Characterization of starches of proso,foxtail, barnyard,kodo, and little millets

Scanning electron microscope (SEM) pictures of small millet starch granules showed more large polygonal and few small spherical or polygonal granules. The granules of small millets resembled those of rice starch granules. The size of the starch granules ranged from 0.8–10 m. The size of the granules was larger in barnyard millet and smaller in proso millet. Several granules showed deep indentation caused by protein bodies. SEM of starch isolated from 24 hour-germinated kodo millet showed pitting or pinholes at some points due to the attack of amylases (preferentially on bigger granules). Brabender viscoamylograph studies on small millet starches revealed that the gelatinization temperatures ranged from 75.8 to 84.9 ^° C. Barnyard millet possessed lower amylograph viscosity, minimum breakdown, and relative breakdown values when compared to the other small millets.     

Effects of application of nitrogen fertilizer on concentrations of P, K, S, Ca, Mg, Na, Cl, Mn, Fe, Cu and Zn in perennial ryegrass/white clover pastures in south-western Victoria, Australia

Effects of timing and rate of N fertilizer application on concentrations of P, K, S, Ca, Mg, Na, Cl, Mn, Fe, Cu and Zn in herbage from perennial ryegrass/white clover pastures were studied at two sites in south-western Victoria, Australia. Nitrogen fertilizer (0, 15, 25, 30, 45 and 60 kg ha^–1) was applied as urea in mid-April, early May, mid-May, early June and mid-June 1996 to pastures grazed by dairy cows. At Site 1, N fertilizer resulted in a linear increase in P, K, S, Mg and Cl concentrations in herbage and a linear decrease in Ca concentration. For all times of application, concentrations of P, K, Ca, Mg and Cl in herbage increased by 0·0048, 0·08, −0·010, 0·0013 and 0·053 g kg^–1 dry matter (DM) per kg N applied respectively. For S concentration, maximum responses occurred in mid-May (0·012 g kg^–1 DM per kg N applied). At Site 2, N fertilizer resulted in a linear increase in P, S and Na concentrations in herbage, a linear decrease in Ca concentration and a curvilinear increase in K and Cl concentration. The maximum responses for P, S and K concentrations in herbage occurred for the N application in mid-June and were 0·015, 0·008 and 0·47 g kg^–1 DM per kg N applied respectively. For Cl concentration, the maximum response occurred for the N application in early June and was 0·225 g kg^–1 DM per kg N applied. Overall, applications of N fertilizer up to 60 kg ha^–1 did not alter herbage mineral concentration to levels that might affect pasture growth or animal health.     

Evaluation of the enzyme-amplified ELISA for the detection of potato viruses A,M, S,X, Y,and leafroll

Various parameters,e.g. types of microtiter plate for DAS-ELISA (double antibody sandwich-enzyme-linked immunosorbent assay), use of fresh or frozen amplifier solutions for enzyme-amplified-ELISA, and use of sodium diethyldithiocarbamate (NaDIECA) in sample buffer in cocktail-ELISA were evaluated for the detection of potato viruses A, M, S, X, Y and leafroll from potato foliage. Dynatech Immulon immunoplates provided higher readings for all viruses. Fresh amplifier solution in amplifed-ELISA was superior to frozen solutions. Amplified ELISA gave only marginal improvement in the sensitivity over the standard DAS-ELISA. Addition of NaDIECA in sample buffer did not improve the detection of viruses in DAS-, amplified-, or cocktail-ELISA. Cocktail-ELISA can reduce antigen incubation time to as short as 15 min for PVA, PVM and PVX; 1 hr for PVY and PLRV; and 2–4 hr for PVS using pre-coated plates. Although amplified-ELISA is slightly more sensitive than DAS-ELISA for certain potato viruses, it is not suitable for large-scale indexing of potato viruses in Seed Certification Laboratories, in view of the additional steps needed in carrying out this procedure.     

Study of antimicrobial activity of aloevera, chitosan, and curcumin on cotton, wool, and rabbit hair

Aloevera, chitosan, and curcumin were applied in alone and in combination with each others on cotton, wool and rabbit hair by exhaustion method for the assessment of their antimicrobial activity. The antimicrobial activity of these natural ingredients was better in peroxide treated cotton, formic acid treated wool/rabbit hair fibrous substrates than their corresponding intact ones. Aloevera shown better antimicrobial activity than chitosan and curcumin when applied alone and its antimicrobial activity was enhanced by addition of both chitosan and curcumin. The application of aloevera+chitosan+curcumin combination on peroxide treated cotton and formic acid treated wool/rabbit hair fibrous substrate was fast up to twenty five washing cycles.     

