle sizes. This pattern on average was similar to changes in δ ¹³ C. Our results suggest that silt sized particles acted as medium‐term sink of introduced N and that ¹⁵N abundances in particle size fractions sensitively reflect changes in N status in response to soil management.     

Nitrogen in particle size fractions of soils incubated for five years with 15N-ammonium and 14C-hemicellulose

Four soils with a range of clay and silt contents were incubated for 5 a with ¹⁵N-labelled (NH₄)SO₄ and ¹⁴C-labelled hemicellulose and then fractionated according to particle size by ultrasonic dispersion and sedimentation. The distribution of labelled and native N between clay, silt and sand fractions was determined and elated to previous results on the C distributions. Between 29% and 48% of the added N was found in organic form. The ¹⁵N atom percentage excess decreased in the order: clay > whole soil > silt > sand. For both clay and silt, the enrichment factor for labelled and native N decreased with increasing fraction weight. Clay enrichment was higher for labelled than for native N, the converse being true for silt. The distribution of whole soil labelled organic N was: clay 77–91%, silt 4–11%, and sand <0.5%. Corresponding values for native N were 69–74%, 16–22%, and 1–2%, respectively. All soils had higher proportions of labelled than of native N in the clay, the converse was true for the silt. The C/N ratio of the native silt organic matter was higher and that of clay organic matter lower than whole soil C/N ratios. Differences between the C/N ratio distributions of native and labelled organic matter were small. The relative distribution of labelled N and C was very similar confirming that the turnover of C and N in soil organic matter is closely interrelated.     

Effects of manure application on carbon,nitrogen, and carbohydrate contents of a silt loam and its particle-size fractions

Summary Changes in the organic matter and carbohydrate contents of a silt loam and of its particle-size fractions were examined after 10 years of applying solid cattle manure. Relative to the unmanured soils, bi-annual applications of 40 and 80 Mg ha^-1 manure increased C, N, and total carbohydrate contents of the whole soil and of all the particle-size fractions. The manure application had no effect on the composition of the carbohydrates. However, the organic matter of the soil with the high application rate (80 Mg ha^-1) was enriched in carbohydrates. The distribution of carbohydrates in the particlesize fractions and the ratio (Ga+Ma):(Ar+Xy) indicated that carbohydrates of both plant and microbial origin were increased upon application of manure to the soil.     

Mikrobiologische Beurteilung biologischer Bodendekontaminationsverfahren im Modellversuch

Microbiological control of the biological decontamination of soils Five agricultural soils which were contaminated with n-tetradecane, 5-methyl-3-heptanone and naphthalene were used to follow the biological clean-up process of these soils. The hydrocarbon content, the microbial biomass (substrate-induced respiration), dehydrogenase activity and N-mineralisation were monitored for 20 weeks in the contaminated and control soils. Dehydrogenase activity followed similar patterns within various soils, the values being lowest at the beginning of the experiment and highest during the following weeks. N-mineralisation of four contaminated soils was reduced at the start of the experiment, but was increased afterwards. The influence of contamination on microbial activities was detected even after elimination of the hydrocarbons. The addition of compost of bark and pieces of bark increased the biological cleanup process of the Calcic Chernozem contaminated with 3,8% (w/w) fuel oil. The addition of both organic materials reduced the decontamination rate of the Haplic Chernozem. The ratio of N-mineralisation to microbial biomass of the Calcic Chernozem increased with the addition of compost of bark and pieces of bark, but decreased with the same treatment of the. Haplic Chernozem. The results of this study suggest that microorganisms immobilize nitrogen in the Haplic Chernozem ammended with pieces of bark to a great extent during the whole experiment. The high N-immobilization and the adsorption of hydrocarbons to the added organic material may be the main cause of the slow decontamination of the Haplic Chernozem.     

Influences of organic fertilization and solarization in a greenhouse on particle-size fractions of a Mediterranean sandy soil

 The effects of a composted organic amendment and solarization on the organic matter (OM) of a sandy soil were determined by means of particle-size fractionation and analysis of carbon and nitrogen contents. After 2 years, total soil carbon increased under organic fertilization but did not significantly change with solarization. As a consequence of the climatic conditions in the greenhouse, the carbon concentrations (g kg^–1 fraction) of the particle-size fractions were lower than those found for temperate soils and closer to those for tropical soils. The carbon amounts (g kg^–1 soil) and carbon:nitrogen ratios, which were highest in fractions >200 μm, reflected the short-term influence of the industrially processed organic amendment, rich in composted coarse plant debris. In contrast, the characteristics of the OM associated with each fraction were not significantly affected by solarization. In comparison with other coarse-textured temperate or tropical soils, carbon concentrations in fine silt (2–20 μm) and clay (0–2 μm) fractions were very low. This suggests a "greenhouse effect", together with a high rate of carbon mineralization affecting fine silt and clay fractions. Received: 19 November 1999