模拟移动床技术在生化领域的应用研究进展

模拟移动床技术作为一种新型色谱分离技术,应用日益广泛。文章介绍了模拟移动床技术的发展情况及其工作原理,并详细介绍了模拟移动床技术在生化产品分离中的研究进展和应用情况。     

中试型多功能模拟移动床色谱分离设备的研制

模拟移动床色谱分离技术是一种先进高效的分离手段,我国还处于起步阶段,主要是缺少相关的设备。目前,我国只有极少数的实验室及工业化模拟移动床色谱设备,缺少具有中试能力的多功能色谱设备,因此,在前人研究的基础上,进行了中试型多功能模拟移动床色谱设备的研制。     

黑龙江省农产品加工工程技术研究中心技术简介

一步分离法分离纯化甜叶菊苷工艺 该技术利用超声波提取技术代替传统的甜叶菊苷水浸法,实现了提取时间缩短92％,提取率达到90％的目标;以成熟的模拟移动床色谱分离技术对甜叶菊苷的分离提纯工艺进行改造,达成一道工序完成甜叶菊料液的吸附、脱盐、脱色。     

黑龙江省农产品加工工程技术研究中心宏源分离技术研究所

黑龙江省农产品加工工程技术研究中心宏源分离技术研究所是专业从事模拟移动床色谱（SMBC）分离殴备研制及技术应用研究的专业科研单位,现已具有多项自主知识产权及专利。主要产品有：实验型SMBC、中试型SMBC,并可承担工业化SMBC的设计与制造,同时可提供相应的应用技术,包括动植物活性多糖提驭、分离与纯化,手性药物分离纯化,以及植物化学成分分离纯化等。该类设备与技术达到了国内领先水平,该研究所能够提供最优质的技术服务。     

多维气相色谱及其在中药领域中的研究进展

多维气相色谱是近年来发展较为迅速的一种色谱分离技术,其高分辨、高效、高峰容量以及易与质谱等其他技术联用等优势正成为研究应用的热点。本文对多维气相色谱技术及其近年来在中药研究中的应用进行了综述,并对多维气相色谱的发展趋势和前景进行了展望。     

模拟移动床分离纯化纳豆激酶的研究

利用模拟移动床从纳豆激酶发酵液中分离纯化纳豆激酶,通过单柱试验计算出模拟移动床的分区方式及初始参数,利用正交试验方法优化参数,最终所得纳豆激酶的纯度为90%,收率为85%,活性为(8 000±50)IU/mg。     

高效液相色谱法在蛋白质分离检测中的应用

综述高效液相色谱法在蛋白质分离检测中的应用,概括分析了反相分配色谱、亲和层析、离子交换色谱、分子排阻色谱和疏水色谱等几种常用的蛋白质色谱分离模式,以及相应的检测技术,并展望了该法在蛋白质分离分析方面的应用。     

连续色谱移动床分离甘蔗渣提取物制备阿拉伯糖和木糖的研究与应用

以PCR-642Ca~(2+)树脂为固定相,纯水为流动相,使用连续色谱分离移动床对甘蔗渣木糖母液中的L-阿拉伯糖和D-木糖进行了分离,探讨了Ⅰ区、Ⅱ区、Ⅲ区、Ⅳ区流量、进料流速、进料质量分数等操作条件对连续移动床色谱分离效果的影响。结果表明,最佳工艺参数为进料质量分数45%,进料流速8.5 mL/min,L-阿拉伯糖出口流速11.5 mL/min,木糖出口流速14 mL/min。在此条件下,L-阿拉伯糖出口质量分数为9.46%,纯度达92.29%,收率达92.84%;D-木糖出口质量分数为21.03%,纯度达91.46%,收率达90.67%。为寻找一条利用甘蔗渣木糖母液等富含D-木糖、L-阿拉伯糖的料液生产L-阿拉伯糖和D-木糖的高效、环保、成本低、易于实现的生产工艺提供了参考。     

高速逆流色谱实验在有效成分分离中溶剂体系的选择

高速逆流色谱(HSCCC)是一种新型液—液分配色谱技术,在中药、生化、保健食品、天然产物化学、环境分析等领域广泛应用,溶剂体系是其分离纯化效果的关键,对HSCCC有效成分分离时,溶剂体系的选择规律及筛选方法进行了总结,为筛选快速、便捷的高速逆流色谱溶剂体系提供了可靠的理论基础,并展望了该领域的研究及应用前景。     

基于液-液萃取机理的新型环境样品前处理方法研究进展

新型环境样品前处理技术已经逐步取代传统液-液萃取。液相微萃取、液膜萃取和逆流色谱技术均是集采样、萃取和浓缩于一体的环境友好型环境样品前处理技术。液相微萃取、液膜萃取实质上是微型化的液-液萃取;逆流色谱结合了液液萃取和分配色谱的优点,是一种新型色谱分离技术。3种技术均对环境样品中的痕量/超痕量污染物质有高效的富集、净化效果。笔者综述了液相微萃取、液膜萃取和逆流色谱技术的优缺点及在环境样品前处理中的应用。     