Milling,functional and thermo-mechanical properties of wheat,rye, triticale,barley and oat

The milling potential of hulled barley, hulled oat, triticale, rye and wheat was studied using a long tempering process and a laboratory four-roller mill. Regardless of the investigated cereal, the results indicated a significant influence (p < 0.05) of volume per surface area ratio on the milling yield and ash contents of the flour. The lowest milling yield was obtained in case of hulled oat. Solvent retention capacity profiles were determined for all investigated whole cereals and flours for predicting the contribution of different polymers to the functionality of samples. For all solvents higher values were obtained for the whole cereals compared to the corresponding flour. Thermo-mechanical properties of the whole cereals and refined flours were also investigated. If in case of wheat the gluten proteins play an essential role on dough behaviour during kneading at 30 °C, in case of triticale, rye, hulled barley and hulled oat, the fibers play a major role as well. Thermo-mechanical properties of starch registered a large variation between cereals and/or flours. The lowest torque value corresponding to starch gelatinization (C3) was registered in case of the hulled oat flour, 1.92 Nm, while the highest value in case of rye flour, 2.65 Nm.     

Response of permanent and reseeded grassland to fertilizer nitrogen. 2. Effects on concentrations of Ca, Mg, K, Na, S, P, Mn, Zn, Cu, Co and Mo in herbage at a range of sites

The concentration of mineral elements in herbage from permanent swards of mixed species composition (predominantly Agrostis spp., Holcus lanatus, Festuca rubra, Lolium perenne, Poa spp., etc.) was compared with that from sown perennial ryegrass ( Lolium perenne ) at two fertilizer rates (0 and 300kg N ha⁻¹ year⁻¹, both with P and K) and with L. perennel Trifolium repens (at 0 N with P and K). The investigation was conducted over four years at sixteen contrasting sites in England and Wales. Herbage samples for mineral analysis were taken at first and fourth harvests (May and August) each year from plots under 4-weekly cutting. There was a wide degree of variation between sites, particularly for concentrations of Ca, Mg, Na and all trace elements analysed. Herbage from L. perenne reseeds had significantly higher concentrations of Ca, Mg, Na, Co and S, but K, Mo, Zn, Cu and P were generally lower than in the permanent swards. L. perennel T. repens swards had the highest concentration of major cations, but trace element levels were generally intermediate between those for 0 N permanent and L. perenne swards. Fertilizer N reduced concentrations of Ca, Mn, Mo and S, and increased Mg, Na and Zn, with no consistent effects on K, Co and Cu. Herbage at harvest 4, compared with harvest 1, had higher Ca, Mg, Na, Mn, Zn and S. Permanent and L. perenne swards responded similarly to fertilizer N and between harvest dates. Results are discussed in relation to previous reports of botanical and fertilizer effects on mineral concentrations, and the mineral requirements of livestock. Changes in mineral concentration resulting from reseeding permanent swards are considered unlikely to increase mineral-related livestock disorders, and in many cases could be beneficial, though problems might arise on sites where some elements, e.g. Cu and Mg, are low.     

Chemical fractionation of seven heavy metals (Cd, Cu, Fe, Mn, Ni, Pb, and Zn) in selected paddy soils of Iran

The content and fractionation of seven heavy metals (Cd, Cu, Fe, Mn, Ni, Pb, and Zn) were determined in 28 surface soil samples (0–20 cm) of agricultural topsoil from Isfahan Province in central Iran. The order of abundance of metals in the soils was Fe (1240.4 mg kg^?1) > Mn (95.7 mg kg^?1) > Pb (51.6 mg kg^?1) > Zn (23.8 mg kg^?1) > Ni (13.4 mg kg^?1) > Cu (7.0 mg kg^?1) > Cd (2.8 mg kg^?1). Iron, Mn, Ni, Pb, and Zn existed in paddy soils mainly in Fe-Mn oxides (53.6 %, 65.2 %, 40.4 %, 40.8 %, 53.3 %, respectively), whereas Cu and Cd occurred essentially as residual mineral phase (41.4 %) and carbonate (36.1 %), respectively. The mobile and bioavailable fractions of Cd, Cu, Fe, Mn, Ni, Pb, and Zn in paddy soils averaged 48.8, 20.8, 0.79, 29.2, 28.5, 41.1, and 24.8 %, respectively, which suggests that the mobility and bioavailability of the seven metals probably decline in the following order: Cd > Pb > Mn ≥ Ni > Zn > Cu ? Fe, suggesting greater contribution of anthropogenic Cd. As Cd in soil is easily accumulated by plants through the root system, the concentration of Cd in these paddy soils could be a concern to human health.     

酸碱条件对枯草芽胞杆菌FJAT-14254代谢物产生的影响

为考察酸碱条件对枯草芽胞杆菌FJAT-14254代谢物产生的影响,采用气相色谱-质谱联用分析枯草芽胞杆菌FJAT-14254菌株的胞内成分。结果表明：通过谱库扫描得到32种高匹配率代谢物的初步鉴定结果,主要包括氨基酸类、酸类、烷烃类等,其中含量相对较高的有7种;枯草芽胞杆菌FJAT-14254代谢物的种类和含量与其培养环境的酸碱度变化呈一定的相关性,强酸性(pH＝3)条件下培养的胞内代谢物具有特异性,而接近中性(pH＝5、7)条件及碱性(pH＝9、11)条件下培养胞内代谢物则相似。由此推断,不同pH条件影     