不同施肥条件对玉米生长季耕层土壤微生物量碳的影响

为了研究不同施肥条件下棕壤长期定位试验玉米整个生长季微生物量碳含量的动态变化,试验采用氯仿熏蒸、灭菌、提取,TOC测定的方法研究了15个不同施肥处理对耕层土壤微生物量碳含量的影响。结果表明,施用有机肥料可以显著提高土壤微生物量碳含量,随着有机肥用量的增加土壤微生物量碳提高,在玉米的生殖生长阶段其含量达到最大。单施化肥区土壤微生物量碳含量在玉米整个生长季节都处于一种波动变化,在玉米前期,其含量受温度、水分、作物吸收等影响较大;而到了生殖生长阶段,微生物量碳含量有逐渐升高趋势,但上升幅度不大;成熟期微生物量碳含量迅速下降,都低于播种前。     

不同基因型饲用甜菜生长动态研究

为解决畜草失衡,机械化、规模化发展饲用甜菜提供理论依据。对5个不同基因型饲用甜菜品种生长指标及根体产量和锤度进行动态研究,并对根体产量和锤度与8个主要指标进行灰色相关度分析。结果表明：叶柄厚度和产量是影响锤度的主导因素；株高和幼苗百株重是饲用甜菜产量的主导因素；叶片数作为饲用甜菜一项重要指标,他不仅影响根体锤度,也影响根体的产量。多倍体品种在改良饲用甜菜苗期抗逆性和提高根体含糖量过程中有很高的利用价值。‘饲用3号’综合指标表现较为突出,并且产量在参试品种中位居第一(12034.56 kg/亩),具有很高的推广价值。     

Pressure Sweep Adsorption Processes for the Concentration of Methane from Coal-bed Gas Extracted

The coal-bed gas was usually extracted from mine in order to avoid coal gas outburst.The Methane(CH4),a main component in coal-bed gas,is a kind of excellent gas fuel and raw material for chemical industry.China is very rich in the resource of coal bed gas,however,most of the coal bed gas extracted from mine is wasted because of the too low concentration of methane. Pressure sweep adsorption(PSA) technique is noticeable among the methods for the concentration of methane from coal-bed gas.However,there are a few studies about this field.Studies on the pressure sweep adsorption(PSA) process for the separation of CH4/N2 using actived carbon as adsorbent are summarized.The thermodynamic relationships and kinetic processes and the influence of the operate parameters on the concentration of methane are investigated.These results have a direct application in coal-bed gas.Some suggestions for the problems need to be solved in PSA process for the separation of methane are also proposed.     

Automatic Mechanical Transmission Test Bed and Development of Testing-controlling Program

Automatic mechanical transmission (AMT) bed testing is one of the important jobs for AMT researching and development. Through bed testing we can finish many works such as testing performance of AMT, AMT-engine matching, optimizing control rules and so on. AMT testing bed is built up base on the sample AMT developed by ourselves. Data are transmitted between PC and SCM though RS232 COM. By this way electronic throttle, clutch and shifting are controlled. Hardware port can be read in Windows 2000 by calling the unpublicized functions. Testing-controlling Program is also developed and AMT bed testing can be carried out with advanced test and control technology.     

膜分离技术在乳品工业中的应用

介绍了膜分离技术在乳品工业中的应用范围,同时从理论上阐述了膜分离技术微滤、超滤、纳滤与反渗透在乳品除菌、乳蛋白质的分离浓缩、分离脂肪、乳清脱盐、牛奶浓缩、乳品标准化,以及乳品生产企业废水回收等方面的应用。     

Application of Membrane Separation Technologyin Water & Wastewater Treatment and Its Relative Priority

The application of membrane separation technology in water and wastewater field is introduced in this paper. It is demonstrated that membrane separation technology is the future of the development of water and wastewater treatment, as well as water industry, compared with conventional treatment methods from three aspects: technology, economy and operation and management, the relative priority of the membrane is discussed.     

食品新技术在果胶制备中的应用

简述了微波萃取技术、树脂吸附技术、酶技术、膜分离技术、真空冷冻干燥技术等若干食品加工新技术在果胶制备中的应用,同时对上述技术进行进一步展望.     

Performance analysis of StimGun technology

With further exploration and development of oil and gas wells, it is found that the unconventional oil-gas bed occupies a major proportion in gas reserves. However, it is difficult to obtain satisfactory control by using conventional perforation technology because of its characteristics of low pore, low permeability and compact lithology in the unconventional oil-gas bed. The StimGun tech (synergize compound perforation) is described as following: after the perforating gun is fired, propellant burns with high-energy gas produced. Then the high-energy gas enters the perforating hole and forms multi-path cracks around perforated hole, which connects with the natural cracks and improves the permeability of oil and gas bed. The application and advantage of StimGun are introduced. The Stimgun is successfully applied in Sichuan LG oilfield. It can control the fracture heights effectively and remove contamination formation with no water producing, and it is also a great help to increase productivity of the oil-gas bed with low permeability.