Structure Elucidation of New Acetylated Saponins,Lessoniosides A,B, C,D, and E,and Non-Acetylated Saponins,Lessoniosides F and G,from the Viscera of the Sea Cucumber Holothuria lessoni

Sea cucumbers produce numerous compounds with a wide range of chemical structural diversity. Among these, saponins are the most diverse and include sulfated, non-sulfated, acetylated and methylated congeners with different aglycone and sugar moieties. In this study, MALDI and ESI tandem mass spectrometry, in the positive ion mode, were used to elucidate the structure of new saponins extracted from the viscera of H. lessoni. Fragmentation of the aglycone provided structural information on the presence of the acetyl group. The presence of the O-acetyl group was confirmed by observing the mass transition of 60 u corresponding to the loss of a molecule of acetic acid. Ion fingerprints from the glycosidic cleavage provided information on the mass of the aglycone (core), and the sequence and type of monosaccharides that constitute the sugar moiety. The tandem mass spectra of the saponin precursor ions [M + Na]⁺ provided a wealth of detailed structural information on the glycosidic bond cleavages. As a result, and in conjunction with existing literature, we characterized the structure of five new acetylated saponins, Lessoniosides A–E, along with two non-acetylated saponins Lessoniosides F and G at m/z 1477.7, which are promising candidates for future drug development. The presented strategy allows a rapid, reliable and complete analysis of native saponins.     

氮磷钾肥对稻米铁、锌、铜、锰、镁、钙含量和产量的影响

 采用田间试验，在四川省西昌市用两个水稻品种研究了氮、磷、钾肥施用量对稻米中铁、锌、铜、锰、镁、钙含量和产量的影响。结果表明，稻米中铁、锌、铜、锰、镁、钙含量均随着施氮量增加先上升后下降，滇屯502的铁、锌、铜、锰、镁、钙含量和产量都以施用90 kg/hm2 N最高，稻谷产量以施用180 kg/hm2 N最高；而合系39的铁、锌、铜、锰、镁、钙的含量以施用180 kg/hm2 N最高，稻谷产量以施用270 kg/hm2 N最高，说明供试籼型品种滇屯502对氮肥的敏感性较粳型品种合系39强；磷肥明显降低了稻米中铁、铜、锰、钙的含量和产量，适量增施磷肥有利于增加稻米中镁的含量和产量；适量施用钾肥有利于提高稻米中铁、锌、铜、锰含量和产量，两供试品种铁、锌、铜、锰含量均以90 kg/hm2 K2O时最高，而钾肥明显降低了稻米中镁、钙的含量和产量。     

Selected Uncommon Legumes as a Source of Essential Fatty Acids,Tocopherols, Tocotrienols,Sterols, Carotenoids,and Squalene

Plant Foods for Human Nutrition - The seeds of nine species belonging to the Fabaceae family (Cassia alata, Cassia uniflora, Cassia obtusifolia, Cassia tora, Crotalaria albida, Crotalaria juncea,...     

云南元谋县葡萄品种应用现状及存在问题分析

介绍元谋县葡萄种植发展优势、品种应用现状、优势品种及存在的问题,并提出相应对策,为元谋县葡萄品种的发展提供参考。     

Antimicrobial activities of Allium sativum,Allium cepa,Raphanus sativus,Capsicum frutescens,Eruca sativa,Allium kurrat on bacteria

The antimicrobial activities of the crude juices ofAllium sativum, Allium cepa, Raphanus sativus have been found to be strongly active onEscherichia coli, Pseudomonas pyocyaneus, Salmonella typhi andBacillus subtilis, while the crude juices ofCapsicum frutenscens andEruca sativa were active only onEscherichia coli, Salmonella typhi andBacillus subtilis. The crude juice ofAllium kurrat was active onEscherichia coli andBacillus subtilis. The active antibacterial substances were easily isolated by different organic solvents.

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Zusammenfassung Die antimikrobiellen Aktivitäten der rohen Säfte vonAllium sativum undAllium cepa wurden als stark befunden gegenEscherichia coli, Pseudomonas pyocyaneus, Salmonella typhi undBacillus subtilis, während die rohen Säfte vonCapsicum frutescens undEruca sativa nur gegenEscherichia coli undBacillus subtilis aktiv waren. Der rohe Saft vonAllium kurrat erwies sich als aktiv gegenEscherichia coli undBacillus subtilis. Die aktiven antimikrobiellen Substanzen waren durch verschiedene organische Lösungsmittel leicht zu isolieren.

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Résumé Les activités antimicrobiennes des jus crusd'Allium sativum, Allium cepa, Raphanus sativus ont été trouvées actives en affectantEscherichia coli, Pseudomonas pyocyaneus, Salmonella typhi etBacillus subtilis, mais les jus crus deCapsicum frutescens etEruca sativa affectent seulementEscherichia coli, Salmonella typhi etBacillus subtilis. Le jus crud'Allium kurrat affecteEscherichia coli etBacillus subtilis. L'active substance antibactérienne est isolée facilement par dissolvants organiques différents.